• 한국가축번식학회지
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• 제21권4호
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• pp.325-333
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• 1997

Recent evidence indicates that growth factors modulate response of mammary epithelial cells to environmental stress. The objective of this study was to examine the cellular and biochemical responses of mammary tissue to environmental stress caused by artificial mastitis. For experimental a, pp.oach, toxins of most mastitis causing organisms(Staph. aureus or Strep. agalactiae) and heat stress(42$^{\circ}C$) were artificially exposed to mammary tissue. Effects of these environmental stresses on cell growth, cell death and heat shock protein synthesis were examined. Lactating mammary tissure were cultured under basal medium(DMEM) su, pp.emented with insulin(10$\mu\textrm{g}$/ml) and aldosterone(1$\mu\textrm{g}$/ml). All treatment groups in heat stress at 42$^{\circ}C$ incubation significantly decreased DNA synthesis rates in comparison with those at 39$^{\circ}C$(P<0.05), however, these decreased DNAa synthesis rates were recovered by addition of adenosine(10$\mu$M) and IGFI(10ng/ml). Similar results were obtained when tissue growth rates were measured by DNA content/tissue. Strep. agalactiae toxin did not significantly decreased DNA content/tissue in comparison with no treatment of bacterial toxin with or without heat stress, however, tended to decrease DNA contents/tissue without heat stress. In the fluorography analysis, heat stress(42$^{\circ}C$ incubation) slightly increased 35S-methoionine labelled 70kd protein synthesis. These results indicate that environmental stress caused by artificial mastitis slightly decreased mammary growth or mammary size, however, these results could be recovered by addition of adenosine and IGF-I.

• Journal of Microbiology and Biotechnology
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• 제33권9호
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• pp.1119-1129
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• 2023

Seeds are colonized by diverse microorganisms that can improve the growth and stress resistance of host plants. Although understanding the mechanisms of plant endophyte-host plant interactions is increasing, much of this knowledge does not come from seed endophytes, particularly under environmental stress that the plant host grows to face, including biotic (e.g., pathogens, herbivores and insects) and abiotic factors (e.g., drought, heavy metals and salt). In this article, we first provided a framework for the assembly and function of seed endophytes and discussed the sources and assembly process of seed endophytes. Following that, we reviewed the impact of environmental factors on the assembly of seed endophytes. Lastly, we explored recent advances in the growth promotion and stress resistance enhancement of plants, functioning by seed endophytes under various biotic and abiotic stressors.

• Journal of Microbiology and Biotechnology
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• 제22권1호
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• pp.118-125
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• 2012

Heat shock proteins (Hsps) play a key role in the cellular defense response to diverse environmental stresses. Here, the role of Hsp genes in the acquisition of thermotolerance in the cyanobacterium Microcystis aeruginosa NIES-298 was investigated. Twelve Hsp-related genes were examined to observe their modulated expression patterns at different temperatures (10, 15, 25, and $35^{\circ}C$) over different exposure periods. HspA and HtpG transcripts showed an up-regulation of expression at low temperatures (10 and $15^{\circ}C$) and high temperature ($35^{\circ}C$), compared with the control ($25^{\circ}C$). To examine their effects upon thermotolerance, we purified recombinant HspA and HtpG proteins. During a thermotolerance study at $54^{\circ}C$, the HspA-transformed bacteria showed increased thermotolerance compared with the control. HtpG also played a role in the defense response to acute heat stress within 30 min. These findings provide a better understanding of cellular protection mechanisms against heat stress in cyanobacteria.

• 한국환경과학회지
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• 제12권6호
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• pp.651-657
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• 2003

In order to examine if arsenic, one of environmental stresses, contributes to hypertension as one of cardiovas cular pathological factors, this study was perfarmed in vivo and in vitro, using intacted or pithed rats and aorta ring preparation, respectively. And also the relationship between expression of heat shock protein (HSP) 90 and vasoactives-induced contractile response was elucidated. To measure blood pressure, the carotid arterial pressure was recorded on physiograph(Grass Co. 79E) connected to strain gauge. On the other hand, contractile response of vascular ring preparation isolated from rat was determined in organ bath and was recorded on physiograph connected to isometric transducer. And HSP was detacted by Western blotting whole cell Iysis. Preganglionic nerve stimulation was increased by 26.0% in arterial pressure of rat treated with arsenic. Vascular contractile response was monitored and HSP were measured by Western blotting of whole Iysis prepared from samples exposed with 0, 0.5, 1, 2 and 4 mM of arsenic for 8 hours. The dose-vascular responses of potassium chloride were augmented by increasing dose of arsenic in the strips exposed to arsenic for 8 hours, and were not augmented for 1, 3, 5 hours. And the response of relaxation of rat aorta induced by histamine was not influenced by arsenic stress. The increase of HSP 90 expression in rat aorta was pronounced at 8 hours after 4 mM of arsenic treatment, but HSP 60 expression was not. Arsenic stress not only increased the expression of HSP 90 in the rat aorta, but also augmented contractions to potassium chloride. These results indicated that arsenic stress was sufficient to induce heat shock protein 90, resulting in increased vascular contractility in rat aorta.

• KSBB Journal
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• 제27권4호
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• pp.268-272
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• 2012

Microbially induced calcite precipitation (MICP) has been explored for protection and consolidation of construction materials such as concrete. In this study, we isolated 54 calcite forming bacteria from concrete pavement and selected 5 isolates which showed high specific urease activity. Also response of the 5 strains against various environmental stresses was examined. BC 4 and BC 5 showed 35% and 26% viability at heat stress ($50^{\circ}C$), respectively. BC 1 and BC 4 maintained 60.4% and 70.4% viability upon osmotic stress (1 M NaCl), respectively. Among the 5 isolates BC 4 had the highest viability upon alkaline stress (pH 10).

• Journal of Plant Biotechnology
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• 제50권
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• pp.232-238
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• 2023

Histone deacetylases (HDACs) play a pivotal role in epigenetic regulation, affecting the structure of chromatin and gene expression across different stages of plant development and in response to environmental stresses. Although the role of HDACs in Arabidopsis and rice has been focused on in extensive research, the role of the HDAC gene family in various medicinal plants remains unclear. In the genome of the balloon flower (Platycodon grandiflorus), we identified 10 putative P. grandiflorus HDAC (PlgHDAC) proteins, which were classified into the three families (RPD3/HDA1, SIR2, and HD2 HDAC families) based on their domain compositions. These HDACs were predicted to be localized in various cellular compartments, indicating that they have diverse functions. In addition, the tissue-specific expression profiles of PlgHDACs differed across different plant tissues, indicating that they are involved in various developmental processes. Furthermore, the expression levels of all PlgHDACs were upregulated in leaves after waterlogging treatment, implying their potential role in coping with waterlogging-induced stress. Overall, our findings provide a comprehensive foundation for further research into the epigenetic regulation of PlgHDACs, and particularly, on their functions in response to environmental stresses such as waterlogging. Understanding the roles of these HDACs in the development and stress responses of balloon flower could have significant implications for improving crop yield and the quality of this important medicinal plant.

• 한국환경복원기술학회지
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• 제19권1호
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• pp.1-11
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• 2016

Currently, urban green space is disappearing due to urbanization, industrialization and various environmental problems including the disruption of the ecology in urban areas. To solve such problems and increase urban green area, roof greening has been suggested as an alternative. Through observing the responses of three plant species (Mukdenia rossii, Dianthus chinensis, and Pachysandra terminalis) plantrd on the soil mixed with Geohumus, this study investigated the effect of Gehumus on soil water content and plant survival. Soil water content of the rooftop soils has been increased when mixed with Geohumus. The responses were proportional to the amount of Geohumus in the mixture. Geohumus exerted a stronger influence on raising soil moisture content for soil A which had lower water-holding capacity. The stress responses of the plants varied in relation to the amount of Geohumus and soil moisture content. The stress response was lowest for Dianthus chinensis and increased in the order of Mukdenia rossii and Pachysandra terminalis. With the highest plant stress, Pachysandra terminalis showed the lowest survival rate among the three species. Without irrigation, the plants survived only for six weeks on green roofs. The survival rate differed depending on the amount of Geohumus mixed. The results of the experiment showed, with some exceptions, that Geohumus helped to improve soil water content, reduce plant stress, and extend plant survival period.

• 한국지반공학회논문집
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• 제35권6호
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• pp.5-15
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• 2019

액상화 평가를 수행할 때 진동전단응력비(CSR)는 일반적으로 지반응답해석 또는 Seed & Idriss의 간편법을 수정한 방법을 통해 산정하고 있다. 본 연구에서는 진동전단응력비 산정방법의 적용성을 분석하기 위하여 1차원 등가선형 지반응답해석을 수행한 후 미연방도로국(FHWA), 일본도로협회(JRA), 국내설계기준(KDS) 등에서 제안한 방법을 적용하여 진동전단응력비를 산정하였다. 연구결과, 국내설계기준(KDS)으로 산정한 진동전단응력비가 해석 결과와 가장 큰 오차를 나타내었다. 그 이유는 국내설계기준의 경우 깊이에 따른 응력감소계수를 최대진동전단응력의 비가 아닌 최대지반가속도의 비로 정의하는 오류가 있기 때문이다.

• Computers and Concrete
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• 제1권1호
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• pp.77-98
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• 2004

This paper presents a numerical model for simulating the nonlinear response of reinforced concrete (RC) shear walls subject to cyclic loadings. The material behavior of cracked concrete is described by an orthotropic constitutive relation with tension-stiffening and compression softening effects defining equivalent uniaxial stress-strain relation in the axes of orthotropy. Especially in making analytical predictions for inelastic behaviors of RC walls under reversed cyclic loading, some influencing factors inducing the material nonlinearities have been considered. A simple hysteretic stress-strain relation of concrete, which crosses the tension-compression region, is defined. Modification of the hysteretic stress-strain relation of steel is also introduced to reflect a pinching effect depending on the shear span ratio and to represent an average stress distribution in a cracked RC element, respectively. To assess the applicability of the constitutive model for RC element, analytical results are compared with idealized shear panel and shear wall test results under monotonic and cyclic shear loadings.

• Nuclear Engineering and Technology
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• 제40권3호
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• pp.239-250
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• 2008

If there is a water flow with a range of temperature inside a pipe, the wanner water tends to float on top of the cooler water because it is lighter, resulting in the upper portion of the pipe being hotter than the lower portion. Under these conditions, such thermal stratification can play an important role in the aging of nuclear power plant piping because of the stress caused by the temperature difference and the cyclic temperature changes. This stress can limit the lifetime of the piping, even leading to penetrating cracks. Investigated in this study is the effect of thermal stratification on the structural integrity of the pressurizer surge line, which is reported to be one of the pipes most severely affected. Finite element models of the surge line are developed using several element types available in a general purpose structural analysis program and stress analyses are performed to determine the response characteristics for the various types of top-to-bottom temperature differentials due to thermal stratification. Fatigue analyses are also performed and an allowable environmental correction factor is suggested.