• 제목/요약/키워드: engineered pattern

검색결과 22건 처리시간 0.024초

Growth Pattern and Content of Tropane Alkaloids of Metabolic Engineered Scopolia parviflora Hairy Root Lines

  • Kang, Young-Min;Min, Ji-Yun;Kim, Won-Jung;Kim, Yong-Duck;Lee, Byung-Hynn;Choi, Myung-Suk
    • 한국약용작물학회지
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    • 제12권2호
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    • pp.123-128
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    • 2004
  • Hyoscyamine and scopolamine are two most common tropane alkaloids found in the Solanaceae. The pEB expression vector carrying Nspmt gene was transformed to Agrobacterium rhizogenes. The growth of transgenic hairy roots were approximately to 80% of the wild type root. Transgenic hairy roots are less developed on only their branch roots than those of the wild type root. The extracts from Nspmt transgenic hairy root lines, 3 and 5 contained between 3.52 and 4.23 mg/g dry weight as hyoscyamine and did between 5.23 to 6.40 mg/g dry weight as scopolamine. These results showed that the overexpression of the pmt gene enhanced tropane alkaloids production of S. parviflora transformed roots and this improvement affected both hyoscyamine and scopolamine production.

Buckling analysis of noncontinuous linear and quadratic axially graded Euler beam subjected to axial span-load in the presence of shear layer

  • Heydari, Abbas
    • Advances in Computational Design
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    • 제5권4호
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    • pp.397-416
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    • 2020
  • Functionally graded material (FGM) illustrates a novel class of composites that consists of a graded pattern of material composition. FGM is engineered to have a continuously varying spatial composition profile. Current work focused on buckling analysis of beam made of stepwise linear and quadratic graded material in axial direction subjected to axial span-load with piecewise function and rested on shear layer based on classical beam theory. The various boundary and natural conditions including simply supported (S-S), pinned - clamped (P-C), axial hinge - pinned (AH-P), axial hinge - clamped (AH-C), pinned - shear hinge (P-SHH), pinned - shear force released (P-SHR), axial hinge - shear force released (AH-SHR) and axial hinge - shear hinge (AH-SHH) are considered. To the best of the author's knowledge, buckling behavior of this kind of Euler-Bernoulli beams has not been studied yet. The equilibrium differential equation is derived by minimizing total potential energy via variational calculus and solved analytically. The boundary conditions, natural conditions and deformation continuity at concentrated load insertion point are expressed in matrix form and nontrivial solution is employed to calculate first buckling loads and corresponding mode shapes. By increasing truncation order, the relative error reduction and convergence of solution are observed. Fast convergence and good compatibility with various conditions are advantages of the proposed method. A MATLAB code is provided in appendix to employ the numerical procedure based on proposed method.

자가연골세포와 PLA-coated PGA 복합체를 이용한 연골조직 재생 (Engineering Autogenous Cartilage Using PLA Coated PGA Chondrocyte Complex)

  • 김우섭
    • Archives of Plastic Surgery
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    • 제32권1호
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    • pp.1-4
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    • 2005
  • Previous sucessful results of neocartilage formation using tissue engineering technique in immunocompromised nude mouse xenograft model were reported. For clinical application, autogenous cell is preferrable to allogenic or xenogenic cell for circumvention of immune rejection. This study evaluates the feasibility of producing a engineered cartilage using autogenous chondrocytes. Chondrocytes were isolated from the auricular catilage of New Zealand White rabbit and seeded onto PGA polymer coated with polylactic acid in round pattern(diameter 0.7 cm, thickness 0.1 cm) at a concentration $7{\times}10^7$ chondrocytes per $cm^3$. Each Autogenous Cell-polymer constructs were implanted subcutaneously into the left side of dorsum of twelve Rabbits. Polymer templates not containg cells were implanted into the right side as a control. Fifteen rabbits were sacrificed at the following intervals: 5 rabbits at nine weeks, 7 rabbits at twelve weeksNew autogenous cartilage formation which retained the approximate dimensions of origianl round polymer template in 11 of 12 cell seeded implants. Histological examination using hematoxyline and eosin stain revealed vast majority of implants developed into mature cartilage. This study opens up the possibility of autologus cell transplant to construct autogenous cartilge.

페이즐리 오너먼트를 활용한 패션텍스타일 디자인의 현대적 특징 연구 (Contemporary Characteristics of Fashion-textile Design Applying Paisley Ornament)

  • 정하정;박주희
    • 한국의류학회지
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    • 제44권5호
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    • pp.950-968
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    • 2020
  • This study derives the modern characteristics of the paisley ornament through an analysis of the paisley ornament shown in fashion since the 2010s based on a theoretical review of the paisley ornament used in Kashmir India shawls for artistic inspiration in the fashion-textile field. It classified the paisley ornament in fashion since the 2010s into types of 'basic structure of body and coif', 'abnormal paisley ornament shapes', 'complex structure of paisley ornament', 'complex structure with other motif', 'a pair of symmetrical reflection motif', 'regular of repetitive arrangement', 'complex arrangement of irregularity and regularity' and 'free arrangement of irregularity'. A comparative analysis with the historical paisley ornament shows that paisley ornaments have contemporary characteristics like 'Bisector structure of centerline in criterion of the body', 'Composite structure of extended 1-repeat', and 'Free structure of engineered placement'. A modern design was inspired by the historical art forms; however, it was used in free utilization of motifs and patterns in terms of size, direction, proportion and space. These were the expression of a design identity that originated from the aesthetic ability and career of a designer as well as the technology of computer programs for the improvements in time and cost efficiency.

고인성 시멘트 복합재를 활용한 플랫플레이트 구조의 슬래브-기둥 접합부 구조성능 연구 (A Study on the Structural Performance of Slab-column Joint at Flat Plate Structure Using ECC)

  • 최광호;박병천;최성우;류득현
    • 콘크리트학회논문집
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    • 제29권2호
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    • pp.209-216
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    • 2017
  • 플랫플레이트 구조설계 시 중요한 고려사항 중 하나는 슬래브-기둥 접합부가 축하중에 의한 뚫림전단에 대한 저항성과 지진 발생 시 건물전체의 수평변형에 추종할 수 있는 연성능력을 확보하는 것이다. 이 연구에서는 플랫플레이트 구조형식의 슬래브-기둥 접합부를 대상으로 연성 증진을 위해 ECC를 접합부 뚫림전단의 위험단면 구역에 타설하고, 이 구역 주변 전단보강 구역에는 스터드의 설치와 강섬유 콘크리트를 사용한 상세를 개발하였으며, 이를 적용한 실험체에 대해 슬래브-기둥 접합부의 전단성능 실험을 수행하였다. 실험변수는 ECC에 혼입한 섬유 종류, 전단보강 구역의 스터드 설치와 강섬유 콘크리트 타설 여부였으며, 실험체의 파괴양상, 접합부 내력 및 변위와 변형률을 비교분석하였다. 실험 결과 슬래브-기둥 접합부에 ECC를 적용한 실험체의 내력과 연성이 그렇지 않은 실험체보다 우수하게 나타났으며, 전단보강 구역에서는 스터드의 전단보강 효과와 강섬유 콘크리트의 연성개선 효과를 확인할 수 있었다.

데이터 서비스를 위주로 하는 이동통신 망에서의 주파수 소요량 산출 모델 (The Model of Estimating Spectrum Requirements in Mobile Networks with Data Traffic)

  • 김대극;양은샘;김화종
    • 한국산학기술학회논문지
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    • 제10권2호
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    • pp.364-369
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    • 2009
  • WiMax, WiBro 등의 무선망에서의 데이터 서비스들은 더 빠른 이동성 확보와 더 높은 데이터 전송 속도의 실현을 목표로 변화와 발전을 계속하고 있다. 이러한 서비스 진화에서는 무선 데이터 통신망에서 가입자의 트래픽 수요를 충족시키고 무선망 구축을 위한 정확한 주파수 소요량 예측이 무엇보다 중요하다. 따라서 본 논문에서는 기존기술 연구의 문제점을 분석하여 데이터 서비스를 위주로 하는 이동 통신망의 주파수 소요량 산출시 고려해야 할 핵심 요소들을 제안하였다. 그것은 첫째 데이터 트래픽의 자기 유사성 특징, 둘째, QoS(Quality of Service)가 고려된 처리용량, 셋째, 기지국 부하율의 비균일 특성, 그리고 마지막으로 FA 증설구조이다. 또한 데이터 서비스를 위주로 하는 이동통신 망에서는 이러한 핵심 요소들을 적용하여 주파수 소요량 산출 표준 모델을 제안하였다.

주성분분석을 이용한 고속도로의 여가성 도로구간 판별에 관한 연구 (A Study on Characteristics of Highway Segments for Recreational Trips Using Principal Analysis)

  • 김영일;정진혁;금기정
    • 대한교통학회지
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    • 제22권2호
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    • pp.87-93
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    • 2004
  • 주5일 근무제가 본격적으로 시행됨에 따라 여가에 대한 관심이 증가함에 따라 기존에 행하지 못한 교외나 근거리, 원거리 통행이 비례적으로 증가함에 따라 관광지나 위락시설이 인접해있는 도로의 구간은 심한 정체가 발생될 것으로 예상된다. 이에 따른 문제를 고려할 때 이러한 여가 통행의 증가로 정체가 발생한 구간을 판별하고 분류하여 이에 대한 도로교통 운영 및 정비 등의 대응방안이 필요할 것으로 판단된다. 따라서 본 연구에서는 고속도로의 TCS자료의 구간단면 교통량을 이용하여 고속도로의 여가성 도로구간을 주성분분석을 통하여 판별하는 연구를 수행하여 여가성 도로와 비여가성 도로 통행특성 관계를 각 변수들의 특성을 규명하여 연구 수행의 근거를 제시하였다. 또 이러한 특성을 기반으로 변수들의 상관행렬을 이용한 주성분분석을 수행하여 주성분 점수를 산출하였다. 그 결과 고속도로의 총 197개의 구간 중 여가성 도로구간과 비여가성 도로구간을 추출하여 분류하고, 각 변수들의 비여가성 도로구간과 여가성 도로구간으로 분류할 수 있는 기준을 제시하였다.

위험 대상에 대한 충격 편향은 탈 편향 조작에 의해 감소하는가? (Does a Debiasing Manipulation Reduce Over-estimation of Emotional Reaction to Risky Objects?)

  • 윤지원;이영애
    • 인지과학
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    • 제22권1호
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    • pp.39-55
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    • 2011
  • 사람들은 이사, 투표, 시험, 부상, 차 구입과 같은 미래의 사건에 대한 자신의 정서 반응을 과도하게 예측한다. 충격 편향(impact bias)란 자신의 정서 반응이 강하게 오래 계속될 것으로 예상하는 것이다. 본 연구는 원자력 발전소, 유전자조작식품, 그리고 휴대폰과 같은 위험 대상에 대해 충격 편향이 관찰되는지, 그리고 관찰된다면 이 편향을 감소시키는 조작의 효과가 있는지를 검토하였다. 참여자들은 위험 대상에 대해서 현재보다 가까운 미래인 1주일 후 충격 편향을 가장 크게 보였다. 적응 과제와 탈초점 과제를 사용한 결과, 탈초점 조작이 위험 대상들에 대한 충격 편향을 감소시켰다. 본 연구의 결과들을 선행 연구들과 관련해서 논하였다.

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Genetically Engineered Yeast by Heterologous Transformation and Intergeneric Two-Step Protoplast Fusion for Ethanol Fermentation

  • Kim, Young-Ho;Lee, Jae-Ran;Seu, Jung-Hwn
    • Journal of Microbiology and Biotechnology
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    • 제3권4호
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    • pp.232-237
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    • 1993
  • A strain of yeast which can convert starch directly to ethanol was developed by the intergeneric protoplast fusion between Schwanniomyces alluvius possessing $\alpha$ amylase as well as glucoamylase with debranching activity and FSC-14-75 which previously had been formed from a heterologous transformation and subsequent intergeneric protoplast fusion. Fusants were selected on minimal medium after protoplasts of auxotrophic mutant of S. alluvius fused with heat-treated protoplasts of FSC-14-75 in the presence of 30%(w/v) PEG and 20 mM $CaCl_2$. The fusion frequency was in the range of $10^{-6}$ order. All fusants tested were intermediate types of parental strains for carbon compound assimilation, and their cell volumes were approximately 1.1 times larger than FSC-14-75 and 1.8 times larger than S. alluvius. The fusants were unable to sporulate like FSC-14-75, while S. alluvius could sporulate. In flask scale the most promising fusant, FSCSa-R10-6, produced 7.83%(v/v) and 10.17%(v/v) ethanol from 15% and 20% of liquefied potato starch, respectively, indicating that the fermetation efficiency of each case increased 1.2 times and 1.6 times than that of FSC-14-75. The elution pattern on DEAE-cellulose chromatography showed that FSCSa-R10-6 has four distinct amylase peaks of which two peaks originated from S. alluvius and the other two from FSC-14-75. These results suggest that the enhanced fermentation efficiency of the fusant might be due to almost-complemented parental amylases.

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3DP 폴리머-패브릭(3D Print Polymer-Fabric Structure)을 적용한 패션디자인 연구 (A Study of Fashion Design Applying a 3D Print Polymer-Fabric Structure)

  • 임소영;전재훈
    • 한국의류산업학회지
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    • 제25권2호
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    • pp.139-152
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    • 2023
  • Despite efforts to apply 3D print (3DP) technology in the field of fashion and endless discussions about the possibility of future development, in reality, it is difficult to utilize 3DP technology in fashion for reasons related to material, technology, and cost constraints. The purpose of this study was to supplement the limitations of 3DP technology in order to promote its utilization in fashion and simultaneously find a solution to achieve aesthetic satisfaction in the design method. Specifically, through the development of fashion products with a 3DP polymer-fabric structure to which the parametric design methodology has been applied, this study explored the possibility of practical application and proposes a new 3DP fashion design method. The 3DP polymer-fabric developed as a result of the research was stably adhered to the fabric. Additionally, the study confirmed the possibility of making 3DP clothes that are amenable to the wearer's activities, as it was verified that cutting and sewing tailored to the human body's curvature and structure can be performed. The design process using the 3DP polymer-fabric presented in this study is meaningful in that it suggests a solution to complement the limitations of modern technology in connection with designers' creativity. Moreover, the design process presented in this study is expected to contribute to the commercialization and generalization of 3DP by providing practical help to allow fashion experts to utilize 3DP technology.