• 생약학회지
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• 제44권2호
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• pp.182-187
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• 2013

This study was conducted to investigate glutathione S-transferase(GST) activity, tyrosinase inhibitory effect and elastase inhibitory effect in persimmon calyx(calyx of Diospyros kaki Thunberg) for screening of functional materials from natural products. As a result, EtOAc extract of persimmon calyx turned out to be having tyrosinase inhibitory effect and elastase inhibitory effect. The active constituents of tyrosinase and elastase inhibitory effect were isolated from EtOAc extract of persimmon calyx. Their structure of compounds were identified as ursolic acid and (-)-daucosterol by spectroscopic evidence, respectively.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1996년도 춘계학술대회
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• pp.165-165
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• 1996

균의 감염에 의해 유도되는 패혈성 shock는 균이 분비하는 elastase가 관여하며, 외부에서 serine pretense inhibitor의 biopolymer의 투여로 균에 의해 유도된 패혈성 shock를 억제시킬 수 있다고 보고되고 있다. 이에 본 연구진은 패혈성 치료제의 개발의 목적으로, 국내에서 민간 약으로 많이 이용되고 있는 백편두로부터 새로운 elastase inhibitor를 분리, 정제하여 부분 아미노산 서열 및 특성을 조사하였기에 발표하고자 한다. 백편두 추출액을 여러 차례에 걸쳐 column chromatography을 수행하면서 얻어지는 각 fraction에 대하여 elastase MCA-기질 및 trypsin MCA-기질을 이용하여 활성 측정 후 elastase 기질 및 trypsin 기질에 대하여 활성을 억제하는 fraction들을 모아 $C_{18}$ open column chromatography 및 $C_{18}$ HPLC 과정을 수행하여 2종류의 trypsin 활성 억제 물질과 1종류의 elastase inhibitor를 분리, 정제하여 각각을 Ti1, Ti2 및 Ti3로 명명하였다. 전기영 동 상에서 단일 hand를 확인한 후 각각의 inhibitor들의 부분 아미노산 서열을 결정하였다.

• 한국식품영양학회지
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• 제21권2호
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• pp.143-147
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• 2008

To develop a new anti-wrinkle agent from medicinal plants, this study investigated the optimal conditions for extracting elastase inhibitor from Rheum undulatum L. Maximal extraction occurred by using 70% methanol at $50^{\circ}C$ for 24 hr; the elastase inhibitory activity was 60.4%($IC_{50}:6.7{\times}10^3{\mu}g/m{\ell}$). Systematic solvent extraction, thin layer chromatography, silica gel column chromatography, Sephadex LH-20 column chromatography, and reverse-phase high performance liquid chromatography were used in the partial purification of the elastase inhibitor. The compound was soluble in dimethylsulfoxide, methanol, and ethanol, and had maximum absorption spectra at 231.5 nm and 275.5 nm.

• 약학회지
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• 제48권1호
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• pp.82-87
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• 2004

Mungbean trypsin inhibitor (MBTI) was isolated and purified from Mung bean which has been used as a galenic and traditional food. MBTI and poly(ethylene glycol) were conjugated by using water soluble carbodiimide. We evaluated the therapeutic value of the MBTI and MBTI-PEG conjugate using animal models, sublethal septic shock model in guinea pig caused by pseudomonal elastase, shock model in rat caused by lipopolysaccharide, and the vascular permeability test by using pseudomonal elastase. In two shock model in guinea p Is and in rat, hypotesion shock was inhibited by pretreatment of MBTI. And also the vascular permeability caused by pseudomonal elastase reduced by pretreatment of MBTI. Also, therapeutic value of the MBTI-PEG conjugate was evaluated by using the sublethal septic shock model caused by pseudomonal elastase. The MBTI-PEG conjugate was more effective than native MBTI against pseudomonal elastase induced septic shock in guinea pig model.

• 동의생리병리학회지
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• 제25권2호
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• pp.306-310
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• 2011

Elastic fibers are found in the skin, lungs, arteries, veins and other structures. Elastases destroy the elastic fibers and cause the emphysema and pulmonary hypertension. Oxidative stress is needed for these pathologic changes. Accordingly, present study was designed to investigate the effect of Albizziae Cortex extracts (ACE) on elastase activity and anti-oxidative effects of ACE. The in vitro inhibitory effects on elastase and di(phenyl)-(2,4,6-trinitrophenyl)iminoazanium (DPPH) and nitric oxide (NO) free radical scavenging activities of ACE were measured. The elastase activity was significantly inhibited by ACE. DPPH and NO free radicals were significantly scavenged as well. ACE showed the elastase-inhibiting effects and anti-oxidative activities in vitro. These results suggest that ACE may have potential roles in the treatment of pulmonary emphysema and pulmonary hypertension.

• 대한화장품학회지
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• 제26권1호
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• pp.163-186
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• 2000

Elastase (EC 3. 4. 4. 7)는 구조 단백질인 fibronectin, collagen 그리고 elastin을 포함한 다양한 단백질들을 분해한다. Elastase는 생체 염증 방어의 중요한 성분이며, $\alpha$$_1$-proteinase inhibitor ($\alpha$$_1$PI)와 같은 생체 내부에 있는 단백질 분해 효소 저해제들에 의해 조절된다. 그러나, 만성 염증의 결과, elastase와 $\alpha$$_1$PI 사이의 균형이 elastase 쪽으로 기울어지면 조직 파괴가 일어나게 된다. 본 연구자들은 이미 피부의 진피내에서 백혈구로부터 과다하게 분비되는 elastase를 저해하는 물질을 찾기 위하여, 150 여가지의 한방식물 추출물에 대해 elastase 저해활성을 스크리닝 (screening) 하여, 빈랑 (Areca catecheu) 추출물이 가장 높은 저해활성을 보여줌을 확인하였다. 본 연구에서는 유효성분을 찾기 위하여 빈랑을 90% 에탄올로 추출한 다음, 다양한 용매를 사용하여 분획하였고, 각 분획에 대해 elastase 저해활성을 측정하였다. 이 중 저해 활성이 뛰어난 분획은 silica gel 컬럼 크로마토그래피, Preparative TLC 그리고 역상 HPLC를 실시하였다. 역상 HPLC에서 elastase 저해 활성이 있는 peak은 성분을 확인할 수 있는 여러 가지 정색 반응과 UV, IR 스펙트럼을 통하여 phenol 성 물질임을 확인하였다. 빈랑으로부터 정제한 phenol 성 물질은 돼지 췌장 elastase (Porcine pancreatic elastase : PPE)와 사람 백혈구 elastase (Human neutrophil elastase ; HNE)에 대한 $IC_{50}$/ 값이 각각 26.9 $\mu\textrm{g}$/$m\ell$과 60.8 $\mu\textrm{g}$/$m\ell$로서, 기존에 elastase의 저해제로 알려진 oleanolic acid (각각 76.5 $\mu\textrm{g}$/$m\ell$, 219.2 $\mu\textrm{g}$/$m\ell$)와 ursolic acid (각각 31.0 $\mu\textrm{g}$/$m\ell$, 118.6 $\mu\textrm{g}$/$m\ell$)에 비하여 더 우수한 저해 활성을 나타내었으며, PPE와 HNE에 대하여 기질과 경쟁적으로 반응함을 알 수 있었다. 또한 빈랑으로부터 정제한 phenol 성 물질이 염증 과정 중에 발생하는 free radical을 소거할 수 있는지를 조사한 결과, free radical을 50% 소거시키는 농도값이 6 $\mu\textrm{g}$/$m\ell$로 표준물질인 비타민 C (19 $\mu\textrm{g}$/$m\ell$)나 butylated hydroxyl toluene (18.5 $\mu\textrm{g}$/$m\ell$)보다 우수한 효과를 나타내었다. 그리고 mast cell내에서 활성화되며 hyaluronic acid를 분해하는 hyaluronidase에 대한 저해를 조사한 결과, $IC_{50}$/ 값이 210 $\mu\textrm{g}$/$m\ell$로서 효과적인 저해 활성을 나타내었다 따라서, 본 연구에서 빈랑으로부터 분리한 phenol 성 물질은 피부 결합 조직을 구성하는 단백질들을 보호함으로써 피부 노화를 억제하는 효과를 나타낼 것으로 생각된다.

• Journal of Acupuncture Research
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• 제28권6호
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• pp.35-42
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• 2011

목적 : Elastin 섬유는 피부, 폐, 동맥, 정맥 등 조직에서 발견된다. Elastin 섬유의 손실은 대동맥, 동맥, 세동맥의 탄력 등과 연관되어 폐기종, 혈액순환 부전 및 폐동맥 고혈압을 악화시킬 수 있다. 본 연구는 당귀약침액의 elastase 효소활성에 대한 효과와 항산화 효과에 대하여 연구하고자 계획되었다. 방법 : 당귀약침액의 elastase 억제효과와 DPPH, 및 NO 소거능을 측정하여 항산화 효과를 측정하였다. 결과 : 당귀약침액에서 통계적으로 유의한 elastase 활성 억제효과를 관찰할 수 있었다. 또한 통계적으로 강한 DPPH free radical 소거 효과가 관찰되었다. 그리고 NO 소거효과를 관찰할 수 있었으며, 그 효능은 pH 6.0에서 가장 강한 효능을 보였다. 결론 : 본 연구 결과에 의하면, 당귀약침액에는 유효한 elastase 활성 억제효과와 강한 항산화 효과가 있어, 폐기종 및 폐동맥 고혈압 치료에 유효한 작용이 있을 것으로 사료된다.

• Journal of Microbiology and Biotechnology
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• 제13권6호
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• pp.1021-1026
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• 2003

Numerous secreted and cell-associated virulence factors have been proposed to account for the fulminating and destructive nature of Vibrio vulnificus infections. Among the putative virulence factors are an elastase, elastolytic protease, and a cytolytic hemolysin. Effects of the elastase on the hemolysin were assessed by evaluating changes of hemolytic activities either in the presence or absence of the protease. Although hemolytic activity in the culture supernatant was lowered by the purified elastase added in vitro, the cellular level of hemolytic activity was unaffected by the mutation of vvpE encoding the elastase. Growth kinetic studies revealed that hemolysin reached its maximum level in the exponential phase of growth, and the elastase appeared at the onset of the stationary phase. These results have provided insight into the regulation of virulence factors: temporally coordinate regulation of virulence factors is essential for the overall success of the pathogen during pathogenesis.

• 대한한방내과학회지
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• 제31권1호
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• pp.84-101
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• 2010

Objectives : This study aimed to evaluate the protective effects of Seonpyejeongcheon-tang (SJT) on elastase-induced lung injury. Materials and Methods : The extract of SJT was treated to A549 cells and an elastase-induced lung injury mouse model. Then, various parameters such as cell-based cytoprotective activity and histopathological findings were analyzed. Results : SJT showed a protective effect on elastase-induced cytotoxicity in A549 cells. This effect was correlated with analysis for caspase 3 levels, collagen and elastin contents, protein level of cyclin B 1, Cdk1, and Erk1/2, and gene expression of TNF-$\alpha$ and IL-$1{\beta}$ in A549 cells. SJT treatment also revealed a protective effect on elastase-induced lung injury in mouse model. This effect was evidenced via histopathological findings, including immunofluoresence stains against elastin, collagen, and caspase 3, and protein levels of cyclin B1, Cdc2, and Erk1/2 in lung tissue. Conclusion : These data suggest that SJT has pharmaceutical properties on lung injury. This study thus provides scientific evidence for the efficacy of SJT for clinical application to patients with chronic obstructive pulmonary disease.

• 대한약침학회지
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• 제14권1호
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• pp.61-69
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• 2011

Objectives : Elastic fibers are found in the skin, lungs, arteries, veins and other structures. Elastases destroy the elastic fibers and cause the emphysema and pulmonary hypertension. Oxidative stress is needed for these pathologic changes. Accordingly, present study was designed to investigate the effect of Yukmigeehwang-hwan extracts (YHE) on elastase activity and anti-oxidative effects of YHE. Methods : The inhibitory effects on elastase and DPPH and NO free radical scavenging activities of YHE were measured. Results : The elastase activity was significantly inhibited by YHE. YHE significantly scavenged DPPH and NO free radicals as well. Conclusion : YHE showed the elastase-inhibiting effects and anti-oxidative activities in vitro. These results suggest that YHE may have potential roles in the treatment of pulmonary emphysema and pulmonary hypertension.