• 농업과학연구
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• 제47권1호
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• pp.11-18
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• 2020

Eggs are likely to be used in agriculture because they can provide enough nutrients for crop growth. Statistics show that a large number of eggs are lost due to breakage before reaching the final consumer. The purpose of this study was to make a natural liquid fertilizer as a substitute for chemical fertilizers using broken eggs as a resource and to evaluate the efficiency of the formulated fertilizer. To make the liquid fertilizer, the broken eggs and distilled water were mixed at ratios of 6 : 4 and 4 : 6. Then, effective micro-organisms (EM) and sugar were added, and the mixture was fermented. The temperature and electrical conductivity (EC) increased gradually with the fermentation while the pH decreased. When evaluated following the seed germination index method of the compost, it was found that the fertilizer matured 10 days after the beginning of the experiment. The growth experiment was conducted with lettuce in which the fermented liquid fertilizer was compared with a commercial liquid fertilizer. The 6 : 4 treatment produced plants with the densest fresh shoot and roots weighing 41.6 and 4.6 g, respectively. The number of leaves (12.3 per plant) was also the highest for the 6 : 4 treatment. Soil analysis showed that the soil pH was improved, and the soil organic matter was increased in the fermented liquid fertilizer treatment.

• 한국패류학회지
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• 제31권2호
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• pp.113-121
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• 2015

Gametogenesis, mating behaviour and spawning of Octopus ocellatus were investigated by histological study. This species is dioecious, and showed a protandry phenomenon. Ooogenesis (in females) and spermatogenesis (in males) can be classified into 3 stages, respectively. O. ocellatus copulates in one of two ways: a male may leap upon a female, mounting her mantle, or a male may sit near the female and extend the hectocotylized third right arm toward her. Spawning occurred between April and June in females, and between March and May in males of O. ocellatus. The spawning period was once a year and the peak took place between May and June. A number of flatened follicle cells, which were attached to an oocyte, were involved in vitellogenesis in the cytoplasm of the vitellogenic oocyte (maturing oocyte), and formation of chorion membrane (secondary egg membrane) of the ovarian eggs. Fecundity per female closely related to GSI was 294-660 eggs (average, 429 eggs). The diameters of the ovarian eggs surrounded by chorion membrane were approximately in the range of 10.10-2.50 mm. Each ovarian egg laid by a female was connected to an egg string. Each egg string was 1-5.5 cm (average 3.6 cm). The total number of eggs laid by a female of this species ranged 218-314, the egg sizes were independent to the size of female adult. this species has a life mode showing some special reproductive characteristics of an annual semelparity as shown in Octopodidae species because we have never seen a female spawning a second time.

• Entomological Research
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• 제48권6호
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• pp.550-558
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• 2018

To develop a system for the mass production of Protaetia brevitarsislarvae (Pbl), this study analyzed the oviposition characteristics of third instar Pbl through successive rearing and cold treatments. In the first generation, females laid 50 eggs each on average, and spawned higher numbers of eggs for the first 5 days but then fewer after this. However, all of the $2^{nd}$-generation females that were produced through the successive rearing of eggs from the $1^{st}$ generation did not spawn. When test insects (TI) were raised at $32^{\circ}C$, their $mean{\pm}SE$ pupal period and emergence rate were $28.4{\pm}1.9$ days and 87%, respectively. However, most individuals raised in cold treatments ($5^{\circ}C$ and $-5^{\circ}C$) without a pre-cooling period did not survive. Individuals exposed to a pre-cooling period, however, had a 7-10 days longer pupal period than those raised at $32^{\circ}C$, and their emergence rate (90%) was slightly higher. Different cold treatment temperatures ($0^{\circ}C$, $3^{\circ}C$, or $5^{\circ}C$) and periods (30 or 60 days) were tested in further experiments. The experimental group kept for 30 days at $0^{\circ}C$ and $3^{\circ}C$ had the shortest pupal period of all, and emergence rates exceeded 90% in most groups. With respect to the number of eggs spawned by the second generation females after cold treatments, the experimental groups held in cold treatments at $5^{\circ}C$ for 30 and 60 days were found to spawn the most eggs. Consequently, the experimental group kept at $5^{\circ}C$ for 30 days was the most effective set of conditions tested during the entire breeding period.

• Parasites, Hosts and Diseases
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• 제23권2호
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• pp.253-259
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• 1985

요충 자충의 길이별 보유충란수가 어떤 양상을 나타내는자를 관찰하였다. 충란수를 관찰한 요충은 Cho et al. (1981)이 경기도 안양시 보육원 원아에게 구충제를 투여한 후 배출된 충체를 고정하여 두었던 것중203 마리였다. 해부현미경하에서 완전하다고 판단한 충체를 골라, 길이를 측정하고, 0.1N Na OH 용액으로 충체를 연화시켰으며, 측정판에서 충체를 해부침으로 파괴한 다음 난각을 형성한 충란의 숫자를 세었다. 그 결과를 요약하면 다음과 같다. 1. 난각을 형성한 충란은 요충 자층의 길이 5.50mm일 때부터 나타나기 시작하였다. 2. 충란을 보유한 충체의 비율은 충체 길이 5.50~5.99mm에서 25%, 600~6.49mm에서 53.3%, 6.50~6.99mm에서 86.7%, 7.00~7.49mm에서 95.2%이었으며 7.50mm이상 충체에서는 100%가 충란을 보유하였다. 3. 충체 길이별 보유 충란수의 평균과 표준편차는 5.49mm이하에서 0, 5.50~5.99mm에서 $19{\pm}50$개, 6.00~6.49mm에서 $734{\pm}$1,597개, 6.50~6.99mm에서 $1,473{\pm}1,606$개, 7.00~7.49mm에서 $1,530{\pm}2,055$개, 7.50~7.99mm에서 $2,567{\pm}2,046$개, 8.00~8.49mm에서 $5,598{\pm}2,470$개, 7.50~8.99mm에서 $9,318{\pm}2,651$개, 9.00~9.49mm에서 $10,678{\pm}2,892$개, 9.50~9.90mm에서 $13,323{\pm}1,778$개 이었다. 4. 충체 길이별 충란 보유수는 충체 개개에 따라 특히 5.5~8.0mm의 범위에서 상대적으로 큰 변이폭을 나타냈으나 9.0mm이상이 되면 보유 충란수는 대부분 충체에서 10,000개 이상 16,600개의 범위에 있으면서 변이폭도 좁아지고 있다. 이상의 결과에서 요충 자충의 충란 생산은 감염후 28~32일에 시작하며 생산 초기단계 충란 보유수는 충체에 따라 차이가 심하다가 9.0mm이상으로 자라면서 거의 모든 충체가 충란을 10,000~16,000개 보유하면서 충란을 가득 갖고 항문으로 이행한다고 생각하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제11권2호
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• pp.107-111
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• 2005

Impact of microsporidian infection and season on reproductive potential of Bombyx mori L. was investigated in the laboratory. Microsporidian infection significantly (P<0.01) reduced fecundity and hatching and increased sterility and mortality of eggs. Among the microsporidia, Nosema sp. 2 infected silkworm produced eggs with least fecundity and hatching percent as well as highest dead and sterile eggs followed by Nosema sp. 1 and N. bombycis. Microsporidia, in general, significantly reduced fecundity and hatching percent of eggs and increased number of dead and sterile eggs in all the three seasons except N. bombycis in July - August (S3) and Nosema sp. 2 in January February (S1). Since, seed production is the anchor sheet of mulberry sericulture, coefficient of egg lying is considered as an important aspect and the industry quite often facing shortage of disease free layings. The present study indicates that B. mori is more susceptible to microsporidia during S2 followed by S3 and S1 and Nosema sp2. is most virulent followed by Nosema spl. and N. bombycis.

• Asian Pacific Journal of Cancer Prevention
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• 제17권1호
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• pp.281-283
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• 2016

The Cholangiocarcinoma is a. The risk of development of cholangiocarcinoma, generally a rare type of a liver tumor, increases during infection of Opisthorchiasis. For this reason the timely detection of Opisthorchiasis is important for Cholangiocarcinoma prevention. There are many studies which concern the detection of pathogenesis of Opisthorchis viverrini infection but a little known about Opisthorchis felineus. In this study we investigate a correlation of the eggs which are found in a faeces and are comparable with a serum Ig G and Ig M antibody level that were detected with ELISA test in a large group of patients. The result is showing positive correlation between evidence of the Opisthorchis felineus eggs that were found in a faeces and antibody Ig G and Ig M level in a serum. Moreover the combination of two methods can improve the Opisthorchiasis diagnostic: the serum antibody and faeces investigation of eggs.

• 한국임상수의학회지
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• 제4권2호
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• pp.491-498
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• 1987

In an effort to compare parasite egg detection efficiencies, fecal samples from 231 dairy and Korean native cattle in the area of Hongsung - Gun, Kangwon-Do, were examined by the sedimentation and floatation methods. The results obtained were summarized as follows : 1. Detection rates of Fasciola hepatica were 27.2% by the sedimentation method and 61.6% by the floatation method, and those of Paramphistomum spp., were 48.9% and 66.2% respectively. 2. In the Fasciola hepatica, the numbers of cattle contained over a hundred eggs were one by the sedimentation method, but ten by the floatation method. For the Paramphistomum spp., the numbers of cattle contained over a hundred eggs were six by the sedimentation method, but thirty three by the floatation method. Thus, the number of the parasite eggs detected were greater when examined by the floatation method than by the sedimentation method. 3. The numbers of cattle from which eggs were detected only by the sedimentation methods were three and four for Fasciola hepatica and Paramphistomum spp., respectively, while those by the floatation method only were 55 and 44. respectively. 4. Trematodes, nematodes. protozoa and cestodes were floatated by the floatation method using zinc sulfate solution (specific gravity 1.27).

• Clinical and Experimental Reproductive Medicine
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• 제27권3호
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• pp.253-259
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• 2000

Objective: To verify the effect of Matrigel, a ECM complex from Engelbreth-Holm-Swarm (EHS) mouse sarcoma on the preimplantation development and apoptosis of mouse fertilized eggs. Method: Late pronucleus stage eggs were cultured through the blastocyst stage in the presence of Matrigel (0.5%, v/v). Characteristics of apoptosis and cell number assesed by Hoecst staining and TUNEL labeling at the blastocyst stage, respectively. Results: Morphological development, number of cells per embryo was significantly increased but rate and number of TUNEL positive nuclei of the embryo were decreased in the presence of Matrigel. Conclusion: This result suggested that at low concentration of Matrigel improves both viability and morphological development in the preimplantation mouse embryos.

• Journal of Ecology and Environment
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• 제36권1호
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• pp.11-17
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• 2013

The life cycle of butterflies is closely related to the growth of food plants and, through a prolonged coevolutionary process, has undergone an ecological adaptation. So, it is important that control the egg-laying number and place to secure enough food plant by female adult to guarantee the survival of the larvae. To study whether oviposition control of the Luehdorfia puziloi takes into greater consideration food plant leaf biomass or leaf abundance, correlation among the egg cluster size, the leaf size, and the number of leaves around egg clusters was investigated. According to the results, the egg clusters size exhibited positive correlation with the leaf size of food plants on which eggs had been laid but did not do so with the number of surrounding leaves. In addition, the number of egg clusters laid exhibited positive correlation with the number of surrounding food plant leaves but not with the leaf size on which eggs had been laid. Consequently, for the Luehdorfia puziloi, the females' recognition of the leaf size seems to be the most important factor in the egg cluster size, and the number of egg clusters had positive correlation with food plant density per unit area.

• Parasites, Hosts and Diseases
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• 제54권1호
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• pp.47-53
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• 2016

Echinostomes are intestinal trematodes that infect a wide range of vertebrate hosts, including humans, in their adult stage and also parasitize numerous invertebrate and cold-blooded vertebrate hosts in their larval stages. The purpose of this study was to compare Echinostoma malayanum parasite growth, including worm recovery, body size of adult worms, eggs per worm, eggs per gram of feces, and pathological changes in the small intestine of experimental animals. In this study, 6-8-week-old male hamsters, rats, mice, and gerbils were infected with echinostome metacercariae and then sacrificed at day 60 post-infection. The small intestine and feces of each infected animal were collected and then processed for analysis. The results showed that worm recovery, eggs per worm, and eggs per gram of feces from all infected hamsters were higher compared with infected rats and mice. However, in infected gerbils, no parasites were observed in the small intestine, and there were no parasite eggs in the feces. The volume of eggs per gram of feces and eggs per worm were related to parasite size. The results of histopathological changes in the small intestine of infected groups showed abnormal villi and goblet cells, as evidenced by short villi and an increase in the number and size of goblet cells compared with the normal control group.