• Title/Summary/Keyword: egt

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Characterization of Ecdysteroid UDP-Glucosyltransferase Gene Promoter from Bombyx mori Nucleopolyhedrovirus

  • Zhang, Zhi-Fang;Shen, Xing-Jia;Yi, Yong-Zhu;Tang, Shun-Ming;Li, Yi-Ren;He, Jia-Lu;Wu, Xiang-Fu
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.2
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    • pp.169-174
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    • 2004
  • Bombyx mori nucleopolyhedrovirus(BmNPV) ecdysteroid UDP-glucosyltransferase gene (egt) promoter fragments of different lengths were amplified from BmNPV ZJ-8 genomic DNA by PCR. Reporter plasmids pBmegt542-luc, pBmegt309-luc and pBmegtl59-luc with luciferase (lue) driven by egt promoters were constructed. Both in vitro and in vivo expressions showed that BmNPV egt promoter activity requires the transactivation of viral factor(s), and expression of luc was detected earliest at 24 hrs post infection (pi). BmNPV ZJ-8 homologous region 3 (hr3) increased the expression of luc by over 1,600-fold. Molting hormone of 1.0 - 2.0 $\mu\textrm{g}$/$m\ell$ can dramatically down regulate expression of luc. Juvenile hormone analogue of 0.5-2.0 ${\mu}g$/$m\ell$ increased expression of luc by 145.8% to 75.7%. Deletion assay revealed that the promoter fragment of 159 bp contains the basal promoter structure; Promoter fragments of 309 bp and 542 bp showed similar but much higher transcriptional activities than that of 159 bp, suggesting that nucleotide from -159 to -309 nt upstream the translation initiation site harbors the main cis-acting elements.

Ecdysteroid Stimulates Virus Transmission in Larvae Infected with Bombyx mori Nucleopolyhedrovirus

  • Kang, Kyung-Don;Lee, Eun-Jung;Kamita, Shizuo George;Maeda, Susumu;Seong, Su-Il
    • BMB Reports
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    • v.33 no.1
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    • pp.63-68
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    • 2000
  • Most baculoviruses have an ecdysteroid UDP-glucosyltransferase (egt) gene, whose product inactivates ecdysteroid within the infected host. Bomhyx mori larvae infected with BmEGTZ, a mutant B. mori nucleopolyhedrovirus (BmNPV) in which the egt gene has been inactivated, die more rapidly compared to larvae infected with wild-type BmNPV. In this study, the profile of hemolymph proteins, and progression of virus infection in BmEGTZ- and BmNPV-infected B. mori larvae, was analyzed by SDS-PAGE and histochemically. These analyses showed that virus-encoded and virus-induced proteins were expressed quicker in BmEGTZ-infected larvae than in BmNPV-infected larvae. This suggests that the decrease in time to death, following BmEGTZ infection, results from the stimulation of virus-specific protein expression. In order to examine the effect of ecdysteroid on virus transmission, the profile of hemolymph proteins, and progression of virus infection, were analyzed following an ecdysteroid injection of BmEGTZ- or BmNPV-infected larvae. In the BmNPV-infected larvae, ecdysteroid treatment had no apparent effect on hemolymph protein expression. This suggests that the injected ecdysteroid was inactivated by the BmNPV-expressed ecdysteroid UDP-glucosyltransferase. An Ecdysteroid injection into BmEGTZ-infected larvae increased the speed of virus-specific protein expression and virus transmission. These results suggest that ecdysteroid stimulates protein expression, which in tum results in the stimulation of virus transmission.

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Evolutionary game theory-based power control for uplink NOMA

  • Riaz, Sidra;Kim, Jihwan;Park, Unsang
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.12 no.6
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    • pp.2697-2710
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    • 2018
  • Owing to the development of Internet of Things (IoT), the fifth-generation (5G) wireless communication is going to foresee a substantial increase of mobile traffic demand. Energy efficiency and spectral efficiency are the challenges in a 5G network. Non-orthogonal multiple access (NOMA) is a promising technique to increase the system efficiency by adaptive power control (PC) in a 5G network. This paper proposes an efficient PC scheme based on evolutionary game theory (EGT) model for uplink power-domain NOMA system. The proposed PC scheme allows users to adaptively adjusts their transmit power level in order to improve their payoffs or throughput which results in an increase of the system efficiency. In order to separate the user signals, a successive interference cancellation (SIC) receiver installed at the base station (BS) site. The simulation results demonstrate that the proposed EGT-based PC scheme outperforms the traditional game theory-based PC schemes and orthogonal multiple access (OMA) in terms of energy efficiency and spectral efficiency.

New Ignition Method and Ignition Recognition Logic for a Microturbine (마이크로터빈의 새로운 점화 기법과 점화 인식 로직 개발)

  • Kim, Gi-Rae;Choi, Young-Kyu;Rho, Min-Sik
    • Journal of Institute of Control, Robotics and Systems
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    • v.13 no.2
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    • pp.179-186
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    • 2007
  • This paper presents new ignition method and ignition recognition logic for a microturbine. New ignition method is designed by constant speed control of a microturbine with pre-determined time during a ignition period. It make more accurate air-fuel ratio as well as give enough time to ignition system to have full performance under cold temperature. And ignition recognition logic is designed by observing output current change of inverter by generating output torque of a microturbine in the instant of ignition. For filtering a output torque current of inverter with high frequency, we applied a moving average method. So far, ignition recognition is usually implemented by measuring of exhausted gas temperature(EGT) of microturbine. The proposed logic can give more accurate judgement of ignition as well as keep a good working of starting system under out of order a temperature measuring system and biased initial value of EGT sensor. Finally, the two proposed logics are proved by field operating a microturbine under various conditions.

Performance Analysis of Precoded LTE-Advanced Uplink System (LTE-Advanced 시스템의 선부호화된 상향 링크 성능 분석)

  • Kim, Sang-Gu;Li, Xun;Kim, Young-Ju
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.48 no.5
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    • pp.8-15
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    • 2011
  • LTE-Advanced aims at peak data rates of 1Gbits/s for the downlink and 500 Mbits/s for the uplink, which can be accomplished only by using wide spectrum allocation of 100MHz as well as advanced multiple input multiple output antenna techniques to the uplink. This paper analyzes the uplink precoding techniques which include LTE codebook of downlink, singular value decomposition codebook, and equal gain transmission codebook over LTE defined single carrier frequency division multiplexing systems. Finally considering nonlinear transmit power amplifier model, it is shown that link-level performance of EGT is superior to those of any other precoding schemes.

A Fast Index Search Algorithm for Codebook-based Equal Gain Transmission Beamforming System (코드북을 이용한 동 이득 전송 빔포밍 시스템에서 빠른 인덱스 탐색 기법)

  • Park, Noe-Yoon;Kim, Young-Ju;Li, Xun;Lee, Kwan-Seob
    • Journal of the Institute of Electronics Engineers of Korea TC
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    • v.45 no.12
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    • pp.83-89
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    • 2008
  • Simple but effective fast codebook searching algorithms for quantized EGT(equal gain transmission) are presented. Using newly defined metric, rough searching selects a few candidate weight vectors. Using the conventional metric, accurate searching determines the optimal vector among the candidates. This strategy reduces the entire searching time. When the number of transmit antenna is two or three, the searching time reduces more than a half less than the conventional searching time. Moreover, the more the size of codebook increases, the more the searching time reduction also increases.

EROGOTHIONEINE RESCUES PCl2 CELLS FROM BETA-AMYLOID-INDUCED APOPTOTIC DEATH

  • Jang, Jung-Hee;Surh, Young-Joon
    • Proceedings of the PSK Conference
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    • 2003.10b
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    • pp.141.2-142
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    • 2003
  • beta-Amyloid (A$\beta$) peptide is the major component of senile plaques and considered to have a causal role in the development and progression of Alzheimer's disease. There has been compelling evidence supporting that $A\beta$-induced cytotoxicity is mediated through oxidative and/or nitrosative stress. Recently, considerable attention has been focused on dietary manipulation of oxidative and/or nitrosative damage. L-Egrothioneine (EGT) is a low-molecular weight naturally occurring thiol compound of dietary origin which exists in milimolar concentrations in the brain, liver, kindney, erythrocytes, ocular tissues and in seminal fluids of mammals. (omitted)

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Development of a Small Jet Engine Performance Test Device by Applying the Real-time Gas Turbine Engine Simulator (실시간 가스터빈 엔진 시뮬레이터를 적용한 소형 제트엔진 성능시험장치 개발)

  • Kho, Seonghee;Kong, Changduk;Ki, Jayoung
    • Journal of the Korean Society of Propulsion Engineers
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    • v.18 no.6
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    • pp.42-49
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    • 2014
  • Test device using virtual engine simulator can help reduce the number of engine tests through tests similar to the actual engine tests and repeat the test under the same condition, and thus reduce the engine maintenance and operating costs. Also, as it is possible to easily implement extreme conditions in which it is hard to conduct actual tests, it can prevent engine damages that may happen during the actual engine test under such conditions. In this study, an upgraded small jet engine performance test device was developed that can conduct both real and virtual engine test by applying real-time engine model to the existing micro jet engine performance test device that was previously developed by authors. This newly developed multi-purpose small jet engine performance test device is expected to be used for various educational and research purposes.

A Development of EMAS (Easy Maintenance Assistance Solution) for Industrial Gas Turbine (산업용 가스터빈을 위한 정비지원 시스템 개발에 관한 연구)

  • Kang, Myoungcheol;Ki, Jayoung
    • Journal of the Korean Society of Propulsion Engineers
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    • v.21 no.3
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    • pp.91-100
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    • 2017
  • The solution was developed for the maintenance decision support of combined cycle power plant gas turbine. The developed solution was applied to MHI501G gas turbine and is, in present, on the process of field test at GUNSAN combined cycle power plant, South Korea. The developed solution provides the calculated result of optimal overhaul maintenance period through following modules: Real Time Performance Monitoring, Model-Based Diagnostics, Performance Trend Analysis, Optimal Overhaul Maintenance Interval, Compressor Washing Period Management, and Blade Path Temperature Analysis. Model-Based Diagnostics module analyzed the differences between the data of gas turbine performance model and the online measurement. Compressor washing management module suggests the optimal point of balancing between the compressor performance and the maintenance cost.