• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2003.06a
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• pp.82-89
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• 2003

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2002.10a
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• pp.66-66
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• 2002

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2002.10a
• /
• pp.62-62
• /
• 2002

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2002.10a
• /
• pp.97-97
• /
• 2002

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2005.06a
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• pp.336-336
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• 2005

• Proceedings of the Korean Society for Applied Microbiology Conference
• /
• 2005.06a
• /
• pp.340-340
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• 2005

• Proceedings of the Korea Society of Environmental Biology Conference
• /
• 1997.12a
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• pp.6-6
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• 1997

• Journal of Microbiology and Biotechnology
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• v.16 no.3
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• pp.386-390
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• 2006

A green fluorescent protein-based Pseudomonas putida reporter was successfully constructed and shown to be capable of detecting oxidative stress. In this whole-cell reporter, the promoter of the paraquat-inducible ferredoxin-$NADP^+$ reductase (fpr) was fused to a promoterless gfp gene on a broad-host-range promoter probe vector. Pseudomonas putida KT2440 harboring this reporter plasmid exhibited an increased level of gfp expression in the presence of redox-cycling agents (paraquat and menadione), hydrogen peroxide, and potential environmental pollutant chemicals such as toluene, paint thinner, gasoline, and diesel. Induction of fpr in the presence of these chemicals was confirmed using Northern blot analysis.

• Journal of Microbiology and Biotechnology
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• v.22 no.8
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• pp.1113-1117
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• 2012

Shigella sonnei is a causal agent of fever, nausea, stomach cramps, vomiting, and diarrheal disease. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection of S. sonnei using a primer pair based on the methylase gene for the amplification of a 325 bp DNA fragment. The qPCR primer set for the accurate diagnosis of Shigella sonnei was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.

• Journal of Korean Society on Water Environment
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• v.25 no.1
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• pp.136-141
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• 2009

Inactivation rates between enterococci and total coliforms were compared in order to find the suitability of enterococci as an indicator microorganism under various experiment conditions - freshwater and/or seawater, indoor and/or outdoor conditions. In case of indoor laboratory experiments, inactivation rates of enterococci ($k_D$: 0.050~0.082) were faster than those of total coliforms ($k_D$: 0.034~0.045) in freshwater matrix. In seawater matrix, however, survival rate of enterococci was longer than that of total coliforms at two out of three experiments in indoor condition. When incubated in outdoor conditions, enterococci were inactivated significantly more rapidly than total coliforms both in freshwater and seawater matrices. With these results, enterococci appear to be less suitable than total coliforms in terms of inactivation rates.