The Journal of the Korean Society for Microbiology (대한미생물학회지)

The Korea Society for Microbiology (대한미생물학회)
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Identification of Mycobacterium avium complex (MAC) Clinical Strains to a Species Level by Sequencing and PCR-SSCP Analysis of rpoB DNA

비결핵항산성균의 rpoB DNA 염기서열과 SSCP pattern 분석에 따른 Mycobacterium avium complex (MAC) 임상분리균주의 동정

  • Kim, Bum-Joon (Department of Microbiology, Cheju National University College of Medicine) ;
  • Lee, Seung-Hyun (Department of Microbiology and Institute of Endemic Diseases (SNUMRC), Seoul National University College of Medicine) ;
  • Lee, Kuen-Hwa (Department of Microbiology and Institute of Endemic Diseases (SNUMRC), Seoul National University College of Medicine) ;
  • Park, Chung-Kyu (Department of Microbiology and Institute of Endemic Diseases (SNUMRC), Seoul National University College of Medicine) ;
  • Choi, Myung-Sik (Department of Microbiology and Institute of Endemic Diseases (SNUMRC), Seoul National University College of Medicine) ;
  • Kim, Ik-Sang (Department of Microbiology and Institute of Endemic Diseases (SNUMRC), Seoul National University College of Medicine) ;
  • Choi, Sung-Bai (Department of Microbiology and Institute of Endemic Diseases (SNUMRC), Seoul National University College of Medicine) ;
  • Hwang, Eung-Su (Department of Microbiology and Institute of Endemic Diseases (SNUMRC), Seoul National University College of Medicine) ;
  • Cha, Chang-Yong (Department of Microbiology and Institute of Endemic Diseases (SNUMRC), Seoul National University College of Medicine) ;
  • Kim, Sang-Jae (The Korean Institute of Tuberculosis, the Korean National Tuberculosis Association) ;
  • Bai, Gill-Han (The Korean Institute of Tuberculosis, the Korean National Tuberculosis Association) ;
  • Kook, Yoon-Hoh (Department of Microbiology and Institute of Endemic Diseases (SNUMRC), Seoul National University College of Medicine)
  • 김범준 (제주대학교 의과대학 미생물학 교실) ;
  • 이승현 (서울대학교 의과대학 미생물학교실 및 의학연구원 감염성 연구소) ;
  • 이근화 (서울대학교 의과대학 미생물학교실 및 의학연구원 감염성 연구소) ;
  • 박정규 (서울대학교 의과대학 미생물학교실 및 의학연구원 감염성 연구소) ;
  • 최명식 (서울대학교 의과대학 미생물학교실 및 의학연구원 감염성 연구소) ;
  • 김익상 (서울대학교 의과대학 미생물학교실 및 의학연구원 감염성 연구소) ;
  • 최성배 (서울대학교 의과대학 미생물학교실 및 의학연구원 감염성 연구소) ;
  • 황응수 (서울대학교 의과대학 미생물학교실 및 의학연구원 감염성 연구소) ;
  • 차창룡 (서울대학교 의과대학 미생물학교실 및 의학연구원 감염성 연구소) ;
  • 김상재 (결핵연구원) ;
  • 배길한 (결핵연구원) ;
  • 국윤호 (서울대학교 의과대학 미생물학교실 및 의학연구원 감염성 연구소)
  • Published : 1999.10.30
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Abstract

A recent study showed that comparative sequence analysis of rpoB DNAs could reveal natural relationships in genus Mycobacterium [J Clin Microbial. 37 (6). 1999]. rpoB DNAs showed interspecies variation and intraspecies conservation. Based on these data, we developed polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) protocols which enable species differentiation in genus Mycobacterium. When this assay was applied to 24 clinical isolates identified as M. avium complex (MAC) by biochemical test, these were successfully differentiated into M. avium and M. intracellulare. These results were concordant with those obtained by 16s rDNA analysis. It is the first report that PCR-SSCP analysis of rpoB DNA could be used for species differentiation of MAC strains.

Keywords

References

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