• 제목/요약/키워드: environmental microbiology

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Two New Species in the Family Cunninghamellaceae from China

  • Zhao, Heng;Zhu, Jing;Zong, Tong-Kai;Liu, Xiao-Ling;Ren, Li-Ying;Lin, Qing;Qiao, Min;Nie, Yong;Zhang, Zhi-Dong;Liu, Xiao-Yong
    • Mycobiology
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    • 제49권2호
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    • pp.142-150
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    • 2021
  • The species within the family Cunninghamellaceae are widely distributed and produce important metabolites. Morphological studies along with a molecular phylogeny based on the internal transcribed spacer (ITS) and large subunit (LSU) of ribosomal DNA revealed two new species in this family from soils in China, that is, Absidia ovalispora sp. nov. and Cunninghamella globospora sp. nov. The former is phylogenetically closely related to Absidia koreana, but morphologically differs in sporangiospores, sporangia, sporangiophores, columellae, collars, and rhizoids. The latter is phylogenetically closely related to Cunninghamella intermedia, but morphologically differs in sporangiola and colonies. They were described and illustrated.

습지에서 분리한 질소고정 세균인 Mycobacterium hominis sp. AKC-10의 특성 (Characterization of a Nitrogen Fixing Bacteria Mycobacterium hominis sp. AKC-10 Isolated from the Wetland)

  • 홍선화;신기철;이은영
    • 한국미생물·생명공학회지
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    • 제38권3호
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    • pp.302-307
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    • 2010
  • Nitrogen is an element need to grow plants growth. Plants take up nitrogen in the form of nitrate or ammonium. Most of plants absorb nitrogen source as fertilizers. But from 50 to 70% of fertilizers applied were washed away. This study was conducted to isolate free-living nitrogen fixing bacteria from reed and to examine its beneficial traits for developing sustainable biofertilizers. Enriched consortium obtained from a reed in Ansan was developed for the fixing of nitrogen. Nitrogen fixing bacteria isolated from an enriched culture in Congo Red Medium was analyzed by 16s rDNA sequencing. AKC-10 was isolated and shown to have excellent nitrogen fixing ability. The optimum conditions of nitrogen fixing ability were $25^{\circ}C$ ($237.50{\pm}39.65\;nmole{\cdot}mg-protein^{-1}{\cdot}h^{-1}$ and pH 7 ($168.335{\pm}12.84$ nmole/hr mg-protein). It was identified as Microbacterium hominis [(AKC-10 (similarity : 99%)]. This strain was had to IAA (indole-3-acetic acid) productivity and ACC(1-aminocyclopropane-1-carboxylic acid) deaminase activity. Therefore, Microbacterium hominis AKC-10 stimulated plant development in the soil, enhancing the efficiency of remediation.

Additions to the Knowledge of the Fungal Order Eurotiales in Korea: Eight Undescribed Species

  • Thuong T. T. Nguyen;Ki Hyun Kang;Dong Hee Kim;Su Jin Kim;Hye Yeon Mun;Wonsu Cheon;Hyang Burm Lee
    • Mycobiology
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    • 제51권6호
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    • pp.417-435
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    • 2023
  • Eurotiales is a relatively large order of Ascomycetes, well-known for their ability to produce secondary metabolites with potential beneficial applications. To understand their diversity and distribution, different environmental sources including soil, freshwater, insect, and indoor air were investigated. Eight strains of Eurotiales were isolated and identified based on their morphological characters and a multi-gene phylogenetic analysis of the ITS, BenA, CaM, and RPB2 regions. We identified eight taxa that were previously not reported from Korea: Aspergillus baeticus, A. griseoaurantiacus, A. spinulosporus, Penicillium anthracinoglaciei, P. labradorum, P. nalgiovense, Talaromyces atroroseus, and T. georgiensis. Detailed descriptions, illustrations, and phylogenetic tree for the eight new records species are presented, and information regarding the records is also discussed.

Isolation and Characterization of a Novel Polysaccharide Producing Bacillus polymyxa A49 KCTC 4648P

  • Ahn, Sung-Gu;Suh, Hyun-Hyo;Lee, Chang-Ho;Moon, Seong-Hoon;Kim, Hee-Sik;Ahn, Keug-Hyun;Kwon, Gi-Seok;Oh, Hee-Mock;Yoon, Byung-Dae
    • Journal of Microbiology and Biotechnology
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    • 제8권2호
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    • pp.171-177
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    • 1998
  • The strain A49, which produces a new type of extracellular polysaccharide was isolated from soil samples. From morphological, physiological and biochemical tests, the strain A49 was identified as a Bacillus polymyxa and named Bacillus polymyxa A49. Bacillus polymyxa A49 was found to produce a highly viscous extracellular polysaccharide when grown aerobically in a medium containing glucose as the sole source of carbon. The polysaccharide (A49 POL) showed a homogeneous pattern on gel permeation chromatography (GPC) and its molecular weight was estimated to be about 1.6 mega dalton (mDa). The FT-IR spectrum of A49-POL revealed typical characteristics of polysaccharides. As a result of investigations with HPLC and carbozole assay, A49-POL was found to consist of L-fucose, D-galactose, D-glucose, D-mannose, and D-glucuronic acid, with the molar ratio of these sugars being approximately 1:2:7:50:12. Rheological analysis of A49 POL revealed that it is pseudoplastic and has a higher apparent viscosity at dilute concentrations than does xanthan gum. The consistancy factor of A49 POL was found to be higher, and the flow index of A49 POL lower, than xanthan gum. Its apparent viscosity was comparatively unstable at various temperatures. the A49 POL showed the highest apparent viscosity at pH 3. When salts were added to A49 POL solution, the solution was compatible with up to 10% KCl, 35% NaCl, 55% $CaCl_2$, 55% $MgCl_2$, 55% $K_2HPO_4$, and 110% $Ca({NO_3})_2$, respectively.

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Analysis of Learning Process and Achievement for Environmental Microbiology Taught in English as a Basis of Environmental Science and Technology

  • Koh, Sung-Cheol;Ekpeghere, Kalu I.
    • 공학교육연구
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    • 제13권2호
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    • pp.16-21
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    • 2010
  • The objective of this study was to evaluate Environmental microbiology (EM) as a basic course for the environmental engineering program of Korea Maritime University which has been accredited by Accreditation Board for Engineering Education of Korea (ABEEK). Evaluation result of the lecture objectives was more than the average (3.5/5.0). The overall score for learning achievement assessment was more than average level of learning achievement (3.4/5.0). Interestingly, engineering design implementation regarding a specific topic as an extensive learning process has proven to be more effective (3.5/5.0). Some of effective class improvement strategies suggested were to encourage students to participate in the class with self-motivation and accountability: preview and review of the lecture, team presentation in English-speaking settings, and submitting project report written in English. In the future class of EM, test and evaluation of application capability of EM and environmental biotechnology (EB) theories to engineering problem solving should be emphasized.

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Endomicrobial Community Profiles of Two Different Mealybugs: Paracoccus marginatus and Ferrisia virgata

  • Jose, Polpass Arul;Krishnamoorthy, Ramasamy;Gandhi, Pandiyan Indira;Senthilkumar, Murugaiyan;Janahiraman, Veeranan;Kumutha, Karunandham;Choudhury, Aritra Roy;Samaddar, Sandipan;Anandham, Rangasamy;Sa, Tongmin
    • Journal of Microbiology and Biotechnology
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    • 제30권7호
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    • pp.1013-1017
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    • 2020
  • Mealybugs (Hemiptera: Coccomorpha: Pseudococcidae) harbor diverse microbial symbionts that play essential roles in host physiology, ecology, and evolution. In this study we aimed to reveal microbial communities associated with two different mealybugs, papaya mealybug (Paracoccus marginatus) and two-tailed mealybug (Ferrisia virgata) collected from the same host plant. Comparative analysis of microbial communities associated with these mealybugs revealed differences that appear to stem from phylogenetic associations and different nutritional requirements. This first report on both bacterial and fungal communities associated with these mealybugs provides a preliminary insight on factors affecting the endomicrobial communities.