• Title/Summary/Keyword: entrapment

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Improvement of $\gamma-Aminobutyric$ Acid (GABA) Production Using Cell Entrapment of Lactobacillus brevis GABA 057

  • Choi Soo-Im;Lee Jae-Won;Park Sang-Min;Lee Moo-Young;Ji Geun-Eog;Park Myeong-Soo;Heo Tae-Ryeon
    • Journal of Microbiology and Biotechnology
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    • v.16 no.4
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    • pp.562-568
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    • 2006
  • GABA $(\gamma-aminobutyric\;acid)$ is the principal inhibitory neurotransmitter in the brain. For the efficient production of GAB A, a semi continuous cell entrapment system using Lactobacillus brevis GABA 057 was optimized, including the substrate concentration, bead-stabilizing additives, and reaction conditions. The converted monosodium glutamate (MSG), which was added as a substrate for glutamic acid decarboxylase (GAD), increased from 2% (w/v) to 12% (w/v). The addition of isomaltooligosaccharide to the alginate beads also increased the stability of the entrapped L. brevis and GABA productivity. Consequently, when optimal conditions were applied, up to 223 mM GABA could be produced from 534 mM MSG after 48 h of reaction by using alginate-beadencapsulated L. brevis GABA 057.

Development of the Selection Technique of Entrapment Materials for the Viability Improvement of Entrapped Bifidobacteria (포집된 Bifidobacteria의 생존력 증대를 위한 세포포집재료의 선별기술 개발)

  • 이기용;우창재;배기성;허태련
    • KSBB Journal
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    • v.15 no.1
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    • pp.1-8
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    • 2000
  • The diffusion effect of simulated gastric juices into the various alginate vessel containing each biopolymer such as 0.3% soluble starch, whey, corn starch, agar, locust bean gum, guar gum, gum arabic, pectin, gelatin and 0.15% xanthan gum was tested by measuring the change of pH in the vessel. The degree of viability of bifidobacteria entrapped in each bead containing biopolymers was corresponded with the degree of diffusion inhibition of hydrogen into the each vessel. Therefore, The determination of diffusion inhibition of simulated gastric juices into the various vessel by measuring the change of pH in the vessel may be effectively used as the simple method to select the optimal entrapment lattice for the improvement of bifidobacteria viability. Bifidobacteria entrapped in alginate bead containing 0.15% xanthan gum whose lattice showed the lowest hydrogen diffusion were more significantly tolerant against bile salts and hydrogen peroxide than untrapped bifidobacteria. It was also observed that the viability of bifidobacteria entrapped in bead was nto nearly changed in milk adjusted pH 4.5 with organic adids at $4^{\circ}C$ for 10 days. Therefore, use of alginate containing 0.15% xanthan gum as a cell matrix for entrapping bifidobacteria was expected to improve the viability of bididobacteria in fermented milk products and develop the high value-added products.

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Pudendal nerve entrapment syndrome caused by ganglion cysts along the pudendal nerve

  • Kim, Young Je;Kim, Du Hwan
    • Journal of Yeungnam Medical Science
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    • v.38 no.2
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    • pp.148-151
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    • 2021
  • Pudendal nerve entrapment (PNE) syndrome refers to the condition in which the pudendal nerve is entrapped or compressed. Reported cases of PNE associated with ganglion cysts are rare. Deep gluteal syndrome (DGS) is defined as compression of the sciatic or pudendal nerve due to a non-discogenic pelvic lesion. We report a case of PNE caused by compression from ganglion cysts and treated with steroid injection; we discuss this case in the context of DGS. A 77-year-old woman presented with a 3-month history of tingling and burning sensations in the left buttock and perineal area. Ultrasonography showed ganglion cystic lesions at the subgluteal space. Magnetic resonance imaging revealed cystic lesions along the pudendal nerve from below the piriformis to the Alcock's canal and a full-thickness tear of the proximal hamstring tendon. Aspiration of the cysts did not yield any material. We then injected steroid into the cysts, which resolved her symptoms. Steroid injection into a ganglion cyst should be considered as a treatment option for PNE caused by ganglion cysts.

Performing Ultrasound-Guided Pharmacopuncture and Acupotomy for Nerve Entrapment in the Upper Extremity: A Guide for Teaching Procedural Skills

  • Taeseong Jeong;Eunbyul Cho;Sungha Kim;Seunghyun Oh;Suhak Kim;Jeongsu Park;Sungchul Kim
    • Journal of Acupuncture Research
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    • v.41 no.2
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    • pp.135-141
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    • 2024
  • The use of ultrasound (US)-guided interventions has rapidly increased in Korean medicine (KM) to ensure the safety and accuracy of invasive procedures, such as pharmacopuncture and acupotomy. Although hands-on training is important for the acquisition of skills, it requires considerable time and cost. A detailed guide on the procedure and treatment regions is needed to ensure hygiene and safety during US-guided procedures in KM practice. In this study, we present the overall procedure, target structures, and treatment approaches of US-guided pharmacopuncture and acupotomy for nerve entrapment in the upper extremities of the cubital and radial tunnel, posterior interosseous nerve, carpal tunnel, and Guyon's canal syndrome. We believe that the findings of our study will serve as a foundation for future clinical research, practice, and education on US-guided KM procedures. Further research involving US-guided interventions should specify target structures in three-dimension to delineate the treatment areas.

Women's Spatial-Temporal Entrapment in Access to Urban Opportunities by Child Age (자녀 연령별 여성의 도시기회 접근성의 시.공간적 구속성에 관한 연구)

  • Kim, Hyun-Mi
    • Journal of the Korean Geographical Society
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    • v.43 no.3
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    • pp.358-374
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    • 2008
  • This study examines whether and how ages of child affect accessibility experiences of women and men differently. Space-time accessibility measures based on Time-geographic framework with activity-travel diary datasets in Portland Metro, US were calculated using GIS-based geocomputation, and spatial-temporal patterns of accessibility of dual-earner couples by ages of their youngest child were compared. The results are as follows. (1) Although more women than men work part-time, which would render women more spatial-temporal autonomy, accessibility levels of women are not higher than men's. It implies that there exists another constraint placed on women which largely stems from gender inequality. (2) It is distinctively women with child under age 6 of which accessibility spaces are found to be restricted doser to home compared to men. Women with no child or with child aged over 6, however, show more or less similar spatial-temporal patterns of accessibility with men's which are quite unvarying regardless of parental status and their child age. Women's accessibility experiences characterized by spatial-temporal entrapment, thus, can be seen as problems associated with gender rather than sex. (3) Intensified spatial-temporal entrapment of women with young child are associated with the significant spatial pegs shaping their accessibility spaces, which are located much closer to home compared to men's: workplaces and child's daycare centers.

Neuralgic Amyotrophy Manifesting as Mimicking Posterior Interosseous Nerve Palsy

  • Yang, Jin Seo;Cho, Yong Jun;Kang, Suk Hyung;Choi, Eun Hi
    • Journal of Korean Neurosurgical Society
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    • v.58 no.5
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    • pp.491-493
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    • 2015
  • The upper trunk of the brachial plexus is the most common area affected by neuralgic amyotrophy (NA), and paresis of the shoulder girdle muscle is the most prevalent manifestation. Posterior interosseous nerve palsy is a rare presentation in patients with NA. It results in dropped finger on the affected side and may be misdiagnosed as entrapment syndrome or compressive neuropathy. We report an unusual case of NA manifested as PIN palsy and suggest that knowledge of clinical NA phenotypes is crucial for early diagnosis of peripheral nerve palsies.

Preparation and Characteristics of Immobilized Sludge by the PAA Entrapment Method (PAA 포괄법에 의한 고정화 슬러지의 제조 및 특성에 관한 연구)

  • 최석순
    • Journal of Environmental Health Sciences
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    • v.28 no.3
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    • pp.49-54
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    • 2002
  • This study was conducted to evaluate the feasibility and characteristics of poly acrylamide (PAA) immobilized sludge as a microbial entrapment bead for wastewater treatment. In the PAA method of immobilized sludge, it was found that the optimum acrylamide concentration for actual wastewater treatment was to be 12%. When the sequencing batch reactor (SBR) was operated during 30 days, removal efficiencies of TOC and phosphate was 95% and 70ft, respectively. From this research, repeated cycle of anaerobic and aerobic conditions is required to enhance the removal of TOC and phosphate. During the operation, immobilized cells could be used without being disrupted.