• Preventive Nutrition and Food Science
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• 제21권4호
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• pp.338-347
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• 2016

The present study investigated the volatile compound, physicochemical, and antioxidant properties of beany flavor-removed soy protein isolate (SPI) hydrolyzates produced by combined high temperature pre-treatment and enzymatic hydrolysis. Without remarkable changes in amino acid composition, reductions of residual lipoxygenase activity and beany flavor-causing volatile compounds such as hexanol, hexanal, and pentanol in SPI were observed after combined heating and enzymatic treatments. The degree of hydrolysis, emulsion capacity and stability, 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity, and superoxide radical scavenging activity of SPI were significantly increased, but the magnitudes of apparent viscosity, consistency index, and dynamic moduli (G', G") of SPI were significantly decreased after the combined heating and enzymatic treatments. Based on these results, it was suggested that the enzymatic hydrolysis in combination with high temperature pre-treatment may allow for the production of beany flavor-removed SPI hydrolyzates with superior emulsifying and antioxidant functionalities.

• 약학회지
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• 제5권1호
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• pp.45-50
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• 1960

Some enzymatic properties of Cynthia roretzi v. (Drasche Korean : U-Rung-Shei) was studied by author and obtained the following results; 1. The optimum pH of the digestive gland amylase was 6.8-7.0 2. Activity of metallic ion on the amylase showed the following order; 10$^{-3}$ M M $n^{++}$>10$^{-3}$ M $Co^{++}$>10$^{-4}$ M $Mg^{++}$>10$^{-4}$ M $Ca^{++}$>10$^{-2}$ M Z $n^{++}$>10$^{-2}$ M P $b^{++}$ 3. The digestive gland enzyme inactivated at 70.deg. C. 4. When the enzyme concentration increase 2 times, the enzymatic activity also increase, but not propertionally. 5. The digestine gland amylase showed remarkably higher enzymatic activity than the intestinal amylase. 6. The digestive gland amylase from the ascidian showed remarkably higher enzymatic activity than the heptancreatic amylase from shell fish (Turbo (Batillus) Cornutus Solander).nder).nder).

• Journal of Forest and Environmental Science
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• 제33권2호
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• pp.154-159
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• 2017

Enzymatic treatment was conducted to hydrolyze pure cellulose nanofiber (PCNF), holocellulose nanofiber (HCNF), and lignocellulose nanofiber (LCNF) for 6, 24 and 72 hours and thus-obtained nanofibers (1, 3, 5, 10 wt%) were used to reinforce polyvinyl alcohol (PVA). Glucose production yield was increased by enzymatic hydrolysis. Tensile strength and elastic modulus of all PVA nanocomposite reinforced three nanofibers were improved by increasing enzymatic hydrolysis time of nanofibers and these values were higher in order of nanocomposite reinforced with PCNF>HCNF>LCNF. Furthermore, tensile properties of nanocomposite with PCNF were increased by nanofiber content. Thermal stability of PVA was improved by adding nanofibers and by increasing nanofiber content.

• Journal of Nutrition and Health
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• 제14권3호
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• pp.146-150
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• 1981

미강으로부터 추출한 lipase의 몇가지 효소 화학적 성질을 알아 보았다. 반응시간에 따른 유리 지방산의 생성율은 $35^{\circ}C$에서 30분후에 가장 높았다. 효소의 활성화 에너지는 1.83kca1/mo1e이였으며, olive oil emulsion을 기질로 사용하였을때 Km값은 $0.8{\times}10^{-3}mol/{\ell}$였다. 효소 활성은 Ca++이온에 의해 보다 촉진되었다. 미강의 효율적 이용 및 찰의 저장에 대한 이들 효소화학적 성질의 응용성이 토의 되었다.

• 펄프종이기술
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• 제37권1호
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• pp.47-52
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• 2005

Enzymatic pulping properties of mixed office waste paper in standard disintegrator were investigated for successful enzymatic deinking of mixed office waste paper. Enzymatic pulping need more revolution in standard disintegrator than alkaline pulping and Cellusoft need more revolution than Denimax. The freeness of disintegrated pulp with enzyme was higher than those of disintegrated pulps with alkaline and heat killed enzyme. The freeness of disintegrated pulp with Denimax was higher than that of disintegrated pulp with Cellusoft. The freeness of disintegrated pulps were increased with a dosage of enzymes. The mechanical properties of disintegrated pulp were improved with enzyme addition comparing with heat killed enzyme. The tensile and burst index of hand sheet of disintegrated pulps with acidic Cellusoft were higher than that of others.

• 한국식품저장유통학회지
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• 제7권2호
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• pp.184-188
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• 2000

This study was conducted to investigate enzymatic maceration of radish, cabbage, soybean sprout and carrot with cell separating enzymes in order to obtain individual cells. We studied the macerating properties of Macerozyme R-200, Macerozyme R-10 and Sumyzyme MC on vegetables. Degree of enzymatic maceration was decreasing order of radish, carrot, cabbage and soybean sprout. The degree of enzymatic maceration among macerating enzymes was decreasing order of Macerozyme R-200, Macerozyme R-10 and Sumyzyme MC. The degrees of enzymatic maceration of radish, carrot, soybean sprout and cabbage treated with Macerozyme R-200 for 2hr were 91.0, 80.6, 62.5 and 35.1%, respectively. Total pectin, color and viscosity of carrot macerates increased as the maceration rate increased.

• 동아시아식생활학회지
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• 제4권3호
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• pp.87-96
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• 1994

This study was carried out to investigate the effect of hydrolysis by proteolytic enzymes on the functional properties of sesame protein concentrate. Sesame protein concentrate was hydrolyzed with papain, pepsin and trypsin to obtain 10% and 20% degree of hydrolysis. The nirogen solubility in water was increased with increasing the degree of hydrolysis. Bulk density was increased by enzymatic hydrolysis but water absorption capacity was increased only in the case of pepsin-hydrolyzed SPC. Higher fat absorption capacity was found in SPC with 10% DH than SPC with 20% DH. Emulsifying activity was also increased by enzymatic hydrolysis except SPC with 10% DH by papain.

• 펄프종이기술
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• 제33권1호
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• pp.30-37
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• 2001

The effects of enzymatic treatment of recycled fiber were investigated to obtain the basic informations which can be used to improve the papermaking properties of recycled fiber. The recycled fibers were prepared by the repeated handsheet making and disintegrating of hardwood of hardwood and softwood kraft pulp. Novozym 342, Dinimax and Pulpzyme HC were used for enzymatic treatment. The change of fiber length distribution, freeness, contact angle and crystallinity of pulp were measured. The brightness, opacity, breaking and tear index of paper were also measured. The enzymatic treatment decreased long fiber fraction of recycled hardwood fiber, but increased long fiber fraction of recycled softwood fiber. Freeness was decreased with 0.1% enzyme and then increased again with the increase of th enzyme dosage. The improvement of flexibility of recycled fiber was obtained through the decrease of contact angle that is resulted from the decrease of crystallinity of fiber. Brightness and opacity were affected by the type of pulp and enzyme, and dosage of enzyme. Breaking length of recycled hardwood fiber was improved with enzyme treatment, but breaking length of recycled softwood fiber was decreased except for 0.01% Pulpzyme treatment. Tear index was decreased with enzymatic treatment and the lowest decrease was observed with the treatment to Pulpzyme.

• BMB Reports
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• 제32권6호
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• pp.599-604
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• 1999

A highly conserved amino acid, glutamic acid (Glu), present at position 152 in the catalytic domain of the human immunodeficiency virus type 1 (HIV-1) integrase (IN) protein has been known to be critical for enzymatic function since substitution of Glu 152 with other residues results in a complete loss of enzymatic activities. In order to better understand the role of Glu 152 as a conserved residue in enzymatic action, intragenic second site mutations have been introduced around residue 152 of a mutant IN (E152A), and their biochemical properties were analyzed in terms of enzymatic activities. Disintegration activities were found to be significantly restored in several second site mutant INs, while integration activities were only recovered weakly. However, endonucleolytic activities were not discovered in all the mutant INs. These findings indicate that the second site mutations can partially restore that catalytic structure of the active site disturbed by the E152A mutation and lead to the regaining of integration and disintegration activities. In addition, it is also suggested that endonucleolytic activity requires a more accurate structure of the catalytic site than that for the integration and disintegration activities.

• 한국식생활문화학회지
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• 제12권4호
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• pp.375-382
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• 1997

The establishment of optimal producing condition for rice-based infant foods using modified rice powder was attempted. The modification of rice powder was prepared by microwave heating as well as partial enzymatic hydrolysis. Not only thermal treatment but also enzymatic hydrolysis on rice powder increased D.E. value from 1.25 to 3.81. The water binding capacity, swelling power, solubility, digestibility by ${\alpha}-amylase$, light transparency, and paste clarity of the modified rice powder compared to unmodified rice powder were increased from 107% to 249%, from 7.80 to 42.52, from 0.04% to 0.89ft, from 9.19% to 23.01, from 33% to 42%, and from 2.2% to 3.9%, respectively. On the other hand, gelatinization temperature, apparent viscosity, and degree of retrogradation of modified rice powder showed negative correlation with D.E. value. The 「results suggested that the thermal and enzymatic treatment on rice powder improved the physicochemical properties of rice based infant food by enhancing carbohydrate absorptionability and lowering the viscosity and opacity.