• Journal of Ecology and Environment
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• v.35 no.3
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• pp.195-201
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• 2012

Seasonal variations in the profile and concentrations of essential oil in Zanthoxylum piperitum were investigated by gas chromatography-mass spectrometry. Seasonal changes in the percentages of the main constituents of the essential oil of both leaves and fruits from Z. piperitum varied. Variations in essential oil yield and the amount of monoterpenes and sesquiterpenes in leaves and fruits at different developmental stages were significant. The characteristic content of essential oil in leaves was determined mainly due to the content of monoterpenes, and that in fruits was determined largely due to the sesquiterpenes. Twenty-nine compounds in the oil from Z. piperitum leaves were detected; the major compounds were ${\beta}$-phellandrene (26.90%), citronella (15.32%), ${\beta}$-myrcene (3.24%), ${\alpha}$-pinene (2.79%), trans-caryophyllene (2.66%), and fanesyl acetate (2.30%). The highest yield of oil (43.89%) in Z. piperitum leaves was obtained in May but decreased gradually beginning in June. The yield of essential oil from Z. piperitum leaves during early periods was higher than that during later periods and usually decreased from early maturation stages to subsequent stages. However, in contrast to leaves, the oil yield in Z. piperitum fruit increased in June, and oil yield later in the season was higher than that earlier in the season. These results indicate that the essential oil produced from Z. piperitum leaves at the early developmental stages was stored in leaves, and might be transferred to fruit at the final developmental stages.

• Korean Journal of Medicinal Crop Science
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• v.7 no.2
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• pp.115-120
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• 1999

This study was carried out to compare the essential oil and its composition, and estragol content at different growth stages in the local collections of A. rugosa. Estragol was the major constituent in essential oil of all collections tested. Estragol content in essential oil was $82.3{\sim}89.1%$ in leaves, $81.1{\sim}89.2%$ in inflorescence, $72.0{\sim}45.2%$ in stem and there was no significant difference among nine local colletions. At full bloom stage, essential oil content was significantly higher than other growth stages and the highest content of estragol in essential oil was appeared at this stage. Especially, the content of essential oil in the inflorescence of Mokpo collection was 3.3%, which was the highest among the collections. Mokpo and Jindo collections produced much more essential oil and estragol than the other collections both at full bloom and ripening stages.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.2
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• pp.128-133
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• 2000

This study was carried out to investigate the effect of forms and levels of nitrogen fertilizer on plant growth and essential oil production of Agastache rugosa. Calcium nitrate had more influenced on length and width of leaves and lateral branch length than did urea. When nitrogen fertilizer level was increased from 12 kgN/I0a to 24kgN/I0a, plant growth was stimulated and dry matter of leaf and inflorescence were increased. Top dry matter of plant with calcium nitrate treatment (38.4 g) was heavier than that of urea treatment (32.8 g). Interactions among accession and nitrogen form and nitrogen rate were not significantly different for top dry matter. The forms and rate of nitrogen fertilizer did not affect estragole content. The estragole contents was higher in leaf (91.8%) than that of inflorescence (81.3%). While the essential oil content was not affected by different nitrogen forms, nitrogen level affected the essential oil contents positively by increasing dry matter. Essential oil yield was not affected by accession or nitrogen form, but by nitrogen rate. With increasing N application from 12kgN/I0a to 24 kgN/I0a, essential oil yield was increased by 95.8 %.

• Food Science and Biotechnology
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• v.14 no.3
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• pp.350-354
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• 2005

The effect of the essential oil obtained from Chrysanthemum boreale Makino on the apoptosis of KB cells was investigated. Cytotoxicity and cellular DNA content were analyzed by MTT assay, flow cytometry, agarose gel electrophoresis, and Hoechst 33258 staining. The caspase-3 and poly (ADP-ribose) polymerase (PARP) proteins were estimated by Western blotting method. The various cytotoxic effects of the essential oil which are hallmarks of apoptosis, including DNA fragmentation, apoptotic body formation, and sub-G1 DNA content, all progressed in a dose-dependent manner. Treatment with an apoptosis-inducing concentration of the essential oil caused rapid and transient induction of caspase 3 activity. Further, the efficacious induction of PARP cleavage and caspase-3 activation was observed at an essential oil concentration of 0.1 and 0.2 mg/mL for 12 hr.

• Journal of Forest and Environmental Science
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• v.30 no.3
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• pp.285-292
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• 2014

Essential oil yields and chemical compositions from 5 populations of Chamaecyparis obtusa with several environmental factors were investigated through essential oil extracted distillation apparatus and metabolite profiling by GC-MS analysis. Among the populations, content of essential oil at Gokseong was significantly higher than other populations. To compare the several environmental factors affecting on chemical composition and essential oil yields from C. obtuse at Gokseong, the environmental factors (soil condition, temperature, humidity, and moisture content) were measured during 1 year. The essential oils at Goksung based on humidity on March, July, and November was significantly different from other months. The essential oils at Goksung based on temperature on July and August was significantly different from other months. The essential oils at Goksung based on the moisture content on September were significantly different from other months. The percentage of T-N, OM, and yield of oil at Gokseong were significantly different on from other populations. The main constituents of C. obtusa at all populations were ${\alpha}$-pinene, ${\beta}$-pinene, ${\alpha}$-terpinene, ${\gamma}$-terpinene, terpinene-4-ol, isobonyl acetate, terpinyl acetate, and cedar acetate. Specially, Essential oil compositions (%) of ${\alpha}$-terpinene and cedar acetate were higher at Gokseong than at other populations. The chemical compositions of essential oils were variable depend on populations and environmental conditions. Therefore, this study might be used as fundamental research on study for selection of high productive terpenoids and for understanding about biosynthesis of essential oils in C. obtusa.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.38 no.1
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• pp.55-59
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• 1993

Essential oil components and contents were investigated at different growth stages and organs of Schizonepeta tenuifolia Briquet. The content of essential oil different by growth stages and organs and the amount of essential oil at leaf was higher than any other organ. The content of essential oil at leaf and spica were increased by long growth period. The d-menthone and l-pulegone were maj or components of essential oil and contain 51-76% of total essential oil at leaf and spica. In the early stage leaf. l-pulegone was major component of essential oil but d-menthone was major components in the late stage leaf.

• Korean Journal of Medicinal Crop Science
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• v.10 no.4
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• pp.298-302
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• 2002

The essential oils were isolated by hydrodistillation and their composition determined capillary GC method with standards. The essential oil content showed significant differences between the two populations on the vegetative organs. The essential oil level of the leaves and roots was considerably higher in the Korean population at full flowering and waxy ripening stage but essential oil content of the roots was significantly higher in the Hungarian taxon at leaf rosette stage. We observed the essential oil accumulation tendency was mianly dependent on plant organs and intra-specific taxon during the vegeation period. Butylidene-phthalide was proved to be the main component of the oil in both population roots (50.9-73.3%), while dimethyl-acetate was showed as a major compound on the over-ground parts (56.7-62.0%). The qualitative composition of the essential oil in the reproductive organs concerning the identified compounds was the same as the vegetative parts with the main component ${\alpha}-phellandrene$ (4.8-28.1%) and butylidene-phtalide (9.7-16.1%), The quantitative composition showed some changes during the ontogenesis phases. Most characteristic ones are the decreasing proportion of dimethyl-acetate (from 7.3% to 1.1%) and the appearance of ${\alpha}-pinene$ (from 0.5% to 1.5%) only after fruit setting in both population.

• The Korea Journal of Herbology
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• v.21 no.3
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• pp.83-89
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• 2006

Objectives : To compare limonene, menthone and pulegone content from different part of Schizonepeta tenuifolia Briquet. Methods : The quantitative content of essential oil was determined according to the method of Chinese Pharmacopoeia. GC analysis of essential oil was carried out using a flame ionization detector. The results obtained were confirmed by GC/MS. Results : The content of essential was high on the leaf part. The content of menthone was higher than other ingredients. When compare the content observed by harvesting time, the highest pick was shown on middle of August. Conclusion : This research can be used to modify or feedbackthe article in the Korean Pharmacopoeia.

• Plant Resources
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• v.6 no.3
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• pp.183-187
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• 2003

Two kinds of Angelica belong to Umbelliferae collected, the one is Angelica gigas that is inhabitant in Korea and the other is Angelica acutiloba that is indigenous in Japan at the field of Snyder Research and Extension Farm Rutgers University, New Jersey and was analyszed by GC and GC/MS. The composition of the essential oil of the different aerial parts of the Angelica has been studied. The oil yields obtained upon hydrodistillation were 0.18% (v/w) in Korean Angelica and 0.44% (v/w) in Japanese Angelica on dry root weight basis. By the growing stage in the Rutgers greenhouse condition, leaf and root of essential oil content a little decreased on 9 months later than 4 months later except for Angelica gigas leaf. Both of Angelica showed that amounts of essential oil content presented in order of leaf > petiole > root according to different plant part. The analysis of the essential oil from Angelica root led to the identification of 14 constituents totaling 64% in Korean Angelica and 13 constituents totaling 68% in Japanese Angelica. The major constituents of the Angelica root essential oil were ligustilide (47 %) and gamma terpi (14 %) in Korean Angelica, and alpha pinei (32 %) and nonane (25 %) in Japanese Angelica

• Horticultural Science & Technology
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• v.19 no.1
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• pp.81-86
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• 2001

This experiment was carried out to investigate watering with relation to growth, quality and yield of essential oil in sweet basil. The degree of water stress was taken as amount of watering. D1 was watered with 25mL for 2 weeks and 30mL from 4 to 5 weeks after planting in Wagner pot (1/20000a, ${\phi}24cm$). As this, 75mL and 90mL, 225mL and 270mL, and 675mL and 810mL were watered in D2, D3, and D4 treatment respectively. DFT was set up as water stress was not inflicted. The growth of basil in D3 and D4 was better than that of others, in which root activity was as much twice as that of D1. Essential oil of D1 was recorded the lowest content as 0.33%. The result of proline content, peroxidase activity, photosynthesis, stomatal conductance and stomatal resistance were proved D1 to be stressed. This treatment consequently increased the content of essential oil. In consideration of growth and essential oil content, D3 treatment was highest as 47.37mg in oil production per plant. Finally, D3 watered with 225mL for 2 weeks and 275mL from 4 to 5 weeks after planting could be selected on the purpose of both plant growth and essential oil production. Essential oil content of sweet basil was increased in response to water stress. For increase of essential oil yield, oil synthesis could be raised by giving water stress just before harvesting.