• 대한의생명과학회지
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• 제9권3호
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• pp.145-150
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• 2003

In the intestinal mucosa, mast cells are thought to be responsible for the expulsion of parasites. We investigated the relationship of worm expulsion and mast cells in C3H/HeN and BALB/c mice infected with Echinostoma hortense. In addition, we examined whether the worm recovery rate was associated with the strain of mice, and whether a toluidine stain and immunohistochemistry using the c-kit antibody was effective in the detection of mast cells. In order to investigate the mucosal immune response of C3H/HeN and BALB/c mice, each mouse was infected orally with 30 E. hortense metacercariae. Then, the number of mucosal mast cells and worm recovery rates was observed in experimentally infected mouse strains between 1 week and 8 weeks post infection (PI). Mucosal mast cells were increased in 3 weeks P.I. in C3H/HeN and BALB/c mice. On the other hand, only mucosal goblet cells and worm recovery rates correlated in C3H/HeN mice (P=0.0482). Worm recoveries in C3H/HeN mice were 65.7$\pm$5.6, 53.3$\pm$5.4 and 6.7$\pm$0.6 in week 1, 2, and 3 P.I. and strongly decreased in week 3 P.I. Worm recoveries in BALB/c mice were 23.0$\pm$2.5, 10.0$\pm$1.0, and 6.7$\pm$0.6% in week 1, 2, and 3 P.I. and gradually decreased from week 1 P.I. to week 3 P.I. Worm recoveries in C3H/HeN mice were significantly higher than in BALB/c mice (P<0.00l). The number of mast cells in C3H/HeN and BALB/c mice using the anti-c-kit antibody reached to a peak in week 3 P.I. and recovered as normal level in week 5 P.I. and 6 P.I. The number in E. hortense-infected C3H/HeN mice (P=0.0015) was higher than in E. hortense-infected BALB/c mice (P=0.01) compared with the control group. There were significant differences in the number of mast cells among regions of the intestine in in C3H/HeN mice (P<0.05) but not in BALB/c mice (P>0.05). Immunohistochemistry using the anti-c-kit antibody was significant method as an examination of the number of mast cells (P=0.0002). In conclusion, the present study demonstrated that mast cells play an important role in worm recovery, and immunohistochemistry using the anti-c-kit antibody was superior to toluidine stain as an examination of mast cells.

• Journal of Animal Science and Technology
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• 제47권6호
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• pp.985-1000
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• 2005

리놀렌산(C18:3)과 리놀산(C18:2)의 biohydrogenation 과 반추위 내에서의 이들 지방산의 bypass를 검토하기 위하여 3 구로 나누어 티모시 조사료를 반추위액과 함께 in vitro상에서 39℃, 0, 3, 6, 9, 24, 36 시간 동안 혐기 배양 시험을 하였다. 첫번째 시험의 목적은 C18:2과 C18:3의 in vitro biohydrogenation에 대한 조사료의 성장 단계(stem elongation, early heading, late heading, early flowering)와 질소 시비(0 and 120kg N ha－1)의 영향을 시험하는 것이었다. 수소첨가가능분획(hydrogenable fraction), C18:2과 C18:3의 효과적인 소실과 bypass는 stem elongation 시 수확된 티모시에서 높았고, 성숙함에 따라 일정하게 감소하였다. 질소시비구는 C18:3의 수소첨가가능분획, C18:2과 C18:3의 효과적인 소실과 bypass가 증가하였다. 그러나 C18:2과 C18:3의 소실율은 성숙과 질소시비(P≻0.1)에 의해 영향을 받지 않았다. 2번째 시험에서는 in vitro C18:2과 C18:3 biohydrogenation에 대한 티모시 보존 방법의 영향을 보았다. 사일리지는 C18:2과 C18:3를 가장 효과적으로 소실시켰으며, 건초는 가장 효과가 낮았다. C18:2과 C18:3의 biohydrogenation 된 양은 건초에서 보다 헤일리지와 사일리지에서 더 높았다. 티모시 헤일리지와 비교하였을 때 C18:3의 bypass는 신선 목초, 시든풀, 건초에서 더 높았다. C18:2의 bypass는 건초와 헤일리지에 비해 신선목초와 사일리지가 더 높았다. 3번째 시험에서는 C18:2과 C18:3의 소거와 bypass에 대한 티모시 헤일리지와 사일리지에 대한 개미산 첨가와 Lactobacillus plantarum 접종의 효과를 검토하였다. 개미산의 첨가는 헤일리지와 사일리지에 있어서 C18:3의 biohydrogenation 비율을 증가시켰으나 사일리지에 있어서 C18:2의 수소첨가 가능분획을 감소시켰다. 이러한 3가지 배양구의 결과는 티모시에 있어 C18:2과 C18:3의 수소첨가 가능분획과 bypass가 성숙도에 따라 감소하였고 질소시비에 따라 증가하였음을 보여 준다. 헤일리지와 사일리지에서 건초에서 보다 C18:2과 C18:3의 더 많은 양이 biohydrogenation 되었으며 C18:3의 반추위 소실은 헤일리지에서 보다 신선목초, 시든풀, 건초에서 더 높았다.

• 한국미생물·생명공학회지
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• 제31권2호
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• pp.171-176
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• 2003

생물계면활성제 생성균주, Pseudomonas aeruginosa F722를 이용하여 다양한 배양조건과 배지조성에서 생물계면활성제 생산성을 검토하였다. 질소원과 탄소원을 검토하기 전에는 P. aeruginosa F722의 생물계면활성제 생산량은 0.78 g/l이었다. 하지만, 질소원과 탄소원을 검토한 후에는 생물계면활성제 생산량이 2배 증가한 1.66g/l이었다. 무기질소원으로 $_NH4$Cl 또는 $NaNO_2$를 첨가하였을 때 생물계면활성제 활성에 효과적이었으며 유기질소원으로는 yeast extract 또는 tryptone을 첨가하였을 때 생물계면활성제 활성이 높았다. 이중 무기 질소원으로 0.05% $NH_4$Cl , 유기 질소원으로 0.1% yeast extract를 질소원으로 첨가하였을 때 가장 최적이었다. 탄소원으로 소수성 기질(n-alkane) 또는 친수성 기질(glucose, glycol)을 첨가하여 생물계면활성제 생산량을 조사하였는데 소수성 기질보다는 친수성 기질인 3.0% glucose를 첨가하였을 때 생물계면활성제 생산량이 높았다. 이때의 탄소원/질소원 비율은 17~20이었다. P. aeruginosa F722는 배양조건 3.0% glucose, 0.05% $NH_4$Cl, 0.1% yeast extract, $35^{\circ}C$, pH 7.0, C/N ratio 20, 5 days에서 생물계면활성제 생산량은 1.66g/l이였다. 질소원이 결핍 후 탄소원을 첨가하여 배양하였을 때가 질소원과 탄소원을 함께 첨가하여 배양했을 때보다 생물계면활성제 생산속도 및 균체 생장속도가 높았다. 최적 배양조건하에서 얻어진 배양액의 표면장력은 30mN/m이었다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권2호
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• pp.176-182
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• 2019

Objective: This study was conducted to investigate basic information on genetic structure and characteristics of Limousin population in Hungary. Obtained results will be taken into consideration when adopting the new breeding strategy by the Association of Hungarian Limousin and Blonde d'Aquitaine Breeders (AHLBB). Methods: Genetic diversity and phylogenetic relationship of 3,443 Limousin cattle from 16 different herds were investigated by performing genotyping using 18 microsatellite markers. Amplified DNA was genotyped using an automated genetic analyzer. Results: Mean of effective alleles ($n_e$) of the populations was 3.77. Population C had the lowest number of effective alleles (3.01) and the lowest inbreeding coefficient ($F_{IS}$) value (-0.15). Principal component analysis of estimated genetic distance ($F_{ST}$) values (p<0.000) revealed two herds (C and E) distinct from the majority of other Limousin herds. The pairwise $F_{ST}$ values of population C compared to the others (0.066 to 0.120) fell into the range of moderate genetic distance: 0.050 to 0.150, while population E displayed also moderate genetic distance ($F_{ST}$ values in range 0.052 to 0.064) but only to six populations (G, H, J, L, N, and P). $F_{ST(C-E)}$ was 0.148, all other pairs -excluding C and E herds- displayed low genetic distance ($F_{ST}$<0.049). Population D, F, I, J, K, L, N, O, and P carried private alleles, which alleles belonged to 1.1% of the individuals. Most probable number of clusters (K) were 2 and 7 determined by Structure and BAPS software. Conclusion: This study showed useful genetic diversity and phylogenetic relationship data that can be utilized for the development of a new breeding strategy by AHLBB. The results presented could also contribute to the proper selection of animals for further whole genome scan studies of Limousins.

• 한국자기학회지
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• 제2권3호
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• pp.239-243
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• 1992

$ThMN_{12}$ 구조를 갖는 $NdFe_{11}TiN_x$ 형 화합물에서 B 이 자화 및 큐리온도의 상승에 매우 효과적임이 밝혀졌다. $NdFe_{10.7}TiB_{0.3}N_x$ 의 자화 및 큐리온도는 $NdFe_{10.7}Ti_{1.3}N_x$ 의 경우에 비하여 각각 20 $Am^2$/kg (20 emu/g) 및 $90^{\circ}C$가 높은 148 $Am^2$/kg (148 emu/g) 및 $560^{\circ}C$를 나타내었다. 한편, Mo은 이방성자장의 증가에 효과적이며, 또한, 질화처리시 ${\alpha}-Fe$의 생성 억제에 효과적인 것으로 추측된다. $NdFe_{10.7}TiMo_{0.3}N_x$ 의 이방성자장은 약 7960 kA/m (100 kOe) 로 $NdFe_{10.7}Ti_{1.3}N_x$ 의 경우에 비하여 약 1590 kA/m (20 kOe) 가 증가한 결과를 보였다.

• 터널과지하공간
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• 제7권3호
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• pp.238-245
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• 1997

A transient pulse methos has been used to measure the permeability of Pocheon granite pre-heated from $25^{\circ}C$ to $600^{\circ}C$ at effective pressure up to 32MPa. The permeability of whole rock ranged from 0.72 $\mu$d at 10MPa to 0.20 $\mu$d at 32MPa. The permeability of rock heated to $600^{\circ}C$ ranged from 18.07$\mu$d at 10MPa to 6.39$\mu$d at 32MPa. Confining pressure has greater effects on the rocks thermally treated to lower thermal-cycle temperatures than on the higher thermally treated rocks. The increase of permeability is most pronounced between 40$0^{\circ}C$ and $600^{\circ}C$. Below 40$0^{\circ}C$, permeability increase is expected to be associated with the formation of new cracks and widening of preexisting cracks, whereas above 40$0^{\circ}C$, permeability increase is expected to reflect widening of cracks. Using the equivalent channel model, author shows that the exponent n in the relationship relating the permebility(k) to porosity($\phi$) by k∝$$\phi$^n$ falls in the range 2.7$\leq$n$\leq$3.0.

• Journal of Positioning, Navigation, and Timing
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• 제10권1호
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• pp.21-28
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• 2021

In order to develop a Korea Positioning System (KPS) as part of key national infrastructure, independent navigation signal design is essential. The designed signal candidates must coexist with existing or planned GNSS signals within the limited frequency band. This requires a RF compatibility assessment, which can be performed using the Spectral Separation Coefficient (SSC) and Effective Carrier to Noise Density Ratio (Effective C/N0), for navigation signals. Thus, in this paper, the analysis of RF compatibility between the designed signal candidates and the existing GNSS signals is carried out based on analytical and numerical techniques.

• Applied Biological Chemistry
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• 제40권4호
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• pp.294-300
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• 1997

수산가공 부산물을 식용자원으로 이용하기 위한 일련의 연구로서 오징어 내장유의 정제조건에 대하여 검토하였다. 오징어 내장유 100ml에 대하여 인산 3ml를 첨가하고 $60^{\circ}C$에서 30분간 교반하여 탈검한 다음 이것에 20% 수산화나트륨을 0.6% 과잉첨가한 후 탈산처리 하는 것이 효과적이었다. 탈산유에 대하여 10%에 해당하는 활성백토를 가한 후 감압하에 $100^{\circ}C$에서 20분간 탈색처리한 후 압력을 4torr 이하의 감압하에서 수증기증류법으로 $180^{\circ}C$에서 60분간 처리한 오징어 내장유가 물리, 화학적 성상이 가장 우수하였다. 정제유의 산값, 과산화물값 및 색도는 각각 0.20, 0.8 meq/kg 및 0.019이었다. 정제유의 포화지방산에 대한 고도불포화지방산의 비율은 1.28이었고, 주요 구성 지방산은 16 : 0, 18 : 1n-9, 20 : 5n-3 및 22 : 6n-3 등으로 기능성이 우수하였다.

• Biotechnology and Bioprocess Engineering:BBE
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• 제11권6호
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• pp.471-476
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• 2006

Pseudomonas aeruginosa F722 produces a biosurfactant (BS) during its degradation of carbon and hydrocarbon compounds. The culture conditions for upgrading the biosurfactant productivity were investigated. The concentration of the biosurfactant produced by P. aeruginosa F722 was 0.78 g/L in C-medium; however, this increased to 1.66 g/L in BS medium, which was experimentally adjusted to optimal conditions. $NaNO_{2}$ was found to be most effective for microbial growth, with an $O.D_{600nm}$ of 1.18 for 0.1 % $NaNO_{2}$. Microbial growths, according to the $O.D_{600nm}$ were 2.53, 2.68, 2.89, and 2.87 for glucose, glycerol, $n-C_{10},\;and\;n-C_{22}$, respectively. Clear zone diameters (cm), indicating biosurfactant activity, were 9.0, 8.8, 5.7, and 8.5 for glucose, glycerol, $n-C_{10},\;and\;n-C_{22}$, respectively. Microbial growth was not consistent with the biosurfactant activity. The best biosurfactant activity was found with a C/N ratio of 20. Under optimal culture condition, the average surface tension decreased from 70 to 30 mN/m after 5 days. With aeration of 1.0 vvm, the biosurfactant produced increased to 1.94 g/L (up to 20%) compared to that of 1.66 g/L with no aeration. With aeration, the velocities of glucose degradation during both the log and stationary growth phases increased from 0.25 and $0.18\;h^{-1}$ to 0.33 and $0.29\;h^{-1}$, respectively, and the time for the culture to arrive at the maximum clear zone diameter became shorter, from 80 down to 60 h with no aeration.

• 한국자기학회지
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• 제5권5호
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• pp.519-523
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• 1995

The Fe-TM-C-N nanocrystalline films (TM : Hf, Zr and Nb) are investigated to examine the relation between microstructure and soft magnetic properties. In these films, as the atomic radius of TM element increases, $P_{N2}$ which was added to get good soft magnetic properties was decreased and the maximum value of the permeability shifted to the high Fe range in the composition diagram. The best soft magnetic properties achieved in these films are : Hc of 0.15 Oe, $\mu_{eff}$ of 7800 (1MHz) and $4{\pi}M_{s}$ of 17.5 kG in Fe-Hf-C-N film ; Hc of 0.06 Oe, $\mu_{eff}$ of 2750 (1MHz) and $4{\pi}M_{s}$ of 16.8 kG in Fe-Zr-C-N film and Hc of 0.31 Oe; $\mu_{eff}$ of 2100 (1MHz) and $4{\pi}M_{s}$ of 15.5 kG in Fe-Nb-C-N film. It was considered that the stronger the bonding force between TM and C(N), the finer TM(C,N) phase is precipitated and therefore, the finer $\alpha$-Fe grains are formed. The effective permeability of the Fe-Zr-C-N films and Fe-Nb-C-N films remains nearly constant up to 10 MHz.