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Mitochondrial DNA Sequence Variation of the Tiny Dragonfly, Nannophya pygmaea(Odonata: Libellulidae)  

Kim, Ki-Gyoung (Biological Resources Research Department, National Institute of Biological Resources)
Jang, Sang-Kyun (College of Agriculture & Life Sciences, Chonnam National University)
Park, Dong-Woo (College of Agriculture & Life Sciences, Chonnam National University)
Hong, Mee-Yeon (College of Agriculture & Life Sciences, Chonnam National University)
Oh, Kyoung-Hee (Biological Resources Research Department, National Institute of Biological Resources)
Kim, Kee-Young (Department of Agricultural Biology, National Institute of Agricultural Science & Technology, Rural Development Administration)
Hwang, Jae-Sam (Department of Agricultural Biology, National Institute of Agricultural Science & Technology, Rural Development Administration)
Han, Yeon-Soo (College of Agriculture & Life Sciences, Chonnam National University)
Kim, Ik-Soo (College of Agriculture & Life Sciences, Chonnam National University)
Publication Information
International Journal of Industrial Entomology and Biomaterials / v.15, no.1, 2007 , pp. 47-58 More about this Journal
Abstract
The tiny dragonfly, Nannophya pygmaea(Odonata: Libellulidae) is one the smallest dragonflies in the world and listed as a second-degree endangered wild animal and plant in Korea. For the long-term conservation of such endangered species, an investigation on nation-wide genetic magnitude and nature of genetic diversity is required as a part of conservation strategy. We, thus, sequenced a portion of mitochondrial COI gene, corresponding to "DNA Barcode" region(658 bp) from 68 N. pygmaea individuals collected over six habitats in Korea. The sequence data were used to investigate genetic diversity within populations and species, geographic variation within species, phylogeographic relationship among populations, and phylogenetic relationship among haplotypes. Phylogenetic analysis and uncorrected pairwise distance estimate showed overall low genetic diversity within species. Regionally, populations in southern localities such as Gangjin and Gokseong in Jeollanamdo Province showed somewhat higher genetic diversity estimates than those of remaining regions in Korean peninsula. Although geographic populations of N. pygmaea were subdivided into two groups, distance- or region-based geographic partition was not observed.
Keywords
Mitochondrial DNA; COI gene; Nannophya pygmaea; Tiny dragonfly; Endangered species; Population genetic structure; Conservation genetics;
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