• Title/Summary/Keyword: enhancer

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An OTHBVS Cell Line Expresses the Human HBV Middle S Protein

  • Park, Sung-Gyoo;Guhung Jung
    • Journal of Microbiology
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    • v.37 no.2
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    • pp.86-89
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    • 1999
  • An OTHBVS cell line from HepG2 was established. This cell line stably expresses the human hepatitis B virus (HBV) middle S protein that includes the preS2 region which is important for HBV particle entry into the hepatocyte. To establish this cell line, the middle S open reading frame (ORF), with a promoter located in the 5' region and enhancer located in the 3' region, was cloned downstream from the metallothionine (MT) promoter of the OT1529 vector. In this vector, expression of the middle S protein was constructed to be regulated by its own promoter and enhancer. Expression of the large S protein which contains the preS1 region in addition to the middle S protein was designed to be regulated by the MT promoter. When extracts of OTHBVS cells were examined with an S protein detection kit (RPHA, Korea Green Cross Co.), an S protein was detected. Total mRNA of OTHBVS cell examined by northern blot analysis with an S ORF probe revealed small/middle S transcripts (2.1 kb). When the MT promoter was induced by Zn, large S transcripts (2.4 kb) were detected. The GP36 and GP33 middle S proteins were presumably detected, but large S proteins were not detected by immunostain analysis using anti-preS2 antibody.

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Active noise control with on-line adaptive algorithm in a duct system (덕트에서 온라인 적응 알고리듬을 이용한 능동소음제어)

  • Kim, Heung-Seob;Hong, Jin-Seok;Oh, Jae-Eung
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.21 no.8
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    • pp.1332-1338
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    • 1997
  • In the case of the transfer function for the secondary path is dependent on time, the on-line method which can model it is continuously must be applied to the active noise control technique. And the adaptive random noise technique among the on-line methods is effective in the narrow-band control. In this method, the signal to noise ratio between random noise for modeling and primary noise is low. Therefore, the estimations of transfer function will be prone to inaccuracies and the convergence time will be too long. Such imperfections will have an influence upon the performance of an active noise controller. In this study, t enhance the signal to noise ratio, the on-line method that is combined the conventional adaptive random noise technique and the adaptive line enhancer, is proposed. By using proposed on-line method, a rigorous system identification and control of primary noise have been implemented.

Formulation and In vitro Evaluation of Transdermal Drug Delivery System for Galantamine

  • Hossain, Md. Kamal;Subedi, Robhash Kusam;Chun, Myung-Kwan;Kim, Eun-Jung;Moon, Hwan-Shik;Choi, Hoo-Kyun
    • Journal of Pharmaceutical Investigation
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    • v.41 no.1
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    • pp.1-7
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    • 2011
  • The effects of different formulation variables including pressure sensitive adhesive (PSA), permeation enhancer, thickness of the matrix and loading amount of drug on the transdermal absorption of galantamine were investigated across the hairless mouse skin. The permeation profile of galantamine was different depending on the types of PSA, loading amount of drug, thickness of the matrix and type of enhancer used. Highest flux of galantamine was obtained from acrylic PSA but crystals were formed in the patch within 72 h. Among the PSAs screened, crystal formation was not observed only in the patches formulated in Styrene Butadiene Styrene (SBS) matrix. Permeation rate increased linearly as the concentration of galantamine in SBS matrix increased from 2.5 to 15% w/w. Among the enhancers screened, Brij$^{(R)}$ 30 provided highest flux of galantamine. Matrix thickness of 80 ${\mu}m$ was optimum for maintaining adhesiveness as well as consistently delivering galantamine for longer period of time.

A Study on Lubricant additive of DME Common-rail Vehicle (DME 커먼레일 차량의 윤활향상제에 관한 연구)

  • Park, JungKwon;Kim, Hyunchul;Jeong, SooJin;Chon, MunSoo
    • Journal of Institute of Convergence Technology
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    • v.3 no.1
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    • pp.15-18
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    • 2013
  • The next generation alternative fuel of diesel, DME (Dimethyl Ether) discharges particulate matter hardly due to chemical structural as oxygen-fuel so it has the eco-friendly property. Despite these advantages, the DME has the technical difficulties to apply to the diesel engine because of a low calorific value, viscosity and compressibility effects. From this point of view, we performed experimental studies on improved reliability of DME common-rail vehicle and lubricity enhancement of DME fuel for empirical distribution of eco-friendly DME fuel. Also we analyzed solubility of lubrication enhancer according to a drop in temperature, try to secure reliability about core parts of DME vehicle by applying lubrication enhancer in the DME common-rail vehicle.

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Active noise control with the active muffler in automotive exhaust system (액티브 머플러를 이용한 자동차 배기계의 능동소음제어)

  • Kim, Heung-Seob;Hong, Jin-Seok;Oh, Jae-Eung
    • Transactions of the Korean Society of Mechanical Engineers A
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    • v.21 no.11
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    • pp.1837-1843
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    • 1997
  • This study experimentally demonstrates the use of active muffler attached to the automotive exhaust system to reduce exhaust noise. For improving the signal to noise ratio in the process of estimation of secondary path transfer functions, the on-line algorithm that conventional inverse modeling is combined with adaptive line enhancer is used as the control algorithm. Active muffler is designed that the primary noise and the control sound are propagated as a plane wave in the outlet. Therefore, the error microphone could be placed out of the tail pipe center of a high temperature and the radiation noise to the outside could be reduced in the whole area around the outlet. The control experiment for reducing exhaust noise with active muffler is implemented during run-up at no load. From the experimental results presented, compared with the conventional off-line method, the proposed on-line method is capable to acquire a reduction of exhaust noise above 5 dB in overall sound power level.