• Journal of Environmental Science International
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• v.7 no.5
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• pp.599-605
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• 1998

The purpose of this work was to examine whether X-Y chromosome dissociation in the primary spermatocytes of mice could be used as an in vivo short-term assaying system that detect environmental mutagens. Four alkylating agents(EMS, MMS, MMC and MNNG) which were known as strong mutagens were administered to BALB/c male mice 3-4 months old. In the control group, the mean frequencies of previously dissociated X and Y chromosomes and autosomes were 7.17% and 2.12%, respectively. Compared to the control group, mutagen-treated groups have no significant differences in dissociation rate of autosomes, while these poops were about 1.2-2.5 times higher in the frequencies of X-Y dissociation. Generally, X-Y dissociation frequency increased consistently with the concentration of mutagens whereas the tendency of autosome dissociation frequency was variable among several mutagens. These results suggest that X-Y dissociation in the primary spermatocytes of mice is applicable as an vivo short-term assaying system for environmental mutagens. There were significantly distinct increase in dissociation of X-Y chromosome in both the hybrid and parents but the X-Y previous dissociation of hybrid appeared higher frequency than BALB /c and wild mice. These results indicate that the factor related to binding X-Y chromosome is specific to strains.

• Toxicological Research
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• v.11 no.1
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• pp.51-55
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• 1995

The present experiment was carried out to investigate whether X and Y chromosome dissociation in the primary spermatocytes of mice can be used as an in vivo assaying system that detect environmental mutagens. For this purpose, alkylating agents (EMS, MMS and MMC), which are strong mutagens, were administered to ICR male mice 12-15 weeks old. The mean frequencies of previously dissociated X-Y chromosomes and autosomes of the control group were 7.34-7.45% and 0.92-1.04%, respectively. The frequencies of X-Y dissociation in the mutagen-treated groups with 10.0 mM EMS and 5.0 mM MMS were about 3.3-4.6 times higher than that in the control group, but there were no significant differences in dissociation of autosomes in both the control and the mutagen-treated groups. These results suggest that X-Y dissociation in the primary spermatocytes of mice can be used as an in vivo short-term assaying system for environmental mutagens.

• Archives of Pharmacal Research
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• v.11 no.1
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• pp.1-6
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• 1988

The use of blue cotton for detecting polycyclic aromatic mutagens in environmental samples (foods, human excretions, river water, etc) is reviewed. Since the invention of blue cotton has its origin in studies of mutagen modulators, these studies are also briefly reviewed.

• Toxicological Research
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• v.16 no.1
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• pp.53-57
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• 2000

A mutagenicity testing system using a specific locus mutation of Bombyx mori was introduced from National Institute of Genetics in Japan. In the system, mutagenicity could be detected by the egg color manifested by the pe and/or re genes, which is a kind of recessive visible mutation of the insect. The efficiency to detect mutagenicity of the system was examined and improved. To promote the sensitivity of the system to mutagens, eight varieties of Bombyx mori were tested for their sensitivity to two mutagens, EMS and MMC. Two varieties of the silkworm, N12 and C5, were finally selected as the most sensitive ones. The most sensitive development stage of the silkworm to mutagens was mid and late pupal stages for female and male, respectively. The system will be applied to test unknown mutagens after some more detail examination about its sensitivity.

• Proceedings of the Korea Society of Environmental Toocicology Conference
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• 2003.10a
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• pp.175-175
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• 2003

Phthalocyanine, a water soluble porphyrin derivative and dye, is known to inhibit the mutagenic and carcinogenic actions of compounds having polycyclic structures, e.g. heterocyclic amines. There is evidence that this adsorbent effect shows by a complex formation between the porphyrin-like structure of phthalocyanine and the planar molecular surfaces of theses compounds. That phthalocyanine can form an insoluble material when mixed with chitosan, a polyglucosamine, and that the solid chitosan-phthalocyanine, named Eco-Blue, thus prepared can efficiently adsorb polycyclic mutagenic compounds. The adsorption was experimented by UV/VIS spectrometry. The adsorbent effects of mutagens and carcinogens was identified by Gas chromatography (GC) and Ames Test. The adsorbed polycyclic mutagens were elutable with buffer, but only to small extents. Chitosan-phthalocyanine may be expected to be useful as an adsorbent against polycyclic mutagens and carcinogens.

• Journal of the Korean Society of Food Science and Nutrition
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• v.28 no.3
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• pp.599-606
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• 1999

Water extracts of persimmon leaf tea(PLTE), green tea(GTE) and oolong tea(OTE), at the con centration used for human consumption, were examined for inhibitory effects on the mutagenicity of major classes of dietary and environmental mutagens including indirect acting mutagens, B[ ]P (benzo[ ]pyrene), IQ(2 amino 3 methylimidazo[4,5 f]quinoline), 2 AA(2 aminoanthracene) in the presence of S9 mix and direct acting mutagen, 4 NQO(4 nitroquinoline 1 oxide) without S9 mix, using the modified Ames Salmonella/microsome assay. PLTE, GTE and OTE showed very potent and concentration dependent antimutagenic effects against indirect acting mutagens B[ ]P and IQ. At the maximum concentration(16,200 g/plate) of each tea extract, number of colonies decreased in a dose dependent manner up to 82~100%. Similar inhibition of PLTE, GTE and OTE were seen at higher concentration in the mutagenicity of the 2 AA following an initial increase in the activity at lower concentration. However, the mutagenicity of the direct acting mutagen 4 NQO were not suppressed at lower concentration of the three tea extracts, and higher concentration of the tea extracts enhanced mutagenic activity of the mutagen. There were no differences in the mode of antimutagenesis between PLTE, GTE, and OTE, in both Salmonella typhimurium TA98 and TA100 strains against the same mutagen. In conclusion, the water extracts of persimmon leaf tea, green tea and oolong tea possess marked antimutagenic potential against a variety of important dietary and environmental indirect acting mutagens, but the activity was not observed against the direct acting mutagens. These results suggest that the mode of inhibitory action may not have resulted from direct interaction between tea extracts and the mutagens, but rather from indirect metabolic inactivation of mutagens by tea extracts.

• Environmental Analysis Health and Toxicology
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• v.7 no.3_4
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• pp.69-79
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• 1992

Recently, concerns of water pollution and health risks caused by synthetic detergents have emerged, as the use of various detergents has increased It has been suggested that some surfactants are cocarcinogens. The surfactants tested were linear alkylbenzene sulfonate, sodium lauryl sulfate, polyoxyethylene sorbitan monooleat (tween 80), and the mutagens were 1-nitropyrene, N -methyl- N'-nitro-N -nitrosoguanidine, benzo (a) pyrene, and aflatoxin B$_1$. This study was undertaken to investigate the effects of surfactants on the activity of mytagens using the Ames mutagenic assay with Salmonella typhimurium TA98, TA100. The results were summarized as follows: 1. The surfactants have no mutagenic activity of themselves. 2. Higher doses of surfactants than 100 $\mu\textrm{g}$/plate reduced the number of revertants. It is assumed that the reduction would inhibited cell growth. 3. When the comutagenic ratio is defined as the ratio between mutagenic activity itself and the activity with mutagen and surfactant (drinking water quality standard), LAS showed the comutagenic ratio 0.86-1.17 and SLS 0.74-1.10 as well. According to the comparisons, it could not be recognised for the comutagenicity of drinking water quality standard of surfactant. 4. As increasing the amount of mutagens, the designated amount of surfactant did not affected the mutagen's activity statistically. From the above result, syunthetic surfactants do not present mutagenicity and comutagenicity in the microbial assay.

• Environmental Mutagens and Carcinogens
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• v.24 no.1
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• pp.10-14
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• 2004

• Journal of Forest and Environmental Science
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• v.25 no.3
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• pp.147-155
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• 2009

Higher plants provide valuable genetic assay systems for screening and monitoring environmental pollutants. They are now recognized as excellent indicators of mutagenic effects of environmental chemicals and are applicable for the detection of environmental mutagens both indoor and outdoor. 2,4-dichlorophenoxyacetic acid (2,4-D) is a herbicide commonly used in agriculture. The residues of 2,4-D are present in air, water, soil and edible plants. It constitutes a real hazard to the public health because it's wide spread use in agriculture. Genotoxic effects of 2,4-D on plant cells and potential of higher plants as a biomonitoring system for detecting chemical mutagens are evaluated. It is recommended that higher plant systems have been accepted by regulatory authorities as an alternative biomonitoring system for the detection of possible genetic damage resulting from pollution and the use of environmental chemicals.

• Environmental Mutagens and Carcinogens
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• v.22 no.1
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• pp.1-11
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• 2002