• 한국동물위생학회지
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• 제31권3호
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• pp.273-281
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• 2008

This survey was conducted to investigate the infection of caprine arthritis encephalitis virus (CAEV). From April to October 2007, we examined a total of 173 goats in 7 dairy goat-breeding farms located in Na-ju, Hae-nam, Young-am, and Young-gwang area of Jeonnam province. The results show that 41 (23.7%) goats are positive for CAEV, confirmed by nested PCR of 173 blood samples. Regional analysis revealed that a positive proportion for CAEV was 50.0% in Young-gwang, 25.6% in Young-am, 25.0% in Hae-nam, and 10.9% in Na-ju. Clinical signs were observed in 17 (9.8%) goats, of which 6 suffered from arthritis, 7 from mastitis, and 4 from pneumonia. Among the examined 173 goats, 8(4.6%) goats are positive for CAEV accompanied with CAE symptoms. There was a tendency to be much higher(p<0.05) levels of natrium(Na), chlorine(Cl) in CAEV-positive than in CAEV-negative goats. However, other serum biochemical values were no statistically significant effect.

• 미생물학회지
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• 제38권3호
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• pp.221-229
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• 2002

일본뇌염바이러스(Japanese encephalitis virus, JEV) Nakayama strain을 초기 multiplicity of infection 5.0으로 Vero세포에 감염하여 1년 이상 안정적으로 바이러스를 방출하는 지속감염(persistently-infected)세포주를 확립하였다. 지속감염 세포에서 지속적으로 방출되는 총 11 개의 Plaque 형태 변이바이러스(morphology mutants)클론을 확보하였다. 분리된 변이바이러스의 복제효율을 분석한 결과 생물학적 표현형과 복제효율은 유의하게 상관하였다. 변이바이러스 RNA 게놈 양 말단의 non-coding region 및 envelop 단백질의 ORF에서는 유의한 염기서열 변화가 관찰되지 않아 JEV 약독화에 새로운 인자가 추가로 관여할 가능성을 제시하였다. 변이바이러스에 감염된 신선한 Vero세포는 wild-type JEV의 일반적 감염성상과 다르게 대다수의 세포가 유의할 만한 세포병변현상을 나타내지 않았다. 감염된 Vero세포에서 wild-type JEV 및 large plaque을 형성하는 변이바이러스의 경우 mRNA와 함께 Bcl-2의 발현은 모두 유의하게 감소하였으며 p53은 뚜렷하게 증가하였다. 반면 small plaque을 형성하는 변이바이러스의 감염세포에서는 Bcl-2와 p53 모두 유의한 변화를 볼 수 없었다. 이상의 결과들과 함께, 감염된 Vero세포의 internucleosomal DNA fragmentation과DNA profile의 유형분석에 따르면 궁극적 인 세포병변효과의 변화는 변이바이러스의 복제효율과 더불어 p53에 비의존적인 apoptosis 수위의 전반적인 감소에 기인하는 것으로 판단된다.

• 한국수의병리학회지
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• 제7권1호
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• pp.23-26
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• 2003

Newcastle disease (ND) is acute respiratory disease with high mortality in chicken and pet birds. Two ostriches aged on 51 days old submitted had showed clinical signs of severe torticolis and poor locomotion. In gross, fibrinous air-sacculitis and hemorrhagic enteritis were present. The significant lesions were histopathologically lymphocytic encephalitis such as perivascular cuffings and gliosis in neuropil. The velogenic ND virus was isolated ftom 10 day-old embryonated eggs inoculated with hemogenized cecal tonsils. These results suggest that NO occurred in the ostrich in Korea.

• 미생물학회지
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• 제46권2호
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• pp.140-147
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• 2010

일본뇌염바이러스(Japanese encephalitis virus)는 모기 매개성 플라비바이러스에 속하며, 주로 동남아시아 지역에서 유행성 바이러스성 뇌염을 일으킨다. 일본뇌염바이러스는 외피를 가진 작은 바이러스로서, 양성가닥 RNA 게놈을 가지고 있다. 감염성을 띤 바이러스 입자는 capsid (C), membrane (M; prM 전구체로부터 생성), 및 envelope (E)과 같은 3개의 구조단백질로 이루어져 있다. 본 연구에서는 일본뇌염바이러스 생산과 정에 M 단백질의 N-말단부위에 위치한 극성 아미노산 잔기의 역할을 분석하였다. 일본뇌염바이러스의 infectious cDNA를 활용하여, M 단백질의 $E^9$와 $K^{15}K^{16}E^{17}$ 잔기를 알라닌으로 치환시킨 2개의 mutant cDNA (Mm1과 Mm2)를 각각 제작하였다. 각각의 cDNA로부터 합성된 mutant RNA를 세포에 트랜스펙션시킴으로써, 비록 세포 내에 축적된 3개의 구조단백질양은 변화가 없으나, 이들 세포로부터 생산된 바이러스의 양은 Mm2 RNA의 경우 ~1,000배 감소됨을 관찰하였다. 흥미롭게도, Mm2 RNA로부터 발현된 mutant M 단백질은 wild-type M 단백질을 인지하는 항혈청에는 반응하지 않았으나, mutant M 단백질을 항원으로 제작된 항혈청에는 반응하는 것을 알 수 있었다. 본 실험결과는 일본뇌염바이러스 M 단백질을 구성하는 3개의 극성 아미노산 잔기($K^{15}K^{16}E^{17}$)가 바이러스의 생산과정에 관여한다는 것을 암시한다. 앞으로, wild-type 또는 mutant M 단백질(Mm2)을 인식하는 2개의 항혈청은 이 단백질의 기능연구에 유용한 재료로 사용될 것으로 기대된다.

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2005년도 International Meeting of the Microbiological Society of Korea
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• pp.211.1-211
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• 2005

• 한국미생물학회:학술대회논문집
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• 한국미생물학회 2003년도 International Meeting of the Microbiological Society of Korea
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• pp.83-86
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• 2003

Major advances in positive-sense RNA virus research have been facilitated by the development of reverse genetics systems. These systems consist of an infectious cDNA clone that encompasses the genome of the virus in question. This clone is then used as a template for the subsequent synthesis of infectious RNA for the generation of synthetic viruses. However, the construction of infectious cDNA for the Japanese encephalitis virus (JEV) has been repeatedly thwarted by the instability of its cDNA. As JEV is an important human pathogen that causes permanent neuropsychiatric sequelae and even fatal disease, a reliable reverse genetics system for this virus is highly desirable. The availability of this tool would greatly and the development of effective vaccines as well as facilitate studies into the basic biology of the virus, including the molecular mechanisms of viral replication, neurovirulence, and pathogenesis. We have successfully constructed a genetically stable infectious JEV cDNA containing full-length viral RNA genome. Synthetic RNA transcripts generated in vitro from the cDNA were highly infectious upon transfection into susceptible cells, and the cDNA remained stable after it had been propagated in E. coli for 180 generations. Using this infectious JEV cDNA, we have successfully expressed a variety of reporter genes from the full-length genomic and various subgenomic RNAs in vitro transcribed from functional JEV cDNAS. In summary, we have developed a reverse genetics system for JEV that will greatly facilitate the research on this virus in a variety of different fields. It will also be useful as a heterologous gene expression vector and aid the development of a vaccine against JEV.

• 대한수의학회지
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• 제11권2호
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• pp.163-170
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• 1971

Since the report in 1946 on the first isolation of Japanese encephalitis virus in Korea, the disease is known to occur every year and has occasionally spread in epidemic proportion among human populations. Because of its public health importance, it was deemed highly desirable to gain specific information as to natural history of the disease in Korea if we are to accomplish our ultimate objective of controling the disease in Korea. There still remain, however, unknown factors as to the ecology of Japanese encephalitis virus in Korea. This paper presents the results of serologic study on Korean cattle, hog and horse by means of hemagglutination inhibition (H.I.) test. The serum specimens were collected from Korean cattle, hog and horse their estimated ages being 3 to 9 years, 6 to 24 months and 2 to 14 years respectively, by jugular puncture; in Seoul and Kyung-Ki are a from the first part of May to November last, 1968. The strain used was designated by M 5/596. The methods of H.I. test employed in this study were essentially simillar to those employed by Clark and Fred. The results obtained summarized as follows; 1. Of samples of cattle sera tested, 183 (98%) was found to be positive, H.I. antibodies showing the highest proportion, on September. The lowest proportion showed 81 (68%) of 117 samples, on June. 2. One hundred and ninety-five and 114 swine serum samples tested were all of positive in both on September and October respectively. The lowest proportion showed 92 (29%) of 314 on June too. 3. Ninety (99%) of 91 equine serum samples tested contained demonstrable H.I. antibodies the highest proportion, on August. The lowest proportion was 19 (27%) of 70 samples on June. 4. Implications of these findings in the ecology of Japanese encephalitis Seoul and Kyung-Ki area were discussed.

• Toxicological Research
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• 제13권3호
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• pp.281-291
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• 1997

The subacute toxicity of the combined vaccine (KGCC-95VI) for the prophylaxis against Japanese encephalitis and Hantaan virus infection, recently developed by Korea Green Cross Corporation, was investigated. KGCC-95VI was subcutaneously administered into the both sexes of New Zealand White rabbits at the dosage of 0, 10. 50 and 250 ml/kg body weight (20, 100 and 500 times the expected clincal dose) once a day for 30 days. There were no deaths and clinical findings during the experiment period. In both sexes. there were no statistically significant differences between the treated and control groups in urinalysis tests, hematological tests, blood chemistry tests and pathological examinations. The KGCC-95VI is considered not to have the subacute toxicity in the rabbits.

• 대한핵의학회지
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• 제23권1호
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• pp.85-87
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• 1989

Herpes simplex encephalitis (HSE) is one of the fulminant necrotizing, often fatal sporadic form of the encephalitis caused by herpes simplex type I virus. Characteristically, there is early and almost constant involvement of one or both temporal lobes, although there are common additional areas of involvement. Appropriate early treatment following correct diagnosis by clinical findings, CSF study, EEG and several radiological studies including angiography, redionuclide studies, CT or MRI can reduce its mortality and severity of the sequelae. We report a case of HSE diagnosed by adjuvant study of $^{99m}Tc-HMPAO$ regional cerebral blood flow SPECT, which showed a marked increase in bitemporal cerebral blood flow in a 24-year-old man.

• Annals of Clinical Neurophysiology
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• 제16권1호
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• pp.35-38
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• 2014

Japanese-B viral encephalitis (JE) usually has a monophasic illness pattern. A 45-year-old woman in an altered mentality had improved over 1 month. About 1 week after the initial improvement, the patient became comatose with aggravated EEG and MRI findings. Assays of cerebrospinal fluid and serum were positive for the IgM antibody to Japanese-B virus. After intravenous immunoglobulin (IVIG) infusion, the patient recovered. We report a patient with JE who showed a biphasic illness pattern and recovered after IVIG therapy.