• Journal of Life Science
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• v.13 no.6
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• pp.879-888
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• 2003

In order to measure the enzyme activity of 5-epi-aristolochene hydroxylase, one of cytochrome P450 (P450) enzymes in eicitor-treated pepper cell, we used in vivo assay method and demonstrated a dramatic suppression of the activity by P450-inhibitors, ancymidol and ketocornazole. Using RT-PCR method with degenerate primer of the well conserved domains found within most P450-enzymes, and using cDNA library screening method, one distinct cDNA, being designated P450Hy01, was successfully isolated from elicitor-treated pepper cells. P450Hy01 mRNA was all induced in elicitor-treated cells whereas never induced in control cells. Moreover, levels of P450Hy01 expression were highly correlated with the levels of extracellular capsidiol production by different elicitors in cell cultures. P450Hy01 transcript was also induced by several other elicitors such as, cellulase, arachidonic acid, jasmonic acid, yeast extract as well as UV stress. P450Hy01 sequence contained high probability amino acid matches to known Plant P450 genes and ORF with a conserved FxxGxRxCxG heme-binding domain. P450Hy01 cDNA showed 98% of homology in sequence of nucleotide as well as amino acid to 5-epi-aristolochene-1, 3-hydroxylase (5EAl, 3H) which has been isolated in tobacco cells, suggesting that P450Hy01 is prominent candidate gene for P450-enzyme encoding 5EAl, 3H in pepper cell.

• Korean Journal of Microbiology
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• v.42 no.3
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• pp.210-215
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• 2006

Diplodia gossypina ATCC10936 produced chiral specific (+)-jasmonic acid (JA) that is the most biologically active form. (+)-JA is a plant growth hormone and also one of the most important aroma compounds responsible for jasmin-like aroma note. In order to develop a commercial bioprocess for the production of (+)-JA, optimal culture conditions for D. gossypina ATCC10936 were investigated. D. gossypina produced (+)-JA using either fructose and glucose as a sole carbon source. As a nitrogen source, $NaNO_3$ gave relatively high (+)-JA production. The optimal temperature for the production of (+)-JA by D. gossypina was $28^{\circ}C$, and optimal agitation was found to be 200 rpm. D. gossypina produced (+)-JA upto 600 mg/L in SM medium, although the highest level of biomass was obtained in PDMYS medium.