• Title/Summary/Keyword: eco-friendly agent

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Antifungal Activity of Green and Chemically Synthesized ZnO Nanoparticles against Alternaria citri, the Causal Agent Citrus Black Rot

  • Hazem S. Elshafie;Ali Osman;Mahmoud M El-Saber;Ippolito Camele ;Entsar Abbas
    • The Plant Pathology Journal
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    • v.39 no.3
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    • pp.265-274
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    • 2023
  • Citrus black rot is a serious disease of citrus plants caused by Alternaria citri. The current study aimed to synthesize zinc oxide nanoparticles (ZnO-NPs) by chemically or green method and investigate their antifungal activity against A. citri. The sizes of synthesized as measured by transmission electron microscope of ZnO-NPs were 88 and 65 nm for chemical and green methods, respectively. The studied prepared ZnO-NPs were applied, in vitro and in situ, at different concentrations (500, 1,000, and 2,000 ㎍/ml) in post-harvest treatment on navel orange fruits to verify the possible control effect against A. citri. Results of in vitro assay demonstrated that, at concentration 2,000 ㎍/ml, the green ZnO-NPs was able to inhibit about 61% of the fungal growth followed by 52% of chemical ZnO-NPs. In addition, scanning electron microscopy of A. citri treated in vitro with green ZnO-NPs showed swelling and deformation of conidia. Results showed also that, using a chemically and green ZnO-NPs at 2,000 ㎍/ml in situ in post-harvest treatment of orange, artificially-infected with A. citri, has reduced the disease severity to 6.92% and 9.23%, respectively, compared to 23.84% of positive control (non-treated fruits) after 20 days of storage. The out findings of this study may contribute to the development of a natural, effective, and eco-friendly strategy for eradicating harmful phytopathogenic fungi.

Field Applicability and Manufacturing of Foam Concrete as Filler with the Low-strength and High-flow for Repair System of Ground Subsidence (지반 함몰 복구용 저강도·고유동 충전재로서 기포콘크리트 연구 및 현장적용)

  • Ma, Young;Kim, Beom-Seok;Woo, Yang-Yi;Jung, Kyung-Hun;Song, Hun-Young
    • Resources Recycling
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    • v.29 no.1
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    • pp.43-52
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    • 2020
  • The objectives of this study were to identify the optimal mix of foam concrete with the low-strength and high-flow for the repairing ground subsidence situation emergently by utilizing a large amount of industrial by-products and evaluate the possibility by applying it to the site. The factors of the experiment were the mixing ratio of mixing water and a foaming agent and the mixing ratio of foam over paste volume. The optimal mix identified by the experiment was applied to the field and basic properties were evaluated. The results of the experiment showed that the optimal mixing ratio of mixing water and the foaming agent was 10%. Moreover, when the mixing ratio of pre-foam over paste volume was 170%, it satisfied the target. However, to ensure stable quality when applying to the field, the foam mixing ratio was set 140% for the field application. The field application test of foam concrete with the low-strength and high-flow using an eco-friendly binder satisfied all target performances. Therefore, the possibility of using it as a mixture and construction method for a ground repair system is confirmed. However, there was a quality deviation between the upper part and the lower part due to the separation between foam and paste. Consequently, further studies are needed to improve it.

A Study on Rust Cleaning of Various Industrial Equipment Using Cosmetic and Food Materials (화장품과 식품 재료를 이용한 각종 산업장비 녹(rust) 세정에 관한 연구)

  • Yeom, Seok-Jae;Jung, Sundo;Oh, Eunha
    • Journal of the Korean Applied Science and Technology
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    • v.38 no.1
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    • pp.19-28
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    • 2021
  • Corrosion is the degradation of metals by reaction with the environment. It is difficult to completely remove. Corrosion proceeds rapidly after the protective barrier is destroyed, and several reactions occur that alter the composition and properties of the metal surface and local environments, such as diffusion of metal cations into the matrix, the formation of oxides, and local pH changes. The study of corrosion of steel and iron is of theoretical and practical interest and is receiving considerable attention. Acid solutions, which are widely used in industrial pickling, acid descaling, cleaning and acidification of oil wells, require the use of corrosion inhibitors to suppress corrosion attacks on metallic materials. Physical removal of rust requires expensive special equipment, and chemical removal of it can cause corrosion or shorten the life of the metal. In this study, an eco-friendly rust cleaner was developed using cosmetics and food materials by applying the concept of perm reducing agent and chelate, and applied to remove rust from industrial and hot water pipes and various industrial devices. As a result, it was found that rust cleaners remove rust more effectively and safely compared to conventional treatment methods. At the same time, the rust removal efficiency was 1.75 to 2.5 times better for industrial piping and 1.56 to 2.2 times better for boiler hot water than conventional methods.

Research and Consideration of Eco-friendly Radiation Shielding using CT Contrast Agent (CT 조영제를 이용한 친환경적인 방사선 차폐에 관한 연구 및 고찰)

  • Sung-Gil Kim;Yeon-Sang Ji
    • Journal of the Korean Society of Radiology
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    • v.17 no.6
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    • pp.827-833
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    • 2023
  • CT(Computed Tomography) contrast agents are commonly used in general hospitals and university hospitals when taking radiographic examinations. The CT contrast medium contains a mixture of a substance called "Iodine", which absorbs radiation energy and makes it appear white in the CT image, further improving the image quality. In addition, the CT contrast agent, which moves like blood in the blood vessels, clearly differentiates it from muscle and water, so CT contrast agents are widely used in hospitals. These CT contrast agents absorb X-rays, but in order to absorb X-rays, they must have a high density or a high radiation absorption coefficient. Since the CT contrast agent is injected into the blood vessels, if the density is high, the blood vessels are strained and the patient is in shock. For this reason, it is necessary to match the density similar to that of water and always pay attention to side effects. In addition, the amount of CT contrast medium is adjusted according to the patient's body shape, and the remaining contrast medium is discarded. However, This study tried to find out the idea of recycling it as a radiation shielding material. Since the CT contrast medium has a high radiation absorption coefficient at a density similar to that of water, the amount to absorb radiation is adjusted, the amount of contrast medium and the amount of water are adjusted, and the amount of radiation absorbed is determined by mixing with water. In addition, a study was conducted to find out the result of the difference in radiation absorption in various ways by comparing the radiation quality coefficient and absorption coefficient with other substances or materials in an environmentally friendly method harmless to the human body by mixing CT contrast medium and water.

Screening of Antifungal Activities of Medicinal Plants for the Control of Turfgrass Fungal Disease (잔디 병해 방제를 위한 약용식물의 항균작용 탐색)

  • Kang, Jae Young;Kim, Dae Ho;Lee, Dong Gu;Kim, In Seob;Jeon, Min Goo;Lee, Jae Deuk;Kim, Ik Hwi;Lee, Sanghyun
    • Weed & Turfgrass Science
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    • v.2 no.1
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    • pp.70-75
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    • 2013
  • Seven medicinal plant extracts were tested for antifungal activities against six species of the major turfgrass pathogenic fungi (Colletotrichum graminicola, Pythium spp., Rhizoctonia cerealis, Rhizoctonia solani AG1-1, Rhizoctonia solani AG2-2, and Sclerotinia homoeocarpa) using paper disk diffusion method. Three medicinal plant extracts, including Pinus densiflora showed antifungal activities. In suppression of mycelium growth test, on medium adding P. densiflora extract showed that inhibition rate of mycelium growth were above 80% in 10 mg/10 ml concentration of the extract. The inhibition rate of Pythium spp. was 100% and C. graminicola was 84.3% in 10 mg/10 ml concentrations of P. densiflora extract, respectively. In particularly, the inhibition rate of Pythium spp. was 89.5% in 2 mg/10 ml concentrations of P. densiflora extract. As a result, P. densiflora extract showed high antifungal activity to Pythium spp. and C. graminicola of the turfgrass pathogen in in vitro test.

Characteristics and Virulence Assay of Entomopathogenic Fungus Metarhizium anisopliae for the Microbial Control of Spodoptera exigua (파밤나방의 생물적 방제를 위한 곤충병원성 곰팡이 Metarhizium anisopliae의 특성 및 병원성 검정)

  • Han, Ji Hee;Kim, Hyeonggyeong;Leem, Hun Tae;Kim, Jeong Jun;Lee, SangYeob
    • The Korean Journal of Pesticide Science
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    • v.17 no.4
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    • pp.454-459
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    • 2013
  • Beet armyworm, Spodoptera exigua is difficult to control using chemical insecticides because of the fast development of insecticide resistance. For eco-friendly beet armyworm managements, various control agents are required. Entomopathogenic fungus is one of the promise control agents as an alternative to chemical control agent. We isolated entomopathogenic fungi from soil samples of Yangpyeong, Gyeonggi-do by insect-bait method using Tenebio molitor and conducted bioassay to larva of beet armyworm. The result of bioassay, a selected strain FT83 showed 100% mortality against third instar larva of S. exigua. On the basis of morphological characteristics and analysis of 18srRNA sequence for ITS, the strain FT83 was identified as a Metarhizium anisopliae. The mortality of beet armyworm showed $81.6{\pm}9.3%$ at $1{\times}10^6$ conidia/ml, 100% at $1{\times}10^7$ conidia/ml and 100% at $1{\times}10^8$ conidia/ml respectively. Therefore, we recommend to proper control efficacy against S. exigua in which more than $1{\times}10^7$ conidia/ml suspension of M. anisopliae FT83.

Screening of Antifungal Medicinal Plants for Turfgrass Fungal Disease Control (잔디 병해 방제를 위한 항균성 약용식물의 탐색)

  • Kwon, Soo-Mean;Kim, Dae-Ho;Chang, Tae-Hyun;Jeon, Min-Goo;Kim, In-Seob;Kim, Ik-Hwi
    • Asian Journal of Turfgrass Science
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    • v.24 no.2
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    • pp.176-181
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    • 2010
  • Brown patch (Rhizoctonia solani AG1-1), dollar spot (Sclerotinia homoeocarpa), pythium blight (Pythium spp.), anthracnose (Colletotrichum graminicola), yellow patch (Rhizoctonia cerealis) and Zoysia patch (Rhizoctonia solani AG2-2) are the major turfgrasses diseases in Korea. In this study, 23 medicinal plant extracts were tested for antifungal activities against turfgrass pathogenic fungi. In paper disk diffusion method, 12 medicinal plant extracts, including Sophora flavescens, showed antifungal activity. Also, in the test of antifungal activity on media contained the extracts of S. flavescens, Curcuma longa, Rheum undulatum, Coptis chinensis and Asiasarum sieboldi showed above 80% inhibitory effects on the mycelial growth in 110 mg/10 ml concentration of the extracts. S. flavescens, in particularly, showed antifungal activity against the six turfgrass pathogenic fungi. The inhibition rate of S. homoeocarpa was 100% in 10 mg/10 ml, 5 mg/10 ml and 2 mg/10 ml concentrations of C. longa extract. In case of Pythium spp., the extracts of S. flavescens, R. undulatum and C. chinensis showed 100 % inhibition rate on the test media.

Morphological Characteristics and Classification of 25 Selected Clones of Aralia elata Seem (두릅나무 25개 선발 클론의 형태적 특성과 유연관계)

  • Kim, Sea Hyn;Kim, Moon Sup;Han, Jingyu;Kim, Hyeusoo;Moon, Heung Kyu
    • Korean Journal of Plant Resources
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    • v.26 no.1
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    • pp.36-43
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    • 2013
  • Aralia elata Seem. have a typical traditional significance among the wild herbs traditionally. Edible shoots of A. elata will augment consumer's interest due to its high value functional value, eco-friendly and pesticide-free produce. A. elata's root, fruit and bark are used as material of hypoglycemic agent and medicine for diabetes, kidney trouble, acute hepatitis, rheumation arthritis, stomach cancer and gastroenteric trouble. Flavonoid glycoside compound which is separated from A. elata's shoot shows high antioxidative activity. Also, root's identified active materials of antimicrobial was reported to be produced as food preservative and handy antimicrobial. Therefore, this research investigated quantitative morphological characteristics of leaves, spine and bud in naturally dominated and introducted A. elata in south Korea and then considered its principal compound analysis(PCA) and classification analysis(CA) among the 6 improved cultivars and 19 clones. PCA results showed that it show 76% accumulated explanation from four PC. The A. elata clones were classified into five groups; the first group of 15 clones including Yeongok, the second group of 5 clones including Yeoju, the third group of Bonghwa, Ulleung, the fourth group of Yongmunsa, Boseong and the fifth group of Singu. The object of this study will give us invaluable information about breeding by selection of A. elata in south Korea.

Basic Research of Self Compacting Concrete Using Alkali-Activated Slag Binder (알칼리 활성 슬래그 결합재를 이용한 자기충전 콘크리트의 기초 연구)

  • Song, Keum-Il;Shin, Gyeong-Sik;Gong, Min-Ho;Song, Jin-Kyu
    • Journal of the Korea Concrete Institute
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    • v.25 no.6
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    • pp.657-665
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    • 2013
  • The purpose of this study is the basic research of self-compacting concrete using Alkali-Activated Slag (AAS) binder in order to emphasize the durability of structures and facilitate casting the fresh concrete in field. The AAS binder emitted low carbon dioxide ($CO_2$) is eco friendly material of new concept because AAS products not only emit little $CO_2$ during production but also reuse the industrial by-products such as ground granulated blast-furnace slag (GGBS) of the steel mill. Until now, almost of domestic and foreign research are using Ordinary Portland Cement (OPC) for self-compacting concrete, and also, nonexistent research about AAS. The self-compacting concrete must get the performance of flowability, segregation resistance, filling and passing ability. Nine concrete mixes were prepared with the main parameter of unit amount of binder (400, 500, 600 $kg/m^3$) and 3 types of water-binder (W/B) ratio. The results of test were that fresh concretes were satisfied with flowability, segregation resistance, and filling ability of JSCE. But the passing ability was not meet the criteria of EFNARC because of higher viscosity of AAS paste than OPC. This high viscosity of AAS paste enables the manufacturing of self compacting concrete, segregation of which does not occur without the using of viscosity agent. It is necessary that the development of high fluidity AAS binders of higher strength and the study of better passing ability of AAS concrete mixes in order to use self compacting AAS concrete in field.

Antifungal Activity of Bacillus sp. GJ-1 Against Phytophthora capsici (Bacillus sp. GJ-1의 Phytophthora capsici에 대한 항진균활성)

  • Lee, Gun-Joo;Han, Joon-Hee;Shin, Jong-Hwan;Kim, Heung Tae;Kim, Kyoung Su
    • The Korean Journal of Mycology
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    • v.41 no.2
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    • pp.112-117
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    • 2013
  • Phytophthora capsici is one of major limiting factors in production of pepper and other important crops worldwide by causing foliage blight and rot on fruit and root. Increased demand for the replacement of fungicides has led to searching a promising strategy to control the fungal diseases. To meet eco-friendly agriculture practice, we isolated microorganisms and assessed their beneficial effects on plant health and disease control efficacy. A total of 360 bacterial strains were isolated from rhizosphere soil of healthy pepper plants, and categorized to 5 representative isolates based on colony morphology. Among the 5 bacterial strains (GJ-1, GJ-4, GJ-5, GJ-11, GJ-12), three bacterial strains (GJ-1, GJ-11, GJ-12) presented antifungal activity against P. capsici in an fungal inhibition assay. In phosphate solubilization and siderophore production, the strain GJ-1 was more effective than others. The strain GJ-1 was identified as Bacillus sp. using 16S rDNA analysis. Bacillus sp. GJ-1 was also found to be effective in inhibiting other plant pathogenic fungi, including Rhizoctonia solani, Pythium ultimum and Fusarium solani. Therefore, the Bacillus sp. GJ-1 can serve as a biological control agent against fungal plant pathogens.