• BMB Reports
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• 제54권2호
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• pp.98-105
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• 2021

The clustered regularly interspaced short palindromic repeats (CRISPR) system is a family of DNA sequences originally discovered as a type of acquired immunity in prokaryotes such as bacteria and archaea. In many CRISPR systems, the functional ribonucleoproteins (RNPs) are composed of CRISPR protein and guide RNAs. They selectively bind and cleave specific target DNAs or RNAs, based on sequences complementary to the guide RNA. The specific targeted cleavage of the nucleic acids by CRISPR has been broadly utilized in genome editing methods. In the process of genome editing of eukaryotic cells, CRISPR-mediated DNA double-strand breaks (DSB) at specific genomic loci activate the endogenous DNA repair systems and induce mutations at the target sites with high efficiencies. Two of the major endogenous DNA repair machineries are non-homologous end joining (NHEJ) and homology-directed repair (HDR). In case of DSB, the two repair pathways operate in competition, resulting in several possible outcomes including deletions, insertions, and substitutions. Due to the inherent stochasticity of DSB-based genome editing methods, it was difficult to achieve defined single-base changes without unanticipated random mutation patterns. In order to overcome the heterogeneity in DSB-mediated genome editing, novel methods have been developed to incorporate precise single-base level changes without inducing DSB. The approaches utilized catalytically compromised CRISPR in conjunction with base-modifying enzymes and DNA polymerases, to accomplish highly efficient and precise genome editing of single and multiple bases. In this review, we introduce some of the advances in single-base level CRISPR genome editing methods and their applications.

• Molecules and Cells
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• 제41권8호
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• pp.717-723
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• 2018

Chimeric antigen receptor (CAR) T-cell therapy, an emerging immunotherapy, has demonstrated promising clinical results in hematological malignancies including B-cell malignancies. However, accessibility to this transformative medicine is highly limited due to the complex process of manufacturing, limited options for target antigens, and insufficient anti-tumor responses against solid tumors. Advances in gene-editing technologies, such as the development of Zinc Finger Nucleases (ZFNs), Transcription Activator-Like Effector Nucleases (TALENs), and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR/Cas9), have provided novel engineering strategies to address these limitations. Development of next-generation CAR-T cells using gene-editing technologies would enhance the therapeutic potential of CAR-T cell treatment for both hematologic and solid tumors. Here we summarize the unmet medical needs of current CAR-T cell therapies and gene-editing strategies to resolve these challenges as well as safety concerns of gene-edited CAR-T therapies.

• Clinical and Experimental Pediatrics
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• 제64권6호
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• pp.269-279
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• 2021

Clustered regularly interspaced short palindromic repeats and CRISPR-associated protein 9 (CRISPR-Cas9) is an ancient prokaryotic defense system that precisely cuts foreign genomic DNA under the control of a small number of guide RNAs. The CRISPR-Cas9 system facilitates efficient double-stranded DNA cleavage that has been recently adopted for genome editing to create or correct inherited genetic mutations causing disease. Congenital heart disease (CHD) is generally caused by genetic mutations such as base substitutions, deletions, and insertions, which result in diverse developmental defects and remains a leading cause of birth defects. Pediatric CHD patients exhibit a spectrum of cardiac abnormalities such as septal defects, valvular defects, and abnormal chamber development. CHD onset occurs during the prenatal period and often results in early lethality during childhood. Because CRISPR-Cas9-based genome editing technology has gained considerable attention for its potential to prevent and treat diseases, we will review the CRISPR-Cas9 system as a genome editing tool and focus on its therapeutic application for CHD.

• 정보교육학회논문지
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• 제11권1호
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• pp.107-116
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• 2007

전자펜을 이용한 문서교정 시스템에서 정확한 교정결과를 보장하기 위해서는 문서 교정자가 드로잉한 교정부호와 문서내용간의 영역 모호성(ambiguity)을 해결하여야 한다. 한편 교정의 대상이 되는 전자문서가 HTML/XML과 같은 경우 교정된 문서구조가 반드시 기 정의된 DTD를 위배하지 않아야 한다. 본 논문에서는 펜 기반의 교정시스템에서 교정부호(마킹)와 대상문서간의 모호성 문제를 최소화하기 위한 기법을 제안한다. 제안 인터페이스에서는 모호성 문제를 최소화하기 위하여 교정부호와 문서간의 컨텍스트(Context)를 반영하였으며 동시에 대상문서의 문서 구조를 유지하기 위한 방법을 제공한다. 그 결과 본 논문에서 제안한 교정 인터페이스는 기존 교정시스템에 비하여 보다 정확한 영역정보를 포함할 수 있으며, 교정부호 입력에 따른 구조문서 변경시에도 원본문서의 DTD에 따르는 문서구조를 유지할 수 있다.

• 한국산학기술학회논문지
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• 제6권3호
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• pp.241-248
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• 2005

MPEG-7은 디지털 영상과 오디오와 같은 멀티미디어 데이터에 대한 구조와 의미 정보를 제공해 줌으로써, 정보를 효율적으로 검색하고 탐색하는데 도움을 주고 있다. 본 논문에서는 MPEG-7 기반의 메타데이터 편집할 수 있는 소프트웨어 시스템을 소개하고자 하며 이는 대화형 컨텐츠제작시에 필요한 메타데이터의 편집에 필요한 기술 및 전체 프레임웍을 제공한다. 본 연구에서 개발한 시스템은 MPEG-7 DDL을 기반으로 하는 메타데이터 생성, 편집, 저장 기능, MPEG1, 2 동영상을 기반으로 하는 동영상의 메타데이터 편집 기능, 그리고 편집과정 전체를 브라우징 할 수 있는 기능도 제공한다.

• International Journal of Computer Science & Network Security
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• 제22권3호
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• pp.312-318
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• 2022

From the past two eras, artificial intelligence has gained the attention of researchers of all research areas. Video editing is a task in the list that starts leveraging the blessing of Artificial Intelligence (AI). Since AI promises to make technology better use of human life although video editing technology is not new yet it is adopting new technologies like AI to become more powerful and sophisticated for video editors as well as users. Like other technologies, video editing will also be facilitated by the majestic power of AI in near future. There has been a lot of research that uses AI in video editing, yet there is no comprehensive literature review that systematically finds all of this work on one page so that new researchers can find research gaps in that area. In this research we conducted a statically approach called, systematic mapping study, to find answers to pre-proposed research questions. The aim and objective of this research are to find research gaps in our topic under discussion.

• BMB Reports
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• 제56권2호
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• pp.102-107
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• 2023

Genome editing using CRISPR-associated technology is widely used to modify the genomes rapidly and efficiently on specific DNA double-strand breaks (DSBs) induced by Cas9 endonuclease. However, despite swift advance in Cas9 engineering, structural basis of Cas9-recognition and cleavage complex remains unclear. Proper assembly of this complex correlates to effective Cas9 activity, leading to high efficacy of genome editing events. Here, we develop a CRISPR/Cas9-RAD51 plasmid constitutively expressing RAD51, which can bind to single-stranded DNA for DSB repair. We show that the efficiency of CRISPR-mediated genome editing can be significantly improved by expressing RAD51, responsible for DSB repair via homologous recombination (HR), in both gene knock-out and knock-in processes. In cells with CRISPR/Cas9-RAD51 plasmid, expression of the target genes (cohesin SMC3 and GAPDH) was reduced by more than 1.9-fold compared to the CRISPR/Cas9 plasmid for knock-out of genes. Furthermore, CRISPR/Cas9-RAD51 enhanced the knock-in efficiency of DsRed donor DNA. Thus, the CRISPR/Cas9-RAD51 system is useful for applications requiring precise and efficient genome edits not accessible to HR-deficient cell genome editing and for developing CRISPR/Cas9-mediated knockout technology.

• Journal of Veterinary Science
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• 제25권1호
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• pp.10.1-10.11
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• 2024

In livestock industry, there is growing interest in methods to increase the production efficiency of livestock to address food shortages, given the increasing global population. With the advancements in gene engineering technology, it is a valuable tool and has been intensively utilized in research specifically focused on human disease. In historically, this technology has been used with livestock to create human disease models or to produce recombinant proteins from their byproducts. However, in recent years, utilizing gene editing technology, cattle with identified genes related to productivity can be edited, thereby enhancing productivity in response to climate change or specific disease instead of producing recombinant proteins. Furthermore, with the advancement in the efficiency of gene editing, it has become possible to edit multiple genes simultaneously. This cattle breed improvement has been achieved by discovering the genes through the comprehensive analysis of the entire genome of cattle. The cattle industry has been able to address gene bottlenecks that were previously impossible through conventional breeding systems. This review concludes that gene editing is necessary to expand the cattle industry, improving productivity in the future. Additionally, the enhancement of cattle through gene editing is expected to contribute to addressing environmental challenges associated with the cattle industry. Further research and development in gene editing, coupled with genomic analysis technologies, will significantly contribute to solving issues that conventional breeding systems have not been able to address.

• 한국동물생명공학회지
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• 제38권4호
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• pp.247-253
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• 2023

Revolutionary advancements, such as the reduction in DNA sequencing costs and genome editing, have transformed biotechnology, fostering progress in manipulating biomolecules, engineering cells, and computational biology. Agriculture and food production have significantly benefited from tools like high-throughput microarrays, accelerating the selection of desired traits. Genetic engineering, especially utilizing genome editing, facilitates precise alterations in plants and animals, harnessing microbiomes and fostering lab-grown meat production to alleviate environmental pressures. The emergence of new biotechnologies, notably genome editing, underscores the necessity for regulatory frameworks governing LM (living modified) organisms. Global regulations overseeing genetically engineered or genome-edited (GE) organisms, encompassing animals, exhibit considerable diversity. Nonetheless, prevailing international regulatory trends typically exclude genomeedited plants and animals, employing novel biotechnological techniques, from GMO/ LMO classification if they lack foreign genes and originate through natural mutations or traditional breeding programs. This comprehensive review scrutinizes ongoing risk and safety assessment cases, such as genome-edited beef cattle and fish in the USA and Japan. Furthermore, it investigates the limitations of existing regulations related to genome editing in Korea and evaluates newly proposed legislation, offering insights into the future trajectory of regulatory frameworks.

• 한국컴퓨터정보학회논문지
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• 제25권5호
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• pp.1-9
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• 2020

본 논문에서는 머리카락과 모자 영역의 마스크 정보를 활용하여 더 자연스러운 얼굴 속성 편집(facial attribute editing)을 수행하는 모델을 제안한다. 최신 얼굴 속성 편집 연구인 STGAN은 다중 얼굴 속성을 자연스럽게 편집하는 성과를 보였다. 그러나 머리카락과 관련된 속성을 편집할 때 부자연스러운 결과를 생성할 수 있다. 제안하는 방법의 핵심 아이디어는 기존 모델에서 부족했던 얼굴 영역의 정보를 모델에 추가로 반영하는 것이다. 이를 위해 세 가지 아이디어를 적용한다. 첫째로 마스크를 통해 머리카락 면적 속성을 추가하여 머리카락 정보를 보완한다. 둘째로 순환 일관성 손실(cycle consistency loss)을 추가하여 영상의 불필요한 변화를 억제한다. 셋째로 모자 분할 신경망을 추가하여 모자 영역 왜곡을 방지한다. 정성적 평가를 통해 제안하는 방법 적용 여부에 따른 유효성을 평가 및 분석한다. 실험 결과에서 제안하는 방법이 머리카락 및 얼굴 영역을 더 자연스럽게 생성하고, 모자 영역의 왜곡을 성공적으로 방지했다.