• Asian-Australasian Journal of Animal Sciences
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• v.32 no.9
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• pp.1407-1413
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• 2019

Objective: This experiment aimed to investigate the effect of different sources and inclusion levels of dietary fat on productive performance and egg quality in laying hens raised under hot environmental conditions. Methods: A total of 480 Hy-Line Brown laying hens at 31 wk of age were randomly allotted to 1 of 5 experimental diets. The control diet contained 2,800 kcal/kg nitrogen-corrected apparent metabolizable energy with no fat addition. Four additional diets were prepared by adding 2.0% or 4.0% of animal fat (AF) or soybean oil (SO). Energy and nutrient concentrations were consistent among all diets. Diets were fed to hens for 4 weeks. Average daily room temperature and humidity were $26.7^{\circ}C{\pm}1.52^{\circ}C$ and $77.4%{\pm}4.50%$. The heat stress index was approximately 76, indicating that hens were raised under heat stress conditions. Results: Final body weight (BW) was greater (p<0.05) for hens fed diets containing 2.0% or 4.0% AF than for those fed the control diet or diets containing 2.0% or 4.0% SO. The BW gain and feed intake were greater (p<0.05) for hens fed diets containing additional AF or SO than those fed the control diet. Eggshell thickness was the greatest (p<0.05) for hens fed the control diet, but the least (p<0.05) for hens fed diets containing 4.0% SO. Egg yolk color was the greatest (p<0.05) for hens fed the control diet, but the least (p<0.05) for hens fed diets containing 4.0% SO. Conclusion: Inclusion of supplemental fat (AF and SO) in diets exhibits preventative effects on BW loss for hens raised under hot environmental conditions when energy and nutrient concentrations in diets were maintained. The effects were greater for AF than for SO. However, inclusion of supplemental fat in diets decreases eggshell thickness and egg yolk yellowness, possibly due to a reduction in Ca absorption and intake of egg yolk colorants.

• Korean Journal of Poultry Science
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• v.33 no.2
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• pp.127-131
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• 2006

This study was conducted to investigate the effects of expanding feed processing technologies on the egg characteristics and nutrient digestibility in laying hens. A total of two hundred seventy laying hens were randomly allocated into three treatments with fifteen replications for six weeks. Dietary treatments include 1) MS(mash), 2) EC(expanded crumble) and 3) EP(expanded pellet). In the egg production, the EC and EP treatments were significantly higher than MS treatment(p<0.05). However, no significant difference was founded in egg weight(p>0.05). Egg shell breaking was increased in EC and EP treatments compared with MS treatment(p<0.05), however, egg shall thickness showed no significant difference(p>0.05). Egg production and egg shell breaking were significantly higher in EC and EP treatments than MS treatment(p<0.05), but egg weight and egg shell thickness were no significantly difference among treatment(p<0.05). Yolk color was increased in EC treatment compared with other treatments(p<0.05). Also, yolk index was significantly improved in EC and EP treatments compared with MS treatment(p<0.05). Haugh unit was increased in EC treatment compared with MS treatment(p<0.05). EC treatment had improved DM, Ca and P digestibility. In conclusion, Expanded crumble and pellet processing could improve the egg characteristic and nutrient digestibility in laying hens.

• Korean Journal of Poultry Science
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• v.32 no.4
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• pp.269-273
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• 2005

The wild ginseng culture by-products(WGCB) was added to the laying hens diets. A total of 420 Lohmann Brown laying hens were alloted to five levels of drinking water containing WGCB 0, 0.4, 0.8 1.6, $3.2\%$with seven replications and fed com-soybean meal based diets contained ME 2,800 kcal/kg, CP $16\%$. Laying Performance, egg quality, egg yolk fatty acid and cholesterol concentration were measured. Egg Production and daily e99 mass were significantly lower(p<0.05) in birds fed the WGCB $3.2\%$ compared with no WGCB group, but showed no significant effect in other treatments. Feed efficiency of birds fed $0.4\%$ WGCB showed the lowest of all treatments(p<0.05). Eggshell thickness was increased(p<0.05) by WGCB supplementation, whereas eggshell breaking strength, haugh unit and yolk color index were not affected. No significant change in yolk fatty acids and cholesterol were observed by the addition of WGCB.

• The Korean Journal of Malacology
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• v.23 no.2
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• pp.209-216
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• 2007

The ovarian structure and ultrastructural changes in germ cells during oogenesis of the spiny top shell, Batillus cornutus, have been investigated by light and electron microscopic observations. Specimens of the spiny top shell were collected from the subtidal zone of Wando, south coast of Korea. Spiny top shell was dioecious. The ovary was located on the surface of the hepatopancreas in the spiral posterior region. The ovary had greenish color in the gonadal mature season. The ovary comprised many ovarian lobules, and the ovarian lobule consisted of connective mesenchymal tissue and epithelial cells. Oogonium had a large nucleus with nucleolus of high electron density. In previtellogenic oocyte, small yolk granules of low electron density were scattered in the cytoplasm. Oocytes in the initial vitellogenic stage were connected with the ovarian lobule by egg stalk. The result of TEM observations showed that initial vitellogenic oocyte contained well-developed Golgi complex, endoplasmic reticula, tubular mitochondria and numerous yolk granules of various electron densities and sizes. The electron density, size and quantity of yolk granules which were distributed in the active vitellogenic oocyte were increased compared to the previous stage. Thickness of egg envelope in the late active vitellogenic oocyte was approximately 4.4 $[\mu}m$. Cytoplasm of ripe oocyte was filled with proteid yolk globules of high electron density and lipid yolk globules of low electron density. In this stage, the thickness of egg envelope was approximately 6.5 ${\mu}m$.

• Korean Journal of Poultry Science
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• v.45 no.4
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• pp.245-252
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• 2018

This study investigated the effect of LED light wavelength (color) on reproductive hormones and egg production of brown laying hens raised on floor. Red, blue, green and white colors of LED light were four treatments with four pens per treatment. One hundred forty four Hy-line brown laying hens (47 wks old) were allocated in a floor pen for six weeks trial. Egg production, egg quality, yolk cholesterol and hormones ($17{\beta}$-estradiol, progesterone) concentrations in plasma and oviduct were analyzed. Egg production of red group was higher (P<0.01) than that of green group. Haugh unit of eggs from red group was higher (P<0.01) than that of blue and green groups. Egg weight of green group was heavier (P<0.05) than that of red group. Shell of blue group was stronger (P<0.05) than that of red and white groups. Shell color of white group was browner (P<0.01) than that of blue and green groups. Yolk cholesterol of red group was higher (P<0.01) than that of others. Plasma $17{\beta}$-estradiol of red group was higher (P<0.05) than that of others at $3^{rd}$ week, but that of white group was highest (P<0.05) at $6^{th}$ week. Oviduct progesterone of green group was higher (P<0.01) than that of others. The result showed that the LED colors affect the reproductive hormone concentrations, egg production, egg weight and egg quality. This study suggested that red LED would be the most appropriate color for floor raising brown laying hens to sustain the egg production when it begins to decline with aging.

• Korean Journal of Organic Agriculture
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• v.12 no.2
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• pp.219-230
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• 2004

In recent years, the consumption of livestock products were markedly decreased by awareness of world-widely occurred diseases including mad cow disease, Foot and mouth disease, Hog cholera, and Poultry Influenza virus. the consumers ara also wanting to have highly safe food such as organic animal products because of concerning about residual of antibiotics in animal products. However, disease control and impairment of productivity are the major problem in organic animal production. On these points of view, the present study was undertaken to investigate the effects of 1% or 2% of dietary probiotics fortified with various minerals on improvement of egg production and egg quality in old lay6r feeding low quality feed mainly composed of food waste, sesame meal, and rice bran. After 4 weeks of experimental feeding, the diameter of spreading of egg white was clearly decreased from 11.2cm of control eggs to 10.5m and 10.1m in 1% and 2% treatment group eggs, respectively. The color of egg yolk was 9.3 in control eggs but remarkably increased in treatment groups showing 10.1~10.2. Egg production was 75.8% in control layers but significantly increased to 79.8% of 1% treatment group and 82.9% of 2% treatment group layers. Egg weights (C : 66.3g, 1% : 73.2g, and 2% : 76.7g) and egg shell thickness (C : 0.33mm, 1% : 0.35mm and 2% : 0.36mm) were also increased by the addition of 1% or 2% of probiotics when compared to those of control group eggs. All together, it has been suggested that dietary addition of probiotics fortified with various minerals can improve the egg quality and egg production in layer's productivities by the recycling of organic waste resources such as food waste and agricultural by-products.

• Korean Journal of Poultry Science
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• v.32 no.4
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• pp.275-279
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• 2005

This study investigated the effect of dried food-waste diets (FW) fermented by Aspergillus oryzae (AEW), on egg Production and egg qualify in laying hens. One hundred sixty eight laying hens, 50 week old, ISA-Brown wtrain were randomly distributed into 7 treatments with 3 relpicates of 8 birds per replicate and fed with standards laying hen diet replaced with FW and AFW at die=rent level Basal Diet, 25, 10, $75\%$, respectively. There was a significant decrease in feed intake for FW $50\%$ diet and egg Production was statistically lower in all W diet treatments(p<0.05). Water consumption was increased as the replacement increased. Egg weight, egg shell thickness and strength were similar among treatments. However, egg yolk color and Haugh unit showed distinct differences when W were added to their diets(p<0.05). No difference was observed in egg yolk cholesterol. It seems that FW diet could decrease the egg Production, but would be able to improve egg Quality. From this results, AO seemed applicable to laying hen diet as supplements.

• Food Science and Preservation
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• v.13 no.6
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• pp.714-719
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• 2006

The effect of diet with Laminaria religiosa on egg quality were investigated. Isa Brown chicken (28 weeks old) were raised for 10 week. The addition level of Laminaria religiosa to each treatment was 0, 10 and 20%, respectively. No difference in egg yolk and white color was found among treatment. The contents of Ca, Fe, K and Mg were increased but of P decreased with the feeding of Laminaria religiosa. Total mineral content were increased from 860.9 mg% to 936.8 mg%. Tensile strength of egg shell was improved from $63g/cm^2$ to $77g/cm^2$. Total cholesterol content were reduced from 1,760 mg% to 1,113 mg%. Among the analyzed amino acids, the content of each amino acid was reduced except histidine increased from 2,024.0 mg% to 2,119.6 mg%. Essential amino acids in the total amino acids, 42.1% to 42.6%, were hardly changed among treatments.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.3
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• pp.263-269
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• 2003

Morphological development of eggs, larvae and juvenile of the sunrise sculpin, Pseudoblennius cottoides were studed in the field and laboratory at the coastal Dolsan-do, Yeosu-shi from October, 2000 to April, 2001 Egg mass of Pseudoblennius cottoides in peribranchial cavity of Halocynthia hilgendorfi, were observed during late fall to winter in the study area. Fertiliged eggs were spherical in shape, demersal, adhesive, transparent and greenwish yellow color, measuring 1.84 mm (1.83-1.87 mm) in diameter. There were numerous and 17 (15-20) various-sized oil globules accounted in the yolk. Granular materials formed a mass in the yolk. Fertiliged eggs hatched at 301 hr 20 min after morula stage. Newly hatched larvae 6.31 mm (6.24-6.37 mm) in total length (TL), had a large yolk. At 3 days after hatching, the larvae, 6.77 mm (6.69-7.14 mm) in TL came out through the excurrent siphon of Halocynthia hilgendorfi. At 13 days after hatching, the larvae 12.59 mm (12.42-12.63 mm) in TL transformed to postlarval stage. At 32 days after hatching, postlarvae of TL 19.18 mm (19.01-19.46 mm) have reached the juvenile stage.

• Korean Journal of Poultry Science
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• v.49 no.2
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• pp.53-60
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• 2022

This study investigated correlations between egg quality indicators to identify external egg quality traits to predict internal egg quality using non-destructive and convenient measurements. Thirteen indicators, including Haugh unit, albumen height, eggshell breaking strength, eggshell thickness, eggshell color (CIE L^*, CIE a^*, CIE b^*), and reflectivity value, egg weight, egg length, egg width, shape index, and yolk color, were investigated. A total of 180 brown eggs were obtained from one 27-week-old flock of Hy-line brown-laying hens raised in a cage system. Correlations were evaluated using Pearson's correlation coefficient (r). The results showed strong correlations between Haugh unit and albumen height, eggshell color CIE L^* and reflectivity, egg weight and width, egg weight and length, eggshell color CIE L^* and CIE a^*, eggshell color CIE a^* and reflectivity, and shape index and egg length (P<0.001). Moderate correlations were observed between eggshell breaking strength and eggshell thickness, eggshell color CIE a^* and CIE b^*, and shape index and egg width (P<0.001). Eggshell color CIE L^* was correlated with eggshell breaking strength (P<0.01), and eggshell color CIE a^* was correlated with Haugh unit, albumen height (P<0.01), and eggshell breaking strength (P<0.001). The present study showed significant correlations between eggshell color and other quality indicators. Thus, this study suggests that eggshell colors based on reflectiveness and the CIE L^*a^*b^* value can be used to estimate the Haugh unit, albumen height, eggshell breaking strength, and thickness.