• 제목/요약/키워드: enzymatic hydrolysis rate

검색결과 95건 처리시간 0.031초

대체연료(代替燃料) 생산(生産)을 위한 목질재료(木質材料)의 가수분해(加水分解)에 관한 연구(硏究)(II) - 탈(脫)리그닌처리가 폭쇄처리재(爆碎處理材)의 효소적(酵素的) 당화(糖化)에 미치는 영향(影響) - (Studies on the Enzymatic Hydrolysis of Lignocellulosic Materials for the Alternative Fuels (II) - The Effect of Delignification Treatment on the Enzymatic Hydrolysis of Steam - Exploded Woods -)

  • 조남석
    • Journal of the Korean Wood Science and Technology
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    • 제18권4호
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    • pp.18-25
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    • 1990
  • As polysaccharides in lignocellulosic materials are encrusted with aromatic lignin molecules and have high crystallinity, these require pretreatment to improve their digestability by cellulolytic enzymes. Though a number of pretreatment methods have been proposed, the steam explosion process is evaluated as a promising method. This study was performed to investigate the effect of delignification treatment by alkali, methanol and the others on the enzymatic hydrolysis. Delignification treatment resulted in great increase rate in enzymatic hydrolysis. Concerning to the effect of delignication reagents on the enzymatic hydrolysis, methanol treatment was more effective than alkali in the case of oak wood. In pine wood, the delignification did not showed any significant enhancement of hydrolysis rate. Complete delignification by Alkali-Oxygen. Alkali treatment showed high saccharification rate of 99.5%.

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목질 재료의 자기가수분해 및 효소당화에 관한 연구 (Ⅲ) - Cellulase 효소의 회수 및 재사용 - (Autohydrolysis and Enzymatic Saccharification of Lignocellulosic Materials(III) - Recycling and Reutilization of Cellulase Enzyme -)

  • 조남석
    • Journal of the Korean Wood Science and Technology
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    • 제17권3호
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    • pp.45-51
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    • 1989
  • A major problem in the enzymatic hydrolysis of lignocellulosic substrates is the very strong bonding of cellulase to lignin and even cellulose in the hydrolysis residues. This phenomenon inhibits recycle of the cellulase which is a major expense of the enzymatic hydrolysis process. In this paper, autohydrolyzed wood was delignified by two-stage with a 0.3% Na OH extraction and oxygen-alkali bleaching and was subjected to enzymatic hydrolysis with cellulase. Also, an improved almost quantitative recycle process of cellulase enzyme was discussed. In enzyme recovery by affinity method. the first recycling showed relatively high hydrolysis rate of 97.4%. Even at the third recycle. hydrolysis rate was 86.7 percents. In the case of cellulase recovery by ultrafiltration method, first 2 recycling treatments resulted very high hydrolysis rate(97.0-97.7%). Even the third recycling showed about 94.2%. Authoydrolysis of oak wood followed by 2-stage delignification with alkali and oxygen-alkali produced a substrate for enzymatic hydrolysis that allowed almost quantitative recycle of cellulase.

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Optimization of organosolv pretreatment with sulfuric acid for enhancing enzymatic hydrolysis of Pitch Pine (Pinus rigida)

  • 박나현;김혜연;곽기섭;구본욱;어환명;최인규
    • 한국신재생에너지학회:학술대회논문집
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    • 한국신재생에너지학회 2009년도 추계학술대회 논문집
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    • pp.505-505
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    • 2009
  • The object is to optimize the best condition of organosolv pretreatment process with sulfuric acid as a catalyst. As a material, Pitch pine (Pinus rigida) was ground and sieved through 40-mesh screen, and Celluclast and $\beta$-glucosidase were used as enzymes for enzymatic hydrolysis. Pretreatment processes were carried out in the minibomb, and 20 g of materials with 200 ml of 50% ethanol solution (v/v) with 1% sulfuric acid as a catalyst. Pretreatment temperature was varied from $150^{\circ}C$ to $190^{\circ}C$, and time was varied from 0 to 20 min. Then, residual materials were used for enzymatic hydrolysis. The best conditions were selected by estimating followed enzymatic hydrolysis rate and degradable rates after pretreatment process. The highest value of enzymatic hydrolysis rate was obtained as 55 - 60% at 160 and at $180^{\circ}C$, but the value decreased under more severe conditions. As the residual rates decreased under severe conditions, it infered that the decrease of sugar contents limits enzymatic hydrolysis rates. Combined with enzymatic hydrolysis rate, degradable rates and H-factors, the temperatures at $160^{\circ}C$ for 20 min and at $180^{\circ}C$ for 0 min were concluded as the optimized conditions where have the lowest H-factor value for considering energy input.

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대체연료(代替燃料) 생산(生産)을 위한 목질재료(木質材料)의 가수분해(加水分解)에 관한 연구(硏究) (III) - 폭쇄(爆碎)처리재의 산소분해시(酸素分解時) Cellulase 산소(酸素)의 정량적(定量的) 회수(回收)에 관하여 - (Studies on the Enzymatic Hydrolysis of Lignocellulosic Materials for the Alternative Fuels(III) - Quantitative Recycling of Cellulase Enzyme in the Enzymatic Hydrolysis of Steam-Exploded Woods -)

  • 조남석;임창숙;이재성;박신
    • Journal of the Korean Wood Science and Technology
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    • 제19권1호
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    • pp.14-21
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    • 1991
  • Steam-exploded woods were delignified by two-stage with a 0.3% NaOH extraction and oxygen-alkali bleaching and were subjected to the enzymatic hydrolysis with cellulase enzyme. Also, an improved almost quantitative recycle process of cellulase enzyme was discussed. In enzyme recovery by affinity method, The first recycling showed relatively high hydrolysis rate of 96.4%. Even at the third recycle, hydrolysis rate was 87.0 percents. In the case of cellulase recovery by ultrafiltration method, first 2 recycling treatments resulted in very high hydrolysis rates, 96.8% and 95.0%, respectively. Even the third recycling showed about 93.6%. Steam-explosion treatment of oak wood followed by 2-stage delignification with alkali and oxygen-alkali produced a excellant substrate for the enzymatic hydrolysis that allowed almost quantitative recycle of cellulase.

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도시 폐기물로부터 알코올 생산 (II) - 물리적, 화학적 전처리된 폐지의 효소가수분해 조건 검토 - (The Production of Alcohol from Municipal Waste(II) - The Effects of Physical or Chemical Treatment on the Enzymatic Hydrolysis of Waste Paper -)

  • 임부국;양재경;이종윤
    • Journal of the Korean Wood Science and Technology
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    • 제25권1호
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    • pp.65-70
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    • 1997
  • The effects on the enzymatic hydrolysis of waste paper treated with physical or chemical treatment were investigated. To gain the higher saccharification rate, physical or chemical treatment are necessary in enzymatic conversion process of waste paper. The major deterrents to the effective utilization of waste paper for enzymatic conversion process are phenolic compounds, cellulose crystallinity and coating materials. In the enzymatic hydrolysis of waste paper, the deterrents through enzymatic conversion process can be eliminated by the physical or chemical treatment. This study was performed to obtain the optimal condition for enzymatic conversion process of non-treated waste paper and to review effects on enzymatic conversion process of waste paper treated with physical or chemical methods. In the aspect of saccharification rate, waste paper treated with 1.5% sodium hypochlorite was the most effective and in physical treatment methods, multi-stage treatment(autohydrolysis+refining treatment) was more effective than the other physical treatment.

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오존 처리에 의한 목재 세포벽의 미세구조변화와 효소가수분해 (Enzymatic hydrolysis and micro-structure of ozone treated wood meal)

  • 김강재;엄태진
    • 펄프종이기술
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    • 제42권3호
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    • pp.67-73
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    • 2010
  • Pine (Pinus densiflora) and aspen (Populus euramericana) wood meals were treated with ozone at various time schedule in acidic condition. The lignin contents and surface area of the ozone treated wood meals were determined and the enzymatic hydrolysis rate of ozonated wood meals was evaluated. The feasibility of enzymatic hydrolysis of the ozone treated wood meal was obviously influenced with the degree of delignification. After ozone treatment of wood meal for 10min, total pore volume were slightly increased in the surface of wood meal. When wood meals were treated with ozone longer than 10min, few change in the pore volume was observed. However, the area of over $50{\AA}$ of pore size is increased with ozonation time. As a conclusion, the rate of enzymatic hydrolysis of wood is more effective with the pore size distribution than the total pore volume.

전처리 공정에 따른 폐 신문지의 효소 가수분해 특성

  • 문남규;이재환;김성배
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2000년도 춘계학술발표대회
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    • pp.459-462
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    • 2000
  • The pretreatment of used newspaper for the enzymatic digestion preprocess was performed on a percolation reactor and a batch reactor. The test condition of percolation process was $170^{circ}C$, 60min, 1 mL/min, and 400psi, that of batch was $40^{circ}C$, 3hr. and latm Reaction solutions used in pretreatment process were aqueous ammonia, sulfuric acid, water, and hydrogen-peroxide as an oxidizing agent. As a result, the effect of pretreatment was similar to batch and percolation process, but the yield of enzymatic hydrolysis was higher in batch than percolation. This batch pretreatment enhanced enzymatic hydrolysis rate and increased glucose yield from about 15 to 20%. The inhibition factors influenced the rate of enzymatic hydrolysis was investigated, and the ink contented newspaper was the major factor.

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Enzymatic Hydrolysis of Egg Yolk Protein in Continuous Packed Column Operation

  • Kang, Byung-Chul;Lee, Sang-Uk
    • 한국생물공학회:학술대회논문집
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    • 한국생물공학회 2003년도 생물공학의 동향(XIII)
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    • pp.485-489
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    • 2003
  • Enzymatic hydrolysis of egg yolk Protein was carried out in continuous packed column reactor Five supports for enzyme immobilization were evaluated in this study. We investigated the optimum operation variables - pH, temperature, and flow rate in continuous reactor operation.

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바이오에탄올 생산을 위한 암모니아수에 의해 전처리된 볏짚의 효소당화 특성 (Enzymatic Hydrolysis Characteristics of Pretreated Rice Straw By Aqueous Ammonia for Bioethanol Production)

  • 박용철;김준석
    • Korean Chemical Engineering Research
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    • 제49권4호
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    • pp.470-474
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    • 2011
  • 볏짚은 한국에서 매년 대량 생산되는 주요 작물이다. 침지공정을 이용한 목질계 바이오매스의 전처리는 대기압과 $60^{\circ}C$의 온도에서 온화한 조건에서 수행되었다. 본 연구에서는 전처리된 바이오매스의 효소당화 조건을 찾아보았다. 볏짚의 경우에 이전의 목질계 바이오매스와 비교하여 당화시간이 다른 것들보다 짧은 것으로 나타났다. SAA(Soaking in Aqueous Ammonia) 전처리 볏짚의 당화는 40~48시간 사이에 종료가 되었고 $50^{\circ}C$에서 높은 글루코스 전환율을 나타냈다. 글루코스 전환율은 효소사용량이 각각 65 FPU/ml과 32 CbU/ml일 때 높았다. 기질 농도가 5%(w/v)일 때 전환율은 72시간 동안 당화 후에 83.8%로 나타났다. SAA 전처리 볏짚의 동시당화발효(SSF; Simultaneous Saccharification and Fermentation) 실험에서는 $40^{\circ}C$에서 높은 에탄올 생산수율을 보였다. 그때의 수율은 48시간에서 33.05%로 나타났다.

감마선 조사 처리에 의한 결명자 줄기의 전처리와 효소가수분해 효과 (Effect of Gamma Ray Irradiation on the Pretreatment and Enzymatic Hydrolysis of Senna tora Stalk)

  • 김조은;공성호;정진태;이옥란;이재원
    • 한국약용작물학회지
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    • 제26권2호
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    • pp.127-133
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    • 2018
  • Background: The demand of recycling renewable agricultural by-products is increasing. Radiation breeding is a method used to improve saccharification efficiency. Thus, we investigated the effect of gamma ray irradiation on the pretreatment and enzymatic hydrolysis of the stalks of Senna tora, an important medicinal plants. Methods and Results: S. tora seeds were irradiated with gamma ray at doses of 100, 200, 300, and 400 Gy. In the pretreated biomass, glucan and lignin content were higher in the M1 ($1^{st}$ generations of irradiation) S. tora stalks than in the M2 ($2^{nd}$ generations of irradiation) stalks, this can be explained by the higher degradation rate in M1. After oxalic acid pretreatment, the concentration of total phenolic compounds (TPCs) in the hydrolysate increased in the gamma ray treated seeds. The highest relative increase rate in crystallinity in the pretreated biomass was observed in M1-400 Gy and M2-100 Gy. The cellulose conversion rate was higher in M1 than in M2, except for 200 Gy. Conclusions: Gamma ray irradiation at an appropriate dose can be used to improve the efficiency of pretreatment and enzymatic hydrolysis, thereby increasing biomass availability.