• 한국농업기계학회:학술대회논문집
• /
• 한국농업기계학회 2003년도 하계 학술대회 논문집
• /
• pp.402-406
• /
• 2003

묘생산 시스템은 크게 개방형과 폐쇄형으로 구분된다. 개방형 묘생산 시스템은 시스템 내부와 외부의 공기ㆍ물ㆍ열 등의 교환이 가능한 시스템을 의미한다. 현재 실생묘, 삽목묘, 접목묘 등은 일사가 투과되는 온실 또는 묘포장에서 생산된다. 그러므로 온실 또는 묘포장을 이용한 묘생산 시스템은 개방형 묘생산 시스템에 해당한다. 개방형 묘생산 시스템에서는 외부기상의 변동에 따라 시스템 내의 기온, 상대습도, 광량, 기류속도, $CO_2$ 농도 등의 물리적 환경요인이 변화하므로 이들 환경 요인을 일정한 수준으로 제어하기가 쉽지 않다. 때문에 균일한 묘소질을 지닌 묘의 계획적 생산이 근본적으로 불가능하다. (중략)

• Journal of Microbiology and Biotechnology
• /
• 제16권7호
• /
• pp.1149-1153
• /
• 2006

In this study, nuclease-resistant RNA aptamers that are specific for Jurkat T leukemia cells were selected by a subtractive systemic evolution of ligands by exponential enrichment (SELEX) method. A randomized nuclease-resistant RNA library was incubated with normal peripheral blood mononuclear cells (PBMC) in each round to preclude RNAs that recognize the common cellular components on the surface of normal and cancer cells. The precluded RNAs were used for the selection of Jurkat T cell-specific aptamers, and the specific RNAs were then gradually enriched from start to the following selections. After 16 rounds of the subtractive SELEX, the selected aptamers were found to preferentially bind to Jurkat T cells, but not to the normal PBMC, evidenced by fluorescence-activated cell sorting analysis. Thus, the subtractive SELEX can be used to identify ligands to cancer-specific biological markers without prior knowledge of the nature of markers. The aptamers could be applied to specific cell sorting, tumor therapy, and diagnosis, and moreover, to find cancer cell-specific markers.

• Journal of Microbiology and Biotechnology
• /
• 제11권2호
• /
• pp.302-309
• /
• 2001

Colony blot hybridization using a VVHP DNA probe derived from the sequence of the hemolysin gene, vvhA, was specific in identifying all V. vulnificus strains, thereby, eliminating the need for any additional phenotypic identification. The colony blot hybridization procedure revealed a sensitivity and broad applicability sufficient for the direct enumeration of V. vulnificus in various natural samples, without the use of enrichment or culturing on selective medis. V. vulnificus was detected in all natural samples collected during August and May at concentrations ranging from $2.1{\times}10^1\;to\;4.0{\times}10^3$ organisms per ml. However, during November and February, when the mean temperatures of the seawater were $12^{\circ}C$ and $5^{\circ}C$, respectively, V. vulnificus was not detected in any natural samples.

• Journal of Microbiology and Biotechnology
• /
• 제13권4호
• /
• pp.636-639
• /
• 2003

Entercbacter agglomerans 6L was isolated from citron (Citrus junos) peel by using an enrichment culture containing (+)-limonene. It was able to metabolize limonene and grew well ($A_{600}$:4.5) on limonene as a sole carbon source. E. agglomernas 6L was highly resistant to limonene toxicity, and grew to 1.0 optical density ($A_{600}$) even at 5% (v/v) of limonene in Luria-Bertani media. ${\gamma}-Valerolactone$ and cryptone were detected as the major metabolic products of limonene by E. agglomerans 6L.

• 한국도서관정보학회지
• /
• 제16권
• /
• pp.221-249
• /
• 1989

The purpose of this study is to analyze the factors influencing on the organizational changes of the university libraries. Results of the analysis, that is, factors that cause the library organization to change, are as follows 1. Changes in library size physical facilities, growth and diversity of library collections, change in personnel organization, role enrichment and faculty status for librarians, management pattern of library director, change in financial su n.0, pport. 2. Change in program : change in goals and objectives, expansion of service programs, enhancement of library functions. 3. Library automation : introduction and development of many library automation projects. 4. Introduction of information technology : development and distribution of computer and related software, telecommunication, and new media. 5. Changes in user needs : many-sided demands for collections, spaces, and services. 6. Many other environmental factors related to library organization.

• Ocean and Polar Research
• /
• 제25권1호
• /
• pp.1-7
• /
• 2003

The purpose of this study was to determine changes in the growth rates and the degree of shell bioerosion exhibited by endolithic organisms of the Japanese scallop family, Patinopecten (Mizuhopecten) yessoensis, on the coast of Furugelm Island (Peter the Great Bay, northwest of East Sea = Sea of Japan) over the last three decades. The areas studied are affected by lumen (Tumangang) River run-off, which is enriched by organic matter and polluting agents. It was found that the linear growth rates of the Japanese scallops living along the coasts of Furugelm Island have decreased over the last three decades. The degree of bioerosion of scallop shells has significantly increased for the same period. These phenomena may be explained by a gradual increase in bottom sediment silting, organic enrichment and pollution of the areas being studied. It was found that the degree of scallop shell bioerosion increased with the scallop's age. At present, In each age group, the shells of the scallops sampled from the muddy sand showed greater erosion than the shells of individuals collected from the sandy substrate.

• Journal of Microbiology and Biotechnology
• /
• 제19권7호
• /
• pp.647-650
• /
• 2009

Using 200 porcine colon tissues, the efficiencies of three isolation methods of Campylobacter from porcine intestines were compared: Method 1, direct streaking of colon mucosa; Method 2, direct inoculation of intestinal contents with a swab; Method 3, inoculation of pre-enriched medium. A total of 460 Campylobacter isolates were obtained from 178 samples (89%) by direct streaking of colon mucosa, 142 samples (71%) by direct streaking of a swab, and 94 samples (47%) by pre-enrichment of intestinal contents in Preston broth. Direct streaking of colon mucosa was superior to the other two isolation methods, in terms of rapidity and higher efficiency. When isolates were identified with various biochemical tests and PCRs specific to 16s rRNA, mapA, and ceuE, C. coli was the predominant species (87%) in porcine, whereas the rest of the isolates were identified as C. lanienae.

• 환경위생공학
• /
• 제13권2호
• /
• pp.34-39
• /
• 1998

For more convenient and rapidly isolation of Bacillus thuringiensis subsp. israelensis(Bti), 1) heat treatment spore forming bacteria, 2) growth in enrichment culture media for Bacillus sp. and 3) selection of bacteria producing a lecithinase for Bacillus thuringiensis subsp. israelensis, were performed. Spore forming bacteria were counted 4.8 $\times $ 10$^{8}$cells/g soil on NAPGCY media, 9.2 $\times $ 10$^{7}$cells/g soil on NA media, and 3.6 $\times $ 10$^{8}$cells/g soil on NAAC media, respectively. Bacteria producing only a lecithinase were reached at 25.2% on medium contained egg york, bacteria only producing a delta-endotoxin were reached at 23.2% by phase contrast microscope, and bacteria producing a lecithinase & a delta-endotoxin simultaneously were reached at 13.7%. Bacillus thuringiensis which producing a lecithinase and a delta-endotoxin simultaneously among bacteria producing a lecithinase, were reached at 56.5%; A half of Bacillus thuringiensis was produced a delta-endotoxin, but not produced a lecithinase. Among 8 isolates of Bacillus thuringiensis, two strain of Bti which has a mosquito-cidal toxin, were detected by PCR using a specific primer of $\delta $-endotoxin gene from Bti.

• 한국원자력학회:학술대회논문집
• /
• 한국원자력학회 1996년도 춘계학술발표회논문집(3)
• /
• pp.347-352
• /
• 1996

Studies on tile microstructural and compositional changes in sensitized Ni base Alloy 600 by laser surface melting have been carried out using TEM equipped with EDXA. The microstructure of the laser melted zone was mainly consisted of fine cells, and along the cell and grain boundaries, Cr enrichment due to its segregation was observed. Cr carbides having formed along the grain bundaries during the sensitization treatment have been completely dissloved. The cell walls were decorated with dislocations and the very tiny precipitates, found to be Ti(CN) type, were distributed randomly along the cell walls with tangled dislocations around them.

• 한국진공학회지
• /
• 제5권2호
• /
• pp.107-112
• /
• 1996

Thin films of $Pb(Zr_xTi_{1-x})O_3$)PZT) were grown on $Pt/SiO_2/Si(100)$ at various temperatures by RF magnetron sputtering method. Surface morphology of these films were studied by using Atomic Force Microscopy(AFM). These films were also analyzed by using Atomic Force Microscopy(AFM). These films were also analyzed by using X-ray photoemission spectroscopy(XPS) for determining their chemical composition and their depth profile. It was found that the films grown at the substrated temperature of $300^{\circ}C$ have much more smooth surface characteristics in comparison to those films grown at room temperature, which may be explained in terms of surface mobility of ad-atoms such as Pb. It was also found that Pb enrichment in the near surface region enhanced for the films grown at higher substate temperature.