• Title/Summary/Keyword: enrichment culture

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Biodegradation of Bunker-C Oil by the Mixed Enrichment Culture of Marine Bacteria (혼합배양 해양세균에 의한 Bunker-C유의 생물분해)

  • PARK In-Sick;PARK Jung-Youn;SUH Kuen-Hack;HONG Yong-Ki
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.20 no.2
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    • pp.152-156
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    • 1987
  • A mixed population of marine bacteria was obtained to catalize the biodegradation of bunker-C fuel oil by means of the enrichment culture technique. Samples used for the enrichment culture were collected from sea water and sediments in the vicinity of Pusan, Chungmu, and Ulsan in Korea. As the biodegradation of bunker-C oil proceeded, the number of bacteria increased from $1.1\times10^6\;to\;8.7\times10^8$ cells per ml when pH was bufferized by 0.1 M Tris-HCl buffer to 7.6, then oil dispersion increased to $OD^{540}$ 2.2 and approximately $48\%$ of the oil was biodegradated in 10 days. Oil dispersion was absolutely dependent on the addition of nitrogen and phosphate sources in sea water. High and low sulfur-containing bunker-C and crude oil could be dispersed similarly. Bunker-C oil was dispersed rapidly at the pH ranging from 7.0 to 8.0 and dispersed to the amount of 7.5 g per liter of sea water medium.

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Work-Familiy Interface and Organizational Outcomes in Female Managers: The Moderating Effects of Family-friendly Organizational Culture (여성관리자의 일-가정 상호관계와 조직성과: 가족친화적 조직문화의 조절효과 분석)

  • Kim, Eun-Ju;Han, In-Soo
    • The Journal of the Korea Contents Association
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    • v.15 no.10
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    • pp.436-446
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    • 2015
  • Work-family interface including work-family conflict and work-family enrichment has an effect on organizational performance such as job satisfaction and organizational commitment. This empirical research mainly investigated influence of family-friendly organizational culture on work-family interface and organizational performance. Three key variables apply for this research : work-family interface, organizational performance and family-friendly organizational culture. A total 750 questionnaire was collected from female managers in various companies for hypothesis testing. The empirical analysis shows that reducing work-family conflict and enhancing work-family enrichment positively contribute to job satisfaction and organizational commitment. The Family-friendly organizational culture plays a moderating role between work-family interface and organizational performance. As a result, the findings support the fact that family-friendly organizational culture impact on organizational performance through work-family balance.

Recovery and Survival of Listeria monocytogenes in Surface and Sea Water (지표수 및 해수로부터 Listeria monocytogenes의 분리 및 생존성)

  • Yang, Ju;Kim, Toh-Gyong;Kang, Ho-Jo
    • Korean Journal of Veterinary Research
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    • v.42 no.3
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    • pp.327-333
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    • 2002
  • The study was carried out to examine the distribution and survival rate of Listeria monocytogenes (L monocytogenes) from various source of waters using improved isolation method. In comparision of enrichment media for isolation of L monocytogenes from water, the isolation rate and 50% detection limit of the pathogen were higher in UVM modified Listeria enrichment broth (UVM) than Listeria enrichment broth (LEB). On the other hand, when compared the selective media for isolation of the pathogen from water, the isolation rate was highest in culture at Oxford agar followed by Fraser agar, and LEB agar. In order to improve enrichment method, 100 ml of water samples with 0.1 CFU/ml of L monocytogenes was inoculated into 10 ml of UVM concentrated at 10-fold, and incubated for 24 h at $36^{\circ}C$. Isolated frequency of the pathogens in improved enrichment method completely corresponded with common (filter) method. Of a total mumber of 147 water samples from river, lake and sea, the pathogen was isolated from 1 of 39 (2.6%) river water samples and 1 of 75 (1.3%) sea water samples, but no pathogen was isolated from 33 lake water samples. Serotypes of 2 isolates were identified as type 1. L monocytogenes decreased in number from 7.2-7.4 to 4.2-4.7 log CFU/ml for 1 week poststorage (5 and $20^{\circ}C$), but the pathogens were able to be detected in river and sea water until 8 weeks after storage. However, in tap water, L monocytogenes were decreased to undetectable level after 2 weeks of storage.

Organic Enrichment and Pollution in Surface Sediments from Shellfish Farming in Yeoja Bay and Gangjin Bay, Korea (패류양식어장 밀집해역의 퇴적환경내 유기물 분포특성 -여자만과 남해 강진만-)

  • Choi, Minkyu;Kim, Hyung Chul;Hwang, Dong-Woon;Lee, In-Seok;Kim, Young-Sook;Kim, Ye-Jung;Choi, Hee-Gu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.46 no.4
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    • pp.424-436
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    • 2013
  • Organic enrichment was investigated in surface sediments from the Yeoja and Gangjin Bays of Korea, which contain dense shellfish farms, in order to evaluate the contamination status and temporal changes in shellfish farming along these coasts. The degree of organic enrichment was determined using geochemical indicators (chemical oxygen demand, ignition loss, acid volatile sulfide, total organic carbon in sediments, and total nitrogen in sediments, ammonium and nitrate in pore water, and bioluminescence inhabitation for sediments). Temporal changes in organic enrichment conditions were detected by comparing our 2012 data to those previously reported from a survey conducted in 1999/2000. Organic enrichment was significantly higher in September than in May and July, in Gangjin Bay than in Yeoja Bay, and significantly higher in shellfish farms than in reference sites not used to culture shellfish. Ammonium concentrations in pore water were two orders of magnitude greater than nitrate concentrations, suggesting that these bays represent nitrogen-reducing environments.

Food value or Freshwater Rotifer (Brachionus calyciflorus) for Culture of Sweetfish (Plecoglossus altivelis) Larvae (은어 자어 (Plecoglossus altivelis) 사육에 있어서 담수산 rotifer (Brachionus calyciflorus)의 먹이효과)

  • LEE Kyun Woo;PARK Heum Gi;LEE Sang-Min;HAN Hyon Sob;LIM Young Soo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.37 no.1
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    • pp.7-12
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    • 2004
  • This study investigated the possibility of salinity acclimation of freshwater rotifers (Brachionus calyciflorus) as live food for sweetfish (Plecoglossus altivelis) larvae, and also examined the optimal salinity for the growth of sweetfish. Freshwater rotifers cultured in 0 and 4 PSU and seawater rotifers (B. rotundiformis) cultured in 33 PSU were supplied to the larvae with four kinds of enrichment material (condensed freshwater Chlorella, $\omega-yeast,$ baker's yeast, Super Selco) and larval growth at 4 PSU was examined. Growth of the freshwater rotifers positively increased from 0 PSU to 6 PSU, but decreased when over 8 PSU was reached. Growth and survival of the sweet fish larvae reared in 0 PSU were significantly lower than those reared in either 4 PSU or 33 PSU. This indicated that the freshwater rotifers (B. calyciflorus) could be used as live food for sweetfish larvae reared in 4 PSU. The body weight of sweetfish larvae fed on freshwater rotifers enriched with Super Selco was the highest at 0.163 mg, but there was no significant difference in survival and body length of the fish fed with the other enrichment materials. The content of n-3 HUFA of the sweetfish larvae fed on the freshwater rotifers enriched with Super Selco and the condensed freshwater Chlorella was higher than that enriched with $\omega-yeast$ and baker's yeast. These results indicated that B. calyciflorus cultured with the condensed freshwater Chlorella could be used for the sweetfish larvae without enrichment, and the most efficient enrichment material for B. calyciflorus is Super Selco.

EFFECTS OF TRANSFORMATION CAPACITY ON COMETABOLIC DEGRADATION OF TRICHLOROETHENE

  • Lee, Seung-Bong;Kim, Geon-Ha
    • Environmental Engineering Research
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    • v.10 no.2
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    • pp.79-87
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    • 2005
  • The effects of transformation capacity on cometabolic degradation of trichloroethene (TCE) were evaluated using TCE-degrading actinomycetes pure and mixed culture under various culture conditions. The TCE transformation capacity of the actinomycetes enrichment culture in a batch test with phenol addition was 1.0 mg of TCE/mg of volatile suspended solids (VSS). The resting cell TCE transformation capacity of the actinomycetes pure culture cell was 0.75 mg TCE/mg VSS, which increased to 2.0 mg TCE/mg VSS when phenol was added as an external substrate. When the pure culture had an internal substrate in the form of poly-β-hydroxybutyrate (PHB) at 19% of the cell mass, the resting cell TCE transformation capacity increased from 0.47 to 0.6 mg TCE/mg VSS. The presence of PHB increased transformation capacity by 57%, whereas, the addition of phenol caused more than two fold increase in transformation capacity. The actinomycetes culture showed the highest transformation capacity.

The Optimal Enrichment Condition of Rotifer Brachionus rotundiformis (소화효소 활성으로 본 rotifer Brachionus rotundiformis의 적정 영양강화 조건)

  • Kwon, O-Nam;Park, Heum-Gi
    • Journal of Aquaculture
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    • v.21 no.1
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    • pp.41-46
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    • 2008
  • The purpose of the study was to suggest the optimal lipid enrichment conditions used digestive enzyme activity of rotifer changing due to water temperature and salinity. The high population growth appeared at the experiment temperature more than 28 degrees highly on the culture temperature(maximum 32 degrees, 1,453 individual/mL). The fecundity was low at high temperature, and the egg ratio was high at low temperature. Population growth of 10 and 15 ppt appeared in most highly, but the fecundity and the egg ratio were high most significantly appeared in natural seawater(32 psu). The digestive enzyme activity by the culture environment mainly showed high activity in natural seawater(amylase exclusion, 15 psu). However, the TAP activity by the water temperature showed highly at the more high temperature, but the amylase and the lipase appeared at low temperature. We carried out the lipid enrichment at 20 degrees and 26 degrees in a condition of the natural seawater. Total protein, the total essential amino acids differed not significantly. The methionine content that was essential amino acids, a total lipid content, unsaturated index of fatty acids, DHA and the DHA/EPA ratio were high significantly each in $20^{\circ}C$ enrichment trial. Therefore, we could suggest the $20^{\circ}C$ and natural seawater for the optimal lipid enrichment condition in aquaculture, because methionine contents, several indexes by the lipid, TG-lipase activity, fecundity and egg ratio are high.