• Title/Summary/Keyword: environmental DNA

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POLYCHLORINATED BIPHENYLS INDUCE ARYL HYDROCARBON RECEPTOR-INDEPENDENT APOPTOSIS OF MOUSE SPLEEN CELL

  • Jeon, Young-Jin;Youk, Eun-Soo;Lee, Sang-Han;Jaehong Suh;Na, Yong-Joo;Kim, Hwan-Mook
    • Proceedings of the Korean Society of Toxicology Conference
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    • 2002.05a
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    • pp.79-79
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    • 2002
  • Polychlorinated biphenyls (PCBs) are ubiquitous environmental contaminants, and many of their toxic effects, including their immunotoxicities, are mediated by the activation of aryl hydrocarbon receptor (AhR). We previously reported that Aroclor 1254, one of the most widely used PCB mixtures, increased DNA fragmentation in mouse spleen cells, suggesting that apoptosis was correlated with the immunotoxicity of PCB (Yoo et al., 1997).(omitted)

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분자생물학적 기법을 사용한 질산화유도 반응기내 군집동태변화

  • Jo, Sun-Ja;Jeong, Yong-Ju;Kim, Jeong-Cheol;Lee, Sang-Jun
    • Proceedings of the Korean Environmental Sciences Society Conference
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    • 2005.05a
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    • pp.247-248
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    • 2005
  • 본 연구는 서로 다른 조건에서 질산화를 유도한 반응기의 군집동태를 살피기 위해 RAPD, ARDRA, DGGE와 같은 기법들을 적용시켜 반응기 초기의 슬러지 구성 군집의 상태 와 질산화 유도후의 군집 변화를 살펴보고자 하였다. 결과적으로 1.5 kb정도의 165 rDNA를 이용한 RAPD와 ARDRA에서는 RAPD에 의한 군집 Patterns변화가 훨씬 다양했으며, 250 bP정도의 PCR산물로 분리를 시도한 DGGE에서도 비교적 예상했던 바와 같이 군집이 단순화되는 양상을 볼 수 있었다.

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Bacterial Community Analysis during Composting of Garbage using Denaturing Gradient Gel Electro-phoresis (Denaturing Gradient Gel Electrophoresis를 이용한 음식물 쓰레기 퇴비화 세균 군집 분석)

  • Ryu Hee Wook;Cho Kyung-Suk
    • Microbiology and Biotechnology Letters
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    • v.33 no.3
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    • pp.226-230
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    • 2005
  • The microbial community during composting of gargage was analyzed using 16S rDNA PCR - DGCE (denaturing gradient gel electrophoresis). Pseudomonas spp. was found throughout the process, and thermophilic Bacillus spp. was dominated at the thermophilic stage. Six thermophilic bacteria were isolated and identified as B. caldoxylolyticus, B. thermoalkalophilus, and B. thermodenitrificans.