• Title/Summary/Keyword: editing

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A Study on GPS-Van Application for Editing and Updating Digital Map of Road System (도로기반 수치지도의 수정 및 갱신을 위한 GPS-Van 적용에 관한 연구)

  • Joo, Young-Eun;Lee, Hyung-Seok
    • Journal of the Korean Association of Geographic Information Studies
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    • v.8 no.3
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    • pp.129-141
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    • 2005

  • It is difficult to edit large-scale digital maps because of problems of cost and process and it is carried out by aerial photogrammetry in renewal periods. Five years of update period cannot provide exact data required in the fast-moving age. This study is to analyze applicability and impact for editing digital map of road system using the GPS-Van. Results are compared with accuracy of the data acquisition with GPS-Van positioning. INS data are affected by the barrier to receive GPS data. But high accuracy were achieved by thorough plans according to work order. By using GPS Van and fieldwork at the same time for editing and renewal of digital map, it is expected that this method can be used to reduce costs in the economic and temporal aspects, and provide rapid and accurate digital map of road system.

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Targeted Editing of Myostatin Gene in Sheep by Transcription Activator-like Effector Nucleases

  • Zhao, Xinxia;Ni, Wei;Chen, Chuangfu;Sai, Wujiafu;Qiao, Jun;Sheng, Jingliang;Zhang, Hui;Li, Guozhong;Wang, Dawei;Hu, Shengwei
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.3
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    • pp.413-418
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    • 2016

  • Myostatin (MSTN) is a secreted growth factor expressed in skeletal muscle and adipose tissue that negatively regulates skeletal muscle mass. Gene knockout of MSTN can result in increasing muscle mass in sheep. The objectives were to investigate whether myostatin gene can be edited in sheep by transcription activator-like effector nucleases (TALENs) in tandem with single-stranded DNA oligonucleotides (ssODNs). We designed a pair of TALENs to target a highly conserved sequence in the coding region of the sheep MSTN gene. The activity of the TALENs was verified by using luciferase single-strand annealing reporter assay in HEK 293T cell line. Co-transfection of TALENs and ssODNs oligonucleotides induced precise gene editing of myostatin gene in sheep primary fibroblasts. MSTN gene-edited cells were successfully used as nuclear donors for generating cloned embryos. TALENs combined with ssDNA oligonucleotides provide a useful approach for precise gene modification in livestock animals.

  • https://doi.org/10.5713/ajas.15.0041 인용 PDF KSCI