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http://dx.doi.org/10.4014/jmb.1111.11006

A Novel Marker for the Species-Specific Detection and Quantitation of Shigella sonnei by Targeting a Methylase Gene  

Cho, Min Seok (National Academy of Agricultural Science, Rural Development Administration)
Ahn, Tae-Young (Department of Microbiology, Dankook University)
Joh, Kiseong (Department of Bioscience and Biotechnology, Hankuk University of Foreign Studies)
Kwon, Oh-Sang (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research Environmental Research Complex)
Jheong, Won-Hwa (Water Supply and Sewerage Research Division, Environmental Infrastructure Research Department, National Institute of Environmental Research Environmental Research Complex)
Park, Dong Suk (National Academy of Agricultural Science, Rural Development Administration)
Publication Information
Journal of Microbiology and Biotechnology / v.22, no.8, 2012 , pp. 1113-1117 More about this Journal
Abstract
Shigella sonnei is a causal agent of fever, nausea, stomach cramps, vomiting, and diarrheal disease. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the specific detection of S. sonnei using a primer pair based on the methylase gene for the amplification of a 325 bp DNA fragment. The qPCR primer set for the accurate diagnosis of Shigella sonnei was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.
Keywords
Detection; diagnosis; quantitation; methylase; Shigella sonnei;
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