• Fisheries and Aquatic Sciences
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• v.8 no.4
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• pp.228-234
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• 2005

Despite the relatively high production of fingerlings of the olive flounder Paralichthys olivaceus, its larval rearing in terms of first feeding has not been fully analyzed. We evaluated the variations of amino acids and fatty acids of starved larvae over 96 hr after hatching. We also investigated depletion of the yolk and oil globule of starved larvae and those fed the rotifer Brachionus plicatilis. In addition, the optimum size of the rotifers according to the mouth size of the larvae, and the point of no return with delay of the first feeding, were also examined. The amino acids in the egg decreased abruptly during embryo development. At 48 to 72 hr after hatching, the amino acids of starved larvae decreased by $30-40\%$ from the level in newly hatched larvae. The concentrations of fatty acids in newly hatched larvae were lower than those of floating eggs and dropped sharply at 48 hr after hatching, when the yolk disappeared. The starved larvae depleted their yolksacs and oil globules earlier than the fed larvae did. At 84 hr after hatching, rotifers were detected for the first time in the guts of the larvae, which were about 3 mm in total length. The point of no return appeared to be close to the fourth day from the first feeding. For a high survival rate of P. olivaceus larvae, the first feeding should occur before the third day after hatching.

• Korean journal of applied entomology
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• v.32 no.1
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• pp.83-89
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• 1993

This study investigated seasonal occurrence, host preference and developmental characteristics of E. lagerstroemiae was most likely to have two generations a year with first occurrence of adults form late April to late may and second from late August to late October including 2 peaks in early May and late August, respectively. Overwintering stages were mostly eggs and larvae and its composition rate was 57.1% of eggs and 42.9% of larvae. Host plats investigated were 7 species 7 families and most preferred species were Lagerstroemia japonica and Diospyros kaki. As morphological characteristics of E. lagerstroemiae, sized of length/sidth were 0.29/0.16 mm for the egg. 0.41/0.16 mm for the first instar larva, 0.96/0.47 mm for the male adult. Number of eggs deposited per female adult was 221.9 individuals. Hatching rate at various temperatures was above 90% at below $30^{\circ}C$. Egg periods were also short\ened with increase of temperatures at below $30^{\circ}C$ but decreased to 56.3% at $35^{\circ}C$. Egg periods were also shortened with increase of temperatures at below $30^{\circ}C$. Hatching rate was not affected by photoperiods but egg periods were shortened with increase of day length.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.73-76
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• 2004

The rate of oxygen consumption of the silkworm eggs was measured to set up the barometer for measuring the effect of egg chilling on diapause termination. In diapause eggs, $O_2$ uptake showed a maximum of 79.2 ${mu}ell$/mg eggs/hr, at one day after oviposition and then gradually decreased to 2.2${mu}ell$/mg eggs/hr at 9days. The rates of oxygen uptake of eggs raised immediately after HCl-treatment and reached to a maximal level of 484.5 ${mu}ell$/mg eggs/hr in 9-day-old eggs, which corresponds to 220 fold that of diapause eggs, and the hatching ability was also over 50％. In order to break diapause, eggs incubated at $25^{\circ}C$ for 30 days after oviposition had to be kept at least for 45 days at 5$^{\circ}C$, but chilled eggs for 90 days or longer hatched at 10 days as normal hatching periods. We also investigate periods which the value of $O_2$ uptake of eggs chilled during 15 to 120 days at 5$^{\circ}C$ reached at 200 ${mu}ell$/mg eggs/hr. As a result of that, $O_2$ uptake of eggs chilled during 15 to 45days were not reached at 200 ${mu}ell$/mg eggs/hr and longer the chilling durations are, the higher the rate of $O_2$ uptake is and the longer the chilling durations are, the shorter the periods up to hatching is. And also, hatch-ability increased rapidly as $O_2$ uptake reaches over 20/eggs/hr.

• Korean Journal of Poultry Science
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• v.12 no.1
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• pp.1-6
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• 1985

To secure a higher percentage of fertility in hatching eggs and a higher percentage of hatchability of the fertile eggs should be of direct benefit to poultry breeder ana hatchery operators. This study was carried out to estimate the relationships of egg production and fertility in broiler breeder. The data analysed were the record of pullets (5 strains) of broiler breeder raised at Poultry Breeding Farm, Seoul National Univ. from 1982 to 1983. Since the purpose of the study was only to find out the relationships between egg Production and fertility, many other factors thought to be included in fertility were fixed as much as possible. The results were summarized as follows. 1. Mean figure of egg production rate and fertility from 30 weeks age to 60 Weeks of age to 60 weeks of age were 60.5-65.2 percent and 82.7-87.9 percent. 2. Correlation coefficients between egg Production rate and fertility were high and positive, except line K, and the coefficients have highly significant differences. 3. Regression coefficients of fertility on egg Production rate were 0.54-0.97, except line K. 4. Maximum fertility age estimated from the polynomial regression equation were 44-47 weeks. The results obtained in this study suggested that the selection of high performance strain in egg Production should improve the fertility.

• Ocean and Polar Research
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• v.40 no.3
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• pp.161-167
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• 2018

We examined the effect of salinity and water temperature on hatching and survival rates of fertilized eggs of hybrid grouper (Epinephelus fuscoguttatus ♀ ${\times}$ E. lanceolatus ♂) at different developmental stages, determining optimal conditions for their long-distance transportation. Deformities and hatching rates of fertilized grouper eggs were observed at salinities of 24, 27, 30, 33, 36, and 39 psu. The optimal salinity was determined to be 36 psu, with a survival rate of $70.0{\pm}2.0%$. Transportation experiments at 36 psu were conducted at water temperatures of 21, 24, 27, and $30^{\circ}C$, different developmental stages such as morula, 5-myomere, and tail beating for hatching and survival rates. The optimal water temperature and developmental stage for transporatation were $30^{\circ}C$ and tail beating stage and those hatching rates were $50.6{\pm}1.9%$ and $86.3{\pm}1.3%$, respectively. At $21^{\circ}C$, the survival rate by transportation water temperature was highest ($73.1{\pm}10.6%$), but the hatching rate ($17.1{\pm}3.1%$) was lowest. Therefore, the hybrid grouper fertilized eggs (E. fuscoguttatus ♀ ${\times}$ E. lanceolatus ♂) can be most efficiently produced under long-distance transportation conditions during the tail beating stage and at a water temperature of $30^{\circ}C$.

• Journal of Aquaculture
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• v.12 no.1
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• pp.39-45
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• 1999

In order to obtain the basic biological data for artificial seedling production of sea cucumber, Stichopus japonicus, the influence of water temperature and air dry for spawning induction, egg development and larvae rearing was investigated during the period from April, 1995 through September, 1995. Spawning induction rate by the water temperature was 6.0~17.5% and air dry was responsed 1.4~4.0%. Number of eggs spawned of Stichopus japonicus were $50~500\times10^4$ individuals, the fertilization and hatching rate were ranged 84.0~96.0%, 71.4~84.6% respectively. The fertilized egg of Stichopus japonicus appeared mean diameter of $154{\mu}m$. At a constant water temperature of $23^{circ)C$, it become 4 tell stage from fertilization after 2 hours 10 minutes, hatching larvae after 14 hours half, auricularia larvae after 3 days, doliolaria larvare after 11 days and pentactula larvae after 15 days ready for settlement. The suitable food in the larvae reared for 17 days after fertilization were shown the best growth and survival in the larvae food of Chaetoceros calcitrans. Optimum density for larvae rearing were maintained of the larval density lower than 2 individuals/ml.

• Journal of Marine Life Science
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• v.7 no.2
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• pp.139-144
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• 2022

This study aims to investigate egg quality changes and free amino acid composition. Factors that can affect egg quality about farming conditions were investigated through biochemical analysis of egg by red spotted grouper, Epinephelus akaara. As a result, the buoyant, fertilization embryonic survival and hatching rate were better when reared in sea cage than in tank. And the content of free amino acid in fertilized eggs reared in sea cage was higher then in tank. In conclusion, fertilized eggs with good egg quality can be produced when farming sea cage, and contained more free amino acid.

• Korean Journal of Ichthyology
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• v.34 no.1
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• pp.25-33
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• 2022

We investigated to elucidate the early life history of siberian stone loach, Barbatula nuda (Pisces: Nemacheilidae). Adult fish were collected using net in the Sangcheoncheon Stream, Cheongpyeong-myeon, Gapyeong-gun, Gyeonggi-do, Korea, 26th April 2020. HCG 10 IU/g was injected to female and male fish and fertilized by dry method in the laboratory. The number of eggs of B. nuda was 1,308±293 (TL: 70.64~79.24 mm), and the egg color was yellowish grey. Fertilized eggs measured 1.13±0.01 mm (n=10) in diameter and showed adhesion. The fertilized eggs started hatching after 104 hours. At the time of egg development, the 15℃ group showed an average hatching rate of 80.1±1.2% and the 20℃ group showed 61.5±6.8%, so the 15℃ group was suitable (p<0.05). Newly hatched larva an average were 3.12±0.06 mm (n=10) in total length. At 4 days after hatching, the total length was 4.89±0.09 mm (n=10), and feeding began with the mouth and anus opened, and entered to preflexion larvae stage. At 21 days after hatching, the total length was 10.98±0.40 mm (n=10), and the distal part of the notochord was bent to 45°, and entered to postflexion larvae stage At 26 days after hatching, the total length was 13.47±0.37 mm (n=10), and entered the juvenile stage.

• Korean Journal of Ichthyology
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• v.33 no.4
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• pp.262-272
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• 2021

The egg development and early life history of Tribolodon hakonensis that collected from the Seomjin River were studied. The fertilized eggs of the T. hakonensis were slightly adhesive and separated with a yellow yolk and no oil globule. The size of the eggs was an average of 2.89 mm (2.79~2.96) in diameter. Fifty percents of the embryos were hatched in about 63 hrs after fertilization at water temperature of 20℃. The newly hatched larvae showed average 6.92 mm (6.09~7.60) in total length. At 8 days after hatching, they were 13.75±0.67 mm (n=10) in total length and their yolk sacs were completely absorbed, opening mouth and anus (postflexion larvae stage). At 40 days after hatching, they became juvenile and reached 26.99±1.82 mm (n=10) in total length and all their fin rays were formed. At 137 days after hatching, the young fish were 72.63±10.7 mm (n=10) in total length and their body shape, color and behavior were similar to those of adult fish. The size of eggs and the larva after hatching significantly affected the initial growth among groups of genus Tribolodon, and the hatching time of fertilized eggs and the growth rate of the early life history according to the water temperate were different.