• Journal of Species Research
• /
• 제9권1호
• /
• pp.1-10
• /
• 2020

A new dragonfly species, Nannophya koreana sp. nov., is described from Korea on the basis of morphology and mitochondrial cytochrome oxidase c subunit I (COI) gene sequences. Nannophya materials from Korea and other areas in Southeast Asia were compared. The new species was previously recognized in Korea as the endangered pygmy dragonfly Nannophya pygmaea Rambur, 1842, which is widely distributed in insular and peninsular Southeast Asia. However, male adults of the Nannophya population in Korea could be distinguished from other N. pygmaea populations by the presence of a thick, incomplete black stripe on the lateral synthorax that terminated at half-length (vs. continuous to wing base), light orange (vs. red) anal appendages, and 4-5 (vs. 2-3) black teeth on the ventral superior appendages. In addition, the body length of N. koreana was generally larger (1.2-1.4 times) than that of N. pygmaea, regardless of life stage. COI gene sequences from the two groups exhibited substantial genetic differences (>12%), thereby sufficiently substantiating their differentiation. The taxonomic status, distribution, and habitat of the new species are discussed.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제15권1호
• /
• pp.47-58
• /
• 2007

The tiny dragonfly, Nannophya pygmaea(Odonata: Libellulidae) is one the smallest dragonflies in the world and listed as a second-degree endangered wild animal and plant in Korea. For the long-term conservation of such endangered species, an investigation on nation-wide genetic magnitude and nature of genetic diversity is required as a part of conservation strategy. We, thus, sequenced a portion of mitochondrial COI gene, corresponding to "DNA Barcode" region(658 bp) from 68 N. pygmaea individuals collected over six habitats in Korea. The sequence data were used to investigate genetic diversity within populations and species, geographic variation within species, phylogeographic relationship among populations, and phylogenetic relationship among haplotypes. Phylogenetic analysis and uncorrected pairwise distance estimate showed overall low genetic diversity within species. Regionally, populations in southern localities such as Gangjin and Gokseong in Jeollanamdo Province showed somewhat higher genetic diversity estimates than those of remaining regions in Korean peninsula. Although geographic populations of N. pygmaea were subdivided into two groups, distance- or region-based geographic partition was not observed.

• International Journal of Industrial Entomology and Biomaterials
• /
• 제35권1호
• /
• pp.51-57
• /
• 2017

The tiny dragonfly, Nannophya pygmaea (Odonata: Libellulidae), is an endangered insect in South Korea. Previously, a partial mitochondrial DNA sequence that corresponded to a DNA barcoding region has been used to infer genetic diversity and gene flow. In this study, we additionally sequenced the barcoding region from N. pygmaea that had been collected from three previously sampled populations (40 individuals) and these sequences were combined with the preexisting data. We also selected and sequenced an additional mitochondrial gene (ND5) to find further variable gene regions in the mitochondrial genome. DNA barcoding sequences of 108 individuals from five South Korean localities showed that genetic diversity was highest in Gangjin, Jeollanam-do Province. Muuido, which was previously occupied by a single haplotype, was also found to have an identical haplotype, which confirmed the low genetic diversity on this islet. Gene flow among populations is highly limited, and no clear distance- or region-based geographic partitioning was observed. Phylogenetic relationships among haplotypes showed that there were no discernable haplotypes in South Korea. ND5 provided slightly more haplotypes compared to the barcoding region in 40 individuals (14 vs. 10 haplotypes in the COI gene). It also had a slightly higher within-locality diversity estimate, which suggested that ND5 had potential as mitochondrial DNA-based marker for population genetic analysis.

• 환경생물
• /
• 제35권4호
• /
• pp.545-548
• /
• 2017

Nannophya pygmaea (commonly known as the scarlet dwarf dragonfly) was designated as an endangered species, level II, by the Ministry of Environment of Korea in 1994; it has been used as a flagship species for the protection and preservation of wetlands. Over 25 sites in Korea have been identified as the habitat of Nannophya pygmaea. However, most of these habitats have proven to be unstable, and there have been subsequent changes in the assemblage structure and organization. Most habitats changed to become grasslands or plain ground, and now only five habitats remain. Although efforts have been made to protect the Nannophya pygmaea as an endangered species, their habitat loss has increased, caused by natural succession and drought. Therefore, we need to make stronger protections in the preservation manual of level II endangered species, particularly Nannophya pygmaea, and its native habitats in Korea.

• Journal of Ecology and Environment
• /
• 제46권4호
• /
• pp.324-333
• /
• 2022

Background: The Korean scarlet dwarf, Nannophya koreana Bae (Odonata: Libellulidae), is anendangered dragonfly with an increasing risk of extinction owing to rapid climate changes and human activities. To prevent extinction, the N. koreana population and their habitat should be protected. Therefore, suitable habitat evaluation is important to build the N. koreana restoration project. The habitat suitability index model (HSI) has been widely used for habitat evaluation in diverse organisms. Results: To build a suitable HSI model for N. koreana, 16 factors were examined by seven experienced researchers. A field survey for N. koreana observed sites and spatial analysis were conducted to improve the model. Five factors were finally selected by this procedure (crown density, open water surface, water depth, pioneer plant cover, and type of water source). Finally, the N. koreana HSI model was generated with the five adjusted factors based on interview, field survey, and spatial analysis. This model was validated by a current N. koreana habitat in 2021. With this model, 46 sites in Uljin-gun, Korea, were surveyed for N. koreana habitats; five sites were identified as core habitats and seven as potential core habitats. Conclusions: This model will serve as a strong foundation for the N. koreana restoration project and as a reference for future studies on N. koreana and other endangered insect populations. Further analysis and long-term data will improve the efficacy of this model and restore endangered wildlife.

• 환경생물
• /
• 제27권3호
• /
• pp.292-296
• /
• 2009

본 연구는 한국의 멸종위기곤충인 꼬마잠자리 알의 발육과 온도의 관계를 추정하고자 8가지 서로 다른 온도 조건(17, 20, 22, 25, 28, 30, 33 및 $36^{\circ}C$)에서 실시하였다. 꼬마잠자리의 알은 2007년 6월 경상북도 문경시 일대의 산간 습지에 서식하는 암컷 성충으로부터 채취하였다. 실험의 결과, 부화율은 17, 20, 22, 25, 28, 30, 33, 그리고 $36^{\circ}C$에서 각각 2.86, 17.09, 24.32, 39.67, 34.43, 40.57, 44.79 및 1.75%였다. 각 온도에서의 알의 발달율(평균발육속도)은 변형된 Sharpe와 DeMichele의 비선형 모형에 부합하였다. 알의 저온발육임계온도는 $14.02^{\circ}C$(y=0.005988x-0.084, $r^2$=0.99)로 추정되었고, 발육최적온도는 $30{\sim}35^{\circ}C$로 추정되었다.

• 생태와환경
• /
• 제42권3호
• /
• pp.290-294
• /
• 2009

본 연구에서는 꼬마잠자리 유충의 성장률을 밝히고자 인공습지서식처를 조성하고 자연개체군을 이식하여 실험하였다. 인공서식처는 꼬마잠자리가 서식하고 있는 충남 보령시의 자연서식처를 모방하여 온실(비닐하우스)안에 조성하였다. 2007년 6월에 300개체의 꼬마잠자리 유충을 인공서식처에 도입하였으며, 2007년 6월부터 2008년 11월까지 유충의 체장을 측정하고자 5차례의 재포획을 실시하였다. 실험의 결과, 처음 개체군과 재포획 개체군(재포획 I, II, III, IV, V 개체군)에서 모두 체장의 크기가 2개의 그룹으로 나뉘어졌다[처음 개체군 6.20$\pm$0.34 mm 및 7.94$\pm$0.46 mm (평균$\pm$분산); 재포획 I 12.84$\pm$0.43 mm 및 5.16$\pm$0.83 mm; 재포획 II 5.96$\pm$0.66 mm 및 8.02$\pm$0.35 mm; 재포획 III 5.97$\pm$0.73 mm 및 7.82$\pm$0.37 mm; 재포획 IV 7.04$\pm$0.93 mm 및 8.52$\pm$0.39 mm; 재포획 V 5.72$\pm$0.60 mm 및 7.71$\pm$0.30 mm]. 본 실험의 결과는 재포획 I$\sim$V의 개체군은 처음 개체군의 자손이며, 이들은 온일도가 470 일 때 약 3mm성장한 것으로 나타났다. 따라서 꼬마잠자리의 성장률은 온일도가 100일 때 약 0.7 mm 자라는 것으로 추정된다.

• Journal of Ecology and Environment
• /
• 제42권4호
• /
• pp.150-154
• /
• 2018

In lotic wetlands, runner reed (Phragmites japonicus) plays a role as a pioneer, which helps other plant species to settle by making dense roots trapping floating-sediments. In lentic wetlands, on the other hand, P. japonicus could play a role as an invader threatening biodiversity by forming tall and dense stands. To conserve an abandoned paddy terrace in mountainous areas, a habitat of an endangered dragonfly species (Nannophya pygmaea), from the monotypic-occupation by P. japonicus, we applied three kinds of treatment: (1) hand-clipping in 2009, (2) mechanical excavating in 2012, and (3) planting of alternative vegetational unit in 2012. We have monitored vegetation changes in the wetland in 2008~2012 and 2017. Vegetation cover of P. japonicus sharply decreased from 43% in 2011 to 16% in 2012 by the mechanical excavation. After 5 years from applying the treatment, Schoenoplectiella mucronata that was utilized in the planting became the predominant species instead of P. japonicus and the number of wetland plant species increased from 16 to 25 with the shift in species composition. This study showed the utility of three control methods of P. japonicas in a lentic wetland.

• 한국응용곤충학회지
• /
• 제45권3호
• /
• pp.381-383
• /
• 2006

멸종위기종인 꼬마잠자리의 부화율을 5개의 온도조건($10,\;15,\;20,\;25,\;30^{\circ}C$)에서 조사하였다. 꼬마잠자리의 알은 2006년 7월 경상북도 문경시 일대의 한 작은 습지에 서식하는 암컷 성충으로부터 채취하였다. 부화율은 100일 동안 부화한 유충의 수로부터 구하였다. 꼬마잠자리의 알은 20, 25 및 $30^{\circ}C$의 온도조건에서 각각 83, 89 및 76%가 부화하였으며, $10^{\circ}C$와 $15^{\circ}C$에서는 실험기간동안 부화하지 않았다. 꼬마잠자리 알의 부화 임계온도는 $14.3^{\circ}C$로 추정되었으며, 다른 온대성 잠자리류보다 상대적으로 높았다.

• 한국환경생태학회지
• /
• 제22권1호
• /
• pp.35-42
• /
• 2008

본 연구는 꼬마잠자리 서식처의 자연환경을 조사하고, 생물종의 복원과 유지를 위한 기초자료로 활용하고자 수행하였다. 꼬마잠자리는 잠자리과에 속하는 초소형 잠자리로서 우리나라에서는 멸종위기 보호야생동식물로 분류되어 보호되고 있다. 국내의 서식처는 중남부 일부 지역에 매우 희소하게 분포되어 있는 것으로 알려졌다. 이러한 멸종위기종인 꼬마잠자리의 서식처 복원을 위한 자료수집을 위해 꼬마잠자리의 서식이 확인된 4개 지역 10개 지점의 경관, 서식처 면적, 식생 유형, 수환경, 수원 등을 조사하였다. 조사는 2006년 6월부터 2007년 8월에 걸쳐 이루어졌다. 꼬마잠자리의 서식처는 대부분 산지의 묵논에 위치하고 있었으며, 서식처 면적은 $113.4m^2\sim1,153.1m^2$로 나타났다. 식생은 골풀과 고마리가 우점종으로 나타났으며, 수심은 $2.6\sim7.3cm$ 사이였고, 수온은 $16\sim27.8^{\circ}C$이었다. 꼬마잠자리의 서식처는 고산 습지에 위치하는 것으로 보고된 바 있으나, 조사 결과 해발 고도 $139\sim243m$ 사이의 지역에 서식하고 있는 것으로 확인되었다. 결론적으로 꼬마잠자리의 서식처는 주변이 위요된 육화되기 전의 빈영양화 초기 단계의 묵논과 같은 습지에 복원되어야 하며, 꼬마잠자리는 다른 잠자리보다 환경에 예민한 습성을 가지고 있기 때문에 이러한 사항들을 고려하여 서식처 복원에 접근하는 것이 필요하다.