• Applied Microscopy
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• 제50권
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• pp.13.1-13.7
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• 2020

We examined the morphology of fertilized egg and ultrastructures of fertilized egg envelopes of Ancistrus cirrhosus belong to Loricariidae using light and electron microscopes. The fertilized eggs formed a mass on the spawning place and were yellowish, spherical, non-transparent, demersal, adhesive, and a narrow perivitelline space. But, the adhesiveness of fertilized eggs was disappeared after spawning excluding contact parts. The micropyle with funnel shape was surrounded by 15-19 furrow lines of egg envelope in a spoke-like pattern. The outer surface of egg envelope has smooth side and inner surface of egg envelope was rough with grooves. Also, the total thickness of the fertilized egg envelope was about 32.58 ± 0.85 ㎛ (n = 20), and the fertilized egg envelope consisted of three layers, an outer adhesive electron-dense layer, a middle layer with low electron density and an inner electron-dense layer with grooves in counter structure from other most teleost. Collectively, these morphological characteristics and adhesive property of fertilized egg, and ultrastructures of micropyle, outer surface, and section of fertilized egg envelope are showed species specificity.

• 농업과학연구
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• 제29권1호
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• pp.44-54
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• 2002

이 실험은 우리나라 농가에서 사육되고 있는 타조(African ostrich) 신선란의 내 외부적인 품질 특성을 구명하고자 하였다. 타조의 난형은 장경 15.1~16.1cm, 단경 12.1~13.6cm로, 난형지수는 평균 81.0의 값을 나타내었고, 난각색은 계란보다 광택이 좋은 백색을 나타내었다. 계산식에 의한 난각강도와 난표면적 은 각각 $41.155kg/cm^3$과 $804.7cm^2$이었다. 타조 난의 난각두께는 난각막을 포함하여 1.89mm로, 각 부위별 난각두께는 거의 비슷한 값을 나타내었으며, 난각막 두께는 0.10mm로, 적도부가 두껍고 둔단부가 상대적으로 얇은 것으로 나타났다. 난각 기공수는 19.77개/$cm^2$으로, 첨단부의 기공수가 대체로 많은 것으로 나타났으며, 1개 알의 총기공수는 약 15241개 정도로 측정되었다. 타조 알의 각 부위의 난중에 대한 비율은 난황 25.1%, 난백 58.2% 그리고 난각은 16.7%이었고, 난백에 대한 난황의 비율(Y/W)은 0.43을 나타내었다. 타조의 알에 대한 난백과 난황의 품질을 조사한 결과 난액에 있어 직경과 높이는 각각 19.7cm 와 13.2mm로 난백지수는 0.07 이며, H.U는 -69.7, pH는 8.3이었으며, 난황에 있어서는 직경이 14.4 cm, 높이 29.4 mm로 난황지수는 0.20으로 나타났고, pH는 6.6 이었다. 또한 난황색은 두터운 난황막으로 인하여 계란보다는 엽은 황색을 보였다.

• 한국식품영양학회지
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• 제30권2호
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• pp.269-281
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• 2017

In this study, quality characteristics and antioxidative activities were comparatively evaluated between the macaron with egg white powder added at the ratios of 2, 4, 6 and 8% and the macaron with added liquid egg white, in order to determine the applicability of the macaron with added egg white powder. The analysis of amino acids indicated that the group with 8% of egg white powder added showed the highest total amino acid content of 7,584.20 mg/100 g. The group with 4% of egg white powder added showed the highest sweetness (p<0.01); whereas, and the group with 2% of egg white powder added showed the highest pH (p<0.001). In terms of the chromaticity, L (p<0.01) and a values (p<0.05) were increased with the increasing percentage of added egg white powder. With the increasing percentage of added egg white powder, textures such as hardness (p<0.01), chewiness (p<0.05), gumminess (p<0.001), and cohesiveness (p<0.001) decreased; whereas, adhesiveness (p<0.001) and springiness (p<0.01) increased. The results of the sensory evaluation indicated the highest preference for the macaron containing 6% white egg powder, in all items. DPPH free radical scavenging activity, SOD-like activity, and reducing power were increased with increasing amounts of added egg white powder (p<0.001). Collectively, the results indicated that the production of macaron with the addition of 6% of egg white powder is the most suitable mixing ratio for enhancing the preference and quality level.

• 한국지반환경공학회 논문집
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• 제3권2호
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• pp.5-14
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• 2002

본 연구에서는 지중내에 설치한 매설관에 작용하는 응력-변형 특성을 분석하였다. 연약지반의 수축과 팽창특성을 고려할 수 있는 Egg-Cam Clay 모델을 소개하였다. 기존에 사용하던 Cam Clay 모델은 연약지반의 수축 및 팽창 특성을 고려하지 못하는 단점이 있기 때문에 Cam Clay 모델을 수정하여 Egg-Cam Clay 모델을 제안하였다. 이와는 별도로 지중내의 매설관에 작용하는 응력-변형을 감소시키기 위하여 지오그리드, EPS 지오폼을 매설관과 함께 설치하였다. 탄소성모델을 이용하여 현장에 시험시공한 여러 단면에 유한요소해석한 결과 긍정적인 효과를 얻었다.

• Applied Microscopy
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• 제47권2호
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• pp.70-74
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• 2017

In the case of genus Nothobranchius, Nothobranchiidae, the morphology of fertilized eggs and ultrastructures of fertilized egg envelopes have been reported in only two fishes. Therefore it is hard for sure to these morphological characteristics show genus specificity because of lower research samples. So, we studied the morphology of fertilized egg, and compared the ultrastructures of outer surface, micropyle, and section of fertilized egg envelopes under the light and electron microscopes from the other two species, Nothobranchius foerschi and Nothobranchius rachovii, Nothobranchiidae to find out whether these structures have the species specificity or not. Both fertilized eggs were spherical, demersal and adhesive, and have a large oil droplet. The adhesive whip-like structures were distributed on the outer surface of egg envelope, and a micropyle located on the animal pole. The fertilized egg envelope consisted of two distinct layers: an outer electron-dense layer with adhesive structures and an inner lamellae layer in both species. The external shapes of fertilized egg and ultrastuctures of outer surface, micropyle, and section of fertilized egg envelope have same structure including results before. Our data indicate that these morphological characteristics of fertilized egg and fertilized egg envelope show genus Nothobranchius specificity.

• Applied Microscopy
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• 제52권
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• pp.6.1-6.5
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• 2022

We examined the morphology of the fertilized egg and the fine structure of fertilized egg envelopes of Poropanchax normani belonging to the family Poeciliidae, also known as Norman's lampeye using light and electron microscopes. The fertilized eggs with narrow perivitelline space were found to be spherical and demersal, additionally containing small oil droplets in the vitelline membrane. Further, a bundle of adhesive filaments was observed to be present on one side of the fertilized egg. These filaments possessed remarkably high elasticity and were approximately 1-3mm in length. The size of the fertilized egg was determined to be about 1.49 ± 0.07mm (n=30). The outer surface appeared smooth, and adhesive filaments originating at different location of the surface of the envelope were found to be distributed around the egg envelope and were joined together to form a single long bundle in scanning electron microscopic observation. A peak-like structure formed of several straight wrinkles was observed around the micropyle. However, the complete structure of the micropyle could not be studied due to the depth at which it was located. Additionally, the total thickness of the egg envelope was ascertained to be approximately12.5-14.5㎛. The egg envelope consisted of two distinct layers, an outer electron dense layer and an inner lamellar layer, further consisting of 10 sublayers of varying thicknesses. Collectively, it was observed that the morphological characteristics of the fertilized egg, fine structures surrounding the micropyle, outer surface, adhesive structure consisting adhesive filaments, and sections of fertilized egg envelope displayed species specificity.

• 한국환경과학회지
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• 제26권7호
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• pp.869-874
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• 2017

This study was conducted to investigate the effects of dietary supplementation of various types of fermented red ginseng marc with red koji to laying hens on eggshell and egg yolk quality characteristics. A total of 240 Hy-line Brown laying hens (40 wk of age) were randomly allotted to 24 pens (6 replicates per treatment and 10 laying hens per replicate). Experimental diets consisted of 4 treatments containing basal diet (control), 1% fermented red ginseng marc powder with red koji, 1% fermented red ginseng marc pelleted with red koji and 1% fermented red ginseng marc coated with red koji. During the 8-wk feeding trial, there were no significant differences in eggshell strength, eggshell thickness and eggshell color among the treatments, except for eggshell strength at 4 and 8 wk and eggshell thickness at 0 wk. In addition, no differences in egg yolk color and egg yolk index were found for all treatments throughout the 8-wk feeding period, except for egg yolk color at 0 wk. Thus, using various types of fermented red ginseng marc with red koji to laying hens did not improve eggshell and egg yolk quality characteristics.

• 한국축산식품학회지
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• 제24권3호
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• pp.277-282
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• 2004

본 연구는 원적외선 저장 쌀의 급여가 산란계에 미치는 영향을 조사하기 위하여 수행되었다. 사육 기간 중 대조구에 비해 다소 원적외선 저장 쌀의 급여가 산란계의 체중에는 큰 영향을 미치지 않았으나 난중의 무게, 난황의 무게, 난각의 두께 및 산란율에 있어서는 각각 3.6∼7.6%, 3.9∼30.2%, 5.4∼13.5%및 5.4%의 증가율을 나타내었다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권8호
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• pp.1182-1185
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• 2002

The objective of this study was to investigate the effect of long light regime (20 h light and 4 h dark, 20L:4D) and short light regime (4 h light and 20 h dark, 4L:20D) on egg production and egg characteristics of laying geese. Thirty-six laying birds, 4 replicates of 3 birds per treatment were allotted to three light regimes, i.e., 20L:4D, 4L:20D, and natural light (NAT) from March 7 to June 20. Results showed that the geese in 20L:4D consumed 54 g less feed per goose daily and laid 17.5 less eggs per goose (p<0.05) comparing to those in 4L:20D. The number of days from initiation of light treatment till cease of laying was 22 days shorter (p<0.05) in 20L:4D comparing to that in NAT. Five geese (41.7%) in 4L:20D kept laying by the end of applying light regime. Weight and surface area of the eggs in 4L:20D were greater (p<0.05) comparing to those in the other two light regimes. It is concluded that the period of egg production in goose could be manipulated by light regime in the ways such as using short light regime of 4 h light daily to prolong egg production through summer and using long light regime of 20 h light daily to induce cease of egg production.

• Applied Microscopy
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• 제51권
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• pp.3.1-3.6
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• 2021

We examined the morphology of fertilized egg and ultrastructures of fertilized egg envelopes of dwarf rainbowfish (Melanotaenia praecox) belong to Melanotaeniidae using light and electron microscopes. The fertilized eggs were spherical with adhesive filament, transparent, demersal, and had a narrow perivitelline space and small oil droplets. The size of fertilized egg was 1.02 ± 0.18 mm (n = 30), and there were two kinds of adhesive filament on the fertilized eggs. The long and thick (diameter 12.22 ± 0.52 ㎛, n = 20) adhesive filaments were only at the area of animal pole, and short and thin (diameter 1.99 ± 0.23 ㎛, n = 20) adhesive filaments were around the long filaments. A micropyle was conical shaped with adhesive filament and located near the animal pole of egg. The outer surface of fertilized egg was rough side. Also, the total thickness of the fertilized egg envelope was about 7.46 ± 0.41 ㎛ (n = 20), the fertilized egg envelope consisted of two layers, an inner lamellae layer and an outer layer with high electron-density. And the inner layer was 8 layers. Collectively, these morphological characteristics and adhesive property of fertilized egg with adhesive filaments, and ultrastructures of micropyle, outer surface, and section of fertilized egg envelope are showed species specificity.