• Title/Summary/Keyword: engineered pattern

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Growth Pattern and Content of Tropane Alkaloids of Metabolic Engineered Scopolia parviflora Hairy Root Lines

  • Kang, Young-Min;Min, Ji-Yun;Kim, Won-Jung;Kim, Yong-Duck;Lee, Byung-Hynn;Choi, Myung-Suk
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.2
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    • pp.123-128
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    • 2004
  • Hyoscyamine and scopolamine are two most common tropane alkaloids found in the Solanaceae. The pEB expression vector carrying Nspmt gene was transformed to Agrobacterium rhizogenes. The growth of transgenic hairy roots were approximately to 80% of the wild type root. Transgenic hairy roots are less developed on only their branch roots than those of the wild type root. The extracts from Nspmt transgenic hairy root lines, 3 and 5 contained between 3.52 and 4.23 mg/g dry weight as hyoscyamine and did between 5.23 to 6.40 mg/g dry weight as scopolamine. These results showed that the overexpression of the pmt gene enhanced tropane alkaloids production of S. parviflora transformed roots and this improvement affected both hyoscyamine and scopolamine production.

Buckling analysis of noncontinuous linear and quadratic axially graded Euler beam subjected to axial span-load in the presence of shear layer

  • Heydari, Abbas
    • Advances in Computational Design
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    • v.5 no.4
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    • pp.397-416
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    • 2020
  • Functionally graded material (FGM) illustrates a novel class of composites that consists of a graded pattern of material composition. FGM is engineered to have a continuously varying spatial composition profile. Current work focused on buckling analysis of beam made of stepwise linear and quadratic graded material in axial direction subjected to axial span-load with piecewise function and rested on shear layer based on classical beam theory. The various boundary and natural conditions including simply supported (S-S), pinned - clamped (P-C), axial hinge - pinned (AH-P), axial hinge - clamped (AH-C), pinned - shear hinge (P-SHH), pinned - shear force released (P-SHR), axial hinge - shear force released (AH-SHR) and axial hinge - shear hinge (AH-SHH) are considered. To the best of the author's knowledge, buckling behavior of this kind of Euler-Bernoulli beams has not been studied yet. The equilibrium differential equation is derived by minimizing total potential energy via variational calculus and solved analytically. The boundary conditions, natural conditions and deformation continuity at concentrated load insertion point are expressed in matrix form and nontrivial solution is employed to calculate first buckling loads and corresponding mode shapes. By increasing truncation order, the relative error reduction and convergence of solution are observed. Fast convergence and good compatibility with various conditions are advantages of the proposed method. A MATLAB code is provided in appendix to employ the numerical procedure based on proposed method.

Engineering Autogenous Cartilage Using PLA Coated PGA Chondrocyte Complex (자가연골세포와 PLA-coated PGA 복합체를 이용한 연골조직 재생)

  • Kim, Woo Seob
    • Archives of Plastic Surgery
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    • v.32 no.1
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    • pp.1-4
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    • 2005
  • Previous sucessful results of neocartilage formation using tissue engineering technique in immunocompromised nude mouse xenograft model were reported. For clinical application, autogenous cell is preferrable to allogenic or xenogenic cell for circumvention of immune rejection. This study evaluates the feasibility of producing a engineered cartilage using autogenous chondrocytes. Chondrocytes were isolated from the auricular catilage of New Zealand White rabbit and seeded onto PGA polymer coated with polylactic acid in round pattern(diameter 0.7 cm, thickness 0.1 cm) at a concentration $7{\times}10^7$ chondrocytes per $cm^3$. Each Autogenous Cell-polymer constructs were implanted subcutaneously into the left side of dorsum of twelve Rabbits. Polymer templates not containg cells were implanted into the right side as a control. Fifteen rabbits were sacrificed at the following intervals: 5 rabbits at nine weeks, 7 rabbits at twelve weeksNew autogenous cartilage formation which retained the approximate dimensions of origianl round polymer template in 11 of 12 cell seeded implants. Histological examination using hematoxyline and eosin stain revealed vast majority of implants developed into mature cartilage. This study opens up the possibility of autologus cell transplant to construct autogenous cartilge.

Contemporary Characteristics of Fashion-textile Design Applying Paisley Ornament (페이즐리 오너먼트를 활용한 패션텍스타일 디자인의 현대적 특징 연구)

  • Chung, Ha Jeong;Park, Juhee
    • Journal of the Korean Society of Clothing and Textiles
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    • v.44 no.5
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    • pp.950-968
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    • 2020
  • This study derives the modern characteristics of the paisley ornament through an analysis of the paisley ornament shown in fashion since the 2010s based on a theoretical review of the paisley ornament used in Kashmir India shawls for artistic inspiration in the fashion-textile field. It classified the paisley ornament in fashion since the 2010s into types of 'basic structure of body and coif', 'abnormal paisley ornament shapes', 'complex structure of paisley ornament', 'complex structure with other motif', 'a pair of symmetrical reflection motif', 'regular of repetitive arrangement', 'complex arrangement of irregularity and regularity' and 'free arrangement of irregularity'. A comparative analysis with the historical paisley ornament shows that paisley ornaments have contemporary characteristics like 'Bisector structure of centerline in criterion of the body', 'Composite structure of extended 1-repeat', and 'Free structure of engineered placement'. A modern design was inspired by the historical art forms; however, it was used in free utilization of motifs and patterns in terms of size, direction, proportion and space. These were the expression of a design identity that originated from the aesthetic ability and career of a designer as well as the technology of computer programs for the improvements in time and cost efficiency.

A Study on the Structural Performance of Slab-column Joint at Flat Plate Structure Using ECC (고인성 시멘트 복합재를 활용한 플랫플레이트 구조의 슬래브-기둥 접합부 구조성능 연구)

  • Choi, Kwang-Ho;Park, Byung-Chun;Choi, Sung-Woo;Ryu, Deug-Hyun
    • Journal of the Korea Concrete Institute
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    • v.29 no.2
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    • pp.209-216
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    • 2017
  • One of the important considerations in structural designing the flat plate system is ensuring the resistance to punching shear caused by axial loads and the ductile ability to follow horizontal deformation under earthquake. In this study, the ECC (Engineered Cementitious Composite) has been placed in the critical section zone of punching shear at slab-column joint to improve ductility and the advanced details of shear reinforced area nearby critical section zone has been developed using stud and steel fiber. The shear performance tests were performed on the specimens with parameters of fiber type mixed with ECC, stud and steel fiber set into the shear reinforced area in which the failure pattern, joint strength, displacement and strain of the specimen were compared and analyzed. The test results showed that the strength and ductility of specimens with ECC applied to joint were better than those of RC flat plate system. Also, the shear reinforcement effect of stud and the ductility improvement of steel fiber concrete were confirmed in the shear reinforcement area.

The Model of Estimating Spectrum Requirements in Mobile Networks with Data Traffic (데이터 서비스를 위주로 하는 이동통신 망에서의 주파수 소요량 산출 모델)

  • Kim, Dae-Keuk;Yang, Eun-Sam;Kim, Hwa-Jong
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.10 no.2
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    • pp.364-369
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    • 2009
  • Today, as various access technologies appear, for example WiMax, WiBro, the demand for spectrum is increasing rapidly. Spectrums are a national resource with scarcity value so that we need to a]locate them effectively for the national economy and industry. Accordingly, In the paper, we proposed several factors and new model for estimating spectrum requirements of a next generation mobile network with multimedia data services. These are self-similarity characteristics of data traffic, engineered capacity considering QoS, structure of FA increase, asymmetry of data traffic between uplink and downlink, handoff traffic, and uneven traffic pattern among base stations. It can applied mobile WiMax or other wireless broadband systems in general.

A Study on Characteristics of Highway Segments for Recreational Trips Using Principal Analysis (주성분분석을 이용한 고속도로의 여가성 도로구간 판별에 관한 연구)

  • Kim, Young-Il;Chung, Jin-Hyuk;Kum, Ki-Jung
    • Journal of Korean Society of Transportation
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    • v.22 no.2 s.73
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    • pp.87-93
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    • 2004
  • A five-day work week has a great impact on the life styles of employed persons and their families. At the same time, the changes also impact on the transportation system because travel patterns, demand, and pattern of congestion change during weekends. The negative impacts on the transportation system should be examined in order to conceive measures to maintain dependable levels of service during weekends. The first step to pursue the issue is to identify the road segments heavily affected by augmented leisure trips. In this study, characteristics of highway segments are engineered by principal analysis using data from TCS database. Scores from principal analysis are employed to distinguish highway segments for leisure trips from total 197 segments considered in this study. In addition, indexes from principal analysis are proposed to identify highway segments for leisure trips.

Does a Debiasing Manipulation Reduce Over-estimation of Emotional Reaction to Risky Objects? (위험 대상에 대한 충격 편향은 탈 편향 조작에 의해 감소하는가?)

  • Yoon, Ji-Won;Lee, Young-Ai
    • Korean Journal of Cognitive Science
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    • v.22 no.1
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    • pp.39-55
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    • 2011
  • People tend to overestimate their emotional reactions to events such as physical handicap and buying a new car in the future. Students overestimate their reactions to a future grade as compared to their reactions after receiving the grade. Impact bias refers to people's tendency to overestimate the intensity and the duration of emotional reactions to a future event. The present study explored whether impact bias occurs to risky objects such as nuclear energy, genetically engineered food, and mobile phone. Participants were asked to predict their emotional reactions at three time points, that is, at the present, a week after, and a year after. They predicted their reactions before and after two debiasing tasks. The present study demonstrated a different pattern of impact bias at three time points: A largest bias was observed a week after the present. A defocalism manipulation has eliminated the impact bias whereas an adaptation manipulation has not. Several points were discussed regarding the difference between the previous and the present work.

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Genetically Engineered Yeast by Heterologous Transformation and Intergeneric Two-Step Protoplast Fusion for Ethanol Fermentation

  • Kim, Young-Ho;Lee, Jae-Ran;Seu, Jung-Hwn
    • Journal of Microbiology and Biotechnology
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    • v.3 no.4
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    • pp.232-237
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    • 1993
  • A strain of yeast which can convert starch directly to ethanol was developed by the intergeneric protoplast fusion between Schwanniomyces alluvius possessing $\alpha$ amylase as well as glucoamylase with debranching activity and FSC-14-75 which previously had been formed from a heterologous transformation and subsequent intergeneric protoplast fusion. Fusants were selected on minimal medium after protoplasts of auxotrophic mutant of S. alluvius fused with heat-treated protoplasts of FSC-14-75 in the presence of 30%(w/v) PEG and 20 mM $CaCl_2$. The fusion frequency was in the range of $10^{-6}$ order. All fusants tested were intermediate types of parental strains for carbon compound assimilation, and their cell volumes were approximately 1.1 times larger than FSC-14-75 and 1.8 times larger than S. alluvius. The fusants were unable to sporulate like FSC-14-75, while S. alluvius could sporulate. In flask scale the most promising fusant, FSCSa-R10-6, produced 7.83%(v/v) and 10.17%(v/v) ethanol from 15% and 20% of liquefied potato starch, respectively, indicating that the fermetation efficiency of each case increased 1.2 times and 1.6 times than that of FSC-14-75. The elution pattern on DEAE-cellulose chromatography showed that FSCSa-R10-6 has four distinct amylase peaks of which two peaks originated from S. alluvius and the other two from FSC-14-75. These results suggest that the enhanced fermentation efficiency of the fusant might be due to almost-complemented parental amylases.

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A Study of Fashion Design Applying a 3D Print Polymer-Fabric Structure (3DP 폴리머-패브릭(3D Print Polymer-Fabric Structure)을 적용한 패션디자인 연구)

  • Soyung Im;Jaehoon Chun
    • Fashion & Textile Research Journal
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    • v.25 no.2
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    • pp.139-152
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    • 2023
  • Despite efforts to apply 3D print (3DP) technology in the field of fashion and endless discussions about the possibility of future development, in reality, it is difficult to utilize 3DP technology in fashion for reasons related to material, technology, and cost constraints. The purpose of this study was to supplement the limitations of 3DP technology in order to promote its utilization in fashion and simultaneously find a solution to achieve aesthetic satisfaction in the design method. Specifically, through the development of fashion products with a 3DP polymer-fabric structure to which the parametric design methodology has been applied, this study explored the possibility of practical application and proposes a new 3DP fashion design method. The 3DP polymer-fabric developed as a result of the research was stably adhered to the fabric. Additionally, the study confirmed the possibility of making 3DP clothes that are amenable to the wearer's activities, as it was verified that cutting and sewing tailored to the human body's curvature and structure can be performed. The design process using the 3DP polymer-fabric presented in this study is meaningful in that it suggests a solution to complement the limitations of modern technology in connection with designers' creativity. Moreover, the design process presented in this study is expected to contribute to the commercialization and generalization of 3DP by providing practical help to allow fashion experts to utilize 3DP technology.