• Title/Summary/Keyword: egg and fry

Search Result 16, Processing Time 0.019 seconds

Development of a Species Identification Method for the Egg and Fry of the Three Korean Bitterling Fishes (Pisces: Acheilognathinae) using RFLP (Restriction Fragment Length Polymorphism) Markers (제한절편 길이 다형성(RFLP) 분자마커를 이용한 납자루아과 담수어류 3종의 난과 치어 종 동정 기법 개발)

  • Choi, Hee-kyu;Lee, Hyuk Je
    • Korean Journal of Environmental Biology
    • /
    • v.36 no.3
    • /
    • pp.352-358
    • /
    • 2018
  • This study aimed to develop a species identification method for the egg and fry of the three Korean bitterling fishes (Pisces: Acheilognathinae), including Acheilognathus signifer, Acheilognathus yamatsutae and Rhodeus uyekii based on the PCR-based Restriction Fragment Length Polymorphism (RFLP) markers. We conducted a field survey on the Deokchicheon River from the North Han River basin, where the three Acheilognathinae species co-occur, and also analyzed the existing sequence dataset available from the GenBank. We found coexistence of the three species at the study site. The egg and fry were obtained from the host mussels (Unio douglasiae sinuolatus) by hand from May to June 2015 and in May 2017. To develop PCR-based RFLP markers for species identification of the three Acheilognathinae fish species, restriction enzymes pinpointing species-specific single nucleotide variation (SNV) sites in mitochondrial DNA COI (cytochrome oxidase I) and cyt b (cytochrome b) genes were determined. Genomic DNA was extracted from the egg and fry and RFLP experiments were carried out using restriction enzymes Apal I, Stu I and EcoR V for A. signifer, A. yamatsutae and R. uyekii, respectively. Consequently, unambiguous discrimination of the three species was possible, as could be seen in DNA band patterns from gel electrophoresis. Our developed PCR-based RFLP markers will be useful for the determination of the three species for the young and would assist in studying the spawning patterns and reproductive ecology of Acheilognathinae fishes. Furthermore, we believe the obtained information will be of importance for future maintenance, management and conservation of these natural and endangered species.

Analysis of Voice Parameters on Different Phonatory Tasks using Multi-Channel Phonatory Function Analyzer in Healthy Adults (다채널 음성분석장치를 이용한 정상 성인에서의 발성 방식에 따른 음성변수 분석)

  • 성명훈;이상준;김광현;노종렬;권택균;이강진;박광석;최종민
    • Journal of the Korean Society of Laryngology, Phoniatrics and Logopedics
    • /
    • v.13 no.2
    • /
    • pp.132-138
    • /
    • 2002
  • Background and Objectives : The complex physiologic structure of the larynx can vibrate in three or more different ways that yield acuostically and perceptually distinct vocal quality. The purpose of this study is to examine the normal range of voice parameters in Multi-Channel Phonatory Function Analyzer and investigate the difference of voice parameters according to the phonatory patterns. Materials and Methods : Forty normal adult speakers (20 men and 20 women) with age ranging from third to forth decades pronounce low, comfortable, and high tone /a/ ; comfortable tone /${\ae}$/, /i/, /o/, and /u/ : fry, falsetto. Voice was analyzed by Newly developed multi-channel phonatory function analyzer. Results : The normal range of voice parameters in this system was similar to the existing data. Fry shows high jitter and falsetto low SQ. Fry and falsetto show low OQ in men but no difference in women. Jitter, OQ and SQ were different between men and women in modal register, whereas there was no gender difference in fry and falsetto. In frequency magnitude spectrum and EGG, modal register, fry and falsetto have distinguishing pattern. Conclusions : Modal register, fry and falsetto are distinguishable in voice parameters and show different vibratory patterns.

  • PDF

The Mass Production of Fertilized Eggs for Industrial Aquaculture of the Convict Grouper Hyporthodus septemfasicatus (능성어(Hyporthodus septemfasicatus)의 산업적 양식을 위한 수정란 대량생산)

  • Park, Chung-Kug
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.54 no.1
    • /
    • pp.31-37
    • /
    • 2021
  • The mass production of fertilized eggs of the convict grouper Hyporthodus septemfasciatus was studied from 2013 to 2020 for industrial aquaculture. The experiment was divided into two groups. Group 1 broodstock was raised from wild-caught fry and used from 2013 to 2020. Group 2 broodstock was raised from artificially propagated fry and used from 2019 to 2020. Males used to collect sperm for artificial insemination weighed more than 7 kg. The effects of various hormones on artificial ovulation were investigated from 2013 onward. Among these, luteinizing hormone-releasing hormone analogue (LHRHa) at 100 ㎍/kg body weight showed the most effective results and was used for artificial egg collection from 2014 onward. In Group 1, the average total egg production per year, average egg production per individual, fertilization rate, and hatching rate were 26,143 mL, 609.7 mL, 93.3%, and 91.8%, respectively, and in Group 2, were 2,750 mL, 316.5 mL, 92.1%, and 90.4%, respectively. Based on these results, we showed that a large number of fertilized eggs for artificial seeding could be produced consistently. Moreover, the mass production of fertilized eggs in Group 2 establishes a foundation for the complete aquaculture cycle of H. septemfasciatus.

Early-growth comparison of diploid and triploid rainbow trout (Oncorhynchus mykiss) in South Korea

  • Seung-Baek Lee;Josel Cadangin;Su-Jin Park;Youn-Hee Choi
    • Fisheries and Aquatic Sciences
    • /
    • v.26 no.7
    • /
    • pp.447-454
    • /
    • 2023
  • Fast somatic growth is important considerations for successful and competitive aquaculture industry. In rainbow trout reared in South Korea, triploid induction was used to suppress negative influence of reproductive maturation to body growth. However, the effects of triploidy are visible in both mature fish and developing juvenile fish. Thus, it is also important to explicate the effect of triploid induction on growth during the early-life stages of rainbow trout-alevins and fry. Rainbow trout fertilized eggs were subjected to triploid induction and polyploidy was checked by flow cytometry. Diploid and triploid alevins and fry were reared separately in tanks with constant flow of freshwater through flow-through water system and growth measurements were done from zero days after hatching (DAH 0) until DAH 134. The egg-yolk morphometrics of alevins-yolk length, yolk height, yolk volume and yolk weight-were statistically similar (p > 0.05) in both genotypes from DAH 0 to DAH 22. The total length, body height, and body weight of alevins and fry were statistically better (p > 0.05) in both genotypes until DAH 92 but thereafter, triploid had a significantly better growth performance (p < 0.05) over diploid fish until the completion of study at DAH 134. With that, triploid induction did not influence alevin yolk regions and body growth and fry somatic growth until around 3 months after hatching, but considerable growth enhancement was subsequently apparent.

Characteristics of Korean Stop Consonants by Using Electroglottography and Its Clinical Application (Electroglottography를 사용한 한국어 폐쇄자음의 특성 및 임상적 적용)

  • Chae, Y.J.;Kim, H.G.;Hong, K.H.
    • Speech Sciences
    • /
    • v.4 no.2
    • /
    • pp.157-177
    • /
    • 1998
  • An electroglottography (EGG) was used to investigate the function of the vocal folds during their vibration. In this study, four Korean native speakers and 10 vocal polyp patients were selected. To investigate the dynamic change of EGG waveforms for the three-way distinction of Korean stops, a DSP-Sona graph model 5500, a Rino- Laryngeal stroboscope, a CSL model 4300B and a Laryngograph were used. An EGG Model 4338 was used to exam the vocal polyp of patients' voices during high, low, comfortable pitch production. The purpose of this study is to investigate the characteristics of Korean stop consonants in relation to pitch and to observe laryngeal movement during vocal fold vibration and speech production. The basic data accumulated during this research can be applied in clinical treatment. The results are as follows: on the Korean stop consonants, the aspirated stop is the highest in the GOT and PC1. On the angle of vowel contour, the angle of lenis is smaller than the angle of heavily aspirated and glottalized stops. The fundamental frequency is lowest at the lenis stop, In vocal polyp patients', the low pitch range is smaller than in normal speakers'. The pitch break and the vocal fry were observed. The jitter and OQ value are higher in vocal polyp patients than in those of normal speakers'.

  • PDF

Egg Development and Juvenile Growth of the Pacific Cod Gadus macrocephalus (Korean East Sea Population) (한국 동해계군 대구 (Gadus macrocephalus)의 난발생과 자치어의 성장)

  • Seo, Young-Seok;Park, Mu-Eok;Kim, Jin-Gak;Lee, Sang-Uk
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.40 no.6
    • /
    • pp.380-386
    • /
    • 2007
  • Egg development and juvenile growth of the Pacific cod Gadus macrocephalus (Korean East Sea Population) were studied to increase fry production using mass cultivation. The eggs were rectangular and adhesive sinking type. The egg size and fertilization rate were 1.01-1.09 mm and 72.4%, respectively. The cumulative times for egg hatching at 3, 6, 9, and $12\;^{\circ}C$ were 600, 360, 240, and 192 hrs, respectively. The hatching rates at 3, 6, 9, and $12\;^{\circ}C$ were 60.2, 68.9, 62.5, and 40.6%, respectively. After 11, 45, and 90 days, the larvae grew to a total length of 5.46-5.99, 9.42-10.06, and 23.0-32.0 mm, respectively. At 100 days from hatching, they grew to an average of 30 mm with a 7.1 % survival rate. By 312 days, juveniles with a total length of 3.6 cm grew to a total length of 14.7-20.1 cm and a body weight of 38.4-73.9 g. The specific growth rates of total length and body weight of the juveniles were 0.50% and 1.12%, respectively.

Morphological study of Oncorhynchus spp. (Pisces : Salmonidae) in Korea- I Egg development and morphology of alevin, fry and smolt of chum salmon, Oncorhynchus keta. (한국산 연어속 어류의 형태학적 연구- I 연어, Oncorhynchus keta의 난 발생 및 자치어의 형태)

  • Myoung, Jung-Goo;Kim, Yong-Uk
    • Korean Journal of Ichthyology
    • /
    • v.5 no.1
    • /
    • pp.53-67
    • /
    • 1993
  • In December of 1989 and 1990, matured adults of chum salmon were collected from Namdae-chun River in Yangyang-gun, Kangwon-do, Korea. Artificial insemination was made at captured locations. The fertilized eggs were hatched in trough incubators and the larvae were reared in laboratories of the Yangyang Fisheries Institute and the Korea Ocean Research and Development Institute. The fertilized eggs of this species were demersal and separated, and red in color with mean diameter of 7.2mm. The hatching took place from 480 to 531 degree($^{\circ}C$) days after fertilization. The newly hatched alevins were 1.80 to 2.56cm in total length with big yolk and lied on the bottom. In 35 days after hatching, the alevin attained $3.56{\pm}0.12cm$ in total length, and absorbed the yolk completely to become fry with 7 to 11 parr marks on the body. In 3 months after hatching, and the fries became smolt with silvery scales haring 5.67-6.53cm in total length. The early larval developments of chum salmon could be divided into three stages according to the changes in body shapes and length : larval stage before swim up, swim up stage and smolt stage. The growth of snout, trunk and body height to total length were faster than other body parts in swim up stage.

  • PDF

Studies on the Morphological Characteristics and Life History of the Chili Fish, Cultriculus eigenmanni (Jordan et Metz) (1) On the Egg Development and the Fry in the Larval Stage (치리(Cultriculus eigenmanni)의 형태 및 생활사에 관한 연구 I. 난발생과 부화자어에 대하여)

  • KIM Eul-Bae;KIM Young-Gill
    • Korean Journal of Fisheries and Aquatic Sciences
    • /
    • v.17 no.2
    • /
    • pp.151-156
    • /
    • 1984
  • The Chili fish, one of the most common species of the freshwater fishes, is distributed in the river basins of the western coast of Korea. However, any studies on this fish, especially its development or life history, have not been made so far. The authors, therefore, carried out an experiment to reveal some characteristics of egg develoment and fry in this fish. For accomplshing this study, we caught some adult Chili fish by means of a casting net at raceway in the vicinity of Kunsan in June and July, 1983; then, we injected 0. 1 cc Gonadotrophic hormone 1,000 unit solution (Puberogen) in order to induce the spawning on the abdominal cavity of male and female respectively, Approximately 12 hours after injection, the artificially fertilized eggs by using of the dry method and the naturally fertilized eggs were observed simultaneously in the aquarium ($30{\times}50{\times}70cm$). The fertilized eggs, in spherical shape, were sperative, demersel, adhesive nature and $1.38{\sim}1.59\;mm$ in diameter. The light yellow yolk was opaque and $1.17{\sim}l.27\;mm$ in diameter. The hatching of eggs took place at 29.5 hours after fertilization under the condition of $23.5{\sim}25.0^{\circ}C$ water temperature. Basically there were not found so many different aspects in comparison with the common species of Cyprinidae in the development process of eggs : however, there were a few noticable characteristics, that is, pigment cells, eye balls and blood circulation are invisible, or the velocity of egg development is rapid, seemed similar to the Loach's egg. Newly hatched larvae with the yolk sac (2.39 mm in size) were $3.87{\sim}3.99\;mm$ in total length and their myotome number was expressed as 28+15=43. About 20 hours after hatching, a caudal and a pectoral fin as well as an anus began to orgin. In addition, a few malanophore showed on the upper regin of the yolk sac of the larvae whose size was 4.73 mm in total length. The larvae developed about 5.02 mm in total length, and the median fin fold in the abdomen extended forward to two thirds or beyond of the yolk sac at 50 hours after hatching. Simultaneously, the eye balls and mouth also appeared. After 3 days, the yolk sac nearly disappeared, and the larvae (5.4 mm in total length) with perfect eye balls began to swim for themselves for the first time. Also, the specialization between the gill arches and the ailmentary canal, the development of each fins except dorsal fin as well as the blood circulation obviously distinguished in this stage. The larvae whose total length was 5.68 mm was very brisk: also, the development of the dorsal fin rudiment, nearly perfect formation of each organs and the function of the upper and lower jaws were noticeably observed 5 days after hatching.

  • PDF

Spawning Period Characteristics and Early Life History of the Eight Barbel Loach, Lefua costata (Pisces: Balitoridae) (쌀미꾸리(Lefua costata)의 산란기 특징 및 초기생활사)

  • Kim, Hyeong-Su;Han, Mee-Sook;Ko, Myeong-Hun
    • Korean Journal of Environment and Ecology
    • /
    • v.35 no.3
    • /
    • pp.285-293
    • /
    • 2021
  • This study was conducted to elucidate spawning period characteristics and early life development of eight barbel loach, Lefua costata (Balitoridae) at the Jusucheon stream, Okgye-myeon, Gangneung-si, Gangwon-do, Korea between January and December in 2018. The spawning period was estimated to be from May to August, considering the change in the gonad-somatic index, the appearance of young fry, and frequency distribution in egg diameter. It was a multi-spawning type. The gender ratio was 1:0.79 with 1,117 females and 879 males collected. The egg size was 0.24-0.93 mm, with mature and immature eggs found during the spawning period. The size of mature eggs was 0.71±0.02 mm, and the average number of fecundity was 1,786±818 (n=31). Observation of the egg development showed that the fertilized eggs were the sticky, gray, segregated, and demersal type with 0.76±0.03 mm in diameter. The hatching of the embryos began at about 34 hours (hatching rate 50%) after fertilization underwater temperature of 25℃. The average length of the newly hatched pre-larvae was 2.7±0.11 mm. The average length of pre-larvae at 4 days after hatching was 4.5±0.16 mm, and the yolk sac was completely absorbed and entered the post-larvae stage. At 20 days after hatching, the average length of post-larvae was 11.5±0.67 mm, and their fin rays were formed before they transitioned to the juvenile stage. At 100 days after hatching, the average length reached 49.8±2.60 mm, and the appearance and the lateral sideband patterns were similar to those of the adult fish.

A Survey on Food Preference of the Elementary School Students in Chollabukdo Area (전북 일부지역 초등학생의 식품 기호도에 관한 연구)

  • Chang Hye-Soon;Ahn Yeoun-Il
    • Korean Journal of Human Ecology
    • /
    • v.7 no.4
    • /
    • pp.5-23
    • /
    • 2004
  • This study was done to provide basic information for nutrition education and meal planning preference, Children's preference according to cooking method is high in fruit. drink, fried foods, dish food, but low in Sangchae, Sukchae, and Jangachies. The preference of staple food was that the most preferred foods were plain rice in rice, chicken gruel in gruels, rolled rice in one dish foods. and Jajangmyeon in noodles. The preference of side dishes was that the most preferred foods were seaweed in soups, Kimchi in Chigaes, beef and pork rib in steamed foods, beef in braised foods, Bulgogi in grilled foods, egg-fry in pan fried foods, Ddukbokki in stir-fried foods, pork cutlet in fried foods, Dotorimuk in Sangchaes, fruit in salads, bean sprouts in Sukchaes, Baechu Kimchi in Kimchies and Danmuji in Jangachies. The preference of snack was that the most preferred foods were citrus in fruits, Kkuldduk in rice cakes, hotdog in bread, ice cream in milk and dairy products and fruits juice in drink.

  • PDF