• Journal of Microbiology and Biotechnology
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• v.15 no.3
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• pp.525-531
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• 2005

From a genomic library of Lactobacillus reuteri ATCC 55739, one clone, NE347, carrying a pyrH gene encoding UMP kinase, was identified. pNE347 carried a 1.88 kb EcoRI fragment and the pyrH was located in the middle of the insert. pyrH ORF was 723 bp in size and capable of encoding UMP kinase composed of 240 amino acid residues. tsf encoding an elongation factor-Ts and frr encoding a ribosomal recycling factor were present upstream and downstream of pyrH, respectively. When introduced into E. coli KUR1244, a pyrH-negative strain, pNE347 restored the ability to grow at $42^{\circ}C$, indicating that pyrH from L. reuteri synthesized functional UMP kinase in E. coli. Northern blot experiment showed that pyrH and frr were cotranscribed as a 1.4 kb single transcript. pyrH was overexpressed in E. coli by using a pET26b(+) vector, and a major 25 kDa protein band appeared on SDS-polyacrylamide gel.

• Journal of the Microelectronics and Packaging Society
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• v.26 no.4
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• pp.47-53
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• 2019

Stiffness-gradient stretchable electronic packages of the soft PDMS/hard PDMS/PTFE structure were processed using the polydimethylsiloxane (PDMS) as the base substrate and the more stiff polytetrafluoroethylene (PTFE) as the island substrate, and their stretchable deformation-resistance characteristics were characterized. The flip-chip joints, formed by bonding the chip bumps of 50 ㎛-diameter onto the PDMS/PTFE substrate pads, exhibited an average contact resistance of 96 mΩ. When the stretchable package of the soft PDMS/hard PDMS/PTFE structure was deformed to 30% elongation, the strain on the PTFE was restrained to 1%, resulting in a negligible resistance increase of 1% in the daisy-chain circuit formed on the PTFE island substrate. The circuit resistance increased for 1.7% after 2,500 cycles of 0~30% stretchable deformation.

• Journal of Korea Foundry Society
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• v.37 no.5
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• pp.139-147
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• 2017

Microstructural evolution of cast Hastelloy X during thermal exposure has been investigated. OM, SEM, and TEM microscopy were carried out on the as-cast, the standard heat treated, and the thermally exposed conditions. Tensile tests were also conducted to understand the effect of microstructural evolution on the degradation of tensile properties. Coarse $M_6C$ and fine $M_{23}C_6$ carbides were found in as-cast Hastelloy X with fine carbides on sub-boundary. Some of $M_{23}C_6$ carbide dissolved into the matrix during solution heat treatment and dislocation network formed at the interface between the carbide and the matrix due to the misfit strain. There was no significant microstructural difference between the exposed specimens at $400^{\circ}C$ and the solution heat treated specimen. A large amount of $M_{23}C_6$ carbides precipitated along and near grain boundaries and sub-boundaries after exposure at $650^{\circ}C$. Exposure at $870^{\circ}C$ of the alloy caused precipitation of $M_6C$ and ${\mu}$. The strength increased and the elongation decreased by thermal exposure at $650^{\circ}C$ and $870^{\circ}C$ because carbides interfere with the movement of the dislocation. It was found that the precipitation of carbide gave significant effects on the tensile properties of Hastelloy X.

• Transactions of Materials Processing
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• v.21 no.1
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• pp.49-57
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• 2012

It is difficult to estimate the properties of multilayered sheet because they are composed of one or more different materials. Plastic deformation behavior of the multilayered sheet is quite different as compared to each material individually. The deformation behavior of multilayered sheet should be investigated in order to prevent forming defects and to predict the properties of the formed part. In this study, the mechanical properties and formability of stainless steel-aluminum-magnesium multilayered sheet were investigated. The multilayered sheet needs to be deformed at an elevated temperature because of its poor formability at room temperature. Uniaxial tensile tests were performed at various temperatures and strain rates. Fracture patterns changed mainly at a temperature of $200^{\circ}C$. Uniform and total elongation of multilayered sheet increased to values greater than those of each material when deformed at $250^{\circ}C$. The limiting drawing ratio (LDR) was obtained using a circular cup deep drawing test to measure the formability of the multilayered sheet. A maximum value for the LDR of about 2 was achieved at $250^{\circ}C$, which is the appropriate forming temperature for the Mg alloy. Fracture patterns on a circular cup and thickness of formed part were predicted by a rigid-viscoplastic FEM analysis. Two kinds of modeling techniques were used to simulate deep drawing process of multilayered sheet. A single-layer FE-model, which combines the three different layers into a macroscopic single layer, predicted well the thickness distribution of the drawn cup. In contrast, the location and the time of fracture were estimated better with a multi-layer FE model, which used different material properties for each of the three layers.

• Journal of Ocean Engineering and Technology
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• v.33 no.3
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• pp.259-271
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• 2019

It is important to obtain reasonable predictions of the extent of the damage during maritime accidents such as ship collisions and groundings. Many fracture models based on different mechanical backgrounds have been proposed and can be used to estimate the extent of damage involving ductile fracture. The goal of this study was to compare the damage extents provided by some selected fracture models. Instead of performing a new series of material constant calibration tests, the fracture test results for the ship building steel EH36 obtained by Park et al. (2019) were used which included specimens with different geometries such as central hole, pure shear, and notched tensile specimens. The test results were compared with seven ductile fracture surfaces: Johnson-Cook, Cockcroft-Latham-Oh, Bai-Wierzbicki, Modified Mohr-Coulomb, Lou-Huh, Maximum shear stress, and Hosford-Coulomb. The linear damage accumulation law was applied to consider the effect of the loading path on each fracture surface. The Swift-Voce combined constitutive model was used to accurately define the flow stress in a large strain region. The reliability of these simulations was verified by the good agreement between the axial tension force elongation relations captured from the tests and simulations without fracture assignment. The material constants corresponding to each fracture surface were calibrated using an optimization technique with the minimized object function of the residual sum of errors between the simulated and predicted stress triaxiality and load angle parameter values to fracture initiation. The reliabilities of the calibrated material constants of B-W, MMC, L-H, and HC were the best, whereas there was a high residual sum of errors in the case of the MMS, C-L-O, and J-C models. The most accurate fracture predictions for the fracture specimens were made by the B-W, MMC, L-H, and HC models.

• Food Engineering Progress
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• v.21 no.3
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• pp.233-241
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• 2017

Scaffolds of cell substrates are biophysical platforms for cell attachment, proliferation, and differentiation. They ultimately play a leading-edge role in the regeneration of tissues. Recent studies have shown the potential of bioactive scaffolds (i.e., osteo-inductive) through 3D printing. In this study, rice bran-derived biocomposite was fabricated for fused deposition modeling (FDM)-based 3D printing as a potential bone-graft analogue. Rice bran by-product was blended with poly caprolactone (PCL), a synthetic commercial biodegradable polymer. An extruder with extrusion process molding was adopted to manufacture the newly blended "green material." Processing conditions affected the performance of these blends. Bio-filament composite was characterized using field emission scanning electron microscopy (FE-SEM) and energy dispersive X-ray spectroscopy (EDX). Mechanical characterization of bio-filament composite was carried out to determine stress-strain and compressive strength. Biological behaviors of bio-filament composites were also investigated by assessing cell cytotoxicity and water contact angle. EDX results of bio-filament composites indicated the presence of organic compounds. These bio-filament composites were found to have higher tensile strength than conventional PCL filament. They exhibited positive response in cytotoxicity. Biological analysis revealed better compatibility of r-PCL with rice bran. Such rice bran blended bio-filament composite was found to have higher elongation and strength compared to control PCL.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.21 no.3
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• pp.12-21
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• 2022

Directed energy deposition (DED) was adopted as a metal additive manufacturing method to develop a mold for the hot stamping process. The test piece was machined from Heatvar laminate material, and results were obtained through microstructure and defect observations, as well as hardness, tensile strength, and joint strength tests. 1) Spherical pores and irregular-shaped cavities were observed as lamination defects, and columnar dendrites formed in the structure, which tended to become coarse upon heat treatment. 2) The hardness of the heat-treated material (480HV) was slightly lower than that of the non-heat-treated material (500HV). 3) In the tensile test, the maximum tensile stress and strain of the heat-treated material were 1392 MPa and 15%, respectively, which were slightly higher than the values of 1381 MPa and 13%, respectively, for the non-heat-treated material. 4) In the case of the early final fracture in the tensile test, in most cases, pores or irregularly shaped cavities were observed at the fracture surface or near the surface. 5) In the joint strength test, most of the specimens finally fractured in the laminated metal area, and the fracture surface was intragranular. In addition, dimples formed over the entire area on the fracture surface of the fractured specimen after sufficient elongation.

• Journal of Powder Materials
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• v.29 no.5
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• pp.382-389
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• 2022

A typical trade-off relationship exists between strength and elongation in face-centered cubic metals. Studies have recently been conducted to enhance strength without ductility reduction through surface-treatment-based ultrasonic nanocrystalline surface modification (UNSM), which creates a gradient microstructure in which grains become smaller from the inside to the surface. The transformation-induced plasticity effect in Fe-Mn alloys results in excellent strength and ductility due to their high work-hardening rate. This rate is achieved through strain-induced martensitic transformation when an alloy is plastically deformed. In this study, Fe-6%Mn powders with different sizes were prepared by high-energy ball milling and sintered through spark plasma sintering to produce Fe-6%Mn samples. A gradient microstructure was obtained by stacking the different-sized powders to achieve similar effects as those derived from UNSM. A compressive test was performed to investigate the mechanical properties, including the yielding behavior. The deformed microstructure was observed through electron backscatter diffraction to determine the effects of gradient plastic deformation.

• Steel and Composite Structures
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• v.47 no.2
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• pp.269-287
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• 2023

The WGJ420 fire-resistant weathering (FRW) steel is developed and manufactured with standard yield strength of 420 MPa at room temperature, which is expected to significantly enhance the performance of steel structures with excellent fire and corrosion resistances, strong seismic capacity, high strength and ductility, good resilience and robustness. In this paper, the mechanical properties of FRW steel plates and buckling behavior of columns are investigated through tests at elevated temperatures. The stress-strain curves, mechanical properties of FRW steel such as modulus of elasticity, proof strength, tensile strength, as well as corresponding reduction factors are obtained and discussed. The recommended constitutive model based on the Ramberg-Osgood relationship, as well as the relevant formulas for mechanical properties are proposed, which provide fundamental mechanical parameters and references. A total of 12 FRW steel welded I-section columns with different slenderness ratios and buckling load ratios are tested under standard fire to understand the global buckling behavior in-depth. The influences of boundary conditions on the buckling failure modes as well as the critical temperatures are also investigated. In addition, the temperature distributions at different sections/locations of the columns are obtained. It is found that the buckling deformation curve can be divided into four stages: initial expansion stage, stable stage, compression stage and failure stage. The fire test results concluded that the residual buckling capacities of FRW steel columns are substantially higher than the conventional steel columns at elevated temperatures. Furthermore, the numerical results show good agreement with the fire test results in terms of the critical temperature and maximum axial elongation. Finally, the critical temperatures between the numerical results and various code/standard curves (GB 51249, Eurocode 3, AS 4100, BS 5950 and AISC) are compared and verified both in the buckling resistance domain and in the temperature domain. It is demonstrated that the FRW steel columns have sufficient safety redundancy for fire resistance when they are designed according to current codes or standards.

• Journal of the Korean institute of surface engineering
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• v.56 no.2
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• pp.160-168
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• 2023

Recently, investigation on metallization is a key for a stretchable display. Amorphous metal such as Ni and Zr based amorphous metal compounds are introduced for a suitable material with superelastic property under certain stress condition. However, Ni and Zr based amorphous metals have too high resistivity for a display device's interconnectors. In addition, these metals are not suitable for display process chemicals. Therefore, we choose an aluminum based amprhous metal Al-Mo as a interconnector of stretchable display. In this paper, Amorphous Forming Composition Range (AFCR) for Al-Mo alloys are calculated by Midema's model, which is between 0.1 and 0.25 molybdenum, as confirmed by X-ray diffraction (XRD). The elongation tests revealed that amorphous Al-20Mo alloy thin films exhibit superior stretchability compared to pure Al thin films, with significantly less increase in resistivity at a 10% strain. This excellent resistance to hillock formation in the Al20Mo alloy is attributed to the recessed diffusion of aluminum atoms in the amorphous phase, rather than in the crystalline phase, as well as stress distribution and relaxation in the aluminum alloy. Furthermore, according to the AES depth profile analysis, the amorphous Al-Mo alloys are completely compatible with existing etching processes. The alloys exhibit fast etch rates, with a reasonable oxide layer thickness of 10 nm, and there is no diffusion of oxides in the matrix. This compatibility with existing etching processes is an important advantage for the industrial production of stretchable displays.