• Journal of Microbiology and Biotechnology
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• v.28 no.10
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• pp.1749-1759
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• 2018

Recombinant (rec) prion protein (PrP) is an extremely useful resource for studying protein misfolding and subsequent protein aggregation events. Here, we report mass production of high-purity rec-polypeptide encoding the C-terminal globular domain of PrP; (90-230) for human and (89-231) for murine PrP. These proteins were expressed as His-tagged fusion proteins in E. coli cultured by a high cell-density aerobic fermentation method. RecPrPs recovered from inclusion bodies were slowly refolded under reducing conditions. Purification was performed by a sequence of metal-affinity, cation-exchange, and reverse-phase chromatography. The current procedure yielded several dozens of milligrams of recPrP per liter with >95% purity. The purified recPrPs predominantly adopted an ${\alpha}$-helix-rich conformation and were functionally sufficient as substrates to measure the seeding activity of human and animal prions. Establishment of a procedure for high-level production of high-purity recPrP supports the advancement of in vitro investigations of PrP including diagnosis for prion diseases.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.28 no.1C
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• pp.1-8
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• 2003

In this paper, performance of the CPC(complex phase code) which is recently proposed as a practical phase encoding method for phase-code multiplexes holographic memory system is comparatively analyzed with those of the conventional phase codes such as PR(pure random code), RCE(random code with equality), WHM(Walsh Hadamard Matrix). In computer simulation, the size of an address bean is fixed at 32$\times$32 pixels and 0%-25% phase-error ratio in a pixel are intentionally added to the real phase values to consider the nonlinear phase-modulation characteristics of the practical spatial light modulator. From comparative analysis of crosstalks and signal-to-noise ratios for these phase codes by calculating auto-correlation and cross-correlation, it is found that the CPC have the lowest cross-correlation mean value of 0.021, the lowest standard deviation of 0.0113 and the highest signal-to-noise ratio(SNR) of 27.4 among the four types of phase code. In addition, from the calculation of the number of all possible address beams for these four types of phase code as the size of the address beam is fixed to 3232 pixels, the CPC is found to have 6.334$\times$10$^{49}$ address beams, which are relatively higher number than that of the conventional phase codes.

• Korean Security Journal
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• no.23
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• pp.87-107
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• 2010

The purpose of this study is to examine the effects of customer aggression on the job stress and emotional burnout of security guards in casino. Investigating tools used questionnaire, the sampling was drawn from security guards at 6 casinos which had more than 30 security guards. To this study, the partisans of the study were 269 people. Data for creation for questionnaire is completely computed processing and using SPSS 15.0 Windows that is social studies scholastic mantle command program after imputed encoding according to each purpose. Analyzing techniques to use for analysis of data enforced technology figure analysis, factor analysis, reliability analysis, t-test, one-way ANOVA, multiple regression analysis. Through data analysis by such method and procedure, conclusion that gets in this research is as following. First, exposure that is difference that keep in mind partially as result that verify difference of aggression according to demographical features, job stress and emotional burnout that is security guard's in casino. Second, exposure that is difference that keep in mind as statistical as result that verify effect that customer aggression gets in job stress of security guards in casino. Third, exposure that is difference that keep in mind as statistical as result that verify effect that customer aggression gets in emotional burnout of security guards in casino.

• Korean Journal of Veterinary Research
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• v.36 no.1
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• pp.109-118
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• 1996

The Salmonella rfb gene encoding for the biosynthesis of the oligosaccharide-repeating units of the O-antigenic determinants was cloned and sequenced. A set of nucleotide primers(a forward and reverse) was selected to target a defined region of the guanosine diphospho-mannose(GDP-Man) pyrophosphorylase synthase gene : rfbM of Salmonella C serogroup. The primer set was used to develop a PCR-based rapid and specific detection system for Salmonella C1 serogroup. Amplification bands of predicted size(1,422bp) were generated from 11 different Salmonella C1 isolates. The bands were verified to be specific for the C1 serogroup by Southern blot analysis using reference homologous DNA specificity was further confirmed by the lack of reactivity with heterologous DNA derived from non-salmonella members of the family enterobacteriaeceae. A specificity of 100% was deduced along with a very high sensitivity shown by a detection limit of 1fg of a purified DNA template. The isolated DNA sequence was found to be 99.8% homologous to S montevideo but the related primers amplified with the predicted band sizes with all the Salmonella C1 serogroups tested. It is concluded that the PCR protocol based on the rfbM gene from S cholerasuis is optimal fast and specific for the detection of Salmonella C1 serogroup and also the corresponding probe is suitable for rapid detection of all Salmonella C1 serogroup DNA tested. This technology should facilitate the identification of contaminated pig products and for any other products contaminated with the Salmonalla C1 serogroup. The immediate impact of this developed method will be in the area of food safety of pig products with the potential prospect for adaptation to other food inspection technologies.

• Korean Journal of Microbiology
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• v.35 no.1
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• pp.7-12
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• 1999

By SDS-polyacrylamide gel elcctrophoresis (SDS-PAGE) and immunoblot analysis that a protein with 66,000 daltons in size was recognized by P. aeruginosa anti-exotoxin A from P. sp. PY002. The yields of exotoxin A in P. sp. PY002 culture were influenced by the concentration of iron in the culture media. Increasing of the exotoxin A production and siderophore production was made slight increasing in the MKB medium. On the other hand, to clone the gene encoding the exoloxin A genomic library of P. sp. PY002 was constructed in pBluescript SK(+). From this library a exotoxin A homologous gene was isolated by immunological hybridization method using P. aemginosa anti-exoloxin A as a probe. Two putative clones were isolated and designated pETA23 and pETA42. Thc restriction analysis ol pETA42 demonstrated that thc 1760 bp insert contained one NcoI, PvuII, SstI, Kpnl and EcoRI site and three SmaI and HaeD sites.

• Journal of Applied Biological Chemistry
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• v.58 no.3
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• pp.273-279
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• 2015

Phaeodactylum tricornutum is a model diatom that its genomic information and biological tools are well established. In this study, a gene encoding (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (PtHDR), a terminal enzyme of the methylerythritol phosphate pathway regulating chlorophyll and carotenoid biosynthesis, was isolated from P. tricornutum. The isolated gene was cloned into pPha-T1 vector containing fcpA promoter to prepare pPha-T1-HDR plasmid. As a positive control, pPha-T1-eGFP plasmid was constructed with egfp gene. Stable nuclear transformation was carried out with these plasmids by particle bombardment method and zeocin resistant colonies of P. tricornutum were selected on f/2 agar plate. In result, transformation efficiency was evaluated according to the amount of plasmid DNA coated with gold particles. Integration of introduced plasmids was confirmed with genomic DNA of each transformant by polymerase chain reaction. The eGFP fluorescence was visible in the cytoplasm, indicating that eGFP was successively expressed in P. tricornutum system. The transcript level of exogenous Pthdr gene was evaluated with the obtained transformants. The results presented here demonstrated that introduction of Pthdr gene into P. tricornutum chromosome succeeded and expression of PtHDR was enhanced under the fcpA promoter.

• The Korean Journal of Food And Nutrition
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• v.9 no.4
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• pp.452-458
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• 1996

A PPIase gene of Bacillus stearothermophilus was screened from a genomic library by plaque hybridization using the A-1 primer as a probe. A PPIase positive plaque contained a 3.0kb insert of the chromosomal DNA. A 3.0kb fragment was subcloned into pUC18, resulting pPI1-40. A DNA fragment encoding the N-terminal portion of the PPIase in pPi-40 was amplified by polymerase chain reaction(PCR) method using the A-1 and B-2 primers. The amplified fragment was cloned into the Sma I site of pUC18 and recombinant plasmid was designated as pSN-18. The nucleotide sequence of 167bp fragment was determined. The deduced amino acid sequence of PPIase was completely matched with the determined N-terminal amino acid sequence of PPIase B. stearothermophilus. The translated protein sequence of PPIase B. stearothermophilus was compared with sequence from periplasmic PPIase from Escherichina coil ; homogies of 16 and 58%, respectively, were found. The clond PPIase gene was over-expressed in E. coil cell using pUC19 as an expression vector. The enzyme was partially purified by heat treatment and colum chromatochraphy on DEAE-Sepharose CL-6B. The molecular weight of the enzyme was dermined to be about 18.0 kDal by SDS-PAGE.

• Journal of Broadcast Engineering
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• v.15 no.2
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• pp.205-216
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• 2010

In this paper, a method of MPEG-2 Transport Stream (TS) multiplexing for Ultra HDTV (UHDTV) and its design and implementation as a SW tool is described. In practice, UHD video may be divided into several HD videos and each video is encoded in parallel. Therefore, it is necessary to synchronize and multiplex multiple bitstreams encoding each HD video for transmitting and storing UHD video. In this paper, it is assumed that 4 HD videos partitioning a UHD spatially are encoded as H.264/AVC and two 5.0 channel audios are encoded by AC-3. Therefore, 4 H.264/AVC elementary streams (ESs) and 2 AC-3 ESs is mainly considered in the TS multiplexing of UHD. For the carriage of H.264/AVC and AC-3 over MPEG-2 TS, PES packetization and TS multiplexing are designed and implemented based on the extended specification of the MPEG-2 Systems and ATSC (Digital audio compressed standard), respectively. The implemented UHD TS multiplexing tool emulates real time HW operation in the time unit corresponding to the duration of one TS packet transmission in a given TS rate. In particular, in order to satisfy the timing model, the buffers defined in the TS System Target Decoder (T-STD) are monitored and their statuses are considered in the scheduling of TS multiplexing. For UHD multiplexing, two kinds of multiplexing structures, which are UHD re-multiplexing and UHD program multiplexing, are implemented and their strength and weakness are investigated. The developed UHD TS multiplexing tool is tested and verified in terms of the syntax and semantics conformance and functionalities by using a commercial analyzer and real-time presentation tools.

• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.616-625
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• 2005

It is likely that maltose could provide a good substrate for the bacteria in the intestine, when the pathogenic bacteria invade and colonize in human gut. For better understanding of this organism's maltose metabolism, a mutant that was not able to grow with maltose as a sole carbon source was screened from a library of mutants constructed by a random transposon mutagenesis. By a transposon-tagging method, malPQ genes encoding a maltodextrin phosphorylase and a 4-${\alpha}$-glucanotransferase, were identified and cloned from Vibrio vulnificus. The deduced amino acid sequences of malPQ from V. vulnificus were 48 to $91\%$ similar to those of MalP and MalQ reported from other Enterobacteriaceae. Functions of malPQ genes were assessed by the construction of mutants whose malPQ genes were inactivated by allelic exchanges. When maltose was used as the sole carbon source, neither malP nor malQ mutant was able to grow to a substantial level, revealing that the MalP and MalQ are the only enzymes for metabolic utilization of maltose. The malQ mutant exhibited decreased adherence toward intestinal epithelial cells in vitro, but there was no difference in the $LD_{50}s$ of the wild-type and the malQ mutant in mice. Therefore, it appears that MalQ is less important in the pathogenesis of V. vulnificus than would have been predicted by considering maltose as a most common sugar in the intestine, but not completely dispensable for virulence in mice.

• Journal of KIISE:Computing Practices and Letters
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• v.7 no.2
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• pp.165-182
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• 2001

In this paper, we describe a design and implementation of the Wireless Markup Language(WML) compiler to deploy wireless Internet services effectively. The WML compiler translates textual WML decks into binary ones in order to reduce the traffic on wireless links that have relatively low bandwidth to wireline links and mitigate the processing overhead of WML decks on, wireless terminals that have relatively low processing power to fixed workstations. In addition, it takes over the overhead of eXtensible Markup Language(XML) well-formedness and validation processes. The WML compiler consists of the lexical analyzer and parser modules. The granunar for the WML parser module is LALR(1) context-free grammar that is designed based on XML 1.0 and WML 1.2 DTD(Document Type Definition) with the consideration of the Wireless Application Protocol Binary XML grammar. The grammar description is converted into a C program to parse that grammar by using parser generator. Even though the tags in WML will be extended or WML DTD will be upgraded, this approach has the advantage of flexibility because the program is generated by modifying just the changed parts. We have verified the functionality of the WML compiler by using a WML decompiler in the public domain and by using the Nokia WAP Toolkit as a WAP client. To measurethe compressibility gain of the WML compiler, we have tested a large number of textual WML decks and obtained a maximum 85 %. As the effect of compression is reduced when the portion of general textual strings increases relative to one of the tags and attributes in a WML deck, an extended encoding method might be needed for specific applications such as compiling of the WML decks to which the Hyper Text Markup Language document is translated dynamically.