• 농촌지도와개발
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• 제16권1호
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• pp.125-152
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• 2009

The objective of this study, which applies the multiple-disciplinary approach to the developmental characteristics of children, is to study and develop a class model that can be applied to actual kindergarten classes in rural area. For this purpose, this study proposes teaching and learning methodologies for children based on English children's tales to help make the English education of children more effective and efficient. Based on the findings, the following suggestions should be considered for improving the English-education class model for kindergartners for rural areas: First, various activities based on the multiple-intelligence approach are important methods of children-oriented education advanced by the Sixth Children's Curriculum, helping children grow their independence and creativity. Second, various activities developed by this study on the basis of the multiple-intelligence approach to promote children's reading, listening, speaking, and writing abilities helped children improve their linguistic capacities, improve creativity, and remain motivated, which was reinforced by the differences found between the test group and the control group.

• The Plant Pathology Journal
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• 제16권1호
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• pp.43-47
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• 2000

Citrus tristeza virus (CTV) is the causal agent of one of the most important diseases of citrus. Recently, CTV has been detected in Cheju Island by ELISA. The coat protein (CP) gene of CTV isolated form Cheju Island was cloned by RT-PCR and the nucleotide was analyzed in this study. Citrus leaves were collected from trees showing decline symptoms from various region of Cheju Island in the summer of 1998 and 1999. The CP gene open reading frame is composed of 670 nucleotides and encodes a polypeptide of 223 amono acids. Sequence analysis the CP gene revealed that two CTV strains present in Cheju Island. Viruses collected form Sogwipo area and Cheju City area in 1999 ahowed 91-93% nucleotide sequence homology with CTV T36 strain. Viruses collected form Cheju City area in 1999 and Sogwipo City in 1998 showed 94-98% nucleotide sequence homology with CTV SY568 strain. A efficient viral RNA extraction methods was developed by modifying procedure for animal virus RNA purification methods and PCR product was detected form one tenth of RNA purified from as small as 45 mg fresh or frozen tissue.

• 한국멀티미디어학회논문지
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• 제16권5호
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• pp.566-576
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• 2013

In Home Area Network (HAN) of the smart grid, Power Line Communication (PLC) technology and ZigBee communication technology can be used in the communication among the Advanced Metering Infrastructure (AMI) devices. However, according to performance evaluation results of the PLC technology, we find that using the PLC technology is unsuitable for the remote meter reading service. It is worth noting that some parts of the PLC are converted to the ZigBee communication technology in Jeju, Korea. Compared with PLC, ZigBee has no restriction of the place, where the equipments can be freely set up, due to the advantage of radio communication. However, number of usable devices will impact the network performance which is depended on the address assignment. In addition, due to the restriction of transmission range among devices, it is difficult to apply the ZigBee address assignment method to the practical circumstance. In this paper, we examine the previous ZigBee address assignment schemes and the corresponding routing algorithms, and propose a novel address assignment scheme compared with the existing methods, the performance of the proposed one is improved. In particular, evaluation results show that the proposed scheme reduces the average number of hop count, the transfer time and the processing time.

• Journal of Biosystems Engineering
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• 제35권2호
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• pp.132-137
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• 2010

Site-specific N application for corn is one of the precision crop management. To implement the site-specific N application, various nitrogen stress sensing methods, including aerial image, tissue analysis, soil sampling analysis, and SPAD meter readings, have been used. Use of side-dressing, an efficient nitrogen application method than a uniform application in either late fall or early spring, relies mainly on the capability of nitrogen deficiency detection. This paper presents map-based variable rate nitrogen application based using a multi-spectral corn nitrogen deficiency(CND) sensor. This sensor assess the nitrogen stress by means of the estimated SPAD reading calculated from the corn leave reflectance. The estimated SPAD value from the CND sensor system and location information form DGPS of each field block was combined into the field map using a ArcView program. Then this map was converted into a raster file for a map-based variable rate application software. The relative SPAD (RSPAD = SPAD over reference SPAD) was investigated 2 weeks after the treatments. The results showed that the map-based variable rate application system was feasible.

• 대한수의학회지
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• 제39권4호
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• pp.772-777
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• 1999

The uncaped genomic RNA of bovine viral diarrhea virus (BVDV) initiates translation by recruitment of eukaryotic translation initiation factors at the internal ribosome entry site (IRES). N-terminal protease ($N^{pro}$) is the first translation product of the open reading frame (ORF). By using the vaccinia virus SP6 RNA polymerase transient expression system, we showed previously that deletion of $N^{pro}$ region reduced translation by 21%. To better understand the biological significance of $N^{pro}$ for translation, we carried out a mutational analysis of the $N^{pro}$ region of BVDV cloned in the intercistronic region of a bicistronic reporter plasmid. We constructed a bicistronic expression vector in which the entire 5 UTR and the mutated $N^{pro}$ region (${\Delta}386-901$, ${\Delta}415-901$ and ${\Delta}657-901$) was cloned between two reporter genes, chloramphenicol acetyltransferase (CAT) and luciferase (LUC). In vivo translation analyses showed that $N^{pro}$ region was dispensible for efficient translation. The results indicate that the $N^{pro}$ region is not essential for BVDV RNA translation and the 3' boundary of BVDV IRES is expanded into $N^{pro}$ region, suggesting that $N^{pro}$ may not play a major role in BVDV replication.

• Journal of Communications and Networks
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• 제14권6호
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• pp.619-628
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• 2012

The ZigBee network has been considered to monitor electricity usage of home appliances in the smart grid network. ZigBee, however, may suffer from a coexistence problem with wireless local area network (WLAN). In this paper, to resolve the coexistence problem between ZigBee network and WLAN, we propose a new protocol constructing a cognitive smart grid network for supporting monitoring of home appliances. In the proposed protocol, home appliances first estimates the transmission timing and channel information of WLAN by reading request to send/clear to send (RTS/CTS) frames of WLAN. Next, based on the estimated information, home appliances transmit a data at the same time as WLAN transmission. To manage the interference between WLAN and smart grid network, we propose a cognitive beamforming algorithm. The beamforming algorithm is designed to guaranteeing zero interference to WLAN while satisfying a required rate for smart metering. We also propose an energy efficient rate adaptation algorithm. By slowing down the transmission rate while satisfying an imperceptible impact of quality of service (QoS) of the receiver, the home appliance can significantly save transmit power. Numerical results show that the proposed multiple antenna technique provides reliable communications for smart metering with reduced power comparing to the simple transmission technique.

• Journal of Microbiology and Biotechnology
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• 제2권1호
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• pp.56-65
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• 1992

The complete nucleotide sequence of the Bacillus circulans F-2 RSDA gene, coding for raw starch digesting a-amylase (RSDA), has been determined. The RSDA structure gene consists of an open reading frame of 2508 bp. Six bp upstream of the translational start codon of the RSDA is a typical gram-positive Shine-Dalgarno sequence and the RSDA encodes a preprotein of 836 amino acids with an Mr of 96, 727. The gene was expressed from its own regulatory region in E. coli and two putative consensus promoter sequences were identified upstream of a ribosome binding site and an ATG start codon. Confirmation of the nucleotide sequence was obtained and the signal peptide cleavage site was identified by comparing the predicted amino acid sequence with that derived by N-terminal analysis of the purified RSDA. The deduced N-terminal region of the RSDA conforms to the general pattern for the signal peptides of secreted prokaryotic proteins. The complete amino acid sequence was deduced and homology with other enzymes was compared. The results suggested that the Thr-Ser-rich hinge region and the non-catalytic domain are necessary for efficient adsorption onto raw substrates, and the catalytic domain (60 kDa) is necessary for the hydrolysis of substrates, as suggested in previous studies (8, 9).

• 한국통신학회논문지
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• 제36권7B호
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• pp.848-857
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• 2011

무선통신 기반의 원격검침 시스템은 설치의 용이함, 설비공사 및 유지보수 비용절감, 다양한 검침시스템으로의 적용의 유연성, 그리고 네트워크 확장성의 용이함 등의 장점으로 인해 최근 다양한 원격검침분야에서 활발히 활용되고 있다. 하지만, 이같은 다수의 장점에도 불구하고, 멀티 홉 기반의 원격검침망에서는 매 검침데이타 획득시마다 과다한 양의 트래픽을 발생시킴으로써, 데이터 충돌과 간섭의 확률을 증대시키고, 불필요한 전송으로 인한 에너지 낭비와 함께 특정 노드들의 에너지만 급격하게 소모되는 에너지 불균형 현상을 야기하고 있다. 그리하여 본 논문에서는 멀티홉 기반의 무선 원격검침망에서 발생하는 트래픽의 양을 최소로 줄임으로써 검침망의 각 노드들의 에너지 보존은 물론 다양한 문제점들을 해결할 수 있는 효율적인 검침데이타 수집기법을 제안하고, 성능평가를 통해 제안하는 기법에 대한 성능의 우수성을 입증한다.

• Journal of Microbiology and Biotechnology
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• 제31권9호
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• pp.1323-1329
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• 2021

Micro-scale magnetic beads are widely used for isolation of proteins, DNA, and cells, leading to the development of in vitro diagnostics. Efficient isolation of target biomolecules is one of the keys to developing a simple and rapid point-of-care diagnostic. A zinc finger protein (ZFP) is a double-stranded (ds) DNA-binding domain, providing a useful scaffold for direct reading of the sequence information. Here, we utilized two engineered ZFPs (Stx2-268 and SEB-435) to detect the Shiga toxin (stx2) gene and the staphylococcal enterotoxin B (seb) gene present in foodborne pathogens, Escherichia coli O157 and Staphylococcus aureus, respectively. Engineered ZFPs are immobilized on a paramagnetic bead as a detection platform to efficiently isolate the target dsDNA-ZFP bound complex. The small paramagnetic beads provide a high surface area to volume ratio, allowing more ZFPs to be immobilized on the beads, which leads to increased target DNA detection. The fluorescence signal was measured upon ZFP binding to fluorophore-labeled target dsDNA. In this study, our system provided a detection limit of ≤ 60 fmol and demonstrated high specificity with multiplexing capability, suggesting a potential for development into a simple and reliable diagnostic for detecting multiple pathogens without target amplification.

• 한국육종학회지
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• 제42권5호
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• pp.455-462
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• 2010

Purple acid phosphatase is important for phosphorus remobilization in plants, but its role in plant adaptation to low phosphorus availability is not known. The cDNA encoding O. sativa purple acid phosphatase (OsPAP1) has 1008 bp with an open reading frame of 335 amino acid residues. The amino acid sequence of OsPAP1 cDNA shows of 50-51% identity with other plant purple acid phosphatases. OsPAP1 was expressed in rice plants and in cell cultures in the absence of phosphate ($P_i$). The expression was organ-specific with the strongest expression in $P_i$-deprived roots. Functional expression of the OsPAP1 gene in the transgenic Arabidopsis line was confirmed by northern and western blot analysis. OsPAP1 overexpression lines had higher phosphatase activity than wild-type. Overexpression of OsPAP1 in Arabidopsis plants resulted in increased Pi accumulation under Pi sufficient condition. These results show that the OsPAP1 gene represents more efficient $P_i$ uptake and can be used to develop new transgenic dicotyledonous plants.