• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.331-336
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• 2010

This study was carried out to investigate the dose-dependent effect of methyl jasmonate on both the adventitious root growth and the accumulation of various eleutherosides in the bioreactor culture of Eleutherococcus senticosus adventitious roots. The highest biomass production (5.4 g DW/L) was observed in the absence of methyl jasmonate and the root growth was significantly decreased by increasing the methyl jasmonate concentration. However, methyl jasmonate stimulated the production of both eleutheroside B, E and $E_1$. The highest level of eleutheroside B (359.9 ${\mu}g$/g DW) was obtained at 40 ${\mu}M$ of methyl jasmonate, while eleutheroside E and $E_1$ was accumulated at the highest level by the addition of 10 ${\mu}M$ of methyl jasmonate. Total eleutheroside was increased up to 3818.1 ${\mu}g$ per liter when 10 ${\mu}M$ of methyl jasmonate was applied. In addition, when the adventitious roots were cultured with 20 ${\mu}M$ of methyl jasmonate, the highest levels of eleutheroside B, E and $E_1$ were observed at the 12th, 3th and 9th days of culture, respectively.

• Journal of Korean Society of Forest Science
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• v.100 no.2
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• pp.178-183
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• 2011

This study was carried out to investigate the dose-dependent effect of salicylic acid on both the adventitious root growth and the accumulation of various eleutherosides in the bioreactor culture of Eleutherococcus senticosus. The highest biomass production (5.4 g DW/L) was observed in the absence of salicylic acid, while the root growth was significantly decreased by increasing the concentration of salicylic acid. Salicylic acid stimulated the production of both eleutheroside B, E and $E_1$. The highest levels of eleutheroside B ($179.5{\mu}g/g$ DW), E ($1169.9{\mu}g/g$ DW) and $E_1$ ($45.4{\mu}g/g$ DW) were obtained by the addition of $80{\mu}M$ of salicylic acid. The maximum eleutheroside production was $4975.8{\mu}g/L$ when salicylic acid was not added. In addition, when the adventitious roots were cultured in the basal medium supplemented with $80{\mu}M$ of salicylic acid, the highest levels of eleutheroside B was observed at the 9th day, while eleutheroside E and $E_1$ were observed at the 6th day, respectively.