Journal of Korea Technical Association of The Pulp and Paper Industry (펄프종이기술)

Korea Technical Association of the Pulp and Paper Industry (한국펄프종이공학회)
권호 표지
DOI QR코드

DOI QR Code

Impact of sodium or potassium cations in culture medium to ethanol fermentation by Saccharomyces cerevisiae

배양액내 나트륨 및 칼륨 이온 농도가 Saccharomyces cerevisiae의 발효에 미치는 영향

  • Song, Woo-Yong (Department of Wood and Paper Science, Chungbuk National University) ;
  • Seung, Hyun-A (Department of Biochemistry, Chungbuk National University) ;
  • Shin, Soo-Jeong (Department of Wood and Paper Science, Chungbuk National University)
  • 송우용 (충북대학교 목재종이과학과) ;
  • 성현아 (충북대학교 생화학과) ;
  • 신수정 (충북대학교 목재종이과학과)
  • Received : 2014.12.05
  • Accepted : 2015.02.05
  • Published : 2015.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

In bioethanol from acid hydrolysis process, neutralization of acid hydrolyzate is essential step, which resulted in dissolved cations in glucose solution. Impact of cations to Saccharomyces cerevisiae in glucose solution was investigated focused on ethanol fermentation. Both potassium and sodium cations decreased the ethanol fermentation and glucose to ethanol conversion as potassium or sodium cations. In sodium cation, more than 1.13 N sodium cation in glucose solution led to ethanol production less than theoretical yield with severe inhibition. In 1.13 N sodium cation concentration, ethanol fermentation was slowed down to reach the maximum ethanol concentration with 48 h fermentation compared with 24 h fermentation in control (no sodium cation in glucose solution). In case of potassium cation, three different levels of potassium led to silimar ethanol concentration even though slight slow down of ethanol fermentation with increasing potassium cation concentration at 12 h fermentation. Sodium cation showed more inhibition than potassium cation as ethanol concentration and glucose consumption by Saccharomyces cerevisiae.

Keywords

References

  1. Shah, Y.R., and Sen, D.J., Bioalcohol as green energy - A review, International Journal of Recent Scientific Research, 1(2):57-62 (2011).
  2. Balat, M., Production of bioethanol from lignocellulosic materials via the biochemical pathway: A review, Energy Conversion and Management, 52:858-875 (2011). https://doi.org/10.1016/j.enconman.2010.08.013
  3. Shin, S.-J., Han, S.-H., Cho, N.-S. and Park, J.-M., Relationship between biomass components dissolution(xylan and lignin) and enzymatic saccharification of several ammonium hydroxide soaked biomass, Journal of Korea TAPPI 42(1):35-40 (2010).
  4. Alvira, P., Tomas-Pejo, E., Ballesteros, M. and Negro, M.J., Pretreatment technologies for an efficient bioethanol production process based on enzymatic hydrolysis: a review, Bioresource Technology 101:4851-5861 (2010). https://doi.org/10.1016/j.biortech.2009.11.093
  5. Shin, S.-J., Park, J.-M., Cho, DH, Kim YH, and Cho, N.-S., Acid hydrolysis characteristics of yellow poplar for high concentration of monosaccharides production, Journal of Korean Wood Science and Technology 37:578-584 (2009).
  6. Huang, C., Zhu, D.H, Wu, H., Lou, W.-Y. and Zong, M.-H., Evaluating the influence of inhibitors present in lignocellulosic hydrolysates on the cell membrane integrity of oleaginous yeast Trichosporon fermentans by flow cystometry, Process Biochemistry 49:395-401 (2014). https://doi.org/10.1016/j.procbio.2013.12.007
  7. Cho, DH, Shin, S.-J., Bae, Y., Park, C., and Kim, YH., Enhanced ethanol production from deacetylated yellow poplar acid hydrolysate by Pichia stipitis, Bioresource Technology 101:4947-4951 (2010). https://doi.org/10.1016/j.biortech.2009.11.014
  8. Han, S.-H., Cho, DH,, Kim, YH, and Shin, S.-J., Biobutanol production with 2-year old willow biomass by acid hydrolysis and ABE(acetone-butanol-ethanol) fermentation, Energy 61:13-17 (2013). https://doi.org/10.1016/j.energy.2013.04.069
  9. Lee, H., Cho, DH, Kim YH, Shin, S.-J., Kim, SB, Han SO, Lee, J., Kim SW, and Park C., Tolerance of Sacchromyces cerevisiae K35 to lignocellulose-derived inhibitory compounds, Biotechnology and Bioprocess Engineering 16:755-760 (2011). https://doi.org/10.1007/s12257-010-0474-4
  10. Casey, E., Sedlak, M., Ho, N., and Mosier, N., Effect of acetic acid and pH on the co-fermentation of glucose and xylose by a genetically engineered strain of Saccharomyces cerevisiae, FEMS Yeast Research 10:385-393 (2010). https://doi.org/10.1111/j.1567-1364.2010.00623.x
  11. Dean, J.A., Handbook of Chemistry 14th edition, McGraw Hill, New York, pp.5.9-5.21 (1992).
  12. Wei, J.R., Dien, B., Bothast, R., Hendrickson, R., Mosier, N.S., and Ladisch, M.R., Removal of fermentation inhibitors formed during pretreatment of biomass by polymeric adsorbents, Industrial & Engineering Chemistry Research 41:6132-6138 (2002). https://doi.org/10.1021/ie0201056
  13. Olz, R., Larsson, K., Adler, L., and Gustafsson, L., Energy flux and osmoregulation of Saccharomyces cerevisiae grown in chemostats under NaCl stress, Journal of Bacteriology 175:2205-2213 (1993). https://doi.org/10.1128/jb.175.8.2205-2213.1993
  14. Conway, E.J., and Moore, P.T., A sodium-yeast and some of its properties, Biochemistry Journal 57:523-528 (1954). https://doi.org/10.1042/bj0570523
  15. Blumwald, E., Aharon, G.S., and Apse, M.P., Sodium transport in plant cells, Biochimica et Biophysica Acta 1465:140-151 (2000). https://doi.org/10.1016/S0005-2736(00)00135-8
  16. Hosono, K., Effect of salts stress on lipid composition and membrane fluidity of the salt-tolerant yeast Zygosaccharomyces rouxii, Journal of General Microbiology 138:91-96 (1992). https://doi.org/10.1099/00221287-138-1-91
  17. Mavis, R.D., and Stellwagen, E., The role of cations in yeast phosphofructokinase catalyst, The Journal of Biological Chemistry 245:674-680 (1970).
  18. Muntz, J.A., The role of potassium and ammonium ions in alchololic fermentation, The Journal of Biological Chemistry 171:653-665 (1947).
  19. Casey, E., Mosier, N., Adamec, J., Stockdale, Z., Ho, N., and Sedlak, M., Effect of salts on the co-fermentation of glucose and xylose by a genetically engineered strain of Saccharomyces cerevisiae, Biotechnology for Biofules 6:83-92 (2013). https://doi.org/10.1186/1754-6834-6-83
  20. Luisa Neves, M., Oliveira, R.P., and Luca, C.M., Metabolic flux response to salt-induced stress in the halotolerant yeast Debaryomyces hansenni, Microbiology 143:1133-1139 (1997). https://doi.org/10.1099/00221287-143-4-1133