• Korean Journal of Environmental Biology
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• v.39 no.4
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• pp.471-478
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• 2021

Effective microorganisms (EM), which are sources of fermentation byproducts in herbal medicine, were compared with oyster mushrooms(OM) to identify alternative larval feeds for white-spotted flower chafer (Protaetia brevitarsis seulensis). The nutritional composition of each fermented feed was analyzed. The content of crude protein, crude fat, and fiber was higher in the OM fermented feed, except for crude ash. No difference in nutritional content of larvae based on fermented feed was observed. A comparative weekly analysis of the live weights of larvae was based on the fermented feed used. The average weight was significantly higher in the feeds using EM and OM from third week of observation. The larval survival rate in the presence of fermented feed was 96.7% compared with 9.8% with non-fermented feed. The results suggest that fermented food source is essential for the growth of white-spotted flower chafer, and OM was a stable alternative to EM as a fermentation source for the survival of white-spotted flower chafer.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2004.05a
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• pp.135-137
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• 2004

유산균을 첨가한 발효육의 지방산패도를 측정하여 EM의 항산화 효과와의 관련성을 살펴보기 위해 실험을 실시하였다. 발효육의 생산에 사용된 Lactobacillus casei KCTC 3109 공시균주는 한국생명공학연구원 유전자지원센터 한국유전자은행에서 주문 구입하였다. 실험에 사용된 돈육은 도살 후, $4^{\circ}C$ 냉장고에 저장하였던 랜드레이스의 대퇴부 육을 사용하였다. 발효육의 항산화 정도를 알아보기 위해 TBA 방법으로 실험하였다. 대조구는 저장 0일부터 저장 3일까지 TBARS 치가 지속적으로 상승하는 것을 보였다. 그러나 저장 4일째부터는 TBARS 치가 감소하는 것을 볼 수 있다. 반면, 유산균을 첨가한 육에서는 저장 0일째부터 2일째까지는 약간 TBARS 값이 증가하였으나 저장 3일째는 가장 낮은 수치를 보였다. 그러나 저장 4일째부터는 TBARS 치가 점차적으로 증가하였다. 대조구의 저장 3일째의 TBARS치가 0.19 mgMDA/Kg을 나타낸 반면, 유산균을 첨가하여 $37^{\circ}C$에서 3일간 정치 배양한 발효육의 TBARS치는 0.02 mgMDA/Kg을 나타내 8배 정도의 항산화 효과를 나타냈다. 결론적으로 유산균 자체가 항산화 효과가 있음을 알게 되었고 EM의 항산화 효과에도 영향을 주는 것으로 사료된다.

• Korean Journal of Organic Agriculture
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• v.15 no.2
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• pp.207-218
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• 2007

Coming with the well-being era, consumer's demand for safe agricultural products is increasing. So, it is urgent to develop an environment-friendly rice production system. Accordingly, this study was conducted to develop an environment-friendly rice production system by using Amo known as EM(effective microorganisms) and also known as being effective in environment-friendly rice production with several other environment-friendly agricultural materials. The highest number of tillers per hill was obtained from level 2 of EM treatment (48.8 tillers per hill), while the lowest was obtained from the control plot (41.0 tillers per hill). Leaf area per hill at heading stage was the highest in level 3 of EM treatment $(3228.5cm^2)$, while control was the lowest leaf area $(2264.7cm^2)$, which is 70.2% compared to the level 3 of EM treatment. The highest effective tillers was obtained from the control (63.7%), while the lowest effective tillers was obtained from the level 3 of EM treatment (55.4%), which were treated with higher amounts of environment-friendly agricultural materials. Level 3 of EM treatment showed the highest number of panicles per hill (20.9), while the control showed the lowest number of panicles per hill (19.3). In the spikelets per panicle, level 2 of EM treatment showed the highest number of spikelets (85.2), while the control showed the lowest number of spikelets (81.9) and there was a statistically significant difference among the three levels and control. The highest grain filling ratio was obtained from the control (85.0%), while level 3 of EM treatment was the lowest grain filling ratio and there were no great difference between treatment levels. Regarding the 1000 grain weight, the control showed the highest 1000 grain weight (21.7g), which is heavier by about 1g compared to treatment levels. Level 2 of EM treatment showed the highest rough rice yield per 10a, while level 3 of EM treatment was the lowest and they also showed a statistically significant difference among treatment levels.

• Korean Journal of Soil Science and Fertilizer
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• v.40 no.4
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• pp.234-241
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• 2007

Biological amendments consisting of suspensions of selected microorganisms are often used in conjunction with various organic materials for amending soils to improve soil quality and plant growth. The effects of the biological amendment on chemical and biological properties of soil were investigated for a biological amendmentalone and when combined with different organic materials includingmunicipal compost (MC), poultry litter (PL), and cover crops (red clover (RC) and spring oats). A liquid preparation of a biological amendment called Effective Microorganisms was sprayed on the tested plots three times over a two-year period. Effective Microorganisms alone did not influence pH, K, or organic matter content in soil. However, increases in P in PL-treated soils in fall of both years andCa in MC-treated soil in fall 2001, and decreases in Ca, Mg, and cation exchange capacity (CEC) in RC-planted soil were associated with EM. Increased dehydrogenase(DH) activitiesassociated with Effective Microorganismswere only detected in July (P=0.0222) and October (P=0.0834) for RC-planted soils in the first year. Fluorescein diacetate (FDA) hydrolysisappeared to be enhanced by Effective Microorganisms in soils untreated or treated with MC and oatsbut only sporadically during the sampling period. FDA hydrolysis in both PL- and RC-treated soils as well as DH activity in PL-treated soils decreased with Effective Microorganisms treatment. Effective Microorganisms did not influence substrate utilization patterns expressed by the BIOLOG assay. We conclude that Effective Microorganisms effects on soil chemical and biological properties varied depending on the added organic materials. Effective Microorganisms periodically increased soil DH activity and FDA hydrolysis with RC and with MC plus oats, respectively.

• Journal of Environmental Science International
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• v.24 no.1
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• pp.55-64
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• 2015

The dry weight of mother plants' leaves had the highest increase rate in both NS (single-use) and NS+EM (mixed-use) mixed with NS 0.8 (customary use). In seafood amino acid fertilizer (SAF) application, the increase rate was highest in SAF solution at a 300-fold dilution. Mother plants' crown diameter, plant height, leaf length, leaf width, petiole length and leaf number showed the greatest growth amount when NS 0.8 (customary use) was mixed to NS (single-use) or NS+EM (mixed-use) solution. The growth was highest in SAF solution diluted 300 folds, but lowest in SAF solution diluted 100 folds. Of all inorganic nutrients, excluding sulfur, total amount of nitrogen, available phosphorus, potassium, calcium and magnesium had the highest increase rate in both NS (single-use) and NS+EM (mixed-use) with the treatment of NS 0.8 (customary use). Total nitrogen, in particular, was increased by 3.1% in NS 0.4, 6.0% in NS 0.8, and 4.5% in NS 0.8 with the application of NS+EM at a 500-fold dilution compared to NS alone. Total nitrogen amount showed the highest increase rate in SAF solution diluted 300 folds. Total nitrogen, available phosphorus, calcium, magnesium and EC in soils applied with culture solutions (NS, NS+EM) had increasing tendencies after fertilizer application. The results were comparable to those of SAF treatment. The increase rate of each inorganic nutrient composition declined in soils applied with NS+EM solution diluted 500 folds compared to NS alone.

• Korean Journal of Organic Agriculture
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• v.20 no.3
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• pp.313-326
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• 2012

The aim of this study was to determine the effect of effective microorganisms (EM) on growth, yield and fruit nutrient contents of two cultivars ('Muhanjilju' and 'Daetong') of hot pepper. The number of injection of EM cultivated are 6 times from the pepper plant seedlings until harvested in both cultivars. Stem girth was highest in the treatment of Lactobacillus plantarum whereas lowest in no EM control. In 'Muhanjilju', the number of branches was highest (79.3) in the treatment of Bacillus subtilis whereas in 'Daetong' was highest (119.0) as treated with Lactobacillus plantarum. Yield of the red hot pepper fruits that sum of two varieties was highest in the treatment of Rhodopseudomonas capsulatas (53.1kg FW per 10a). Regardless of EM treatments, average yield was 8% higher in 'Daetong' than is 'Muhanjilju' (33.8kg vs 31.2kg per 10a). The incidence of antracnose was lowest by the Rhodopseudomonas capsulatas treatment, which led to the highest yield. As for the effect on fruit mineral nutrients, total N and phosphorus concentrations were highest in the treatment of Lactobacillus plantarum and Bacillus subtilis in both varieties, respectively. The highest content of total carotenoids was obtained from the treatment of Lactobacillus plantarum in both varieties.

• Journal of the Korean Society of Industry Convergence
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• v.17 no.3
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• pp.170-177
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• 2014

This study was conducted to know the behavior of pH behavior in the tomato juices to find out an effective medium for microbial cultivation. Bacterial culture media is a material consist a mixture of nutrients used to grow microorganisms on or in it. In addition, microbial culture media can also be used for isolation, propagation, testing the nature physiological, and calculation of the number of microorganisms. Fresh tomato juice is used for basic ingredient, therein added salt, sugar and EM (Effective Microbial). The fermented solution placed in a room with a temperature of 40oC. Data retrieval before the pH value reached a constant value is done every 12 hours, after constant rate data collection was done every 24 hours. The pH value has been steady after 372 hours of fermentation process (15.5 days). From the results obtained that the amount of additional ingredient which added into tomato juice does not affect final pH value of solution. Thereby the most effective treatment for microbial cultivation media is treatment number four.

• Journal of Life Science
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• v.21 no.4
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• pp.584-588
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• 2011

This study was conducted to investigate the effects of organic materials, chitosan, wood vinegar, and EM active solution, on soil microbial and growth in Chinese cabbage. The organic materials were treated with chitosan, wood vinegar, and EM (effective microoganism) active solution, and the treatment concentration was 100 times solution and 1,000 times solution level with foliar application. The results are summarized as follows: Among foliar application of organic materials treatment plot[?], with 1,000 times solution level of chitosan was effective in inhibiting microbial growth in Chinese cabbage compared to other plots and control. The microorganism number in the soil for cultivation of Chinese cabbage increased with organic materials treatment plot as compared with control. Especially, 1,000 times solution level of chitosan showed the most significant effect.

• Journal of the Korea Organic Resources Recycling Association
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• v.27 no.4
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• pp.15-24
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• 2019

The study was compared for soil chemical and microbial properties as well as growth of the cherry tomato (Lycopersicon esculentum var. cerasiforme) plants environmentally friendly gown for 3 years and 5 years, which had been fertigated with homemade liquid fertilizer (LF) with indigenous microorganism as an additional fertilizer. Treatment included LF with indigenous microorganism for 3 years (3-year IM-LF) and for 5 years (5-year IM-LF), with an effective microorganism for 10 years (EM-LF), which had been applied with 1,000 times of dilution in the farmhouse. IM-LF and EM-LF materials had increased pH pattern for 16 weeks, in particular for increase of 1.2 for EM-LF. IM-LF material contained slightly higher EC but similar level of 0.2 dS/m to EM-LF. For a pot experiment in the greenhouse, IM-LF treatment increased root dry weight of the cherry tomato plants. In the farmhouse experiment, IM-LF treatment increased to 7.5 of soil pH and 8.4 dS/m of EC, indicating high salt accumulation. EM-LF treatment increased to 62 g/kg of soil OM, which would have affected concentrations of macro essential nutrients, including T-N in the soil. However, the optimum soil chemical levels for growth of cherry tomato plants were observed on the IM-LF plots. EM-LF treatment increased number of bacteria and actinobacteria in the soil. EM-LF treatment increased concentrations of macro essential nutrients in the plants, except for P, with similar nutrient concentrations observed between 3-year IM-LF and 5-year IM-LF-treated plants. Leaf SPAD and PS II levels decreased in the plants treated with 3-year IM-LF. EM-LF treatment increased leaf width and length, number of leaves, canopy area, plant height, and stem diameter in the mid-term stage of growth, which were not significantly different between the treatments. EM-LF treated-plants had two times higher leaf dry weight than those of values observed on the IM-LF plants, which was the opposite result observed on the number of fruit.

• Journal of Korean Society on Water Environment
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• v.33 no.4
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• pp.409-415
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• 2017

This study aims to evaluate the effect of capping materials on blocking pollutant elution from contaminated sediment to water body. Experiments were carried out under conditions in which the elution rate was intensified artificially using compost with high concentration of organic compound and nutrient salts instead of sediments. Activated carbon (AC), modified activated carbon (MAC), P. putida immobilized activated carbon (PBAC) and effective microorganisms immobilized activated carbon (EBAC) were used as capping materials. Zeolite (ZT) and two kinds of commercially available microorganisms immobilized zeolite products (ZC, ZN) were used for comparison experiment. The elution rate of organic compound, nitrogen and phosphorus were compared with that of control experiment. The experiments were conducted for 56 days. Concentrations of chemical oxygen demand, total nitrogen, and total phosphorus were measured to use the comparison of release rate of organic compound, nitrogen and phosphorus. From the experimental results, AC based materials showed better performance to block the elution of organic compound and nitrogen than ZT based materials. Although ZT based materials were more effective than AC and PBAC to block phosphorus, MAC and EBAC showed the best performance of phosphorus elution blocking among the all candidate materials. In conclusion, EBAC is considered as the most effective capping materials, because organic compound, nitrogen and phosphorus will be degraded continuously by EM in the long term.