• 한국어류학회지
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• 제34권2호
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• pp.86-95
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• 2022

줄가자미의 난발생 및 자치어 형태발달을 관찰하여 종자생산 및 분류학적 연구의 기초자료로 이용하고자 한다. 2017~2018년까지 동해안에서 어미를 채집하여 원형수조(Ø 6 ×1 m) 수온 12.8±1.9℃에서 사육하였다. 수정란의 크기는 1.42~1.59 mm (평균±SD, 1.51±0.04 mm, n=50)였고, 난의 형태는 구형의 무색 투명한 분리부성란이었다. 난발생 과정은 수정 60분 후 난황과 난막이 분리되었고, 62시간 후 배체가 형성되었다. 수정 144시간 후 전체의 50% 이상이 부화가 완료되었다. 부화 직후의 자어는 전장 4.22~4.64 mm (4.53±0.16 mm, n=10)로 입과 항문은 열리지 않았고, 부화 후 10일째 전기자어는 전장 5.88~6.62 mm (6.31±0.33 mm, n=10)로 난황 흡수가 완료되고, 입이 열리기 시작하였다. 부화 후 55일째 중기자어는 전장 10.4~13.3 mm (12.7±1.3 mm, n=10)로 척추말단 끝부분은 휘어졌다. 부화 후 120일째는 전장 35.3~40.5 mm (39.5±2.4 mm, n=10)였고, 각 지느러미 기조 수는 등지느러미 79~94개, 뒷지느러미 63~75개로 정수에 달하면서 치어기로 이행하였다.

• The Korean Journal of Physiology
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• 제24권1호
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• pp.15-26
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• 1990

Hemoglobin was purified from the outdated human red blood cells. Phospholipids were purified from egg yolk and stored in chloroform. The artificial red blood cells (hemosome) were prepared by encapsulation of hemoglobin with phospholipid mutilayer using rotary vacuum evaporator. The shape and size of hemosomes were measured by phase contrast microscope and image analyzer. The function of hemosomes was tested by measuring oxygen dissociation curve using blood gas analyzer. In order to test whether hemosomes are useful as blood substitute they were infused into rats of which one third of total blood were drawn. The results obtained are summarized at followings. 1) Hemosomes were spherical shape and their mean diameter was 0.7 um. 2) Oxygen dissociation curve of hemosomes showed the same figure as that of normal red blood cells. 3) All rats given 1/3 transfusion with hemosomes survived until sacrificed whereas three of four rats given 1/3 transfusion with saline died within 1 hour and the rest of them died within 24 hours.

• IEIE Transactions on Smart Processing and Computing
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• 제3권1호
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• pp.28-34
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• 2014

Recently, ultrasound therapy has become a new and effective treatment for many brain diseases. Therefore, skull-mimicking phantoms have been developed to simulate the skull and brain tissue of a human and allow further research into ultrasound therapy. In this study, the suitability of various skull-mimicking materials(HDPE, POM C, Acrylic) for studies of brain-tumor treatments was evaluated using focused ultrasound. The acoustic properties of three synthetic resins were measured. The skull-mimicking materials were then combined with an egg white phantom to observe the differences in the ultrasound beam distortion according to the type of material. High-intensity polyethylene was found to be suitable as a skull-mimicking phantom because it had acoustic properties and a denatured-area shape that was close to those of the skull,. In this study, a skull-mimicking phantom with a multi-layer structure was produced after evaluating several skull-mimicking materials. This made it possible to predict the denaturation in a skull in relation to focused ultrasound. The development of a therapeutic protocol for a range of brain diseases will be useful in the future.

• 한국가금학회:학술대회논문집
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• 한국가금학회 2003년도 제20차 정기총회 및 학술발표회
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• pp.133-134
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• 2003

본 연구결과에서 개발 가공사료급여 시험구와 관행사료 급여구의 산란율과 부화율에서 유의적인 차이를 보이지 않고 있는 점은 관상조류에 개발 가공사료의 이용이 가능한 것으로 판단되었다. 다만 이를 이용하기 위해서는 조류의 사료 섭취습성을 이용한 가공사료의 형태적 측면을 고려하여야 할 것으로 사료되며, 새로운 사료에 대한 적응 및 길들이기를 위해서는 추가적인 연구가 필요한 것으로 판단된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제1권2호
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• pp.111-114
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• 2000

The female pupae of the silkworms Bombyx mori, were injected with recombinant Autographa californica nuclear polyhedrosis virus (AcNPV) expressing green fluorescent protein (GFP) by percutaneous inoculation. When the 4 day-old female pupae were injected with 1x10$^{7}$ or 2${\times}$10$^{7}$ plaque forming units (pfu) of the recombinant AcNPV, oviposited number and egg weight were significantly decreased. Furthermore, the shape of the eggs was obviously divides into normal and abnormal shapes. The percentage of the eggs with an abnormal shape was 7.8% and 57.1% at 1${\times}$10$^{7}$ and 2${\times}$10$^{7}$ pfu inoculation, respectively. PCR analysis of the genomic DNA extracted from the eggs revealed that gfp and AcNPV ecdysteroid UDP-glucosyltransferase genes were amplified from both types of eggs with normal and abnormal shapes. The results demonstrate that AcNPV DNA, and gfp gene cloned into the AcNPV genome, injected in pupal stage were transmitted to eggs and remained stable through at least next generation.

• 수산해양기술연구
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• 제43권3호
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• pp.192-205
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• 2007

The objective of this study was to investigate the optimal shapes and arrangements of sinkers attached to net cages to prevent their deformation in a current. A series of model experiments were conducted in a circulating water channel, using 5 different types of sinker(high-weighted ball, low-weighted ball, columntype, egg-shaped and iron bar-framed) and 2 types of square net cage constructed from both Nylon Raschel netting and Nylon knotted netting, on a 1/20th scale. The deflection of the model nets against the flow was smallest with the iron bar-framed weight compared to the other four types of sinker. It was expected that the optimal shapes of sinkers would be either the ball or egg-shape; however, iron bar-framed weight actually had larger drag forces. The dispersed deployment of sinkers on the bottom frames of model net cages performed better with relatively slow flows, while the concentrated deployment at 4 corners functioned better with relatively fast flows, in preventing the nets from becoming severely deformed. The deformation of the net cages was larger for the Nylon knotted netting than the Nylon Raschel netting. With respect to flow resistance, the Nylon Raschel netting, rather than the Nylon knotted netting, was more suitable for construction of net cages.

• 한국발생생물학회지:발생과생식
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• 제20권1호
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• pp.31-40
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• 2016

This study was conducted in order to examine the egg development in red spotted grouper, Epinephelus akaara and the morphological development of its larvae and juveniles, and to obtain data for taxonomic research. This study was conducted in June 2013, and 50 male and female fish were used for the study. One hundred ${\mu}g/kg$ of LHRHa was injected into the body of the fish for inducing spawning, and the fish were kept in a small-sized fish holder ($2{\times}2{\times}2m$). Eggs were colorless transparent free pelagic eggs, 0.71-0.77 mm large (mean $0.74{\pm}0.02mm$, n=30), and had an oil globule. Hatching started within 27 h after fertilization. Pre-larvae that emerged just after hatching were 2.02-2.17 mm in total length (mean $2.10{\pm}0.11mm$), their mouth and anus were not opened yet, and the whole body was covered with a membrane fin. Post-larvae that emerged 15 days post hatching were 3.88-4.07 mm in total length (mean $3.98{\pm}0.13mm$), and had a ventral fin with two rays and a caudal fin with eight rays. Juveniles that were formed at 55 d post hatching, were 31.9-35.2 mm in total length (mean $33.6{\pm}2.33mm$), with red color deposited over the entire body, and black chromophores deposited in a spotted pattern. The number of fin rays, body color, and shape were the same as that in the adult fish.

• The Korean Journal of Ecology
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• 제28권5호
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• pp.281-286
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• 2005

A. signifer의 성숙난은 타원형이며 동물극쪽이 볼록하게 부푼 모양이다. 수정란의 크기는 장경이 $2.19{\pm}0.014mm$, 단경이 $1.85{\pm}0.080mm$로 장경이 단경보다 긴 모양이며 유구(oil globe)는 관찰되지 않았다. A. signifer의 방사대(zona radiata)는 그물과 같이 규칙적인 섬유성 그물(fibrilar net)구조로 되어 있었으며, 외측구의 직경이 약 $2.5{\mu}m$정도이고 내측구는 약 $1.5\sim2{\mu}m$이었다. A. signifer의 수정란은 $20{\pm}1^{\circ}C$하에서 동물극쪽에서 배반엽이 일정한 각도로 기울어져 발생이 진행되었으며 49시간 만에 부화하였고, 부화후 21일이 경과하여 난황이 모두 흡수되었다.

• 한국수산과학회지
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• 제36권3호
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• pp.263-269
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• 2003

Morphological development of eggs, larvae and juvenile of the sunrise sculpin, Pseudoblennius cottoides were studed in the field and laboratory at the coastal Dolsan-do, Yeosu-shi from October, 2000 to April, 2001 Egg mass of Pseudoblennius cottoides in peribranchial cavity of Halocynthia hilgendorfi, were observed during late fall to winter in the study area. Fertiliged eggs were spherical in shape, demersal, adhesive, transparent and greenwish yellow color, measuring 1.84 mm (1.83-1.87 mm) in diameter. There were numerous and 17 (15-20) various-sized oil globules accounted in the yolk. Granular materials formed a mass in the yolk. Fertiliged eggs hatched at 301 hr 20 min after morula stage. Newly hatched larvae 6.31 mm (6.24-6.37 mm) in total length (TL), had a large yolk. At 3 days after hatching, the larvae, 6.77 mm (6.69-7.14 mm) in TL came out through the excurrent siphon of Halocynthia hilgendorfi. At 13 days after hatching, the larvae 12.59 mm (12.42-12.63 mm) in TL transformed to postlarval stage. At 32 days after hatching, postlarvae of TL 19.18 mm (19.01-19.46 mm) have reached the juvenile stage.

• The Korean Journal of Ecology
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• 제1권2호
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• pp.44-48
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• 1977

The spawning behavior and developmental pattern of the Korean eleotrid fish (Odontobutis obscurus) was investigated in one of the tributary of Han River. The spawining of this species begins on April and continues up to August, whereas, the spawning. of same species were between the end of June and the middle of July in Kyushu, Japan. August The spawning places were the margin of river, its the bottom composed of sand and pebble (Table 5). The velocity of water in the spawning places was 0-40cm/sec, and the depth of waters was 10-40cm. The egg was spawned on underside of stone in the spawning places with one or two folds and ellipsoid in shape, 3.4mm in long diameter and 2.0mm in short diameter. The yolk is 1.2mm in diameter and yellow in color. For hatching of the egg, it takes one month in, $16^{circ}C~19^{\circ}C$ water, in Japan, whereas it takes only 14 days in $20^{circ}C~30^{\circ}C$ wate rin the study area. The growth rate of larva and juvenile fish were as follows: new hatched larva is 45mm in length; after 5 days it became 5.0 to 5.5mm in length and 1.2 to 1.5mm in height after 20 days. it became 11.0mm in length and 2.3mm in length. The mean size of the mature female is 110mm in total length and larger 10mm than that of the same fish found in Japan.