• 한국작물학회지
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• 제31권1호
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• pp.16-18
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• 1986

보리 F$_2$용 종자에 대한 찰성유전자(wx)와 분장성 전분유전자(fra)에 대한 유전현상을 구명하고 또 이들 두 유전자간에 관계를 구명키 위하여 실험을 한바 그 결과는 아래와 같다. 1 찰성 및 전분상성 배분인자는 각각 1개의 열성유전자가 관여하였으며, 2. 찰성과 분장성 전분유전자는 각각 독립적으로 작용하였다.

• 한국식품영양과학회지
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• 제20권6호
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• pp.637-645
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• 1991

Physicochemical factors affecting cooking and eating quality of rice and their mechanisms were investigated. The stickiness of cooked rice was negatively correlated with amylose content(r=0.58, p<0.05) and protein content(r=-0.72, p<0.01), but not affected by crude fat content of rice. The ultrastructure of cooked rice grain showed the progressive gelatinization of starch from the periphery toward the center of the endosperm as water and heat energy diffused into. The rate of water diffusion appears to be dependent on the cell arrangement in the endosperm and the protein content of milled rice. Once water and heat reach the starch granules, the rate of in situ gelatinization of starches appears to be dependent on their own gelatinization temperature range and amylose content. Protein acts as a barrier for the swelling of starch and water diffusion in two ways : 1) by encasing starch granules in the starchy endosperm, and 2) by forming a barrier between the subaleurone layer and the starchy endosperm. Therefore, the separation and fragmentation of the outermost layers of the endosperm occurred more easily in the low-protein content rices, and was associated with increases of solids lost in cooking-water at 95$^{\circ}C$ and stickiness of cooked rice.

• 한국작물학회지
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• 제39권1호
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• pp.63-72
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• 1994

보리의 이용성을 검토하기 위하여, 찰성 부정형 Shrunken endosperm 유전자를 결합하여 육성한 Isogenic line을 이용하여, 취반 및 제분에 대한 제특성을 조사한 바 그 결과를 요약하면 다음과 같다. 1. 보리의 배유 전분 특성이 다른 Isogenic type 간에 정곡 천립중, 회분, 단백질 함량은 별차이가 없었으나 Amylose함량, $\beta$-glucan viscosity와 취반특성은 뚜렷한 차이를 보였다. 2. 취반특성중 흡수율과 퍼짐성은 천립중, Amylose 함량과 부의 상관이 있었고, 용출고형물량과 점도간에도 부의 상관이 있었다. 3, 흡수율과 퍼짐성은 배유 전분 특성과 입의 크기에 의해 영향을 받았으며 그 결과 찰성 Isogenic type이 높았고 부정형 전분 Isogenic type이 가장 낮았으며 종실의 크기가 작은 것이 큰 것에 비해 높았다. 4. 보리밥의 Mixogram pattern은 전분 특성에 따라 차이가 있었으며 부정형 전분이 가장 많이 으깨어졌다. 5. 제분 특성은 메성이면서 부정형인 Isogenic type이 좋았고 찰성의 Shrunken endosperm Isogenic type은 대단히 불량하였다.

• Journal of Crop Science and Biotechnology
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• 제11권4호
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• pp.227-232
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• 2008

Drought stress is one of the major abiotic stresses in agriculture worldwide. We report here a proteomic approach to investigate the impact of post-fertilization drought on grain quality in rice seed endosperm (Oryza sativa cv. IR-64). Plants were stressed for 4 days at 3 days before heading. Total proteins of endosperm were extracted and separated by two-dimensional gel electrophoresis. Not many protein spots showed differential accumulation in drought-stressed samples. More than 400 protein spots were reproducibly detected, including three that were up-regulated and five down-regulated. Mass spectrometry analysis and database searching helped us to identify six spots representing different proteins. Functionally, the identified proteins were related to protein synthesis and carbohydrate metabolism, such as Granule-Bound Starch Synthase (GBSS, Wx protein), which is thought to play a very important role in starch biosynthesis and quality, a very crucial factor in determining rice grain quality.

• Journal of Plant Biotechnology
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• 제3권3호
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• pp.151-157
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• 2001

In order to breed barley lines with reduced viscosity of wort, a fractured starch mutant of naked barley cultivar, Nubet, was obtained from the M2 seeds mutated by the diethyl sulfate treatment. Seeds of this fractured starch mutant were opaque and the endosperm consists of angular, irregular and fractured starch. The mutant was caused by single recessive mutation and assigned by the symbol fra. The gene was located on chromosome 4, distal in long arm by linkage recombinations using translocation homozygote lethal test set. The linkage value between the fractured starch mutant and 72-4a, 72-4d were 26.0$\pm$4.9, 34.2$\pm$3.1 percent respectively. In addition to the reduced seed size, fewer kernels per spike and higher tillering ability, lower $\beta$-glucan viscosity and higher lysine content of the grain were associated with this mutant. $\beta$-glucan viscosity of the Nubet grains increased from 3 weeks after anthesis to matury and most of the viscose substances appeared to be stored in the middle of the endosperm tissue. Since the mutant grains showed better milling property as compared to Nubet, it can be used as breeding resources to develope new barley cultivars for maltins and milling purpose.

• 한국식품과학회지
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• 제19권1호
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• pp.69-74
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• 1987

본(本) 연구(硏究)는 경(硬), 연질(軟質) 소맥(小麥)의 성숙과정(成熟過程)에 따른 탄수화물(炭水化物) 특성(特性)의 변화(變化)에 대하여 검토하였다. 종실(種實)의 수분함량(水分含量)이 감소(減少)함에 따라 천립중(千粒重)과 용적중(容積重)은 증가(增加)되었다. 배유(胚乳)의 전분함량(澱粉含量)은 출적후(出積後) $35{\sim}40$일까지 급격히 증가(增加)하였으며, 특히 조숙종(早熟種)인 조광(早光)은 B-type 전분(澱粉)의 합성(合成)이 억제되었다. Amylose와 amylopectin 함양(含量)은 성숙기간(成熟期間)에 따라 증가(增加)되었으며, pentosan의 함량(含量)도 계속증가되었다. Amylograph break-down은 성숙기간중(成熟期間中) 계속 감소(減少)되었으며. 배유(胚乳)의 전분함량(澱紛含量)과 고도(高度)의 부동법(負桐關)이 있었다.

• 한국육종학회지
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• 제42권1호
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• pp.1-10
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• 2010

Five mutants were investigated at the molecular level to determine the factors responsible for mutated endosperm types. They were classified as high (HA) or low amylose (LA) phenotypes based on the amylose content in endosperm. The five were previously produced from Ilpum and Shindongjin cultivar treated with N-methyl-N-nitrosourea and gamma-ray irradiation, respectively. Analysis of the genomic structure and expression of Granule-bounded Starch Synthase I (GBSSI) genes revealed that mutants generally showed a higher incidence of nucleotide transition than transversion, and the $A:T{\rightarrow}G:C$ transition was particularly prevalent. The rates of nucleotide substitution in HA mutants were generally higher than those in the LA mutants, leading to higher substitutions of amino acid in the HA mutants. Neither nucleotide substitutions interfering with intron splicing or causing early termination of protein translation were found, nor any large-sized deletions or additions were found in all the mutants. In principle, amylose content can be regulated by three factors: internal alterations of GBSSI protein, the strength of gene expression, and other unknown external factors. Our results indicate that the endosperm mutants from Shindongjin arose from internal alterations of GBSSI proteins, which may be the result of amino acid substitutions. On the other hand, the Ilpum mutants might be principally caused by the alteration of gene expression level. Analysis of another three glutinous cultivars revealed that the major factor leading to glutinous phenotypes is the 23-bp duplicative motif (5'-ACGGGTTCCAGGGCCTCAAGCCC-3') commonly found in exon 2, which results in the premature termination of protein translation leading to the production of a non-functional GBSSI enzyme.

• Applied Biological Chemistry
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• 제39권5호
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• pp.379-383
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• 1996

조생종 쌀인 오대벼 품종을 대상으로 종자의 배유세포에 있는 전분복합체와 단백질체의 외부형태 및 미세 구조의 특징을 광학현미경과 전자현미경으로 관찰하였다. 배유세포는 긴 막대모양으로 세로 단면이 장방형 또는 마름모형을 하고 있고, 세포벽은 $0.5\;{\mu}m$의 두께로 균질한 막상 구조물로 이루어져 있다. 또한, 세포내에는 구형 또는 타원형의 전분복합체가 치밀하게 채워져 있으며, 직경이 $18{\sim}25\;{\mu}m$로 다양한 크기로 존재하였다. 전분복합체는 12면체 또는 14면체의 중앙 전분과립을 중심으로 약 $5\;{\mu}m$ 크기의 전분과립들이 동심원상으로 2 내지 3층으로 형성되어 있다. 배유세포에서 단백질체는 호분층에서 보다 아주 드물게 관찰되었지만, 전분복합체의 주변에 인립하여 산재하고 있다. 단백질체는 직경이 약 $3\;{\mu}m$로 구형 또는 난원형의 형태를 하고 있으며 미세구조적으로 중심부는 전자밀도가 높게, 한계막의 주변부는 비교적 전자밀도가 낮게 관찰되었다.

• 한국작물학회지
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• 제44권2호
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• pp.138-142
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• 1999

An experiment was carried out to find out the changes in morphology, sugars, and $\alpha$-amylase activity during the priming of rice seeds (Oryza sativa L. cv. ＇Ilpumbyeo＇). For priming, seeds were soaked in -0.6 MPa PEG solution at 15$^{\circ}C$ for 4 days (properly primed) and at $25^{\circ}C$ for 4 and 10 days (over-primed) and dried at room temperature. The size of coleoptile and differentiated leaves of properly primed seeds were bigger and coleoptile was separated from the other part of embryo compared with non-primed and over-primed seeds. As priming of seeds advanced, compound starch grains in the endosperm disintegrated into tiny starch granules, and small holes were found in the tiny starch granules and a cavities developed between embryo and endosperm. The radicle and plumule of properly primed germinating seeds developed faster than non-primed and overprimed germinating seeds. Sucrose, maltose, and raffinose contents of properly primed seeds decreased, while content of glucose and fructose and $\alpha$-amylase activity increased. However, sugar content and $\alpha$-amylase activity of over-primed seeds were lower compared with non-primed seeds or properly primed seeds.

• Applied Microscopy
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• 제25권4호
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• pp.71-82
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• 1995

The ultrastructure and storage reserves of the Capsicum annuum seeds were studied in order to identify structure and to localize storage components in the endosperm using light microscopy, scanning and transmission electron microscopy. The seed coat was composed of one cell layer which contained a large number of lipid bodies, while most of the endosperm cells did not showed many lipid bodies. During seed maturation, the endosperm cells were continuously degenerated by the autophagy. Various types of plastids were also distinguished in the endosperm cells. They contained starch grains surrounded by electron-dense tiny particles, plastoglobuli, and vasicular bodies.