Browse > Article

Bacterial Community Analysis during Composting of Garbage using Denaturing Gradient Gel Electro-phoresis  

Ryu Hee Wook (Department of Chemical and Environmental Engineering, Soongsil University)
Cho Kyung-Suk (Department of Environmental Science and Engineering, Ewha Womans University)
Publication Information
Microbiology and Biotechnology Letters / v.33, no.3, 2005 , pp. 226-230 More about this Journal
Abstract
The microbial community during composting of gargage was analyzed using 16S rDNA PCR - DGCE (denaturing gradient gel electrophoresis). Pseudomonas spp. was found throughout the process, and thermophilic Bacillus spp. was dominated at the thermophilic stage. Six thermophilic bacteria were isolated and identified as B. caldoxylolyticus, B. thermoalkalophilus, and B. thermodenitrificans.
Keywords
Composting; bacterial community; denaturing gradient gel electrophoresis; garbage;
Citations & Related Records

Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Dees, P. M. and W. C. Ghiorse. 2001. Microbial diversity in hot synthetic compost as revealed by PCR-amplified rRNA sequences from cultivated isolates and extracted DNA. FEMS Microbiol. Ecol. 35: 207-216   DOI   ScienceOn
2 Eilers, H., J. Pernthaler, F. O. Glockner, and R. Amann. 2000. Culturability and in situ abundance of pelagic bacteria from the North Sea. Appl. Environ. Microbiol. 66: 3044-3051   DOI   ScienceOn
3 James, S. A, J. Cal, I. N. Roberts, and M. D. Collins. 1997. A phylogenetic analysis of the genus Saccharomyces based on 18S rRNA gene sequences: description of Saccharomyces kunashirensis sp. nov. and Saccharomyces martiniae sp. nov. Int. J. Syst. Bacteriol. 47: 453-460   DOI   PUBMED   ScienceOn
4 Klamer, M. and E. Baath. 1998. Microbial community dynamics during composting of straw material studied using phospholipid fatty acid analysis. FEMS Microbial. Ecol. 27: 9-20   DOI   ScienceOn
5 Nakasaki, K., H. Yaguchi, Y. Sasaki, and H. Kubata. 1985. Effects of C/N ratio on thermophilic composting of garbage. J. Ferment. Bioeng. 63: 43-45
6 Bowman, J.P., S.A. McCammon, M.V. Brown, D.S. Nichols, and T.A. McMeekin. 1997. Diversity and association of psychrophilic bacteria in antarctic sea ice. Appl. Environ. Micorbiol. 63: 3068-3078
7 Ishii, K., M. Fukui, and S. Takii. 2000. Microbial succession during a composting process as evaluated by denaturing gradient gel electrophoresis analysis. J. Appl. Microbiol. 89: 768-777   DOI   ScienceOn
8 Sakala, R. M., Y. Kato, H. Hayashidani, M. Murakami, C. Kaneuchi, and M. Ogawa. 2002. Lactobacillus fuchuensis sp. nov., isolated from vacuum-packaged refrigerated beef. Int. J. Syst. Evol. Microbiol. 52(Pt 4): 1151-1154   DOI   ScienceOn
9 Franke-Whittle, I. H., S.H . Klammer, and H. Insam. 2005. Design and application of an oligonucleotide microarray for the investigation of compost microbial communities. J. Microbiol. Methods 62: 37-56   DOI   ScienceOn
10 Blanc, M., L. Marilley, T. Bela, and M. Aragno. 1999. Thermophilic bacterial communities in hot composts as revealed by most probable number counts and molecular (16S rDNA) methods. FEMS Microbiol. Ecol. 28: 141-149   DOI   ScienceOn
11 Bae, Y. J., H. Kaneko, and F. Fujita, 1993. Profile of microbial numbers and growth activity in composting process. J. KOWREC 1: 59-68
12 Loeffler, J., H. Hebart, S. Magga, D. Schmidt, L. Klingspor, J. Tollemar, U. Schumacher, and H. Einsele. 2000. Identification of rare Candida species and other yeasts by polymerase chain reaction and slot blot hybridization. Diagn. Microbiol. Infect. Dis. 38: 207-212   DOI   ScienceOn
13 Nakasaki, K., M. Sasaki, M. Shoda, and H. Kubota. 1985. Changes in microbial numbers during thermophilic composting of sewage sludge with reference to C02 evolution rate. Appl. Environ. Microbiol. 53: 1118-1124
14 Nakasaki, K., Y. Hakano, T. Akiyama, M. Shoda, and H. Kubota. 1987. Oxygen diffusion and microbial activity in the composting of dehydrated sewage sludge cakes. J. Ferment. Bioeng. 65: 43-48
15 Wiedmann, M., D. Weilmeier, S.S. Dineen, R. Ralyea, and K. J. Boor. 2000. Molecular and phenotypic characterization of Pseudomonas spp. isolated from milk. Appl. Environ. Microbiol. 66: 2085-2095   DOI   ScienceOn
16 Juteau, P., R. Larocque, D. Rho, and A. LeDuy. 1999. Analysis of the relative abundance of different types of bacteria capable of toluene degradation in a compost biofilter. Appl. Microbiol. Biotechnol. 52: 863-868   DOI   ScienceOn
17 Kowalchuk, G. A., Z. S. Naoumenko, P. J. L. Derikx, A. Felske, J. R. Stephen, and I. A. Arkhipchenko. 1999. Molecular analysis of ammonia-oxidizing bacteria of the Lsubdivision of the class Proteobacteria in compost and composted materials. Appl. Environ. Microbiol. 65: 396-403   PUBMED
18 Tang, J. C., T. Kanamori, Y. Inoue, T. Yasuta, S. Yoshida, A. Katayama. 2004. Changes in the microbial community structure during thermophilic composting of manure as detected by the quinone profile method. Proc. Biochem. 39: 1999-2006   DOI   ScienceOn
19 Pedro, M.S., S. Haruta, M. Hazaka, R. Shimada, C. Yoshida, K. Hiura, M. Ishii, and Y. Igarashi, Y. 2001. Denaturing gradient gel electrophoresis analyses of microbial community from field-scale composter. J. Biosci. Bioeng. 91: 159-165   DOI   PUBMED
20 Stougaard, P., F. Jorgensen, M. G. Johnsen, and O. C. Hansen. 2002. Microbial diversity in ikaite tufa columns; an alknae, cold ecological niche in Greenland. Environ. Microbiol. 4: 487-493   DOI   ScienceOn
21 Finstein, M. S. and M. L. Morris. 1975. Microbiology of municipal solid waste composting. Adv. Appl. Microbial. 19: 113-151   DOI   PUBMED
22 Coates, J. D., K. A. Cole, R. Chakraborty, S. M. O'Connor, and L. A. Achenbach. 2002. Diversity and ubiquity of bacteria capable of utilizing humic substances as electron donors for anaerobic respiration, Appl. Environ. Microbiol. 68: 2445-2452   DOI   ScienceOn
23 Herrmann R. F. and J. F. Shann. 1997. Microbial community changes during the composting of municipal solid waste. Microb. Ecol. 33: 78-85   DOI   ScienceOn
24 An, Y. J., Y. H. Joo, I. Y. Hong, H. W. Ryu, and K. S. Cho. 2004. Microbial characterization of toluene-degrading denitrifying consortia obtained from terrestrial and marine ecosystems. Appl. Microbiol. Biotechnol. 65: 611-619   PUBMED
25 Guidi, G, A. Pera, M. Giovannetti, G. Poggio, and M. Bertoldi. 1988. Variations of soil structure and microbial population in a compost amended soil. Plant Soil 106: 113-119   DOI
26 Nakasaki, K., M. Shoda, and H. Kubota. 1986. Effect of bulking agent on the reaction rate of thermophilic sewage sludge composting. J. Ferment. Bioeng. 64: 539-544
27 Gajdos, R. 1992. The use of organic waste materials as organic fertilizers recycling of plant nutrients. Acta Hortic. 302: 325-331
28 Schloss, P. D., A. G. Hay, D.B. Wilson, and L. P. Walker. 2003. Tracking temporal changes of bacterial community fingerprints during the initial stages of composting. FEMS Microbiol. Ecol. 46: 1-9   DOI   ScienceOn