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Effects of Refeeding with a Protein-Free Diets Supplemented with Various Essential Amino Acids on the Plasma Insulin-Like Growth Factor-I Concentration in Fasting Young Chickens

  • Kita, K;Shibata, T.;Nagao, K.;Hwangbo, J.;Okumura, J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.3
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    • pp.406-409
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    • 2002
  • The effect of refeeding with various single essential amino acids on the recovery of plasma insulin-like growth factor-I (IGF-I) concentration in fasted young chickens was examined. Young chickens (29 days of age) were divided into 15 experimental groups. Chickens in one group were fed on the commercial diet ad libitum for 4 days. The remaining 56 chickens in 14 experimental groups were fasted. After 2 days of fasting, 52 chicks in 13 fasted groups were refed with one of the following experimental diets for 2 days. Eleven experimental diets were protein-free diets supplemented with one of 11 essential amino acids (Arg, Gly, His, Ileu, Leu, Met, Phe, Lys, Thr, Trp, Val). The remaining 2 experimental diets were a protein-free diet containing 11 essential amino acids and a protein-free diet not supplemented with amino acids. Birds in the remaining fasted group continued to be fasted for 2 days. Fasting for 2 days markedly reduced plasma IGF-I concentration. When fasted chickens were refed the protein-free diet containing either Gly alone or all essential amino acids, plasma IGF-I concentration was recovered to the level similar to that of fed chickens. Protein-free diet alone, however, failed to restore the reduced IGF-I concentration in plasma. Body weight loss modulated by feeding with protein-free diets supplemented with various single essential amino acids was associated with changes in plasma IGF-I concentrations. We concluded that body weight loss by feeding with a protein-free diet was lower than that of fasted chickens and that body weight loss associated with the decrease in plasma IGF-I concentration was modulated by feeding with protein-free diets containing various single essential amino acids.

Convergence research on the possibility of development of oral care products using the anti-plaque activity of natural essential oils against Streptococcus mutans (천연에센셜오일의 Streptococcus mutans에 의한 치석형성 억제 활성을 이용한 구강관리제품 개발 가능성에 대한 융합연구)

  • Kim, Minhyung;Lee, So-Young;Min, Hee-Hong
    • Journal of the Korea Convergence Society
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    • v.9 no.7
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    • pp.149-154
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    • 2018
  • This article intended to examine the anti-plaque activity of 4 essential oils of Lavender, Tea tree, Eucalyptus, Lemongrass against Streptococcus mutans. In the results of measuring the anti-plaque effect against Streptococcus mutans, Minimum anti-adhesive concentration of Lavender oil was 1.0% and that of Tea tree, Eucalyptus, lemongrass essential oils was 0.5 %. Also, it was confirmed that the essential oils have the effect of inhibiting acid generation by Streptococcus mutans. It was confirmed that pH of the concentration was lowered by the acid generation under the MAC by measuring pH of the solution after incubating Streptococcus mutans and the essential oils in the thermostatic bath varying their concentration. From these results, the essential oils, particularly, Tea tree, Eucalyptus, Lemongrass essential oils are the natural material inhibiting the plaque generation and the potential that they can be used to develop the oral care products was confirmed.

Induction Effect of Biotic and Chemical Elicitors Treatment for the Increase of Essential Oil Content from Trees (수엽(樹葉) 정유함량에 미치는 생물.화학적)

  • Kang, Ha-Young;Choi, In-Gyu;Lee, Sung-Suk
    • Korean Journal of Food Science and Technology
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    • v.34 no.1
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    • pp.8-12
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    • 2002
  • In order to artificially increase the contents of essential oils from 4 different trees by inducing with elicitors, 5 kinds of chemical elicitors and 4 kinds of biotic elicitors were selected. Before treatment, the contents of essential oils from Japanese Cypress (Chamaecyparis obtusa), Sawara cypress (Chamaecyparis pisifera), Japanese Red Pine (Pinus densiflora), and Korean Pine (Pinus koraiensis) were 2.0, 1.6, 0.4, and 0.7 percent, respectively, and the maximum content of essential oils from all species were reached in July. By most of elicitors, the essential oil content was generally increased after 6 months later, but some of elicitors did not affect the content of essential oil. Finally, the appropriate inducers for artificially increasing the essential oil were respectively selected depending on each species; Schizophyllum commune Fries for Japanese Red Pine and Japanese Cypress, hydrogen peroxide for Korean Pine, and ${\beta}-pyridone$ for Sawara cypress. Especially, hydrogen peroxide and ${\beta}-pyridone$ could be wide spread inducer for all 4 species.

Effects of Inhalation of Wood Essential Oil (Chamaecyparis obtusa) on the Change of Concentration and Mood States (편백 정유향의 흡입이 주의집중력과 기분상태의 변화에 미치는 영향)

  • Seong, Hyeon Su;Goh, Eurah;Choi, In Hwa
    • Journal of Forest and Environmental Science
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    • v.30 no.1
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    • pp.62-70
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    • 2014
  • The principle purpose of this research is to figure out whether the inhalation of Wood Essential oil (Chamaecyparis obtusa) affect not only human beings' concentration for surroundings but also change as to status of feelings. total number of subjects for this experimental are determined to be 66 (men 44 and women 22) among 85 adults who agreed to participate in this experiment after excluding 19 participants who were not able to smell scent due to flu and nasal inflammation. After both experimental group and control group being measured referring to their status of mood such as concentration, depression, vigor, fatigue, confusion, tension and anger, the experimental group is offered to get a piece of cotton that includes Wood Essential oil and they are asked to inhale it for 5 min. On the other hand, control group is offered to wait for the experimental group finishing inhaling wood Essential oil for 5 min. After 5 min, the status of mood from both groups were measured again. simultaneously, I conducted a survey targeting experimental group to see what their preferences are like regarding scent of wood essential oil. The experiment shows that the status of experimental group's concentration is improved about 0.94 point when compared to control group's. Moreover, Total mood disturbance is lower than control group's. Therefore it is proved that the inhalation of wood Essential oil alleviates overall status of mood. according to the analyses result of favor to scent for wood essential oil from experimental group, some of them who were positive got much better improvement when it comes to concentration and far more relieved on their feelings than those who weren't. Based from this result, it is definitely confirmed that there is a Phytoncide's psychophysiological effect which transcends the Placebo Effect generated by Phytoncide's positive image.

Chemical Composition and Biological Activities of Essential Oils Extracted from Korean Endemic Citrus Species

  • Baik, Jong-Seok;Kim, Sang-Suk;Lee, Jung-A;Oh, Tae-Heon;Kim, Ji-Young;Lee, Nam-Ho;Hyun, Chang-Gu
    • Journal of Microbiology and Biotechnology
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    • v.18 no.1
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    • pp.74-79
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    • 2008
  • The aim of this study was to analyze the chemical composition of 14 kinds of citrus oils and to test their biological activities. Citrus essential oils were obtained by steam distillation from immature fruits collected from Jeju Island and were analyzed using gas chromatograph (GC)-flame ionization detectors (FID) and GC-MS. Limonene (55.4% to 91.7%), myrcene (2.1% to 32.1%), ${\alpha}$-pinene (0.6% to 1.6%) and linalool (0.4% to 6.9%) were the major components in most citrus species. To evaluate in vitro antibacterial activity, all essential oils were tested against Propionibacterium acnes and Staphylococcus epidermidis. Nine out of fourteen citrus oils exhibited antibacterial activity against P. acnes, but not against S. epidermidis. The effects of the citrus oils on DPPH radical scavenging, superoxide radical anion scavenging, nitric oxide radical, and cytotoxicity were also assessed. Three essential citrus oils, Joadeung, Dongjunggyul, and Bujiwha, exhibited potent inhibitory effects on nitric oxide production. Two essential oils, Dongjunggyul and Joadeung, showed potent free radical scavenging activities in the DPPH assay. For future applications in cosmetic products, we also performed MTT assays in a human dermal fibroblast cell line. The majority of the essential oils showed no cytotoxicity. The results indicate that citrus essential oils can be useful natural agents for cosmetic application.

Comparative analysis of amino acid contents of the fruiting bodies in Ganoderma spp. (영지버섯 균주별 자실체의 아미노산함량 비교 분석)

  • Cho, Jae-Han;Noh, Hyung-Jun;Kang, Don-Ho;Lee, Jee-Young;Lee, Min-Jung;Park, Hye-Sung;Sung, Gi-Ho;Jhune, Chang-Sung
    • Journal of Mushroom
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    • v.10 no.4
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    • pp.208-215
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    • 2012
  • Ingredient content for the general composition of essential amino acids and non-essential amino acids were analyzed by the fruiting bodies of Ganoderma sp. The results obtained are as followes. The ASI 7004 showed that high concentrations than other strains in essential amino acids(His, Ile, Leu, Phe, Thr, Val) and the non-essential amino acids(Ala, Ser, Gly). In addition, The ASI 7002 showed that high concentrations than other strains in essential amino acids(Lys) and the non-essential amino acids(Asp, Glu). ASI 7022 showed that high concentrations than other strains in essential amino acids(Met) and the non-essential amino acids(Pro). In General, contents of Amino acid was higher than Phellinus.

Antimicrobial Activity of Some Essential Oils Against Microorganisms Deteriorating Fruit Juices

  • Helal G.A.;Sarhan M.M.;Shahla A.N.K. Abu;El-Khair E.K. Abou
    • Mycobiology
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    • v.34 no.4
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    • pp.219-229
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    • 2006
  • Seventeen microbial species including 10 fungal taxa, two yeasts and five bacteria, were isolated from freshly prepared orange, guava and banana juices kept in open bottles at room temperature for 7 days. Eight different essential oils, from local herbs, were tested for their antimicrobial activity against these test organisms. The essential oils of Cymbopogon citratus, Ocimum basilicum and Origanum majorana were found to be highly effective against these microorganisms. Aspergillus niger, A. flavus and Saccharomyces cerevisiae, the most prevalent microorganisms in juice, showed the highest resistance against these essential oils. GC-MS analysis showed that while e-citral, a'-myrcene, and z-citral represent the major components (75.1 %) of the essential oil of Cymbopogon citratus; bezynen,l-methyl-4-(2-propenyl), 1,8-cineole and trans-a'-bisabolene were the main components (90.6%) of Ocimum basilicum; whereas 3-cyclohexen-l-0l,4-methyl-l(l-methylethyl)-(CAS), c-terpinene and trans-caryophyllene represent the major components (65.1%) of Origanum majorana. These three essential oils were introduced into juices by two techniques namely, fumigation and direct contact. The former technique showed more fungicidal effect than the latter one against A. flavus, A. niger, and S. cerevisiae. The essential oil of Cymbopogon citratus by comparison to other test oils showed the strongest effect against these fungi with a minimum inhibitory concentration of $1.5\;{\mu}l/ml$ medium and a sublethal concentration of $1.0\;{\mu}l/ml$. The antimicrobial activity of this oil is thermostable at $121^{\circ}C$ for 30 min.

The Essential Oil of Artemisia iwayomogi Kitamura Induces Apoptosis on Human Oral Epidermoid Carcinoma Cells

  • Jeong, Mi-Ran;Cha, Jeong-Dan;Lee, Kyung-Yeol;Kil, Bong-Seop;Han, Jong-Hyun;Lee, Young-Eun
    • Food Science and Biotechnology
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    • v.16 no.4
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    • pp.531-536
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    • 2007
  • The aerial part of Artemisia iwayomogi Kitamura has traditionally been used for inflammation, infectious disease, cancer, pyretic, diuretic, liver protective effect, and choleretic purposes in Korea. We investigated that the essential oil induces apoptosis in KB cell as evidenced by Hoechst-33258 dye staining, flow cytometry (cell cycles), and DNA fragmentation for nuclear condensation and Western blotting for activation of caspases-3, -8, -9, Bax, Bcl-2, cytochrome c, and poly (ADP-ribose) polymerase (PARP) cleavage. In the present study, we found that the essential oil could induce apoptosis in KB cells, as characterized by DNA fragmentation, activation of caspase-3, -8, and -9, and PARP cleavage. The efficacious induction of apoptosis was observed as a dose-dependent. The essential oil-induced apoptotic cell death was accompanied by up-regulation of Bax and down-regulation of Bcl-2. The essential oil also caused the loss of mitochondrial membrane potential and cytochrome c release from mitochondria to cytosol. These findings indicate that mitochondrial pathways might be involved in the essential oil-induced apoptosis and enhance our understanding of the anticancer function of the essential oil in herbal medicine.

Analyses of the Volatile Flavor Composition of Essential Oils from Chrysanthemum zawadskii var. latilobum Kitamura and Aster yomena Makino (구절초와 쑥부쟁이 정유의 휘발성 향기성분 분석)

  • Choi, Hyang-Sook
    • The Korean Journal of Food And Nutrition
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    • v.31 no.3
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    • pp.378-387
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    • 2018
  • This study investigated the volatile flavor composition of essential oils from Chrysanthemum zawadskii var. latilobum Kitamura and Aster yomena Makino. The essential oils obtained by the hydrodistillation extraction method from the aerial parts of the plants were analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). One hundred (95.04%) volatile flavor compounds were identified in the essential oil from the C. zawadskii var. latilobum Kitamura. The major compounds were valencene (10.82%), ${\delta}$-cadinol (9.77%), hexadecanoic acid (8.70%), 2-methyl-4-(2,6,6-trimethylcyclohex-1-enyl) but-2-en-1-ol (3.67%), and 2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4,4]non-3-ene (3.57%). Ninety-eight (93.83%) volatile flavor compounds were identified in the essential oil from the Aster yomena Makino. The major compounds were and 3-eicosyne (13.61%), 9,10,12-octadecatrienoic acid (7.8%), ${\alpha}$-caryophyllene alcohol (6.83%), 9-octadecynoic acid (6.03%), and ${\alpha}$-caryophyllene (5.74%). Although the two plants are apparently very similar, the chemical composition of the essential oils was significantly different in quality and quantity. In the case of C. zawadskii var. latilobum Kitamura, the sesquiterpene, valencene was found to be 10.82%, but it was not identified in A. yomena Makino. ${\delta}$-Cadinol appeared higher in C. zawadskii var. latilobum Kitamura than in A. yomena Makino. A clear characteristic of A. yomena Makino essential oil is that it has a high content of caryophyllene derivatives. The ${\alpha}$-caryophyllene alcohol contained in A. yomena Makino was relatively high at 6.83%, although the compound was not identified in C. zawadskii var. latilobum Kitamura. Also ${\alpha}$-caryophyllene was shown to be higher in A. yomena Makino than in C. zawadskii var. latilobum Kitamura.

Effects of Essential Companywide Components of PL Response System on Company's PL Performance (전사적 PL 대응시스템의 핵심요인이 기업 성과에 미치는 영향)

  • Seo, Jun Hyeok;Bae, Sung Min
    • Journal of Korean Society of Industrial and Systems Engineering
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    • v.40 no.2
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    • pp.22-30
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    • 2017
  • PL (Product Liability) refers to the legal responsibility of a manufacturer or seller for bodily injuries or property losses caused by product defects. Therefore, it is important for companies to construct a product liability response system that strategically manage and effectively adapt to product liability. A PL response system refers to companywide operations of PL prevention (PLP) measures, product safety (PS) measures, and PL defense (PLD) measures appropriate for a company's scale and environment. To establish an enterprise product liability response system, each essential component of corporations should be systematically operated and maintained considering the scale and characteristics of the corporations. Essential components of PL response system is Strategy, Organization, Training, Technology, Investment, and Awareness. Role of essential components is that companies need specific strategies to secure product safety and protect customers from product defects, and appropriate organizations must be composed for effective operation of such strategies. The objective of this paper seeks to examine the relationships among the essential components of the product liability response system and PL performance. PL performance consists of positive performance and negative performance. In particular, positive performance include increased efforts in product or process innovation such as strengthening research and development (R&D) to produce safer products without defects. In order to carry out this research we obtained 98 questionnaire of manufacturing company. A summary of the analyses is as follows: First, the awareness and technology among essential components affect significantly to the positive performance. Second, the awareness and strategy among essential components negative affect to the negative performance.