• Title/Summary/Keyword: enrichment

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Biodegradation of PCBs into Contaminated Dredged Sediments (오염퇴적물에 함유된 PCBs의 생물분해 특성)

  • 안재환;김소정;지재성;배우근;황병기
    • Journal of Soil and Groundwater Environment
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    • v.9 no.2
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    • pp.41-47
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    • 2004
  • This research in the degradation of polychlorinated biphenyls(PCB) has focussed on the use of experimental enrichment cultures to obtain PCB-deading communities and identification of PCB-degrading bacteria accor야ng to pure culture. During 180 days, enrichment culture was performed to obtain PCB-degrading bacteria and initial concentration was injected 1.6 ppm,0.7 ppm, respectively. After 180 days of enrichment culture, PCBs was removed 80-87% and 57-71%. Biodegradation of PCBs was studied according to dominated PCB-degrading bacteria. Biodegraddation of PCBs was 80% in initial concentration of PCBs for 20days, enrichment cultured PCB-degrading bacteria was isolated by pure culture and it was verified to Pseudoxanthomonas sp.

Enrichment Strategies for Identification and Characterization of Phosphoproteome

  • Lee, Sun Young;Kang, Dukjin;Hong, Jongki
    • Mass Spectrometry Letters
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    • v.6 no.2
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    • pp.31-37
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    • 2015
  • Phosphorylation upon protein is well known to a key regulator that implicates in modulating many cellular processes like growth, migration, and differentiation. Up to date, grafting of multidimensional separation techniques onto advanced mass spectrometry (MS) has emerged as a promising tool for figuring out the biological functions of phosphorylation in a cell. However, advanced MS-based phosphoproteomics is still challenging, due to its intrinsic issues, i.e., low stoichiometry, less susceptibility in positive ion mode, and low abundance in biological sample. To overcome these bottlenecks, diverse techniques (e.g., SCX, HILIC, ERLIC, IMAC, TiO2, etc.) are continuously developed for on-/off-line enrichment of phosphorylated protein (or peptide) from biological samples, thereby helping qualitative/quantitative determination of phosphorylated protein and its phosphorylated sites. In this review, we introduce to the overall views of enrichment tools that are universally used to selectively isolate targeted phosphorylated protein (or peptide) from ordinary ones before MS-based phospoproteomic analysis.

The Flame Structure of $CH_4/O_2/N_2$ Premixed Flames on the $O_2$ Enrichment ($CH_4/O_2/N_2$ 예혼합화염에서 산소부화에 따른 화염구조)

  • Lee, Ki-Yong;Nam, Tae-Hyoung;You, Hyun-Seok;Choi, Dong-Soo
    • 한국연소학회:학술대회논문집
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    • 2001.06a
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    • pp.53-59
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    • 2001
  • Experimental measurements are conducted to investigate the structure of flat $CH_4/O_2/N_2$ premixed flames. The flames are simulated using a detailed chemical kinetic mechanism. Four flames established at equivalence ratio = 0.55 are studied with the different $O_2$ enrichment level, ${\Omega}$ = 0.21, 0.25, 0.30, and 0.35. The measured flame speed and species composition profiles are compared with the calculations. Whereas there is overall good agreement between the measurements and predictions, it appears that as the $O_2$ enrichment level is increased the position of the flame is moved toward the exit of the burner and the rapid temperature rise happens near the exit of the burner, and some areas of further refinement in the kinetic mechanism are identified.

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The Investigation of CF4 Decomposition in Methane Premixed Flames on Oxygen Enrichment (산소부화된 메탄 예혼합 화염에서 CF4 분해에 대한 연구)

  • Lee, Ki Yong
    • Journal of the Korean Society of Combustion
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    • v.22 no.4
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    • pp.51-56
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    • 2017
  • The decomposition of tetrafluoromethane has been investigated with the reaction mechanism proposed for freely propagating $CH_4/CF_4/O_2/N_2$ premixed flames on the oxygen enrichment. The factors affecting on the removal efficiency of tetrafluoromethane were analyzed. The increase in flame temperature due to oxygen enrichment has a great influence on the removal efficiency of tetrafluoromethane. At the same oxygen enrichment condition, the removal efficiency in the rich flame is higher than one in the lean flame. The increase of the F/H ratio leads to decrease the flame temperature and the removal efficiency of tetrafluoromethan is decreased at the flame temperature of 2600 K or lower, The elementary reactions that dominate the consumption of tetrafluoromethane are (R1) $CF_4+M=CF_3+F+M$ and (R2) $CF_4+H=CF_3+HF$. (R1) has the greatest effect on the consumption of tetrafluoromethane under the oxygen enhanced flames.

Effect of Oxygen Enrichment in a Swirling Diffusion Gas Burner (선회 확산버너에서 산소부화가 연소장에 미치는 영향)

  • Lee, Yong-Hoo;Lee, Jin-Seok;Lee, Woo-Seob;Lee, Do-Hyung
    • Journal of the Korean Society of Combustion
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    • v.7 no.2
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    • pp.34-41
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    • 2002
  • To investigate the combustion characteristics of a swirling diffusion gas burner with oxygen enrichment, mean temperature, CO, $CO_2$, and HC concentrations were measured at various oxygen enrichment conditions. According to the results, the flame temperature increased and the region of high temperature was expanded with increasing oxygen concentration. The $CO_2$ concentrations increased, while the CO concentrations decreased in proportion to the increase of oxygen concentration. On the other hand, the HC concentrations were decreased and this tendency was very strong at the downstream of the combustor.

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An Experimental Study on the Combustion Characteristics with Hydrogen Enrichment in a Dump Combustor (수소 혼합에 따른 덤프 연소기내의 연소 특성에 관한 실험적 연구)

  • Kim, Dae-Hee;Hong, Jung-Goo;Shin, Hyun-Dong
    • Proceedings of the KSME Conference
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    • 2008.11b
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    • pp.2977-2983
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    • 2008
  • The combustion characteristics of a partially premixed flame in a dump combustor were studied to determine the effects of hydrogen enrichment in propane. Bluff-body was used for flame stabilization. Fuel mixtures containing a hydrogen mole fraction ranging from 0.1 to 0.5 were burnt at ambient pressure within a quartz chamber. Tests were carried out keeping the total reactant flow rate by adjusting the fuel and air flow rates. The fluctuations of pressure were measured by piezoelectric pressure sensor. The instantaneous flame structure and OH chemiluminescence images were described by High-speed Intensified Charged Coupled Device (HICCD) camera and Intensified Charged Coupled Device (ICCD) camera. The present results show that hydrogen enrichment in fuel changed the location of primary reaction zone from inner recirculation zone to turbulent shear layer and pressure signal. The reason is that chemical aspects take precedence over flow aspects in the hydrogen-enriched flame.

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Towards improving finite element solutions automatically with enriched 2D solid elements

  • Lee, Chaemin;Kim, San
    • Structural Engineering and Mechanics
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    • v.76 no.3
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    • pp.379-393
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    • 2020
  • In this paper, we propose an automatic procedure to improve the accuracy of finite element solutions using enriched 2D solid finite elements (4-node quadrilateral and 3-node triangular elements). The enriched elements can improve solution accuracy without mesh refinement by adding cover functions to the displacement interpolation of the standard elements. The enrichment scheme is more effective when used adaptively for areas with insufficient accuracy rather than the entire model. For given meshes, an error for each node is estimated, and then proper degrees of cover functions are applied to the selected nodes. A new error estimation method and cover function selection scheme are devised for the proposed adaptive enrichment scheme. Herein, we demonstrate the proposed enrichment scheme through several 2D problems.

Rapid Detection of Escherichia coli in Fresh Foods Using a Combination of Enrichment and PCR Analysis

  • Choi, Yukyung;Lee, Sujung;Lee, Heeyoung;Lee, Soomin;Kim, Sejeong;Lee, Jeeyeon;Ha, Jimyeong;Oh, Hyemin;Lee, Yewon;Kim, Yujin;Yoon, Yohan
    • Food Science of Animal Resources
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    • v.38 no.4
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    • pp.829-834
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    • 2018
  • The objective of this study was to determine the minimum enrichment time for different types of food matrix (pork, beef, and fresh-cut lettuce) in an effort to improve Escherichia coli detection efficiency. Fresh pork (20 g), beef (20 g), and fresh-cut lettuce (20 g) were inoculated at 1, 2, and 3 Log CFU/g of Escherichia coli. Samples were enriched in filter bags for 3 or 5 h at $44.5^{\circ}C$, depending on sample type. E. coli cell counts in the samples were enriched in E. coli (EC) broth at 3 or 5 h. One milliliter of the enriched culture medium was used for DNA extraction, and PCR assays were performed using primers specific for uidA gene. To detect E. coli (uidA) in the samples, a 3-4 Log CFU/mL cell concentration was required. However, E. coli was detected at 1 Log CFU/g in fresh pork, beef, and fresh-cut lettuce after 5, 5, and 3-h enrichment, respectively. In conclusion, 5-h enrichment for fresh meats and 3-h enrichment for fresh-cut lettuce in EC broth at $44.5^{\circ}C$, and PCR analysis using uidA gene-specific primers were appropriate to detect E. coli rapidly in food samples.

Effect of CO$_{2}$Enrichment on Growth of two Poplar Clones, I-214 (Populus euramericana) and Peace (P. koreana $\times$ P. trichocarpa) (환경조건에 대해 기공의 반응이 상이한 두 종의 포플라 생장에 미치는 고농도 CO$_{2}$의 영향)

  • Park, shin-Young;Akio Furukawa;Tsumugu Totsuka
    • The Korean Journal of Ecology
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    • v.18 no.2
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    • pp.255-263
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    • 1995
  • Two poplar clones, I-214 (Populus euramericana) and Peace (P. Koreana × P. trichocarpa), were grown for 21 days in growth chambers at different CO₂concentrations (350, 700 and 2,000 μL·L-1). I-214 has stomata responding to environmental conditions in normal ways and Peace has unresponsive stomata to environmental factors including light, ABA, water stress and CO₂. In both plants, elevated CO₂ stmulated the growth of plant parts, especially leaf dry weight. And a CO₂ enrichment of 700 μL·L-1 CO₂ caused increment of net assimilation rate (NAR). The growth responses of these plants to CO₂ enrichment were different especially at high CO₂ condition (2,000 μL·L-1 CO₂). The total dry weight in Peace increased up to 2,000 μL·L-1 CO₂ but not in I-214. A CO₂ enrichment of 2,000 2,000 μL·L-1 CO₂ had little effect on NAR of I-214 but enhanced NAR of Peace. Although it is uncertain whether the different responses to CO₂ enrichment between I-214 and Peace resulted from the different properties of stomatal responses to long-term CO₂ treatment, the decrease in NAR is probably due in part to CO₂-induced stomatal closure in I-214 but not in Peace.

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