• 한국동물위생학회지
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• 제34권2호
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• pp.159-166
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• 2011

This study was carried out to develope enzyme-linked immunosorbent assay (ELISA) for detection of canine brucellosis in dogs experimentally inoculated with Brucella abortus 1119-3 and B. canis RM666. Groups A, B and C of dogs (each group consisting of three dogs) were orally inoculated with approximately $5{\times}10^9$ colony-forming units of B. abortus and B. canis, and with sterile pyrogen-free PBS, respectively. The animals were monitored at regular intervals upto the 12th week post inoculation (PI) by standard tube agglutination test (STAT), plate agglutination test (PAT), Rose Bengal test (RBT), 2-mercaptoethanol rapid slide agglutination test (2ME-RSAT) and ELISA. The induced antibody titers in group A dogs were detected from the first week PI to the eighth week PI in STAT, PAT and RBT using the inactivated whole cells of B. abortus 1119-3 as antigens, while no sera in groups B and C dogs reacted with the antigens. In 2ME-RSAT using whole cells of B. canis M-strain as antigens, the induced antibody titers in group B dogs were observed at the second week PI and persisted for the 12th week PI, while sera of groups A and C dogs did not react with the whole cells. In ELISA using cytoplasmic fractions antigen of B. abortus 1119-3, the mean optical density of antibodies in groups A and B was detected from the first and second weeks PI, respectively, and persisted for 12th week PI, while sera of group C did not cross-react with the fractions antigen. However, in ELISA using the hot saline extracts of B. canis M- as an antigen, the induced antibody titers in only group B dogs were detected from second week PI and persisted for until the end of this study. These results indicate that the ELISA using B. abortus 1119-3 cytoplasmic fractions as antigens can be a good candidate for detection of brucellosis by B. abortus as well as B. canis in dogs.

• 한국동물위생학회지
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• 제33권1호
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• pp.29-35
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• 2010

This study was performed to test the hypothesis that Brucella abortus strain RB51 (SRB51) might protect Korean indigenous mongrel dog against challenge with either virulent B. abortus biotype 1 or B. canis. A total of 12 Korean mongrel dogs were divided into four groups (Group A, B, C and D). Dogs belonging to Group A and C were inoculated subcutaneously with $1{\times}10^9$ CFU of SRB51 in 1ml of sterile phosphate buffered saline (PBS). Dogs of Group B and D were inoculated subcutaneously with 1ml of sterile PBS as control. At 12 weeks post vaccination, dogs of Group A and B were challenged by oral inoculation of virulent strain of B. canis ($5.0{\times}10^9$ CFU) and dogs of Group C and D were challenged by oral inoculation of virulent strain of B. abortus biotype 1 ($4.4{\times}10^{10}$ CFU). The serum antibodies titers in all dogs were monitored at regular interval for eight weeks after challenge (AC) by standard tube agglutination test, plate agglutination test, rose bengal test, 2-mercaptoethanol rapid slide agglutination test and 2-mercaptoethanol tube agglutination test. No antibody titers in Group A and C was detected. Also, the challenge strains were not found from blood of all dogs of Group A and C from 1 week AC till the end of the experiment by culture and modified AMOS-PCR, whereas B. canis and B. abortus challenge strains were detected from blood of Group B and D, respectively. In addition, neither of two challenge bacteria was recovered from liver, spleen, kidneys, lymph nodes and reproductive tracts of Group A and C dogs after postmortem. However, B. canis and B. abortus challenge strains were isolated from these tissues of Group B and D, respectively. These data suggest that SRB51 could be a promising vaccine candidate for immunizing dogs to control canine brucellosis caused by B. canis or B. abortus.

• 설비공학논문집
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• 제8권1호
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• pp.1-12
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• 1996

In lots of application to heat exchanger systems, closed two-phase thermosyphons are tilted from a horizontal. If the tilt angle, especially, is less than 30$^{\circ}$, the operational performances of thermosyphon are highly dependent on tilt angle. The present study was conducted to better understand such operational behaviors as mech-anni는 of phase change, and flow patterns inside a tilted thermosyphon. For experiment, an ethanol thermosyphon with a 35% of fill charge rate was designed and manufactured, using a copper tube with a diameter 19mm and a length 1500mm. Through a series of test, the tilt angle was kept constant at each of 4 different values in the range 10~25deg. and the heat supply to the evaporator was stepwisely increased up to 30㎾/$m^2$. When a steady state was established to the thermosyphon for each step of thermal loads, the wall temperature distribution and vapor temperature at the condenser were measured. The wall temperature distributions demonstrated a formation of dry patch in the top end zone of the evaporator, with a values of temperature 20~4$0^{\circ}C$ higher than the wetted surface for a moderate heat flux q≒20㎾/$m^2$. Inspite of the presence of hot dry patch, however, the mean values of boiling heat transfer coefficient at the evaporator wall were still in a good agreement with those predicted by Rohsenow's formula, which was based on nucleate boiling. For the condenser, the wall temperatures were practically uniform, and the measured values of condensation heat transfer coefficient were 1.7 times higher than the predicted values obtained from Nusselt's film condensation theory on tilted plate. Using those two expressions, a correlation was formulated as a function of heat flux and tilt angle, to determine the total thermal resistance of a tilted thermosyphon. The correlation formula showed a good agreement with the experimental data within 20%.

• 한국항해항만학회지
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• 제27권1호
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• pp.63-70
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• 2003

초대형 부유식 구조무？ 상부구조는 육상 구조물과는 달리 파량하중의 영향을 받기 때문에 하부부체의 변형에 의해서 상부구조물에는 부가 모멘트가 크게 발생한다. 이와 같은 부가모멘트의 저감을 위하여 보-기둥 접합부에 반강접의 도입에 관한 연구는 시작단계이며 반강접의 비선형 거동을 고려한 상부구조물의 연구는 초기단계이다. 본 논문에서는 초대형 부유식 구조물의 상부구조물에 정적하중과 진폭의 크기가 다른 파랑하중이 동시에 작용할 경우 강접 골조와 부분적으로 반강접 접합부가 사용된 세 가지 접합부 종류에 대한 2차 탄성해석을 수행하였다. 접합부는 웨브에 더블 앵글을 가진 상하 앵글(TSD) 접합과 확장 엔드 플레이트 접합 그리고 각형강관 외다이아프램 접합부를 적용하였으며 중고층 구조물에 파랑하중이 작용할 경우 반강접 접합부의 위치에 따른 모멘트와 수평변위의 응답특성에 대하여 연구하였다.

• 한국식품저장유통학회지
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• 제18권4호
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• pp.429-435
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• 2011

The demand of packaged baby leaves has been increased for its convenient use as fresh-cut produce. This investigation was aimed to explore the effects of different sanitizers on the quality parameters of 'Tah Tasai' Chinese cabbage (Brassica campestris var. narinosa) baby leaves. Thirteen days old baby leaves were harvested and washed in tap water (TW), 100 ppm chlorine solution (Cl), 2 ppm ozonated water ($O_3$), 15 ppm chlorine dioxide solution ($ClO_2$) and washing with 0.2% citric acid solution followed by 50% ethanol spray (CA+Et). The samples were then packaged in 50 ${\mu}m$ polyethylene bags and stored at $5^{\circ}C$ for 10 days. Off-odor of packaged baby leaves was not detected during storage. There was no significant difference in color parameters among the treatments. Samples treated with $O_3$ showed substantially higher electrolyte leakage throughout the storage. This treatment also rendered a higher accumulation of $CO_2$ in the packages. Samples treated with Cl and CA+Et maintained good overall visual quality with higher scores compared to that of $O_3$ and $ClO_2$. Although Cl treatment showed lower number of total aerobic count at the beginning of storage, citric acid in combination with ethanol treatment was more effective until the end of storage. The combined treatment also showed comparatively lower coliform plate count. This result indicates that citric acid wash followed by ethanol spray could be an alternative to chlorine for environment friendly sanitization of baby leaves.

• 대한조선학회논문집
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• 제35권1호
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• pp.88-97
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• 1998

파형 심재를 가지는 샌드위치 평판은 종 횡방향으로 충분한 강도를 가지며, 부재를 연결하기 위한 용접라인이 감소되고, 종 부재를 보강하기 위한 횡부재가 필요하지 않는다. 이러한 특성을 활용한 대형 알루미늄 압출재는 철도 차량과 초고속선의 중량 및 생산비용 절감을 위하여 사용될 수 있다. 그러나, 대형 알루미늄 압출판의 적절한 설계를 위해서는 압출재의 구조적인 거동에 대한 이해가 선행되어야 한다. 본 논문에서는 먼저, 구조적인 거동을 이해하기 위한 상세 유한요소 해석을 수행하였고 둘째로, 설계에 적용하기 위한 간략화된 이론식을 제안하였다. 제안된 간략식은 상세 유한요소 해석에 의한 결과와 비교해 볼 때 높은 정확도를 보이고 있음을 알 수 있었고, 제안된 간략식은 초기 설계 단계에서의 반복적인 해석에서 유용하게 사용될 수 있을 것이다.

• 대한구순구개열학회지
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• 제3권2호
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• pp.41-50
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• 2000

The alar base on the cleft side in unilateral complete cleft lip, alveolus and palate is markedly displaced laterally, caudally and dorsally, By incising the pyriform margin from the cleft margin of the alveolar process, including mucosa of the anterior part of the inferior turbinate, to the upper end of the postnasal vestibular fold, the alar base is released from the maxilla, A physiological correction of nasal deformity can be accomplished by careful reconstruction of nasolabial muscle integrity, functional repair of the orbicular muscle, raising and rotating the displaced alar cartilage, and finally by lining the lateral nasal vestibule, The inferior maxillary head of the nasal muscle complex is identified as the deeper muscle just below the web of the nostril, The muscle is repositioned inframedially, so that it is sutured to the periosteum that overlies the facial aspect of the premaxilla in the region of the developing lateral incisor tooth, And then, the deep superior part of the orbicular muscle is sutured to the periosteum and the fibrous tissue at the base of the septum, just in front of the anterior nasal spine, The nasal floor is surgically created by insertions of the nasal muscle complex in deep plane and of the orbicular muscle in superficial one, The upper part of the lateral nasal vestibular defect is sutured by shifting the alar flap cephalically, The middle and lower parts of this defect are closed by use of cleft margin flaps of the philtral and lateral segments, respectively, Authors stress the importance of nasal floor reconstruction at primary surgery and report the technique and postoperative results.

• 한국추진공학회지
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• 제17권6호
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• pp.75-80
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• 2013

본 연구에서는 마하 2의 실험실 규모의 풍동장치에서 플라즈마 제트 토치를 점화기로 사용하여 강제점화에 대한 연소 특성을 연구하였다. 하이퍼 혼합기는 혼합기로 사용되었다. 수직분사의 경우, 하나는 하이퍼 혼합기의 웨지면에 충돌하도록 하였으며, 다른 하나는 차가운 주유동으로 바로 분사되도록 하였다. 하이퍼 혼합기와 충돌하는 경우 충돌된 연료는 분산되며 확산 혼합에 의해 혼합성능이 증대된다. 또한 혼합된 가스는 대부분 플라즈마 제트의 열원으로 유입되어 연소 성능을 증대시킨다. 하지만 주유동으로 직접 분사되는 경우는 초음속의 주유동 내에서 점화되지 못하고 많은 양의 연료가 소비된다. 따라서 강제점화방식의 연소의 경우에는 많은 양의 연료-공기 혼합물을 점화가 가능한 열원으로 공급하는 것이 중요하다.

• 한국수소및신에너지학회논문집
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• 제23권5호
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• pp.468-475
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• 2012

A proper stacking force and assembly are important to the performance of fuel cell. Improper assembly pressure may lead to leakage of fuels and high interfacial contact resistance, excessive assembly pressure may result in damage to the gas diffusion layer and other components. The pressure distribution of gas diffusion layer is important to make interfacial contact resistance less for stack performance. To analyze the influence of design parameter factors for pressure distribution, and to optimize stack design, DOE (Design of Experiment) was used for polymer electrolyte membrane fuel cell stack pressure test. As commonly known, the higher clamping force improves the fuel cell stack performance. However, non-uniformity of stress distribution is also increased. It shows that optimization between clamping force and stress distribution is needed for well designed structure of fuel cell stack. In this study, stack design optimization method is suggested by using FEM (Finite Element Methode) and DOE for light-weighted fuel cell stack.

• 한국항공우주학회지
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• 제44권1호
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• pp.1-11
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• 2016

큰 가로세로비를 가지는 유연날개의 변형정보는 구조 건전성 평가를 위한 실시간 모니터링에 필요하다. 비행 중인 날개 구조 대변형은 날개 외피의 변형률과 곡률의 관계식을 기반으로 한 비선형 변위 예측 알고리즘을 통해 예측될 수 있다. 그러나 동체에 고정된 날개의 기하학적인 형상으로 인하여 고정단 부근에서의 변형률 분포는 복잡한 양상을 나타내며, 변형률 센서가 부착된 센싱라인의 코드방향 위치에 따라 변위가 다르게 예측될 수 있다. 본 논문에서는 스팬방향 변형률의 보정을 통하여 변형률 센싱라인의 코드방향 위치에 관계없이 예측변위의 정확도를 향상시키는 연구를 수행하였다. 변형률 보정을 위하여 스팬방향 및 코드방향 변형률의 비, 재료의 포아송비, 보와 평판 모델의 변형률 비를 이용하였다. 보정된 변형률을 이용하여 예측한 변위는 해석변위와 잘 일치하였으며, 이를 실험을 통하여 검증하였다.