• Title/Summary/Keyword: egg albumin

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Power Absorption Measurements during NMR Experiments

  • Felix-Gonzalez, N.;Urbano-Bojorge, A.L.;de Pablo, C. Sanchez-L;Ferro-Llanos, V.;del Pozo-Guerrero, F.;Serrano-Olmedo, J.J.
    • Journal of Magnetics
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    • v.19 no.2
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    • pp.155-160
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    • 2014
  • The heating produced by the absorption of radiofrequency (RF) has been considered a secondary undesirable effect during MRI procedures. In this work, we have measured the power absorbed by distilled water, glycerol and egg-albumin during NMR and non-NMR experiments. The samples are dielectric and examples of different biological materials. The samples were irradiated using the same RF pulse sequence, whilst the magnetic field strength was the variable to be changed in the experiments. The measurements show a smooth increase of the thermal power as the magnetic field grows due to the magnetoresistive effect in the copper antenna, a coil around the probe, which is directly heating the sample. However, in the cases when the magnetic field was the adequate for the NMR to take place, some anomalies in the expected thermal powers were observed: the thermal power was higher in the cases of water and glycerol, and lower in the case of albumin. An ANOVA test demonstrated that the observed differences between the measured power and the expected power are significant.

Effects of Drinking Ion Water on Performance, Egg Quality and Blood Biochemical Composition of Laying Hens under High Temperature Conditions (고온환경에서 이온수의 급수가 산란계의 생산성, 계란 품질 및 혈액성상에 미치는 영향)

  • Lim, Chun Ik;Rana, Md Masud;Li, Hong Long;Cho, Tae Hyun;Ryu, Kyeong Seon
    • Korean Journal of Poultry Science
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    • v.47 no.3
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    • pp.121-126
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    • 2020
  • This study was conducted to investigate the effect of drinking ion water on the performance, egg quality, and serum biochemical properties of laying hens in a high-temperature environment. A total of 180 laying hens with equal egg production rates were randomly divided into three treatments with five replicates. Treatments were applied from 24 to 27 weeks of age and included the supply of ion water (IW), tap water (TW), and cold water (CW). The results showed that the egg production rate, egg weight, and feed intake of hens were significantly (P<0.01) improved in the IW group compared with those in the TW group but did not differ from those in the CW group. Egg quality parameters such as albumen height, Haugh unit, and eggshell thickness were found to be higher in the IW than in the TW group (P<0.05); however, no significant differences were observed in comparison with the CW group. Serum Ca and P concentrations tended to increase (P<0.10) in the IW group, and serum high-density lipoprotein cholesterol and albumin levels were greater (P<0.05) in response to IW than those to TW. This study provides evidence that the supply of IW can relieve the high-temperature stress during the summer. In particular, because the improved effects of the IW were confirmed to be similar to those of CW, it shows potential as a method to manage the drinking water of hens in the summer season along with the supply of CW.

Effect of γ-Aminobutyric Acid and Probiotics on the Performance, Egg Quality and Blood Parameter of Laying Hens Parent Stock in Summer (γ-Aminobutyric Acid 및 생균제 급여가 여름철 산란 종계의 생산성, 계란 품질 및 혈액 성상에 미치는 영향)

  • Ji Heon, Kim;Yoo Don, Ko;Ha Guyn, Sung
    • Korean Journal of Poultry Science
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    • v.49 no.4
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    • pp.239-246
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    • 2022
  • This study was conducted to investigate the effects of dietary γ-aminobutyric acid (GABA) and a probiotic mixture on egg production and quality, blood parameters, and stress levels (corticosterone) in Hy-Line parent stock during summer in Korea. A total of 105 Hy-Line parent stock aged 24 weeks were randomly divided into three groups, each containing thirty-five birds: control, γ-aminobutyric acid (GABA), and probiotics (1 × 108/g Bacillus licheniformis, 1 × 107/g Lactobacillus plantarum, and 1 × 107/g Corynebacterium butyricum). The hens were fed a diet containing 50 ppm GABA or 0.1% probiotics for 6 weeks. Compared with the control group, the hen-day egg production, egg mass, and feed conversion ratio over the total period were significantly higher in the probiotic group (P<0.05). In contrast no significant differences were detected among groups with respect to egg weight, albumen height, Haugh units, yolk color, shell thickness or shell strength. Similarly, no significant difference were observed among groups with regards to biochemical profile (total cholesterol, triglyceride, glucose, total protein, aspartate aminotransferase, alanine aminotransferase, albumin, and inorganic phosphorus). However, compared with the control group, we did detect significant reductions in corticosterone levels in the GABA and probiotics groups (P<0.05). On the basis of our findings in this study, it would appear that dietary GABA and probiotics can alleviate heat stress in Hy-Line parent stock, with probiotics in particular being found to promote significant improvements in the hen-day egg production, egg mass, and feed conversion of laying hens during the summer season in Korea.

Effects of Heat Treatment on the Antigenicity of Gamma-Irradiated Egg White Albumin (감마선 조사된 난백 알부민의 항원성에 미치는 가열의 영향)

  • 이주운;육홍선;조경환;김미리;김천제
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.5
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    • pp.848-853
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    • 2001
  • This research was conducted to study the effects on antigenicities (allergenicity) and structural changes of gamma-irradiated hen’s egg albumin (ovalbumin, OVA) by heating. Three groups of OVA solution (2.0 mg/mL) were prepared; 1) heat treatment; 2) irradiation after heating; 3) heating after irradiation. Samples were isothermally heated and/or irradiated at the absorption dose of 10 kGy. Competitive indirect ELISA was individually formatted with egg-allergic patients IgE (P-IgE), and mouse murine monoclonal IgG (M-IgG) and rabbit polyclonal IgG (R-IgG) for evaluating bindinhg abilities of antibodies to OVA in the sample solutions. Binding abilities of antibodies to thermally denatured OVA were changed : R-IgG to the sample treated with above 6$0^{\circ}C$, M-IgG to that above 7$0^{\circ}C$, and P-IgE to that above 8$0^{\circ}C$, respectively. P-IgE did not well recognize OVA heated at 8$0^{\circ}C$ and the above. However, binding abilities of M-IgG and R-IgG highly in creased. Significant differences of binding abilities were not observed in all samples with the combination of heat treatment and irradiation, regardless the order of the treatment. Turbidity of samples in creased both by heating and by irradiation, and the increase by irradiation was much higher than by heating. These results showed that allergenicity of OVA reduced by gamma irradiation was not affected by heating.

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Studies on the Paper Electrophoresis, I On the Mobility of Egg Albumin(Ovalbumin) (페이퍼 에레크트로포레시스에 關한 硏究 (제1보) 계란단백의 移動度에 關하여)

  • Chang, Sei-Hun;Kim, Si-Joong
    • Journal of the Korean Chemical Society
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    • v.4 no.1
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    • pp.1-6
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    • 1957
  • The mobilities and the electrophoretic phenomena of human serum albumin, and of horse serum albumin, etc., on paper were reported, but there is no information about the mobility and the electrophoretic phenomena of ovalbumin. In this study they are determined and considered at various time durations. The mean value of this mobility is compared with the value determined by the electrophoresis in solution. Also, the mobilities of the electroendosmotic flow, which affect on the mobility seriously, are determined at various time durations. Apparatus and method used, are a closed system type and a method with electrode vessels. It is concluded that: 1) The mobilities of ovalbumin have the nearly constant mobilities at six and eight hours of time durations, but it decreases at ten hours of long time duration. Their mean value is (0.243 ${\pm}$ 0.003)cm./hr./v./cm. toward anode at pH 8.0. 2) Under these experimental conditions, ovalbumin cannot be separated into three fractions (A1, A2, A3) on paper. 3) Most of the factors, which affect on the mobility, are fixed by the initial experimental conditions, but the electroendosmotic flow due to the characteristic capillarity of paper is measured. Then, the mean value of its mobilities shows the good constancy, and the value is (0.073 ${\pm}$ 0.0003)cm/hr./v./cm. toward cathode at pH 8.0. 4) By the above facts, if the same paper and the same experimental conditions are chosen, it may be preferred to determine the mobility of the electroendosmotic flow once time, even when many observations are required.

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Effects of Corn Distiller's Dried Grains with Solubles on Production and Egg Quality in Laying Hens

  • Cheon, Y.J.;Lee, H.L.;Shin, M.H.;Jang, A.;Lee, S.K.;Lee, J.H.;Lee, B.D.;Son, C.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.21 no.9
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    • pp.1318-1323
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    • 2008
  • Corn distiller's dried grains with solubles (DDGS) is a completely new feed ingredient in the Korean feed market. There is an ever increasing need for the Korean feed industry to import and make the best of it as a high protein and high energy feed ingredient. A layer feeding trial was conducted for 10 weeks to investigate the effects of addition of light-colored DDGS to layer diets on laying performance, egg qualities and yolk fatty acid composition. Also, the economics of using DDGS in the Korean situation was analyzed. Nine hundred Hy-line Brown layers, 24 weeks of age, were employed in a feeding trial consisting of four dietary treatments (0, 10, 15, and 20% DDGS), and five replicates per treatment. All experimental diets were prepared as iso-protein (17%) and iso-calorie (TMEn 2,780 kcal/kg). The use of DDGS up to 20% in layer diets did not exert any influence on feed intake, laying rate, total egg mass, mean egg weight and feed conversion ratio (p>0.05). The color and breaking strength of eggshell, as well as the albumin height and Haugh unit were not affected by the addition of DDGS up to 20% in the diet. The yolk color was significantly increased by DDGS supplementation (p<0.05). As the DDGS level increased, the oleic acid content decreased, and the linoleic acid increased (p<0.05) in egg yolk. The degree of saturation of yolk fatty acids was not affected by DDGS supplementation. The inclusion of light-colored DDGS up to 20% in layer diets resulted in a decrease of feed cost per kg without any undesirable effect on laying performance. In conclusion, the light-colored DDGS (L* 56.65) could be used up to 20% in layer diets without any harmful effect on laying performance, and possibly provide economic benefits to the Korean poultry industry.

Effects of Three Different Soybean Meal Sources on Layer and Broiler Performance

  • Park, Y.H.;Kim, H.K.;Kim, H.S.;Lee, H.S.;Shin, I.S.;Whang, K.Y.
    • Asian-Australasian Journal of Animal Sciences
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    • v.15 no.2
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    • pp.254-265
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    • 2002
  • Soybean meal (SBM) is a major protein source in poultry feeds and one of the best quality ingredients because of the relatively high protein content, good amino acid profile and bioavailability. But soybean meal quality is largely dependent on the processing technology and origins. In this experiment, effects of three different soybean meals were evaluated in layer (experiment 1) and broiler (experiment 2). Soybean meal sources used in the experiments were the US-originated dehulled soybean meal (USDHSBM), India-originated non-dehulled soybean meal (India SBM) and Brazil-originated non-dehulled soybean meal (Brazil SBM). Experiment 1 was conducted during growing and laying periods and evaluated the interactive effects of soybean meal sources according to feeding periods on growth performance and egg quality. Experiment 2 was conducted during growing period (day 1-35) and finishing period (day 35-42). The growth performance was measured for the same periods and any possible interaction between soybean meal origins and crude protein levels was also studied. In experiment 1, chicks fed India SBM utilized feed more efficiently (p<0.05) than those fed Brazil SBM from day 29 to day 42. The body weights of layers during the laying period had no relation to egg production. But egg weights were significantly heavier in all the USDHSBM fed groups than other groups (p<0.001) and depended on feed protein source during growing period (p<0.001). The average egg weight of the USDHSBM fed group scored the highest value (65.4 g), followed by the Brazil SBM fed group (62.1 g) and India SBM fed group (62.1 g). There was an effect of interaction between origins of soybean meal fed group in growing and laying period on eggshell color (p<0.01). Eggshell was significantly stronger in the USDHSBM fed (for growing period) groups than other groups (p<0.05) on $31^{st}$ week. Haugh's unit (HU), albumin index and yolk index of the USDHSBM fed group in growing stage were significantly superior (p<0.001) to other groups. In experiment 2, for the 7-week, chicks on the India SBM group gained less (p<0.001) weight than other groups. While daily gain of India SBM chicks was not affected by dietary crude protein level, those of the USDHSBM and Brazil SBM chicks were linearly increased as dietary crude protein level increased from 18% to 20%. The gain per feed ratio of the USDHSBM group was the highest (0.585), followed by the Brazil SBM group (0.568) and India SBM group (0.550) (p<0.01). Therefore, in this experiment, the use of USDHSBM with excellent protein quality and amino acid digestibility could be of advantage to the economic production of layer and broiler.

Effect of Feeding Rare Earth on Egg Production and Hatchability Broiler Growth (희토(稀土)의 급여가 종계의 산란율 및 부화율에 미치는 영향)

  • Ham, S.K.;Song, T.H.;Zhang, G.Q.;Hur, S.N.;Park, H.S.
    • Korean Journal of Poultry Science
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    • v.33 no.3
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    • pp.225-231
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    • 2006
  • To investigate the effect of feeding rare earth (RE) on the performance of breeder hens a feeding trial with sixteen thousands of 158 day old Ross broiler breeder hens was conducted for thirty weeks. A mixture of RE- chlorides containing mainly La, Ce, and Pr was mixed into corn-soy based diet at two levels, 300 and 600 mg/kg, All the hens were housed in flat layer houses and the ratio of male to female was maintained at one to ten. Dietary supplementation of the of RE at a level of 300 mg/kg made the hens reach egg production peak higher by about 6% and earlier by about two weeks. As a result, it made higher hatchable egg production by 3.5%. It also reduced dramatically the mortality of both male and female breeders(P<0.05). Egg weight was slightly increased but egg qualify was not much influenced by dietary supplementation of RE. Egg albumin hight and Haugh unit were significantly improved while egg shell thickness, egg breaking strength and yolk color were similar to those of the control. Experimental results appeared to show a good possibility that egg fertility and hatchability were improved by feeding RE. Dietary supplementation of RE at a level of 300 mg/kg should be acceptable but not the 600 mg/kg level for breeder hens. Further studies on the effect of RE on egg fertility and hatchability appears to be necessary.

Effect of Feeding Thymus vulgaris Powder on the Productivity, Egg Quality and Egg Yolk Fatty Acid Composition in Laying Hens (Thymus vulgaris 분말의 급여가 산란계의 생산능력, 난품질 및 난황 내 지방산 조성에 미치는 영향)

  • Park, Seong-Bok;Lee, Kyeong-Jin;Lee, Wang-Hyu;Ryu, Kyeong-Seon
    • Korean Journal of Poultry Science
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    • v.39 no.2
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    • pp.157-161
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    • 2012
  • The objective of this study was to evaluate the effects of feeding dried Thymus vulgaris powder (TVP) on the layer performance, egg quality and egg yolk fatty acid composition. Two hundred eighty eight, 35 weeks old Brown nicks were allocated in the cage for five TVP (0, 0.25, 0.5, 1.0 and 2.0%) diets with four replicates in each treatment. Each basal diet contained 16.0% CP and 2,800 kcal/kg ME and supplied to layers for 8 weeks. Egg production and egg weight were not statistically different but numerically increased in birds fed 2.0% TVP than other treatments. Egg mass was not consistent depending on the TVP addition levels in diets. Feed intake was significantly lower in TVP addition treatments than the control (p<0.05), however, feed conversion was not significantly differed among control and treatments (p<0.05). Dietary addition of 2.0% TVP showed high values on albumin height, Haugh unit and egg yolk color and they are 7.93, 86.79 and 7.20, respectively (p<0.05). Palmitic and arachidonic acids were remarkably lowered when 2.0% TVP added to basal diets and then fed to birds for 8 weeks, in contrast, significantly higher linolenic acid composition was determined in birds fed 2.0% TVP (p<0.05). As the results, the highest unsaturated fatty composition and UFA/SFA ratio were observed in 2.0% TVP treatment as compared to those of control and other treatments. Therefore, the results indicate that the Thymus vulgaris powder addition to basal diet tended to have positive effects on egg quality and egg yolk fatty acids composition, and the optimum addition of Thymus vulgaris powder may be 2.0%.

Genetic Relationships of Korean Ocenebrine Species (Gastropoda: Prosobranchia: Muricidae)

  • Park, Joong-ki;Choe, Byung-Lae
    • Animal cells and systems
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    • v.3 no.3
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    • pp.285-293
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    • 1999
  • Allele frequencies in twenty-two populations from nine Korean muricid species of five genera (Rapaninae and Ocenebrinae) wire analyzed genetically using 13 loci from 11 allozyme systems. The clustering patterns of these species were basically consistent with traditional groupings to two subfamilial categories based on shell and radula morphologies. Within six ocenebrine species it was apparent that Ceratostoma inornata and Pteropurpura adunca were most closely related to each other than to any other species belonging to the genus Ceratostoma. These results, along with other anatomical features 〔morphologies of albumin gland (female), egg capsule〕, suggest that the generic position of C. inornata, previously classified to be congeneric with other western Pacific Ceratostoma species, should be reconsidered. Our results show the close relationship of Nucella freycineti with other ocenebrine species, rather than with rapanines, which supports a previous suggestion that the genus Nucella should be placed with the subfamily Ocenebrinae rather than the subfamily Rapaninae.

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