• 한국식품영양학회지
• /
• 제30권1호
• /
• pp.175-180
• /
• 2017

To enhance the physiological activities and reduce the off-flavor of garlic in the Kimchi, we manufactured the Kimchi seasoning replaced with 25~75% black garlic instead of raw garlic and measured the contents of antioxidants and antioxidant activities. The polyphenol compound content was $270.91{\mu}g/mL$ in the control and $278.18{\sim}305.75{\mu}g/mL$ in the Kimchi seasoning with black garlic. The polyphenol compound content increased by increasing the amount of black garlic in the Kimchi seasoning. The polyphenol compound contents gradually decreased during storage period. The flavonoid compound content was 26.18 mg% in the control and 26.80~38.72 mg% in the Kimchi seasoning replaced with 25~75% black garlic. The flavonoid compound contents increased by increasing the amount of black garlic. The flavonoid contents were different from those of polyphenol compounds during storage periods. In the case of DPPH electron donating abilities, DPPH electron donating ability of the control was 84.82%, which showed a lower antioxidative activity than the Kimchi seasoning replaced with black garlic. The electron donating abilities of the Kimchi seasoning replaced with black garlic were increased as the amount of black garlic was increased. In the lipid peroxidation test, the control showed no lipid peroxidation during the storage period. The Kimchi seasoning replaced with black garlic also inhibited lipid peroxidation during storage as in the control. However, it showed lower activity than the control. Reducing power was higher in the Kimchi seasoning replaced with black garlic than in the control. The higher the ratio of black garlic, the higher the reducing power.

• Fisheries and Aquatic Sciences
• /
• 제3권3_4호
• /
• pp.195-199
• /
• 2000

Tyrosinase inhibitory activities of 14 kinds of seaweed, Ecklonia stolonifera, Ecklonia cava, Undaria pinnatiflda, Laminaria japonica, Sargassum fulvellum, Sargassum miyabei, Sargassum thunbergii, Porphyra yezoensis, Gracilaria verrucosa, Carpopeltis affinis, Pachymeniopsis elliptica, Gelidium amansii, Codium fragile and Ulva pertusa were determined using commercially available mushroom tyrosinase in an in vitro assay system. The $1\%$ (w/v) methanol extract from E. stolonifera showed the highest tyrosinase inhibitory activity of $79.0\%$, electron donating activity of $79.0\%$ and total phenol content of 3.75 mg/100g. Ethyl acetate-methanol-water (7 : 2 : 0.2, v/v) fraction $(0.5\%,\;w/v)$ isolated from the methanol extract showed tyrosinase inhibitory activity of $75.9 \%$, electron donating activity of $88.1 \%$ and total phenol content of 4.38 mg/100g. Tyrosinase inhibitory activity was closely associated with total phenol content (R = 0.99) and electron donating activity (R=0.99). Maximum absorption wavelength of the fraction was 218nm and that of phenolic compounds showed about a range from 210 to 220nm. The inhibition mode of the fraction was noncompetitive inhibition.

• 농약과학회지
• /
• 제3권1호
• /
• pp.96-101
• /
• 1999

새로운 합성방법을 개발하여 pyrazolate 제초제의 곁가지를 변화시킨 새로운 구조의 3-trifluoro-methylpyrazole 유도체들과 4-benzenecarbinolpyrazole 유도체 등을 합성하여 전작과 답작상태에서 제초활성 시험을 하였다. 밭조건에서는 발아전처리의 경우 4 kg/ha의 약량에서도 제초효과를 나타내지 않았으며 발아후처리에서는 제초효과가 있었으나 미약하였다. 답작상태에서는 4 kg/ha의 약량에서 제초효과가 있었으며 특히 4-benzoylpyrazole의 benzene의 4위치에 electron-donating group인 methoxy가 치환된 화합물의 경우 활성과 선택성이 매우 좋았다. 그러나, methoxy가 두 개 이상 치환되거나 다른 electron-donating group인 sulfide기가 치환된 화합물들의 제초활성은 약하였다.

• 동의생리병리학회지
• /
• 제19권6호
• /
• pp.1580-1584
• /
• 2005

Antioxidative activities of catechin from green tea extracts were examined by the methods of 1,1-Diphenyl-2-picrylhydrazyl (DPPH) electron donating ability, hydroxy scavenging activity and the inhibitory effect on xanthine oxidase activity. The resulted demonstrated the fact that Catechin one (containing Green tea extracts plus catechin 51%) of green tea extracts product showed at 65.5% in electron donating activity on the DPPH. The electron donating ability on the DPPH of Catechin one was increased to 10% than purified catechin. Catechin one showed the activity at 64.5% in scavenging activity using hydroxy radical method. To the Catechin one provided to increase the hydroxy scavenging activity up to 3 fold. Inhibitory effects of the catechin one measured with xanthine oxidase method was 6.5%. Although the antioxidative activity of catechin (98% purified) was lower than that of Catechin one (containing Green tea extracts plus catechin 51%) in same catechin concentrations ($5{\mu}g$, respectively). Therefore, we may suggest that Catechin one can be used as a functional food additive possessing the potent antioxidative activity.

• 한국작물학회지
• /
• 제52권3호
• /
• pp.274-280
• /
• 2007

The effect of biofertilizer in enhancing nutrient quality and antioxidant property of rice grain was investigated. The experiment was carried out in a randomized complete block design with 3 replications and 7 treatments namely : RF = $N-P_2O_5-K_2O(11-5.5-4.8kg\;10a^{-1});$ half of the recommended fertilizer rate, $HRF=N-P_2O_5-K_2O(5.5-2.75-2.4kg\;10a^{-1}):$ HRF+Bio 250=HRF combined with 250 kg Biofertilizer 10 $a^{-1}$; HRF+Bio 500=HRF combined with 500 kg Biofertilizer 10 $a^{-1};$ Bio 250=250 kg Biofertilizer 10 $a^{-1};$ Bio 500=500 kg Biofertilizer 10 $a^{-1};$ and NF=No Fertilizer. Results showed that HRF+Bio 500 obtained a significantly higher protein content but a significantly lower amylose content compared with RF and NF treatments. Highest phytic acid content was recorded in NF treatment while the lowest was observed in HRF+500 treatment. The highest values in both electron donating ability and reducing power were obtained in HRF+Bio 500 treatment. All treatments obtained higher reducing power than that of the RF treatment and that NF treatment showed comparable values in both electron donating ability and reducing power with those of the treated plots. Highest antimutagenicity property was also observed in HRF+Bio 500 treatment followed by Bio 500 treatment. This study showed the possibility of using biofertilizer to enhance nutritional quality and antioxidant property of rice.

• 한국식품영양과학회지
• /
• 제30권4호
• /
• pp.717-720
• /
• 2001

4년근과 24년근 도라지의 화학성분 및 생리활성을 조사하였다. 24년근 장생 도라지의 화학성분 조성은 수분 82.7% 조단백 1.6% 조지방2.1%, 조회분 0.7% 총당 6.0% 총 식이섬유 2.3% 조사포닌 3.4mg% 및 비타민 C 3.0mg%였으며 4년근과의 함량차이는 크게 나타나지 않았다. 그러나 무기물의 함량은 24년근이 4년근에 비하여 모두 높게 나타났으며 그 중에서 K가 879.9mg/kg 으로 가장 높게 나타났다. 24년근과 4년근의 용매별 추출물의 생리활성 정도는 에탄올>메탄올>물의 순이었고 전자공여능은 24년근이 4년근보다 10-50% 높게 나타났으며 아질산업 소거효과도 10~40% 까지 높게 나타났다.

• 한국식품조리과학회:학술대회논문집
• /
• 한국식품조리과학회 2005년도 추계학술대회 및 정기총회
• /
• pp.56-65
• /
• 2005

This study was purposed to investigate the antioxidative effects, the enzyme activity of the alcohol metabolizing and melanin production of Maesil(Prune mume). The antioxidant activity of Maesil(Prunu mume) was analyzed by measuring thiobarbituric acid reactive substances(TBARS value) and electron donating ability. And we investigated the changes of alcohol dehydrogenase(ADH) and acetaldehyde dehydrogenase(ALDH) activity by measuring the maximum absorbency at 340nm in vitro and human study. The inhibitory effects of Maesil were investigated in vitro and in B-16mouse melanoma cells on melanin biosynthesis that is closely related to hyperpigmentation. The antioxidant activities for TBA values were 29.65% in ascorbicacid, 45.35% in BHT, 15.99% in extract of dehydrated maesil flesh(EDMF) and 25.00% in extract of dehydrated maesil juice(EDMJ). The electron donating abilities by DPPH were 96.69% in ascorbic acid, 77.82% in BHT, 34.25% in EDMF, and 42.99%in EDMJ. Electron donating abilities by DPPH in the presence of 0.02% EDMF and EDMJ were 53.21% and 59.19% respectively. Facilitating rates of ADH activity were 137.92, 131.58, 152.96, 218.70, 111.76, and 144.27% in maesil juice, 5, 10, and 15% GMT, and 0.5 and 1.0% aspartic acid, respectively. ALDH activity increased in the order of Maesil juice > ALDH > GMT > aspartic acid, and facilitating rate of ALDH activity in Maesil juice was the highest at 976.44%. Maesil extracts inhibited tyrosinase activity that converts dopa to dopachrome in the biosynthesis process. B-16 cells treated by Maesil extracts showed that the viability was over 80%. Maesil and maesil products in vitro and B-16 cells inhibited melanin production significantly.

• 한국식품과학회지
• /
• 제32권5호
• /
• pp.1022-1028
• /
• 2000

마이크로웨이브 추출방법과 환류냉각 추출방법을 비교한 결과, 물과 메탄올 및 에탄올 혼합용매로 추출한 경우 마이크로웨이브 추출 방법에 의하여 추출시간을 단축시키면서 환류냉각 추출방법에서와 같은 수준의 추출율과 총 폴리페놀 함량을 갖는 섬쑥부쟁이 추출물을 얻을 수 있었다. 마이크로웨이브 추출시 최적 마이크로웨이브 에너지는 $120{\sim}150$ W였고 추출시간의 경우 $4{\sim}8$분이 적당하였다. 추출에 사용한 용매들 가운데 에탄올, 메탄올보다 물 그리고 물과 에탄올 또는 메탄올 혼합용매를 사용한 추출물의 추출율, 총 폴리페놀 함량 및 전자공여 효과가 높은 것으로 나타났다.

• 한국식품영양과학회지
• /
• 제33권6호
• /
• pp.1068-1073
• /
• 2004

노루궁뎅이 버섯의 열수 추출 실험을 실시하면서 반응표면분석에 의해 추출물의 특성을 최적화하였다. 추출물의 가용성 고형분은 설정된 추출온도와 추출시간에 대해서는 영향을 받지 않는 것으로 나타났으며, 용매에 대한 시료비가 증가할수록 가용성 고형분이 증가하였으며, 페놀성 화합물과 조단백 함량도 추출온도보다는 용매에 대한 시료비가 더 큰 영향을 미치는 것으로 나타났다. 추출물의 전자공여작용은 용매에 대한 시료비와 추출온도가 증가할수록 증가하다가 최적 추출조건에서 최대점을 보이다가 이후로 감소하는 경향을 나타내었다. 이들 추출물의 특성을 모두 만족시키는 최적 추출조건은 추출온도 91.5$^{\circ}C$∼96.5$^{\circ}C$, 용매에 대한 시료비 3.5∼4.2 g/100 mL로 나타났으며 예측된 최적 추출조건의 임의의 점에서 실험한 결과, 각 반응변수들의 예측값과 실제값이 유사하였다.

• 한국컴퓨터정보학회논문지
• /
• 제26권11호
• /
• pp.157-163
• /
• 2021

본 연구에서는 운향과 식물 과실류의 식품 및 화장품에서 항산화 소재 가능성을 확인하기 위하여 6종(유자, 자몽, 레몬, 밀감, 오렌지, 탱자)을 전과와 과피로 나누어 건조 처리한 후 에탄올로 추출하여 전자공여능과 ABTS 라디탈 소거능을 측정하여 항산화 활성 효과를 비교 분석하였다. 농도 10,000 ㎍/mL에서 항산화 효과를 비교 분석한 결과, 전자공여능은 레몬의 건조 과피 추출물(88.15%)과 유자의 건조 전과 추출물(80.44%)이 높았으며, ABTS 라디칼 소거능은 레몬의 과피(94.85%)와 건조 과피(92.83%)에 높게 나타났다. 결론적으로, 6종의 운향과 과실류 중에서 레몬이 가장 높은 항산화 효과를 나타내었으며, 특히 건조된 과피 추출물에서 유의적인 효과가 있는 것으로 확인됨에 따라 식품 및 화장품에서 천연 항산화 소재로서의 활용 가능성이 있음을 확인하였다.