• Title/Summary/Keyword: editing

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Determination of the Length of Target Recognition Sequence in sgRNA Required for CRISPR Interference (CRISPR 간섭에 필요한 sgRNA 표적 인식 서열 길이의 결정)

  • Kim, Bumjoon;Kim, Byeong Chan;Lee, Ho Joung;Lee, Sang Jun
    • Microbiology and Biotechnology Letters
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    • v.49 no.4
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    • pp.534-542
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    • 2021

  • Single-molecular guide RNA (sgRNA) plays a role in recognizing the DNA target sequence in CRISPR technology for genome editing and gene expression control. In this study, we systematically compared the length of the target recognition sequence in sgRNAs required for genome editing using Cas9-NG (an engineered Cas9 recognizing 5'-NG as PAM sequence) and gene expression control using deactivated Cas9-NG (dCas9-NG) by targeting the gal promoter in E. coli. In the case of genome editing, the truncation of three nucleotides in the target recognition sequence (TRS) of sgRNA was allowed. In gene expression regulation, we observed that target recognition and binding were possible even if eleven nucleotides were deleted from twenty nucleotides of the TRS. When 4 or more nucleotides are truncated in the TRS of the sgRNA, it is thought that the sgRNA/Cas9-NG complex can specifically bind to the target DNA sequence, but lacks endonuclease activity to perform genome editing. Our study will be helpful in the development of artificial transcription factors and various CRISPR technologies in the field of synthetic biology.

  • https://doi.org/10.48022/mbl.2111.11003 인용 PDF KSCI

A Study on How Governance of Genetic Scissors CRISPR-Cas9 for Research on Embryos Can Encourage a Researcher to Have a Sense of Responsibility - Focus on the Bioethics and Safety Act Article 47 - (유전자가위 CRISPR-Cas9을 이용한 인간 배아 연구에 있어서 연구자의 책임의식 고양을 위한 거버넌스 -개정 생명윤리 및 안전에 관한 법률 제47조를 중심으로-)

  • Kim, Minsung
    • The Korean Society of Law and Medicine
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    • v.23 no.1
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    • pp.121-148
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    • 2022

  • CRISPR-Cas9 is one of the gene-editing technologies that infinite potential. It may provide human beings with many benefits or cause unanticipated challenges. The governance as standards setting or regulation of gene-editing technologies can contribute to keeping a balance between scientific value and ethical commitments. Guaranteeing public participation provides an additional opportunity to think about ethical and moral considerations: For whose benefit the internationally discussed governance of gene-editing technologies is directed at? There is a doubt regarding whether the governance justifies scientific researchers' gene-editing research. Suppose that governance promotes the advancement of CRISPR-Cas9, it should also encourage greater research responsibility. If not, there may be tragedies brought about by the misconduct of researchers. Thus, the essential matter on the governance for the research of CRISPR-Cas9 is the researchers' responsibility.

  • https://doi.org/10.29291/kslm.2022.23.1.121 인용 PDF

Highly efficient genome editing via CRISPR-Cas9 ribonucleoprotein (RNP) delivery in mesenchymal stem cells

  • A Reum Han;Ha Rim Shin;Jiyeon Kweon;Soo Been Lee;Sang Eun Lee;Eun-Young Kim;Jiyeon Kweon;Eun-Ju Chang;Yongsub Kim;Seong Who Kim
    • BMB Reports
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    • v.57 no.1
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    • pp.60-65
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    • 2024

  • The CRISPR-Cas9 system has significantly advanced regenerative medicine research by enabling genome editing in stem cells. Due to their desirable properties, mesenchymal stem cells (MSCs) have recently emerged as highly promising therapeutic agents, which properties include differentiation ability and cytokine production. While CRISPR-Cas9 technology is applied to develop MSC-based therapeutics, MSCs exhibit inefficient genome editing, and susceptibility to plasmid DNA. In this study, we compared and optimized plasmid DNA and RNP approaches for efficient genome engineering in MSCs. The RNP-mediated approach enabled genome editing with high indel frequency and low cytotoxicity in MSCs. By utilizing Cas9 RNPs, we successfully generated B2M-knockout MSCs, which reduced T-cell differentiation, and improved MSC survival. Furthermore, this approach enhanced the immunomodulatory effect of IFN-r priming. These findings indicate that the RNP-mediated engineering of MSC genomes can achieve high efficiency, and engineered MSCs offer potential as a promising therapeutic strategy.

  • https://doi.org/10.5483/BMBRep.2023-0113 인용 PDF

회귀분석을 이용한 Data Editing

  • 허문열
    • Journal of the Korean Operations Research and Management Science Society
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    • v.5 no.1
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    • pp.89-90
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    • 1980

  • 원래 다량자료의 정리(Large Scale Data Screening)는 어떤 정립된 이론에 의해 수행되는 것보다는 그 자료자체가 가지는 성격과 자원의 Availability 등을 고려하여 수행되는 것이 상례이다. 여기서는 여러 방법 중 자료가 모두 수치로 나타나는 경우 자료정리의 한 유용한 방법으로 회귀분석을 사용하는 방법에 대해 설명코자 한다.

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