• Genomics & Informatics
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• 제6권1호
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• pp.36-43
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• 2008

Radiotherapy would be the choice of treatment for human cancers, because of high cost-effectiveness. However, a certain population of patients shows a resistance to radiotherapy and recurrence. In an effort to increase the efficacy of radiotherapy, many efforts were driven to find the genes causing the unresponsiveness to ionizing radiation. In this paper, we compared the gene expression profiles of two lung cancer cell lines, H460 and H1299, which showed differential responses to ionizing radiations. Each cell were irradiated at 2 Gy, and harvested after 0, 2, 4, 8, 12 and 24 hours to examine the expressions. Two-way ANOVA analysis on time-series experiments of two cells could select 2863 genes differentially expressed upon ionizing radiation among 32,321 genes in microarray (p<0.05). We classified these genes into 21 clusters by SOM clustering according to the interaction between cell types and time. Two SOM clusters were enriched with apoptosis-related genes in pathway analysis. One cluster contained higher levels of phosphatidyl inositol 3-phosphate kinase (PI3K) subunits in H1299, radio-resistant cells than H460, radiosensitive cells. TRAIL receptors were expressed in H460 cells while the decoy receptor for TRAIL was expressed in H1299 cells. From these results, we could characterize the differential responsiveness to ionizing radiation according to their differential expressions of apoptosis-related genes, which might be the candidates to increase the power of radiotherapy.

• 한국수정란이식학회지
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• 제25권2호
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• pp.97-101
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• 2010

Intracytoplasmic sperm injection (ICSI) is one of the artificial fertilization methods when only a few sperm are available for insemination, and an important tool for the preservation of genetic materials of endangered animal species, especially the male is infertile. Different from other species such as mice and pigs, the conventional ICSI method which uses spiked pipette for injection (Spike-ICSI) is exhibited low success rates in cattle because the bovinesperm head membrane is hard to break during injection procedure. We chose piezo-assisted ICSI (Piezo-ICSI) for the improvement of the injection procedure including sperm head membrane rupture and efficient puncture of the plasma membrane of the oocytes. In this experiment, we compared the efficacy of the bovine ICSI embryo production between the Piezo-ICSI and Spike-ICSI. The second polar body extrusion, pronuclear formation, cleavage and blastocyst formation were evaluated after implementation of two different ICSI techniques. The Piezo-ICSI tended to show comparably higher rates of the second polar body extrusion (41.7%), the pronuclei formation (42.9%) and the two-cell cleavage (41.4%) than Spike-ICSI does (33.3%, 28.6% and 23.5%, respectively) although there is no statistic significance between two groups. In addition, the blastocysts were only obtained from the Piezo-ICSI group (10.3%). Our finding shows that the Piezo-ICSI may be used as an artificial fertilization method in cattle when in vitro fertilization is not applicable.

• 대한의용생체공학회:의공학회지
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• 제36권6호
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• pp.291-295
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• 2015

Various types of implantable drug delivery devices have attracted significant attention for several decades to improve drug bioavailability and reduce side effects, thus enhancing therapeutic efficacy and patients' compliance. However, when implanted into the body, the devices may be influenced by the changes in physiological condition, such as temperature, pH or ionic concentration. Thus, the drug release rates could be also altered concurrently. Therefore, in this work, we employed an implantable ALZET$^{(R)}$ Osmotic Pump, which has been widely used to locally deliver various therapeutic agents and examined the effect of pH, temperature and ionic concentration on its drug release rate. For this, we performed in vitro cell tests to simulate the condition of local tissues influenced by the altered drug release rates, where we used diclofenac sodium as a model drug.

• Biomolecules & Therapeutics
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• 제17권2호
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• pp.138-143
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• 2009

We have reported previously on a replication incompetent recombinant adenoviral vector, AdLPCD, in which the expression of cytosine deaminase gene (CD) is driven by the tumor-specific L-plastin promoter. AdLPCD vector had been evaluated for its efficacy of chemosensitization of ovarian cancer cells to 5-FC. In spite of the fact that ovarian cancer cells, i.e., OVCAR-3 and SK-OV-3, are capable for adenoviral transduction judged by LacZ reporter gene analysis, two cell lines demonstrated quite different sensitivities toward AdLPCD/5-FC system. In OVCAR-3 cells, infection of AdLPCD followed by exposure to 5-FC resulted in the suppression of cell growth with statistical significance. On the other hand, SK-OV-3 cells were more resistant to the CD/5-FC strategy compared with OVCAR-3 cells under the same condition. The object of study was to investigate factors that would determine the sensitivity to AdLPCD/5-FC. We evaluated conversion rate of 5-FC to 5-FU after infection of AdLPCD by HPLC analysis, $IC_{50}$ of 5-FU, the expression level of integrin receptors i.e., ${\alpha}v{\beta}3$ and ${\alpha}v{\beta}5$, and status of p53 in OVCAR-3 and SK-OV-3 cells. The results indicated that OVCAR-3 cells have few favorable features compared with SK-OV-3 cells to be more effective to the AdLPCD/5-FC strategy; higher level of ${\alpha}v{\beta}5$ integrin, higher rate of conversion of 5-FC into 5-FC, and lower $IC_{50}$ of 5-FU. The results suggest that the replacement of 5-FU with CD/5-FC in combination chemotherapy would be less toxic and much greater cytotoxicity than the conventional combination chemotherapy in some patients.

• 방사선산업학회지
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• 제11권1호
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• pp.7-11
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• 2017

RI-Biomics는 방사성동위원소를 이용한 신약 및 물질의 흡수, 분포, 대사 및 배설(RI-ADME)과 같은 생체 내 역학을 평가하기 위한 최첨단 방사선 융합 기술이다. 현재 RI-Biomics 기술정보는 RI-Biomics 기술정보시스템인 'RIBio-Info'로 제공되고 있으며, 본 연구에서는 최근 빅 데이터 기술 동향을 검토하여 RIBio-Info의 개선사항을 도출하였다. RI Biomics 정보 시스템에서 빅데이터를 적용하기 위해 실현되어야 할 구성 요소로 자원, 기술 및 인력에 대해 각각 검토하였다. 첫째 활용할 수 있는 외부 빅데이터를 찾고, 두번째로 빅데이터 활용 실현을 위한 인프라를 확충하고, 마지막으로 대용량 정보분석이 가능한 데이터 사이언티스트를 양성해야 한다. 이러한 환경 하에 비정형 대용량 데이터에 대한 다양한 분석이 가능한 원천기술이 개발될 것이며, RI-Biomics 분야의 새로운 가치를 창출할 수 있는 기반이 구축될 것이다.

• Journal of Trauma and Injury
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• 제36권3호
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• pp.231-241
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• 2023

Purpose: Overtriage and undertriage rates are critical metrics in trauma, influenced by both trauma team activation (TTA) criteria and compliance with these criteria. Analysis of undertriaged patients at a level I trauma center revealed suboptimal compliance with existing criteria. This study assessed triage patterns after implementing compliance-focused process interventions. Methods: A physician-driven, free-text alert system was modified to a nonphysician, hospital dispatcher-guided system. The latter employed dropdown menus to maximize compliance with criteria. The preintervention period included patients who presented between May 12, 2020, and December 31, 2020. The postintervention period incorporated patients who presented from May 12, 2021, through December 31, 2021. We evaluated appropriate triage, overtriage, and undertriage using the Standardized Trauma Assessment Tool. Statistical analyses were conducted with an α level of 0.05. Results: The new system was associated with improved compliance with existing TTA criteria (from 70.3% to 79.3%, P=0.023) and decreased undertriage (from 6.0% to 3.2%, P=0.002) at the expense of increasing overtriage (from 46.6% to 57.4%, P<0.001), ultimately decreasing the appropriate triage rate (from 78.4% to 74.6%, P=0.007). Conclusions: This study assessed a workflow change designed to improve compliance with TTA criteria. Improved compliance decreased undertriage to below the target threshold of 5%, albeit at the expense of increased overtriage. The decrease in appropriate triage despite compliance improvements suggests that the current criteria at this institution are not adequately tailored to optimally balance the minimization of undertriage and overtriage. This finding underscores the importance of improved compliance in evaluating the efficacy of TTA criteria.

• 산업융합연구
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• 제22권5호
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• pp.117-125
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• 2024

자연 재해와 대규모 사회 재난발생으로 인해 체계적이고 전문적인 재난교육의 필요성이 증가하고 있다. 본 연구는 간호학생과 간호사를 대상으로 한 재난교육에서 교육적 개입의 효과를 종합적으로 분석한 체계적 문헌고찰 연구이다. 주요 한국 데이터베이스에서 재난, 교육 프로그램, 시뮬레이션 관련 키워드로 문헌을 검색하였으며, 재난교육 프로그램 중재를 제공한 국내 간호사 및 간호대학생을 대상으로 한 논문들이 분석 대상이었다. 대부분의 프로그램에서 이론 강의, 시뮬레이션, 디브리핑 방법이 사용되었으며, 중재 시간은 2시간 이하에서 40~56시간에 이르는 다양한 구성으로 진행되었다. 가장 많이 측정한 변수는 재난간호 준비도와 재난간호수행능력이었으며, 재난교육 프로그램의 효과로 재난간호 준비도, 재난간호수행능력, 재난태도, 재난지식, 재난인식, 학습 자기효능감, 실습만족도, 재난간호 자신감 변수에 긍정적인 영향을 미친 것으로 나타났다. 다양한 중재 시간과 방법을 고려하여 참여자들에게 실제적이고 현장감 있는 학습 경험을 제공할 수 있는 프로그램을 구성하고 효과를 검증하는 것이 중요하다.

• Asian Pacific Journal of Cancer Prevention
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• 제13권8호
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• pp.4031-4036
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• 2012

Background: The negative signaling provided by interactions of the co-inhibitory molecule, programmed death-1 (PD-1), and its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2), is a critical mechanism contributing to tumor evasion; blockade of this pathway has been proven to enhance cytotoxic activity and mediate antitumor therapy. Here we evaluated the anti-tumor efficacy of AAV-mediated delivery of the extracellular domain of murine PD-1 (sPD-1) to a tumor site. Material and Methods: An rAAV vector was constructed in which the expression of sPD-1, a known negative regulator of TCR signals, is driven by human cytomegalovirus immediate early promoter (CMV-P), using a triple plasmid transfection system. Tumor-bearing mice were then treated with the AAV/sPD1 construct and expression of sPD-1 in tumor tissues was determined by semi quantitative RT-PCR, and tumor weights and cytotoxic activity of splenocytes were measured. Results: Analysis of tumor homogenates revealed sPD-1 mRNA to be significantly overexpressed in rAAV/sPD-1 treated mice as compared with control levels. Its use for local gene therapy at the inoculation site of H22 hepatoma cells could inhibit tumor growth, also enhancing lysis of tumor cells by lymphocytes stimulated specifically with an antigen. In addition, PD-1 was also found expressed on the surfaces of activated CD8+ T cells. Conclusion: This study confirmed that expression of the soluble extracellular domain of PD-1 molecule could reduce tumor microenvironment inhibitory effects on T cells and enhance cytotoxicity. This suggests that it might be a potential target for development of therapies to augment T-cell responses in patients with malignancies.

• 한국산학기술학회논문지
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• 제16권11호
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• pp.7575-7581
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• 2015

corticotropin releasing factor(CRF)는 스트레스에 의해 유도되는 신경펩타이드 물질들 중 하나로서 모발의 손실 및 재성장에 영향을 미친다고 광범위하게 제기되어 왔다. 이에 CRF1 수용체 길항제 개발을 위하여 세포 내 칼슘 신호전달 기전을 이용한 스크리닝 시스템을 개발하고 최적화 연구를 수행하고자 하였다. 이를 위하여 에쿼린 모체세포에 CRF1 수용체와 만능 G 단백질인 G${\alpha}$16 유전자를 동시에 발현시켜 안정화 세포주를 구축하였다(HEK293a16/hCRF1). 표준 효현제인 sauvagine의 반응이 임시 발현세포와 비교하여 안정화 세포주에서 농도 의존적 반응 범위가 12배 이상 증가하였으며($EC_{50}:15.21{\pm}1.83nM$), 이에 따라 길항제 스크리닝에 필수적인 안정적인 신호와 높은 용매 허용도를 확보할 수 있었다. CRF1 수용체에 대한 표준 길항제인 antalarmin과 CP154526에 대한 $IC_{50}$ 수치는 각각 $414.1{\pm}5.5$와 $290.7{\pm}1.9nM$로 확인되었는데 냉동보관세포의 경우에도 유사한 결과를 얻었다. 에쿼린 기반 세포 기능실험의 최적화 연구를 통해 구축된 CRF 수용체 안정화 세포주는 모발의 재형성과 관련된 신규의 기능성 화장품 및 조절물질 개발 연구에 적극적으로 활용 가능 할 것으로 판단된다.

• 한국수자원학회논문집
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• 제57권3호
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• pp.209-223
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• 2024

4차 산업혁명 시대에 접어들어 데이터 기반의 의사결정이 보편화되고 있다. 하지만 데이터 품질이 확보되지 않은 채 수행되는 데이터 분석은 왜곡된 결과를 낳을 가능성이 존재한다. 수자원 관리의 기초가 되는 수위 데이터도 마찬가지로 결측, 스파이크, 잡음 등 다양한 품질 문제를 가진다. 본 연구에서는 잡음으로 인해 발생하는 데이터 품질 문제를 해결하고자 하였다. 잡음은 데이터의 트렌드 분석을 어렵게 하고 비정상적인 이상치를 생성할 가능성이 있다. 본 연구는 이러한 문제를 해결하기 위해 Wavelet Transform을 이용한 잡음 제거 접근 방안을 제안한다. Wavelet Transform은 신호처리에 주로 사용되는 방법으로 잡음 제거에 효과적인 것으로 알려져 있으며 수집된 데이터의 정답 데이터(True value) 수집을 요구하지 않으므로 시간과 비용을 줄일 수 있다는 점에서 적용이 용이한 편이다. 본 연구는 Wavelet Transform의 성능 평가를 위해 대표적인 머신러닝 기반 잡음 제거 방법인 Denoising Autoencoder와 성능 비교를 수행하였다. 그 결과 Wavelet Transform 중 Coiflets 함수는, Denoising Autoencoder에 비해 Mean Absolute Error, Mean Absolute Percentage Error, Mean Squared Error 등 모든 측면에서 우수한 성능을 보이는 것으로 나타났다. 이러한 결과는 환경에 맞는 적절한 웨이블릿 함수의 선택을 통한 잡음 문제를 효과적으로 해결할 수 있음을 시사한다. 본 연구는 수위 데이터의 품질을 향상시켜 수자원 관리 결정의 신뢰성에 기여하는 강력한 도구로서 Wavelet Transform의 잠재력을 확인한 의의가 있다.