• 한국어류학회지
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• 제23권2호
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• pp.150-157
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• 2011

칼납자루를 중심으로 납자루아과 고유종 각시붕어, 한강납줄개, 묵답자루의 재생산을 위한 기초 연구를 하였다. 난의 형태와 부화 자어는 종별로 독특하였으며 암컷의 크기와 1회 산란량의 상관관계는 뚜렷하게 나타나지 않았다. 칼납자루 $13.0^{\circ}C$에서도 산란이 가능하였으나 수정란의 부화율은 43.5%로 낮았으며, 수온 $21.0^{\circ}C$에서 79시간 만에 부화하였고 다른 비교 종들과 같이 수온이 높을수록 부화에 이르는 시간은 단축되었다. 칼납자루는 부화 후 자어 시기를 거쳐 300일 만에 52.4 mm로 성장하여 재생산에 참여할 어미가 되기에 충분하였다. 납자루아과 어류의 재생산 연구는 고유 담수어 보존 연구의 기초자료로 활용될 것이다.

• Fisheries and Aquatic Sciences
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• 제25권12호
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• pp.601-618
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• 2022

The genus Semisulcospira is an economically and ecologically valuable freshwater resource. Among the species, Semisulcospira coreana, Semisulcospira forticosta and Semisulcospira tegulata are endemic to the Korean peninsula and Semisulcospira gottschei is widespread in Asia. Therefore, maintenance and conservation of wild populations of these snails are important. We investigated the genetic diversity and population structure of Semisulcospira based on the mitochondrial cytochrome c oxidase subunit I (COI), NADH dehydrogenase subunit 4 (ND4), and combined mitochondrial DNA (COI + ND4) sequences. All four species and various genetic makers showed a high level of haplotype diversity and a low level of nucleotide diversity. In addition, Fu's Fs and Tajima's D neutrality tests were performed to assess the variation in size among populations. Neutrality tests of the four species yielded negative Fu's Fs and Tajima's D values, except for populations with one haplotype. The minimum spanning network indicated a common haplotype for populations of S. coreana, S. tegulata and S. gottschei, whereas S. forticosta had a rare haplotype. Also, genetic differences and gene flows between populations were assessed by analysis of molecular variance and using the pairwise fixation index. Our findings provided insight into the degree of preservation of the species' genetic diversity and could be utilized to enhance the management of endemic species.

• 식물분류학회지
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• 제51권1호
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• pp.109-114
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• 2021

Veronica nakaiana Ohwi (Plantaginaceae) is an endemic taxon on Ulleungdo Island, Korea. We report the second complete chloroplast genome sequence of V. nakaiana. Its genome size is 152,319 bp in length, comprising a large single-copy of 83,195 bp, a small single-copy of 17,702 bp, and a pair of inverted repeat regions of 25,711 bp. The complete genome contains 115 genes, including 51 protein-coding genes, four rRNA genes, and 31 tRNA genes. When comparing the two chloroplast genomes of V. nakaiana, 11 variable sites are recognized: seven SNPs and four indels. Two substitutions in the coding regions are recognized: rpoC2 (synonymous substitution) and rpl22 (nonsynonymous substitution). In nine noncoding regions, one is in the tRNA gene (trnK-UUU), one is in the intron of atpF, and seven are in the intergenic spacers (trnH-GUG~psbA, trnK-UUU, rps16~trnQ-UUG, trnC-GCA~petN, psbZ~trnG-GCC, ycf3~trnS-GGA, ycf4~cemA, and psbB~psbT). The data provide the level of genetic variation in V. nakaiana. This result will be a useful resource to formulate conservation strategies for V. nakaiana, which is a rare endemic species in Korea.

• 한국균학회지
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• 제51권4호
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• pp.405-410
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• 2023

Erysiphe chloranthi is the only powdery mildew species (Erysiphaceae) found on Chloranthus plants belonging to the family Chloranthaceae. This species has long been known to be present in Japan and Korea and is hence regarded as endemic to East Asia. Examination of seven Korean specimens revealed detailed morphological characteristics of the holomorph of this species. Short foot-cells of conidiophores are proposed as key characteristics that differ from related species. Sequences of the internal transcribed spacer regions and large subunit gene retrieved from four Korean samples confirm the phylogenetic position of this species within the Erysiphe aquilegiae complex. These data may help elucidate the identity of this species and clarify the ambiguity of the E. aquilegiae complex.

• Journal of Ecology and Environment
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• 제29권3호
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• pp.247-252
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• 2006

울릉도의 특산 식물종으로서 희귀식물종이며, 춘계 단명성 식물로서 개미에 의해 종자 산포가 이루어지는 섬현호색(Corydalis filistipes Nakai) 4개의 아집단에 대하여 합리적인 보전 및 관리 대책 수립을 위하여 9 개의 동위효소 marker를 이용하여 유전적 다양성과 구조를 분석하였다. 그 결과 평균 대립 유전자의 수(A)는 1.73개, 95% 수준에서 다형적 유전자좌의 비율은(P)은 61.2%, 이형접합체의 평균 관측치(Ho)는 0.201, 기대치(He)는 0.167로서 분포역이 넓은 특산 식물 종들에 비해서는 낮은 수준이지만, 섬에 고립된 유사한 생활사를 갖는 특산식물 종들에 비해서는 높은 유전적 다양도를 유지하고 있는데, 그 이유는 소집단이기는 하나 적정수준의 개체수가 유지되고 있고, 타가수정을 주로 하며 개미에 의해 종자 산포가 이루어져 적응력이 높고, 또한 폐쇄화에 의한 유 무성 번식 체계를 겸하기 때문인 것으로 판단된다. 그리고 유전적 구조분석 결과 아집단내($F_{IS}$)와 전체 아집단($F_{IT}$)의 근친교 배계수가 각각 -0.1889 와 -0.1226로서 H-W의 평형구조에 비해 이형접합자의 빈도가 높았으며, 아집단간 유전적 분화도는 매우 낮은($F_{ST}=0.0557$)결과를 보였다. 그리고 우리나라 희귀 및 특산 식물종인 섬현호색의 유전적 변이의 유지 기작과 합리적인 보전과 관리 대책을 논의하였다.

• 한국자원식물학회지
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• 제22권6호
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• pp.558-570
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• 2009

The vascular plants in Jeju composed of 21 families 62 genera, 190 species, 7 varieties with 197 taxa in Pteridophyta, 3 families 5 genera, 7 species, 3 forma with 10 taxa in gymnosperm (Coniferophyta) and 143 families 703 genera, 1,622 species, 114 varieties and 47 forma with 1,783 taxa in Endospermae. The total number of taxa was 1,990 consisting 167 families, 770 genera, 1,818 species, 121 varieties and 50 forma. Three families, 4 genera 4 species and 1 variety in Pteridophyta and 1 family, 1 genus I species, 1 forma of Coniferophyta were endemic to Jeju. Five families, 8 genera and 8 species in the Monocotyledonae of Endospermae and 23 families, 32 genera, 21 species, 16 varieties and 7 forma and 44 taxa in Cholipetalae, and 9 families, 24 genera, 12 species, 13 varieties and 7 forma, total 32 taxa in Sympetalae were endemic to Jeju. Total 90 taxa with 41 families, 69 genera, 46 species, 29 varieties and 15 forma were drawn up as endemic plants in Jeju. As the numbers show on, Jeju has more biodiversity especially plant diversity than any other places in Korea. It was an important region in regard to geographical position. The rarity on plants from Jeju has been assessed based on the IUCN red list categories and criteria at both regional and global levels. There was one species in each extinct (EX) and extinct in the Wild (EW). The EX and EW species were Rhododendron saisiuense Nakai (Ericaceae) and Asplenium antiquum Makino (Aspleniaceae), respectively. Three taxa, Rhododendron dauricum L. (Ericaceae), Lycopodium sieboldii Miq., and Lycopodiella cernua (L.) Serm (Lycopodiaceae) were species that is extinct in the region (RE). Sixty-one taxa were critically endangered (CR), 13 were endangered (EN), and 83 were vulnerable (VU) at regional level. The taxa listed on a Red List corresponds 26.9% of total taxa in Jeju. At global level, there were 19 taxa in CR, 4 in EN, and 1 in VU (Vulnerable) as the category of threat. The rest taxa (133 taxa) were classified as Least Concern (LC). According to the results of the assessment, conservation measures must be taken for total of 157 species that were categorized in threaten including one of EX, one of EW and three extinct in the region immediately. Of 157 species, 61 were CR, 13 are EN and 83 were VU.

• 한국응용곤충학회지
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• 제38권1호
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• pp.1-10
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• 1999

동물지리학적 분석결과 한국산 종들의 분포 양상을 보면 Holarctic(9.6%), Transpalearctic(38.4%), Transpalearctic-Oriental(8.2%), Eastern Palearctic(Trans-Far East Asia)-Oriental(4.1%), Eastern Palearctic(17.8%)과 Endemic(21.9%)으로 나타났다. 한국산 뭉툭맵시벌아과는 구북계에서 종들과 고유종의 분포가 많은 것으로 보아 북반구에서 도래한 것으로 여겨진다. 뭉툭맵시벌아과의 가장 오래된 화석종은 Catachora minor Townes, Urotrophon Pusillus Townes 등 2종이다. 뭉툭맵시벌아과의 전세계의 분포는 대륙이동과 거의 유사하게 이루어 졌다고 여겨진다.

• 환경생물
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• 제17권1호
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• pp.79-88
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• 1999

1994년부터 1996년도까지 계류성 독립하천인 웅천천 수계의 보령댐 수몰예정지역을 중심으로 하여 6개지점에서 어류상, 주목할만한 어종 및 어류군집에 대하여 조사하였다. 본 조사지역에서 94년에서 96년 사이에 채집을 통하여 서식이 확인된 종은 11과 25속으로 총 29종이었으며 이중 9종은 한국특산어종이었다. 이 중 멸종위기종인 감돌고기와 퉁사리, 특산어종인 꺽지는 본 지역에서 확인되지 않아 절멸된 것으로 생각된다. 우세어종은 갈겨니, 피라미, 긴몰개가 우세한 그룹의 어종으로 나타났다. 지점별 평균풍부도는 0.68개체/$m^2$, 평균체적밀도는 1.45개체/㎥로 나타났으며, 종의 다양도는 상류와 하류보다는 중류가 가장 높게 나타났다. 군집간 유사도는 1군집과 4군집, 5군집과 6군집이 유사하였으나 유의한 차이는 나타내지 않아 전체가 유사한 군집으로 나타났다.

• Journal of Species Research
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• 제11권1호
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• pp.29-37
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• 2022

This study is based on crane fly specimens collected more than 80 years ago in 1938-1939 and preserved at the United States National Museum, Smithsonian Institution, Washington DC, USA. Despite many attempts with a variety of methods, no additional specimens of this genus were captured. This likely means that that this genus is extinct on the Korean Peninsula, or its distribution is restricted to the northern areas of the Democratic People's Republic of Korea (North Korea). The genus Prionolabis Osten Sacken, 1860 with four species, one of them Prionolabis dis(Alexander, 1950) endemic to North Korea, was recorded by Ch. P. Alexander (Alexander, 1938, 1940, 1950). All succeeding papers listing these species were based on these original works without study based on actual specimens. For each species, we present general information on genus, redescriptions of species based on Korean specimens, illustrations of important taxonomical structures, elevation range, period of activity, habitat information, general distribution, and a distribution map for the Korean Peninsula.

• 대한미생물학회지
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• 제34권5호
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• pp.491-500
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• 1999

A recent study showed that comparative sequence analysis of rpoB DNAs could reveal natural relationships in genus Mycobacterium [J Clin Microbial. 37 (6). 1999]. rpoB DNAs showed interspecies variation and intraspecies conservation. Based on these data, we developed polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) protocols which enable species differentiation in genus Mycobacterium. When this assay was applied to 24 clinical isolates identified as M. avium complex (MAC) by biochemical test, these were successfully differentiated into M. avium and M. intracellulare. These results were concordant with those obtained by 16s rDNA analysis. It is the first report that PCR-SSCP analysis of rpoB DNA could be used for species differentiation of MAC strains.