• 제목/요약/키워드: enzyme hydrolysate

검색결과 249건 처리시간 0.03초

바지락 단백질 Thermolysin 가수분해물의 Angiotensin Converting Enzyme 저해 Peptide의 특성 (Characteristics of Angiotensin Converting Enzyme Inhibitory Peptides from Thermolysin Hydrolysate of Manila clam, Ruditapes philippinarum Proteins)

  • 이태기;염동민;김선봉
    • 한국수산과학회지
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    • 제35권5호
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    • pp.529-533
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    • 2002
  • 바지락 단백질의 thermolysin 가수분해물로부터 분자량 한계범위가 10,000 da인 한외여과막을 통과한 저분자량의 물질을 Sephadex LH-20 column을 이용한 겔 크로마토그래피에서 ACE에 대하여 저해 활성을 가지는 3개의 획분을 분취하여 SP-Toyopearl 650S column과 SuperQ-Toyopearl 650S column을 이용한 이온 교환 크로마토그래피에 의하여 4개의 활성 획분을 얻었다. 이 중 가장 높은 절해 활성을 나타내는 획분의 아미노산 조성은 alanine, leucine, isoleucine 및 threonine의 함량이 많았고, 활성 발현에 있어 C 말단 아미노산 잔기로서 중요한 역할을 하는 proline의 함량도 $3.7\%$%인 것으로 나타났다. ACE 저해 활성은 $IC_{50}$ 값이 0.748 $\mu$g이었다.

팔당호에서 종속영양 활성도의 계절적 변화 및 세균의 세포외 효소활성 (Seasonal Fluctuations of Heterotrophic Activity and Bacterial Extracellular Enzyme Activity in Paldang Lake)

  • 김상진
    • 미생물학회지
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    • 제31권1호
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    • pp.93-98
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    • 1993
  • 수계생태계에서 유기물질의 순환을 이해하기 위하여 팔당호에서 종속영양 활성도와 세균세포의 효소활성의 계절절 변화를 연구하였다. 팔당호 I 의 glucose 전환시간은 수층, 퇴적토에서 2-1,300 시간, 17-170 시간, protein hydrolysate 는 5-900 시간, 15-240 시간, acetic acid 는 4-350 시간, 15-230 시간으로 계절적인 변화를 나타냈다. Glucose, protein hydrolysate, acetate 각각의 호흡율은 수층에서 23-32%, 38-41%, 22-28%로 나타났고 퇴적토에서는 34%, 61% and 41% 로 나타났다. 이 결과로 3가지 유기물질 종류 모두가 수층보다 퇴적토에서는 높은 율로 호흡됨을 알 수 있었다. 한편 세균의 $\alpha$-glucosidase, $\beta$-glucosidase, N-acetyl-$\beta$-D-glucosaminidase, aminopeptidase 활성력을 살펴본 결과 수층에서는 효소 각각에 대해 32-44%, 31-32%, 18-34% 61-67% 의 범위를 나타내었고 퇴적토에서는 34%, 40%, 23% 65%로 나타났다.

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Anti-adipogenic Effect of Hydrolysate Silk Fibroin in 3T3-L1 Cells

  • Chon, Jeong-Woo;Lee, Kwang-Gill;Park, Yoo-Kyoung;Park, Kyung-Ho;Yeo, Joo-Hong
    • International Journal of Industrial Entomology and Biomaterials
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    • 제21권2호
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    • pp.169-174
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    • 2010
  • Hydrolysate silk fibroin (HSF) is a fibrous protein composed of parallel $\beta$-structures and is made from pure silk elements including 18 amino acids, with glycine, alanine, and serine comprising of over 80% of the amino acids. Numerous studies have documented a range of effects of HSF, including moisturizing, antioxidant activity, nervous system disorders, and many more. We investigated whether HSF has anti-obesity effects in vitro. The effects of HSF inhibition on lipid accumulation and acceleration of lipid degradation in 3T3-L1 cells were studied. Treatment of 3T3-L1 cells with HSF caused significant inhibition of cell viability, an increase in glycerol release, and a decreased in adipocyte differentiation. Moreover HSF stimulated downregulated of adipogenic enzyme expressions (PPAR${\gamma}$ and C/EBP${\alpha}$) and up-regulated of fatty oxidation enzyme expressions (CPT-1 and UCP-2). Based on these results, hydrolysate silk fibroin can be suggested as a potential therapeutic substance as part of a prevention or treatment strategy for obesity.

Angiotensin I-Converting Enzyme (ACE) Inhibitory Activity of Elk (Cervus elaphus) Velvet Antler

  • Karawita Rohan;Park, Pyo-Jam;Siriwardhana Nalin;Jeon, Byong-Tae;Moon, Sang-Ho;Ahn, Duk-Kyun;Chos, Somi-K.;Jeon, You-Jin
    • Preventive Nutrition and Food Science
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    • 제10권3호
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    • pp.239-243
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    • 2005
  • Angiotensin I-converting enzyme (ACE) inhibitory activities of elk antler hydrolysates prepared with three kinds of proteases, pepsin, trypsin and $\alpha-chymotrypsin$, were investigated. The ACE inhibitory activity of the pepsinolytic hydrolysate was the highest with an $IC_{50}$ value of $9.3\mu g/mL.$ In addition, three kinds of pepsinolytic hydrolysates with relatively high molecular weights (over 10,000 Da), medium molecular weights (5,000 to 10,000 Da), and low molecular weights (below 5,000 Da) were fractionated using an ultrafiltration membrane system. The below 5,000 Da hydrolysate exhibited the highest ACE inhibitory activity. These results indicate that the pepsinolytic hydrolysates of elk velvet antler could be a good source of peptides with ACE inhibitory activity.

Memory-Enhancing Effects of Silk Fibroin-Derived Peptides in Scopolamine-Treated Mice

  • Kang, Yong Koo;Lee, Woojoo;Kang, Byunghoon;Kang, Hannah
    • Journal of Microbiology and Biotechnology
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    • 제23권12호
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    • pp.1779-1784
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    • 2013
  • Although enzyme-hydrolyzed silk fibroin has been reported to enhance cognitive function before, it has been still unknown which peptides can improve memory. Here we report that amino acid sequences of three novel peptides were identified from fibroin hydrolysate. Fibroin hydrolysate was obtained by hydrolysis with protease after partial hydrolysis with 5M $CaCl_2$. Synthesized peptides derived from these sequences improved scopolamine-induced memory impairments in mice. We confirmed this hydrolysate had effects that improved learning and memory abilities by performing the Rey-Kim test. From this hydrolysate of silk fibroin, amino acid sequences of eight peptides were identified by LC-MS/MS. Three peptides (GAGAGTGSSGFGPY, GAGAGSGAGSGAGAGSGAGAGY, and SGAGSGAGAGSGAGAGSGA) were synthesized to investigate whether they could improve memory. Passive avoidance test and Morris water maze test were performed, and all peptides showed memory-enhancing abilities on scopolamine-induced memory impairments in mice. In this study, we identified three novel peptides that could improve memory, and that silk fibroin hydrolysate was a mixture of various active peptides that could enhance memory.

3단계 막효소반응기에서 연속적으로 생산된 어피 및 우피 젤라틴 가수분해물의 항산화활성 비교 (Comparison of Antioxidative Activity on Fish and Bovine Skin Gelatin Hydrolysates Produced in a Three-Step Membrane Enzyme Reactor)

  • 김세권;박표잠;송병권;김종배
    • KSBB Journal
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    • 제15권6호
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    • pp.635-643
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    • 2000
  • To compare the antioxidative activities of fish skin and bovine skin gelatin hydrolysate, gelatin hydrolysates from Alaska pollack and bovine skin were prepared by various enzymatic hydrolysis methods (1st step, Alcalase; 2nd step, pronase E; 3rd step, collagenase) using a continuous three-step membrane reactor. The molecular weight distributions of the 1st, 2nd and 3rd step hydrolysates were 7∼10 kDa, 2∼5 kDa and 0.7∼0.9 kDa, respectively. The antioxidative activity of fish skin gelatin hydrolysate was stronger than that of bovine skin gelatin hydrolysate, and in particular, both of 2nd step hydrolysates showed more antioxidative activity than hydrolysates of any other step. The optimum antioxidative activity concentration of the 2nd step hydeolysates of fish and boving skin were 1% (w/w) in a linoleic acid water-alcohol emulsion. In cultured cells exposed to t-butyl hydroperoxide (t-BHP), the 2nd step hydrolysate of fish skin gelatin delayed cell death most. These results suggest that the antioxidative activity of fish skin gelatin hydrolysate is higher than that of bovine skin gelatin hydrolysate because of their different amino acid contents.

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Angiotensin I Converting Enzyme Inhibitory Activity of Krill (Euphausia superba) Hydrolysate

  • Kim Dong-Soo;Park Douck-Choun;Do Jeong-Ryong
    • Fisheries and Aquatic Sciences
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    • 제5권1호
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    • pp.21-27
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    • 2002
  • Angiotensin I converting enzyme inhibitory activities of shelled krill (Euphausia superba) hydrolysates by autolysis and by hydrolysis with commercial proteases were analyzed. Among the proteases, Alcalase was the most effective protease for the hydrolysis of krill considering the degree of hydrolysis $(87.5\%)$ and the ACE inhibitory activity $(60\%)$. Four hour hydrolysis suggested as the most suitable and economic. In order to establish the optimum hydrolysis condition of krill, degree of hydrolysis and ACE inhibitory activity as affected by Alcalase concentration and water amount added were statistically analyzed by response surface methodology (RSM). The optimum hydrolysis condition was $2.0\%$ Alcalase hydrolysis in 2 volumes (v/w) of water at $55\% for 4 hr. The hydrolysate prepared from the optimum hydrolysis condition was fractionated by molecular weight. The lower molecular weight fraction showed the higher ACE inhibitory activity. $IC_{50}$ of the fraction under 500 Da was 0.57mg protein/mL.

창오징어 2단 효소분해엑스분의 정미특성 및 기능성 (Taste Characteristics and Functionality of Two Stage Enzyme Hydrolysate from Low-Utilized Longfinned Squid)

  • 오광수
    • 한국식품영양과학회지
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    • 제30권5호
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    • pp.782-786
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    • 2001
  • 본 연구는 수산가공용 풍미소재 검색 및 인스탄트식품용 조미소재의 개발, 연안에서 생산되는 저활용 수산자원의 유효이용이라는 관점에서 소형 창오징어를 원료로 열수추출엑스분, 자가소화엑스분 및 2단 효소분해엑스분의 조제하여 이들의 정미특성과 기능성을 분석.비교하였다. 소형 창오징어 열수추출 및 자가소화엑스분, 2단 효소분해엑스분의 유리아미노산 총량은 각각 2,792.5 mg%, 8,393.8 mg% 및 9,186.1 mg%이었고, 2단 효소분해엑스분에는 Asn, Gln, val, Ile 및 Lys이 다량 함유되어 있었다. ATP관련물질 중 AMP 함량은 34.6~51.6 mg% 함유되어 있었고, 추출에 따른 함량 차이는 크지 않았다. 무기이온 성분으로서 Na, Cl 및 PO$_4$의 함량이 많았고, 열수추출에 비해 효소분해엑스분 쪽의 함량이 훨씬 많았다. ACE 저해능의 경우, 열수추출엑스분이 10.1%, 자가소화엑스분은 80.2%, 1차효소분해엑스분은 87.5%, 2차 효소분해엑스분은 92.1%로, 효소분해엑스분들이 월등히 높은 ACE 저해능을 나타내었다.

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도루묵 가수분해물 유래 항염증 펩타이드 제조를 위한 효소 가수분해 최적 조건 (Optimal conditions of enzymatic hydrolysis for producing anti-inflammatory peptides from sandfish (Arctoscopus japonicus) hydrolysate)

  • 장혜림;윤경영
    • 한국식품과학회지
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    • 제50권2호
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    • pp.203-208
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    • 2018
  • 본 연구에서는 항염증 펩타이드를 생산하기 위해 도루묵(Arctoscopus japonicus)의 어육과 알로부터 가수분해물을 제조하였으며, 단백분해효소, pH, 온도, 효소 농도 및 가수분해 시간에 따른 NO 소거활성을 측정함으로써 최적 가수분해 조건을 설정하였다. 어육 가수분해물의 최적 조건은 pH 5.0, 온도 $30^{\circ}C$, 효소 농도 1%, 가수분해 시간 4시간으로 확인되었으며, 알 가수분해물의 최적 조건은 pH 5.0, 온도 $70^{\circ}C$, 효소 농도 3%, 가수분해 시간 3시간으로 확인되었다. 이러한 최적의 가수분해 조건에서 어육 및 알 가수분해물의 NO 소거활성은 각각 18.94 및 19.81%로 측정되었다. 이는 도루묵 단백질 가수분해물 유래 항염증 펩타이드를 생산하기 위한 기초자료로 활용할 수 있을 것으로 기대된다.

명태단백 Pronase 가수분해물의 제조 (Preparation of Pronase Hydrolysate from Alaska-pollack)

  • 서형주
    • 한국식품영양학회지
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    • 제8권4호
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    • pp.335-343
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    • 1995
  • In order to enhance the utility of alaska-pollack, the optimum conditions for the preparation of pronase hydrolysate. The optimum temperature and pH for the hydrolysis of alaska-pollack by pronase were 4$0^{\circ}C$ and pH 7.0. The reaction time and enzyme concentration were 4 hr and 1,000 units per g of substrate. Under the above optimum conditions alaska-pollack was hydrolysed by pronase yielding a hydrolytic degree of about 89eye. The bitterness and hyrophobicity of pronase hydrolysate were decreased with increasing reaction time. Hydrophobic amino acids(Tyr, Met, Ala, flu, Leu, and Phe) were increased for 2 hr, but fur thor hydrolysis was showed decrease of hydrophobic amino acids content. Palatable amino acids (Asp, Glu, Pro, Ser, Thr and Gly) were increased with hydrolysis time.

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