• 한국전기전자재료학회논문지
• /
• 제33권6호
• /
• pp.510-514
• /
• 2020

Vocational education needs to be transformed to cultivate talents with diverse fusion competencies, which is in line with the recent changes that have become a part of the complex technological developments in the 4th Industrial Revolution. Therefore, it is very important for college graduates to obtain employment skills as they are required to prepare for careers within the complex environments of future societies. With the transition to the Internet of Things (IoT)-based control in the manufacturing industry, the development of technological education and related training programs is required to cultivate practical talents for students who have acquired not only the information on existing programmable logic controller (PLC)-based technology, but also that on embedded programming technology. Therefore, to develop an NCS-based education program for analog signal processing to ensure that programming can easily be learned for cultivating practical talent, this study summarizes the opinions of field experts, selects the appropriate NCS competency unit, and designs an adequate technology education training program.

• 대한전기학회:학술대회논문집
• /
• 대한전기학회 2007년도 제38회 하계학술대회
• /
• pp.1253-1254
• /
• 2007

본 논문에서는 에너지 수요의 급증으로 세계적으로 에너지 수습의 불안이 고조되고 있는 이시점에서 차세대 친환경 에너지원으로 각광 받고 있는 연로전지에 대한 모니터링 사업에 대한 실증화 연구에 관하여 논의 하고자 한다. 한국 가스공사에서 주관한 1kw가정용 연료전지 시스템 모니터링사업은 3차년도 사업으로 나뉘어져있으며 본연구는 1차연도 사업으로 3000시간동안의 시스템의 평가 설치 운전을 하고 가정용연료전지대규모 모니터링을 통한 실측 데잉터를 분석하고 Cost down 과 시스템의 내구성향상, 부품국산화율 향상및 과제 도출을 통한 가정용 연료전지 보급기반을 목적으로 하고 있다.

• 대한설비공학회:학술대회논문집
• /
• 대한설비공학회 2006년도 하계학술발표대회 논문집
• /
• pp.80-85
• /
• 2006

The peak demand of electricity in summer season mainly comes from the day time cooling loads. Ice thermal Storage System (ITSS) uses off-peak electricity at night time to make ice for the day time cooling. In order to maximize the use of cold storage in ITSS, the estimation of day time cooling load for the building is necessary. In this study, we present a method of cooling load estimation using 5 years of normalized outdoor temperature, relative humidity, and the building construction data. We applied the hourly-based estimation to a general hospital building with relatively less sudden heat exchange and the results are compared with the measured cooling load of the building. The results show that the cooling loads estimation depends on the indoor cooling design temperature of the building.

• 대한물리의학회지
• /
• 제14권2호
• /
• pp.63-70
• /
• 2019

PURPOSE: While electrical stimulation (ES) is known to be a safe and flexible tool in rehabilitation therapy, it has had limited adoption in muscle regeneration. This study was performed to investigate whether ES can induce myogenic differentiation and to clarify the mechanism underlying the effects of ES on myogenic differentiation. METHODS: This study used rat L6 cell lines as myoblasts for myogenic differentiation. Electric stimulation was applied to the cells using a C-Pace EP culture pacer (IonOptix, Westwood, Ma, USA). The gene expressions of myogenic markers were examined using qPCR and immunochemistry. RESULTS: Our study showed that ES increased the thickness and length of myotubes during myogenic differentiation. It was found that ES increased the expression of myogenic markers, such as MyoD and Myogenin, and also activated the fusion of the myoblast cells. In addition, ES suppressed the expression of small GTPases, which can explain why ES promotes myogenic differentiation. CONCLUSION: We found that ES induced myogenic differentiation by suppressing small GTPases, inhibiting cell division. We suggest that ES-based therapies can contribute to the development of safe and efficient muscle regeneration.

• 융합보안논문지
• /
• 제18권5_1호
• /
• pp.93-98
• /
• 2018

한국수력원자력, 한국전력공사, 한국남동발전 등의 발전 제어시스템은 전력을 공급하는 국가의 주요 인프라 시설로 악의적인 해킹 공격이 진행될 경우 그 피해는 상상을 초월한다. 실제로 한국수력원자력은 해킹 공격을 당하여 내부정보가 유출되는 등 사회적인 큰 문제를 야기하였다. 본 연구에서는 최근 이슈가 되고 있는 융합보안 연구에 대해 전력회사 중심의 발전 제어시스템을 대상으로 그 환경을 분석하고, 현황을 분석하여 다양한 발전 제어시스템의 안정화를 위한 전략체계 수립과 대응책을 제시하고자 한다. 다양한 물리적 보안시스템(시설), IT 보안시스템, 출입통제시스템 등에서 나오는 데이터 형태를 정규화하고 통합하여 융합인증을 통해 전체 시스템을 통제하고, 융합관제를 통해 위험을 탐지하는 방법을 제안한다.

• 공업화학
• /
• 제33권1호
• /
• pp.71-77
• /
• 2022

바이오매스 활용을 높이기 위하여, 쌀겨 기반 활성탄소(RHAC)를 제조한 뒤 질소 플라즈마 표면처리를 수행하여 전기이중층 커패시터(EDLC) 성능을 고찰하였다. 질소 플라즈마 표면처리를 통하여, RHAC 표면에 최대 2.17%의 질소가 도입되었으며 특히, 5 min 동안 반응한 샘플의 경우 pyrrolic/pyridine계 N 작용기의 형성이 우세하였다. 또한, 실리카 제거에 의해 쌀겨 기반 탄소재에 메조기공이 형성되었고 질소 플라즈마 표면처리에 의해 탄소재 표면 거칠기가 증가하여 미세기공이 많이 형성되는 것을 확인할 수 있었다. 순환전압전류법 측정 실험으로부터, 5 mV/s의 전압 주사 속도에서 질소 플라즈마 처리된 RHAC의 비정전용량은 최대 200 F/g로, 미처리 RHAC (111 F/g)에 비교하여 80.2% 향상된 값을 나타내었다. 이러한 결과는 질소 플라즈마 표면처리로 인해 탄소재 표면에 도입된 pyrrolic/pyridine계 질소 작용기 도입과 탄소재 표면 미세기공 부피 향상으로 인한 시너지 효과인 것으로 판단된다. 본 연구는 폐기 자원을 재활용하고, 플라즈마 표면처리법을 통해 이종원소 도입을 한다는 점에서 환경적으로 긍정적인 영향을 미칠 것으로 사료된다.

• 한국가축번식학회지
• /
• 제21권3호
• /
• pp.293-302
• /
• 1997

Follicular oocytes of Grade I and II were collected from 2~6 mm ovarian follicles and matured in vitro (IVM) for 24 hrs in TCM-199 su, pp.emented with 35$\mu\textrm{g}$/ml FSH, 10$\mu\textrm{g}$/ml LH, and 1$\mu\textrm{g}$/ml estradiol-17$\beta$ at 39$^{\circ}C$ under 5% CO2 in air. They were fretilized in vitro (IVF) by epididymal spermatozoa capacitated with heparin for 12 hrs. The zygotes were then co-cultured in vitro with bovine oviducted epithelial cells (BOEC) for 7 to 9 days. The optimal time for IVM, the successful enucleation of IVM oocytes by micromanipulation at different oocyte ages after IVM, and the ideal culture system for IVM for effective IVF and in vitro development of IVM-IVF embryos was examined for in vitro production of nuclear recipient oocytes and nuclear donor embryos. To improve the efficiency of nuclear transplantation (NT) of IVF embryo into IVM follicular oocytes, this study evaluated the optimal electric condition and oocytes age for activation of IVM oocytes and in vitro development of NT embryos. In vitro development of NT embryos with preactivation or non-preactivation in enucleation oocytes, cell number of IVN-IVF embryos, and NT embryos wre also examined. The results obtained were as follows; 1. The most suitable enucleation time was at 24 hpm (83.3%) rather than that of 28 hpm(69.6%) and 32 hpm(50.0%). 2. There was no difference among the fusion rates of NT embryos at the voltages of 0.75, 1.0 and 1.5 kV/cm, but the in vitro development rates to morule and blastocyst were significantly (P<0.05) higher at the voltage of 0.75(12.5%) and 1.0kV/cm (12.6%) compared to 1.5kV/cm(0%). 3. No significant difference in activation rates were seen in NT embryos stimulated for 30, 60 and 120 $\mu$sec (71.7, 85.2 and 71.9%, respectively), but the in vitro development rates to morulae and blastocyst were significantly (P<0.05) higher in the oocytes stimulated for 30 $\mu$sec (11.6%) and 60 $\mu$sec(10.7%) than 120 $\mu$sec(0.0%). 4. The fusion rates (71.0 and 87.3%) and the in vitro development rates (9.1 and 12.7%) to morula and blastocyst were seen in the NT embryos stimulated at 28 and 32 hpm under the condition of 1.0 kV/ml, 60 $\mu$sec. However, at 24 hpm the fusion rates were 64.8% and the in vitro development to morula and blastocyst were not seen. 5. The fusion rates between the 8~12, 13~17 and 18~22-cell stage of IVM-IVF embryos were not significantly different. The in vitro development rates of the fused embryos to morula and blastocyst which were received from a blastomere of 8~12, 13~17 and 18~22-cell stages of IVM-IVF embryos were 14.9, 8.3 and 6.5%, respectively. 6. The in vitro development rate of the enucleated recipient oocytes with preactivation (24.2%) to morula and blastocyst was significantly (P<0.05) higher than that of non-preactivation (12.8%). 7. The cell numbers of NT blastocyst and IVM-IVF blastocyst cultured during 7~9 days were 63$\pm$11 and 119$\pm$23, and then their the mean cell cycle number were 5.98 and 6.89, respectively.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2012년도 제43회 하계 정기 학술대회 초록집
• /
• pp.254-255
• /
• 2012

Interest in nano-crystalline silicon (nc-Si) thin films has been growing because of their favorable processing conditions for certain electronic devices. In particular, there has been an increase in the use of nc-Si thin films in photovoltaics for large solar cell panels and in thin film transistors for large flat panel displays. One of the most important material properties for these device applications is the macroscopic charge-carrier mobility. Hydrogenated amorphous silicon (a-Si:H) or nc-Si is a basic material in thin film transistors (TFTs). However, a-Si:H based devices have low carrier mobility and bias instability due to their metastable properties. The large number of trap sites and incomplete hydrogen passivation of a-Si:H film produce limited carrier transport. The basic electrical properties, including the carrier mobility and stability, of nc-Si TFTs might be superior to those of a-Si:H thin film. However, typical nc-Si thin films tend to have mobilities similar to a-Si films, although changes in the processing conditions can enhance the mobility. In polycrystalline silicon (poly-Si) thin films, the performance of the devices is strongly influenced by the boundaries between neighboring crystalline grains. These grain boundaries limit the conductance of macroscopic regions comprised of multiple grains. In much of the work on poly-Si thin films, it was shown that the performance of TFTs was largely determined by the number and location of the grain boundaries within the channel. Hence, efforts were made to reduce the total number of grain boundaries by increasing the average grain size. However, even a small number of grain boundaries can significantly reduce the macroscopic charge carrier mobility. The nano-crystalline or polymorphous-Si development for TFT and solar cells have been employed to compensate for disadvantage inherent to a-Si and micro-crystalline silicon (${\mu}$-Si). Recently, a novel process for deposition of nano-crystralline silicon (nc-Si) thin films at room temperature was developed using neutral beam assisted chemical vapor deposition (NBaCVD) with a neutral particle beam (NPB) source, which controls the energy of incident neutral particles in the range of 1~300 eV in order to enhance the atomic activation and crystalline of thin films at room temperature. In previous our experiments, we verified favorable properties of nc-Si thin films for certain electronic devices. During the formation of the nc-Si thin films by the NBaCVD with various process conditions, NPB energy directly controlled by the reflector bias and effectively increased crystal fraction (~80%) by uniformly distributed nc grains with 3~10 nm size. The more resent work on nc-Si thin film transistors (TFT) was done. We identified the performance of nc-Si TFT active channeal layers. The dependence of the performance of nc-Si TFT on the primary process parameters is explored. Raman, FT-IR and transmission electron microscope (TEM) were used to study the microstructures and the crystalline volume fraction of nc-Si films. The electric properties were investigated on Cr/SiO2/nc-Si metal-oxide-semiconductor (MOS) capacitors.

• 한국ITS학회 논문지
• /
• 제1권1호
• /
• pp.22-32
• /
• 2002

본 연구는 기존 지점검지기와 비콘검지기 및 매설식 자동차량인식장치(Automatic Vehicle Identification : AVI)의 한계점을 극복하고자 비매설식 AVI를 개발하고 이를 통하여 도로상을 주행하는 일반적인 차량들을 프루브 차량으로 활용하여 신뢰성 있는 구간교통정보를 산출하는 방법을 개발하는 것을 목적으로 한다. 이를 위해 비매설식 자동차량인식장치를 개발하고, 국도1호선 수원$\~$평택구간(9.5km)에 설치되어 운용중인 장비에 대하여, 현장에서 수집된 자료를 분석하여 신뢰성 있는 구간교통정보 수집 가능성을 살펴보았다. 현장 실험 결과 레이저센서의 차량 검지율은 95$\%$ 이상, 차량 인식률은 87.8$\%$이며, 차량 매칭률은 약 14.3$\%$로 분석되어 도로의 괴통상황 추이를 잘 반영하는 것으로 판단되었다. 또 시스템의 신뢰도 시험 및 지속성 시험 방법에 의한 시험결과의 성능을 비친 평가하기 위하여 기존의 이와 유사한 장비를 설치하여 검수하는 각 기관의 검수기준을 적용하여 평가한 결과 본 시스템은 각 검수 기준을 모두 만족하는 것으로 나타나 현장 적용성에서 매우 뛰어난 성능을 보이고 있다 향후 연구과제로서, 설치 대상 차로 및 적정 설치구간 거리, 정보 제공 주기 등에 대한 상세한 연구 및 기존 지점 검지기 자료와의 퓨전(Fusion) 방안에 대한 연구가 필요할 컷으로 사료된다.

• Reproductive and Developmental Biology
• /
• 제28권1호
• /
• pp.13-20
• /
• 2004

This study was conducted to investigate the developmental ability of caprine embryos after somatic cell interspecies nuclear transfer. Donor cells were obtained from an ear-skin biopsy of a caprine, digested with 0.25% trypsin-EDTA in PBS, and primary fibroblast cultures were established in TCM-199 with 10% FBS. After maturation, expanded cumulus cells were removed by vigorous pipetting in the presence of 0.3% hyaluronidase. The matured oocytes were dipped in D-PBS plus 10% FBS＋7.5 $\mu\textrm{g}$/ml cytochalasin B and 0.05 M sucrose. The reconstructed oocytes were electrically fused with donor cells in 0.3 M mannitol fusion medium. After the electofusion, embryos were activated by electric stimulation. Interspecies nuclear transfer embryos with bovine cytoplasts were cultured in TCM-199 medium supplemented with 10% FBS including bovine oviduct epithelial cells for 7∼9 day. On the other hand, the NT embryos with porcine cytoplasts were cultured in NCSU-23 medium supplemented with 10% FBS for 6∼8 day at $39^{\circ}C, 5% CO_2$ in air. In caprine-bovine NT embryos, the cleavage(2-cell) rate was 36.8% in confluence and 43.8% in serum starvation. The developmental rate of morula- and blastocyst-stage embryos was 0.0% in confluence and 18.8% in serum starvation. In caprine-porcine NT embryos, the cleavage(2-cell) rate was 76.7% in confluence and 66.7% in serum starvation. The developmental rate of morula and blastocyst stage embryos was 3.3% in confluence and 3.0% in serum starvation, and no significant difference was observed in synchronization treatment between donor cells. In caprine-bovine NT embryos, the cleavage(2-cell) rate of cultured donor cells was 30.8% and 17.6% in 5∼9 and 10∼14 passage(P＜0.05). The developmental rate of morula and blastocyst stage embryos were significantly higher(P＜0.05) in 5∼9 passage(23.1%) than in 10∼14 passage(0.0%) of cultured donor cells. In caprine-porcine NT embryos, the cleavage rate was significantly higher(P＜0.05) in 5∼9 passage(86.7%) than in 10∼14 passage(50.0%) of cultured donor cells. The developmental rate of morula and blastocyst stage embryos were 3.3 and 0.0% in 5∼9 and 10∼14와 passage of cultured donor cells. In caprine-bovine NT embryos, the developmental rate of morula and blastocyst stage embryos were 22.6% in interspecies nuclear transfer, 33.9% in in vitro fertilization and 28.1% in parthenotes, which was no significant differed. The developmental rate of morula and blastocyst stage embryos with caprine-porcine NT embryos were lower(P＜0.05) in interspecies nuclear transfer(5.1%) than in vitro fertiltzation(26.9%) and parthenotes(37.4%).